• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Leukaemia Section t(1;14)(q21;q32) IRTA1/IGH Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(1;14)(q21;q32) IRTA1/IGH Atlas of Genetics and Cytogenetics in Oncology and Haematology

... transcripts of 2.5kb, 2.7kb and 3.5kb are possible due to alternate usage of 3 polyadenylation sites. Protein The three alternate IRTA1 transcripts give rise to the same putative 515 amino acid protein. The protein shows a signal peptide, four extracellular Ig-type domains carrying three potential a ...
Cell and Molecular Biology
Cell and Molecular Biology

... • Structural: offering stiffness and rigidity to fluid biological components. Collagen is the most abundant structural protein in mammals or Scafold proteins. • Catalysis of chemical reactions as enzymes. Only a small region of an enzyme called the active site binds the substrate and contains the c ...
Carriers of Disease
Carriers of Disease

... • When normal cell is transformed into prion the amino acids are folded into alpha helical structures and relax into beta sheets • Prion then clog cells which decreases function or stops the cell from functioning all together • In brain tissue prion-bloated brain cells dies and release prion into t ...
Unit 3 Biology - moleculesoflife2
Unit 3 Biology - moleculesoflife2

... The shape into which a protein naturally folds is known as its native state, which is determined by its sequence of amino acids. Thus proteins are known as polymers, with amino acids being the monomers. Biochemists refer to four distinct aspects of protein structure. Primary structure. – The amino a ...
Protein Purification and Analysis Day 4
Protein Purification and Analysis Day 4

... at the pH of the running buffer. This charge will, of course, depend on the amino acid composition of the protein as well as post-translational modifications such as addition of sialic acids. Since the protein retains its folded conformation, its hydrodynamic size and mobility on the gel will also v ...
A1980JC93500001
A1980JC93500001

... polymer chains (‘random coils’), and the sequence/structure/function hypothesis was proved. The original work (which, I should mention, ultimately involved several students and postdoctoral fellows in addition to Kawahara and Lapanje) was published in about a dozen papers. I summarized what we had l ...
protein - 4J Blog Server
protein - 4J Blog Server

... • The four structural levels of proteins and how changes at any level can affect the activity of the protein. • How proteins reach their final shape (conformation), the denaturing impact that heat and pH can have on protein structure, and how these may affect the organism. • The directionality of pr ...
Proteins Quiz - cloudfront.net
Proteins Quiz - cloudfront.net

... Proteins Quiz 1. Roughly how many amino acids are present in a polypeptide? a) 5-10 b) 10-100 c) 30-60 d) more than 80 ...
Bioinformatics
Bioinformatics

... Protein 3-D structure • Relationship between sequence & structure • Secondary structure – Alpha helix – Beta sheet ...
Supplementary Figure Legends (doc 60K)
Supplementary Figure Legends (doc 60K)

... Benjamini–Hochberg multiple test correction shows 39 differentially expressed genes in CRC and NSCLC cell lines according to their sensitivity or resistance to selumetinib. ...
Translation
Translation

... In order for a cell to make a protein, DNA must be transcribed into ____________ and the mRNA must then be translated into _____________. The processes of transcription and translation together are called _________________________. The process of transcription occurs in the ____________ of a cell. T ...
The Leucine Binding Fluorescence Analysis of the Leucine Specific
The Leucine Binding Fluorescence Analysis of the Leucine Specific

... These proteins are in the periplasmic space of bacteria such as E.coli. Bacterial periplasmic substrate binding proteins act as initial receptors for transport, chemotaxis, antibiotic resistance, and energy utilization. There are more than 40 proteins that are classified as periplasmic binding prote ...
sg 10
sg 10

... 24. Distinguish between a point mutation and a frameshift mutation. Which would be more severe? ...
AP Biology
AP Biology

... 24. Distinguish between a point mutation and a frameshift mutation. Which would be more severe? ...
Lecture 1: Fundamentals of Protein Structure
Lecture 1: Fundamentals of Protein Structure

... Amino Acids Are Joined By Peptide Bonds In Peptides - -carboxyl of one amino acid is joined to -amino of a second amino acid (with removal of water) - only -carboxyl and -amino groups are used, not R-group carboxyl or amino groups ...
✓ 10 FOLLOW-UP
✓ 10 FOLLOW-UP

... mRNA. You might also ask them to make up their own sequences to work from. Be sure the sequences they select include a start codon. Analysis Question 4 is a Quick Check to assess students’ understanding that DNA mutations might or might not have an effect on transcription and translation, depending ...
Protein Synthesis
Protein Synthesis

... amino acid proline. A long string of amino acids forms a protein. Each gene is usually a set of instructions for making a protein. Proteins are responsible for most of the differences in organisms that we see. EX: height, curly or straight hair, etc. ...
02 DNA and RNA and protein synthesis
02 DNA and RNA and protein synthesis

... When two are there, one will attach its amino acid to the amino acid of the other one, creating a chain of amino acids. Once this happens, that tRNA leaves and the one with the chain moves to its place. ...
D. E. Shaw Research is seeking postdoctoral fellows of exceptional... Postdoctoral Fellowships at D. E. Shaw Research
D. E. Shaw Research is seeking postdoctoral fellows of exceptional... Postdoctoral Fellowships at D. E. Shaw Research

... D. E. Shaw Research is seeking postdoctoral fellows of exceptional ability to join our New York–based team. This is a unique opportunity to develop and work with transformative technology in a dynamic, interdisciplinary environment. Candidates should have expertise in computational chemistry, biolog ...
macromolecule_sheets
macromolecule_sheets

... 1. What types of atoms make up carbohydrates? 2. How does the number of H atoms in a carbohydrate compare to the number of oxygen atoms? 3. What is the simplest type of carbohydrate? 4. What two functional groups are found in monosaccharides? 5. Name three common monosaccharides and give the molecul ...
04-05 Biochem review sheet answers ws
04-05 Biochem review sheet answers ws

... 17. Explain the difference between a saturated and unsaturated fatty acid. Unsaturated have some double carbon bonds. 18. List some of the many functions of proteins. transport, hormones, receptors, defense, etc. 19. How do amino acids differ? R group 20. What is a peptide bond? covalent bond betwee ...
Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... Protein Trafficking & Cell-cell communications Criticisms & Conclusion ...
Heat shock proteins
Heat shock proteins

Standard Genetic Code
Standard Genetic Code

File - Mrs. Loyd`s Biology
File - Mrs. Loyd`s Biology

... 2. Give an example of local regulation with some details. 3. Why are hormones considered long-distance signalers? 4. Earl Sutherland discovered how the hormone epinephrine (adrenaline) acts on cells. Use this as an example to illustrate the three stages of cell signaling: reception, transduction and ...
< 1 ... 451 452 453 454 455 456 457 458 459 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report