• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
I`m the prokaryotic cell
I`m the prokaryotic cell

... From the biggest to the least My simplest form Is in everyday yeast ...
ppt
ppt

... interaction with various transition metal ions including Co2+, Ni2+, Cu2+ and Zn2+ ...
plasmodium protein kinases: from database mining to the search for
plasmodium protein kinases: from database mining to the search for

Chapter 6 Crossword Puzzle
Chapter 6 Crossword Puzzle

... What the body uses to assemble its own proteins Proteins that act to defend the body from disease Proteins that provide sufficient quantities of essential amino acids are referred to as _____. What happens when proteins are exposed to heat or acid such as stomach acid ...
Major Protein-sorting pathways in eukaryotic cells
Major Protein-sorting pathways in eukaryotic cells

... Major topological classes of integral membrane proteins synthesized on the rough ER ...
Database Modeling in Bioinformatics
Database Modeling in Bioinformatics

... • Manual annotation is best -especially if Medline number attached (biochemical evidence) • InterPro good, assuming protein hit is true and should hit all signatures in an entry • EC numbers good, but need mapping of protein to these, so may be extra step • SWISS-PROT keywords fine, but automatic an ...
A One- or Two-Day Course for Your Campus on
A One- or Two-Day Course for Your Campus on

... ligands, substrates, and drugs, and protein evolutionary conservation. Handson experience will be largely with molecules of each participant's choosing. Participants will learn easy methods for creating publication-quality molecular images, and how to put snapshots or rotating animations in Powerpoi ...
BIOMOLECULES-L2 students
BIOMOLECULES-L2 students

... intestines cannot break it down...and it passes through unchanged. ...
proteinszednii
proteinszednii

... • Pepsinogen is converted into the enzyme pepsin when it comes into contact with hydrochloric acid • Pepsin is the only proteolytic enzyme that digests collagen, the major protein of connective tissue ...
D5 Phylogeny and systematics – summary of mark
D5 Phylogeny and systematics – summary of mark

... Explain the biochemical evidence provided by the universality of DNA and protein structures for the common ancestry of living organisms. ...
Genetic Controls in Eukaryotes
Genetic Controls in Eukaryotes

... o Bind to promoter (TATA box); RNA Pol II can bind o “General” transcription factors leads to slow transcription. - General = essential to initiation of transcription of all protein o “Specific” transcription factors leads to faster transcription = Specific to transcription of particular protein. ...
How do proteins form turns? - UF Macromolecular Structure Group
How do proteins form turns? - UF Macromolecular Structure Group

... Helical regions are excluded from this definition (see later) Reverse turns are very abundant in globular proteins and generally occur at the surface of the molecule. It has been suggested that turn regions act as nucleation centres during protein folding ...
The macromolecular sites of action through which drugs
The macromolecular sites of action through which drugs

... hydrophobic residues to solvent, and may contain partially desolvated hydrophilic groups that are not paired with complementary hydrogen bonding residues. These hydrophilic groups in this area are probably not exposed to sufficient solvent due to the steric constraints of protein folding. This means ...
Test 2 - HCC Learning Web
Test 2 - HCC Learning Web

... Neethu Mathew ...
College 5
College 5

... Protein folding in a living cell is often assisted by special proteins called molecular chaperones. These proteins bind to partly folded polypeptide chains and help them progress along the energetically most favorable folding pathway. Chaperones are vital in the crowded conditions of the cytoplasm, ...
Biology Ch 10 How Proteins are Made
Biology Ch 10 How Proteins are Made

... • The genes don’t directly make proteins – A combined effort between 3 types of RNA ...
Chapter 3 - Proteins
Chapter 3 - Proteins

... • Name one polar and one nonpolar amino acid, then make a list of all the additional amino acids that you remember. • What are the four weak (noncovalent) interactions that determine the conformation of a protein? • (True/False) A protein is at a near entropy minimum (point of lowest disorder, or gr ...
Notes
Notes

... A tissue comprised of billions of cells heterozygous for BRCA1 or BRCA2 ...
Modifications of redox-active cysteines occurring during sample
Modifications of redox-active cysteines occurring during sample

... 80 Da, as well as 32, 48, 64, 76 and -34 Da as a result of sample preparation in protein expression and proteomic workflows. Different reasons have been elucidated and not all of them are fully clarified yet. 1) β-Mercaptoethanol is often used during the purification of expressed proteins (Begg & Sp ...
Nuclease Digestion
Nuclease Digestion

... • Amino acids = building blocks • 4 classes: – Basic – Polar ...
Bi-150-molbiol
Bi-150-molbiol

... A. Each chromosome is “painted” with a unique combination of fluorescent dyes ...
Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

... what that cell can do. ...
control biological machines
control biological machines

... • Control biological activity – external – reversible – on molecular scale (selective) – direct – in vitro/ in vivo – universal ...
DNA to Proteins
DNA to Proteins

... • Proteins are all through cells and make the differences you see in organisms. • Proteins are chemical triggers and messengers for cell processes. • An organism may have thousands for genes that code for thousands of proteins ...
Slide 1 - KU CTE
Slide 1 - KU CTE

... A fatty acid with C=C double bonds in the hydrocarbon chain ...
< 1 ... 460 461 462 463 464 465 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report