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Chapter 5: Biological Molecules Molecules of Life • All life made up
Chapter 5: Biological Molecules Molecules of Life • All life made up

... o Polymer built from set of 20 amino acids o Linked by peptide bonds via dehydration reaction o Each has unique amino acid sequence; can be a few to more than a thousand  Amino Acid Structure o -Carbon bonded to:  Hydrogen  Carboxyl group  Amino group  Side Chain (R group) – accounts for diffe ...
A Novel Framework for De Novo Protein Design and its Applications
A Novel Framework for De Novo Protein Design and its Applications

... acid sequences with the lowest energies by solving an integer programming sequence selection model [1]. The second stage employs Monte Carlo simulations to predict the structures [2] of the sequences from stage one and to perform docking simulations [3] between the new sequences and the target prote ...
Chapter 3 (part 2) – Protein Function
Chapter 3 (part 2) – Protein Function

... • Enzymes and bound ligand go through a number of intermediate forms of different geometry. They are all called transition states. • The energy that it takes to get to the most unstable transition state is called the activation energy. • Enzymes speed reactions by selectively stabilizing the transi ...
charge-to-mass ratio. The electrophoretic mobility is defined as the
charge-to-mass ratio. The electrophoretic mobility is defined as the

Gene Expression
Gene Expression

Gene Expression
Gene Expression

... All levels of transcription and translation are involved: 1. DNA sequence will encode for specific regulation – promoters, exons/introns, etc 2. RNAs – will affect which genes complete the process to become proteins 3. Proteins – function as enzymes and machinery to activate or silence specific gene ...
Answers-to-examination-in-Gene-technology_20121020
Answers-to-examination-in-Gene-technology_20121020

... Change in the DNA sequence that do not cause any change in the amino acid sequence. e) A palindromic sequence: CTTTGA change to 5’-CTATAG-3’ or 5’-TTATAA-5 3’-GATATC-5’ 3’-AATATT-3’ f) The advantage is the possibility to regulate the transcription of the gene. If the gene product is toxic and harmfu ...
TRANSCRIPTION and TRANSLATION
TRANSCRIPTION and TRANSLATION

... Draw a corresponding tRNA with an amino acid attached to it. Show how the tRNA attaches to the mRNA and how the rest of the tRNA molecules attach to the mRNA and how the amino acids link together. ...
Template to create a scientific poster
Template to create a scientific poster

... Figure 4: Three-dimensional representation of the HSPA1A protein molecule showing the position of the mutations. The ATPase domain (blue) and substrate-binding domain (green) are connected by the hydrophobic linker (red). Inlets show a magnification of the WT amino acid residues (yellow) and the mut ...
The subcomponents of biological molecules and their sequence
The subcomponents of biological molecules and their sequence

... Protein’s property is really dependent on its subcomponents; for protein everything matters. The R group is vital in proteins because the R groups of amino acids are the components that make the final shape of the protein and the function of the protein. The bonding in the tertiary structure is all ...
Transcription and Translation
Transcription and Translation

...  I.e. difference in enzymes (make different amounts of molecules)  I.e. difference in antibodies (some get sick more often or from different things) ...
A.D.Hershey and Martha Chase (1952). Independent Function of
A.D.Hershey and Martha Chase (1952). Independent Function of

... still remained as to which of the two conferred heritable information. 1928- Frederick Griffith - showed that heat killed virulent bacteria can transform a non-virulent strain. 1944- Avery, MacLeod, McCarty - report they have isolated the transforming principle in Griffiths experiment and that the p ...
Gene Section JARID1A (jumonji, AT rich interactive domain 1A (RBBP2-like))
Gene Section JARID1A (jumonji, AT rich interactive domain 1A (RBBP2-like))

... From centromere to telomere, yielding mRNA of 6,5 kb. ...
GoMap
GoMap

... -CGDc cell cycle & division -CGDg cell growth & development -CGDd cell death ...
Protein Synthesis
Protein Synthesis

... 7. Working one code at a time, make your protein (your chain of amino acids) using the beads and pipe cleaners. When you have your protein completed, have your teacher check it. If there are any errors, please go back and find your mistakes. 8. Did you have any “mutations” during the process? _____ ...
Transcription and Translation computer lab test review
Transcription and Translation computer lab test review

... During transcription, RNA is developed from a strand of DNA. List the base pairs used to make RNA. What is the name of the DNA strand used in transcription? Where does transcription occur? Where does translation occur? Name the RNA codon that is used to start translation. Which three codons will sto ...
Lecture 2: Biological Side of Bioinformatics
Lecture 2: Biological Side of Bioinformatics

... Caused by reproduction and survival of the fittest Organism has to live with it (or die before reproduction) Three mechanisms: inheritance, mutation and crossover ...
Reading guide
Reading guide

1101Lecture 16 powerpoint
1101Lecture 16 powerpoint

1101Lecture 24 powerpoint
1101Lecture 24 powerpoint

... Food labels will allow you to do this Following Canada’s food guide will allow you to estimate this ...
Document
Document

... WHAT KEY KNOWLEDGE, SKILLS, AND ATTITUDES WILL STUDENTS ACQUIRE AS A RESULT OF THIS UNIT? ObjectivesStudents will understand: Students will be able to: (1)The mechanisms by which our immune system fights pathogens. (2)Mechanism of virus/host cell interaction and transmission. ...
energy currency for cell - Hermantown Community Schools
energy currency for cell - Hermantown Community Schools

SBI 4U Genetics 5
SBI 4U Genetics 5

... repressor protein and changes the shape of it so it can no longer bind onto the operator. Then the CAP activator can bind on and controls how fast the transcription works. The lac operon is known as an inducible operon because transcription is induced when lactose is present. ...
CHAPTER 6
CHAPTER 6

... Working draft of human genome reported by 2 groups allowed estimates that genome contains fewer genes than anticipated – 25,000 to 40,000 About half the genome has derived from the action of transposons Transposons themselves have contributed dozens of genes to the ...
protein synthesis (simplified)
protein synthesis (simplified)

... DNA is a very long molecule that looks like a twisted ladder. It is made up of 4 different subunits called nucleotides which can be arranged in any order ...
< 1 ... 455 456 457 458 459 460 461 462 463 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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