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DNA Unit Test Corrections
DNA Unit Test Corrections

... 30. What is the normal function of the protein you named in questions #29?________________ ____________________________________________________________________________ ____________________________________________________________________________ 31. What are some other functions of proteins in your b ...
Bioinformatics and Functional Genomics, Chapter 8, Part 1
Bioinformatics and Functional Genomics, Chapter 8, Part 1

... individual proteins. ExPASy and ISREC are two excellent resources. The accuracy of these programs is variable. Predictions based on primary amino acid sequence (such as molecular weight prediction) are likely to be more trustworthy. For many other properties (such as posttranslational modification o ...
1.16891 / 1.16886 Fractogel® EMD COO
1.16891 / 1.16886 Fractogel® EMD COO

... 1.16891 / 1.16886 Fractogel® EMD COO- (S), (M) Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and che ...
Biol 178 Lecture 4
Biol 178 Lecture 4

... A distinctive, usually recurrent structural element (secondary protein structures) such as a simple protein motif consisting of two alpha helices. ...
NF1X - BioMed Central
NF1X - BioMed Central

... adenoviral DNA [2]. It is highly conserved in vertebrates, with chicken and hamster orthologs showing 92% amino acid sequence identity [3]. NF1X is reported to control the expression of a number of different genes in liver [4-8], and is a known repressor of glutathione S-transferase [9], which is in ...
Unit 1 Rev 2 - Mr. Lesiuk
Unit 1 Rev 2 - Mr. Lesiuk

... ___ 2. Name the three main nutrient groups/chemicals used by cells. ___ 3. What are the basic building blocks that make up a protein molecule? ___ 4. Many of the proteins/enzymes that a cell makes are crucial for the cell to properly work, what does the cell have (use) in order to help it build thes ...
CH 3 RG 2014 Carbon and the Molecular Diversity of Life
CH 3 RG 2014 Carbon and the Molecular Diversity of Life

... are nonpolar, some polar, and others electrically charged (acidic or basic). If you were given an R group, could you place it in the correct group? Work the R groups until you can see common elements in each category. ...
Hemoglobin, or haemoglobin, is an iron
Hemoglobin, or haemoglobin, is an iron

... Hemoglobin (Hb) is an iron-containing protein in the red blood cells of mammals and other vertebrates. Hb is released under certain pathological conditions, such as malarial infection and allergic drug reaction. This free Hb is toxic and causes damage to blood vessels and surrounding tissues. Haptog ...
Exam 1 Review KEY
Exam 1 Review KEY

... 10.) A molecule that is a mirror image of another, having a key functional group oriented in a different direction, is called a ___stereoisomer________ of the other. 11.) What forms do humans and plants store glucose as? Humans – glycogen Plants – starch 12.) How many double bonds do the following f ...
chapter_6_-_plus_ch_review
chapter_6_-_plus_ch_review

... 6. What is meant by the term “essential amino acid”? How many amino acids are essential? What are their names? (You won’t have to know these for exam) 7. What are some of the ways a protein is rated? (Describe at least 2 – tell what they are measuring) 8. How much protein do you need according to th ...
9B2.1 anti-glass INVESTIGATOR Name Gerald M. Rubin
9B2.1 anti-glass INVESTIGATOR Name Gerald M. Rubin

... Glass Ag in tissues is apparently methanol sensitive. Immunoblotting Purification Amino acid sequence analysis Functional effects Immunohistochemistry PUBLICATIONS : Ellis, M.C., O'Neill, E.M., and Rubin, G.M. (1993). Expression of Drosophila glass protein and evidence for negative regulation of its ...
2. Purification of WDR77
2. Purification of WDR77

Proteins Hwk KEY
Proteins Hwk KEY

Jan. 28 Bio II Answer to warm up Protein Synthesis
Jan. 28 Bio II Answer to warm up Protein Synthesis

... codons each When a tRNA bumps into the ribosome that has the complimentary sequence to a codon, the tRNA is trapped and fit into place. The bond between the tRNA and the amino is broken and the amino acid is now held by the ...
Biologically Important Molecules - Proteins PPT
Biologically Important Molecules - Proteins PPT

... Levels of Protein Organization ...
Protein Synthesis PPT
Protein Synthesis PPT

organic compounds outline
organic compounds outline

... used in protein  function of individual proteins  _____________________ – copying the DNA gene to a strand of mRNA  ____________________ – ribosomes assemble amino acids into the correct sequence  Knows the sequence by the mRNA code  Problems – __________________  Def: changes in the DNA seque ...
Study Guide
Study Guide

... 3. DNA contains the genetic code. It is a double stranded molecule that has a double helix structure. Deoxyribose is the sugar that makes up this molecule. DNA is contained in the nucleus of the cell. 4. The genetic code is the order of the nitrogen bases that form along a gene and directs what type ...
Proteins
Proteins

... of amino acids can have 20300 different linear arrays of aa's [10390 different proteins] ...
Protocol S3 – Proteomic analysis
Protocol S3 – Proteomic analysis

... but it was outperformed by the logistic regression model (see Table S3). The trained logistic regression models based on the gold standards were then used to assign a single probability value to each of the putative PIs generated in the study. The CP score provides the relative likelihood of each po ...
Biology Topics, Venn diagrams
Biology Topics, Venn diagrams

DNA Function II - Complete Vocab with
DNA Function II - Complete Vocab with

... General Transcription Factors: Other enzymes/proteins that are required for RNA Polymerase to function Transcription Activators: Proteins that bind to enhancers to stimulate transcription Transcription Repressors: Proteins that bind to enhancers to shut down transcription Enhancer: A sequence of DNA ...
Distinguish between these 3 root types: - mvhs
Distinguish between these 3 root types: - mvhs

... Ribosome– The “workbench” where translation takes place. Catalyzes the formation of __________ bonds between amino acids to form the polypeptide chain. Made of ________ and rRNA. ...
Biochemistry Jeopardy C.P. Bio.
Biochemistry Jeopardy C.P. Bio.

... The disaccharide named Maltose is formed by chemically bonding two of these monosaccharides. ...
receptor proteins
receptor proteins

... accomplishes  this  feat  –  despite  the  fact  that  our  lives  depend  on  exactly  this  fantastic  feature.   Understandably many science groups have been working on this topic – but many in vain. The analyses of  the  proteins’  primary,  secondary  and  tertiary  structure  gives  a  vast  a ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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