Chaperone-dependent gene expression of organic
... 3.7. Phylogenetic analysis of S5 lipase The G + C content of a gene encoding a protein may reveal the origin of the protein. The G + C content was calculated using FramePlot 2.3.2 software available at http://www.watson.nih.go.jp. The G + C content of this organic solvent-tolerant lipase gene was 65 ...
... 3.7. Phylogenetic analysis of S5 lipase The G + C content of a gene encoding a protein may reveal the origin of the protein. The G + C content was calculated using FramePlot 2.3.2 software available at http://www.watson.nih.go.jp. The G + C content of this organic solvent-tolerant lipase gene was 65 ...
Engineering of cyclodextrin glycosyltransferase reaction and
... need for a solvent recovery process [15]. The availability of K- and Q-cyclodextrins is thus rather limited at present; consequently, there is a great demand for a process that could produce these cyclodextrins economically. Also the processes used for L-cyclodextrin production are not ideal, since ...
... need for a solvent recovery process [15]. The availability of K- and Q-cyclodextrins is thus rather limited at present; consequently, there is a great demand for a process that could produce these cyclodextrins economically. Also the processes used for L-cyclodextrin production are not ideal, since ...
Unconventional serine proteases: Variations on the catalytic Ser/His
... Chymotrypsin- and trypsin-like proteases Two of the best-known serine proteases that utilize the Ser/His/Asp triad are chymotrypsin and trypsin. They share the same protein fold and have their catalytic residues in the order of His/Asp/Ser from the N to C terminus. Their active site regions are comp ...
... Chymotrypsin- and trypsin-like proteases Two of the best-known serine proteases that utilize the Ser/His/Asp triad are chymotrypsin and trypsin. They share the same protein fold and have their catalytic residues in the order of His/Asp/Ser from the N to C terminus. Their active site regions are comp ...
patrick_tb_ch17
... Type: multiple choice question Title: Chapter 17 Question 28 28) What feature of the protease active site permits a hybridisation strategy to be used in the design of protease inhibitors? Feedback: Since the active site is symmetrical, inhibitors can bind 'left to right' or 'right to left'. Since th ...
... Type: multiple choice question Title: Chapter 17 Question 28 28) What feature of the protease active site permits a hybridisation strategy to be used in the design of protease inhibitors? Feedback: Since the active site is symmetrical, inhibitors can bind 'left to right' or 'right to left'. Since th ...
Factor IXHollywood
... The mutation identified above is located in the first EGF-like domain of factor IX. This mutation creates recognition sites for four restriction enzymes ( N s i l . Avull. Nsp75241, and NspHI) as determined by the IRI Pustell Sequence Analysis Program. version 2.02 (International Riotcchnologia. Inc ...
... The mutation identified above is located in the first EGF-like domain of factor IX. This mutation creates recognition sites for four restriction enzymes ( N s i l . Avull. Nsp75241, and NspHI) as determined by the IRI Pustell Sequence Analysis Program. version 2.02 (International Riotcchnologia. Inc ...
(pdf)
... where tcell is in years, V is the sustaining metabolic rate estimated from geochemical constraints in moles of reactant L1 year1, Ygrowth is the growth yield in grams dry weight of biomass (mole of reactant)1, Ccell is the concentration of microbial cells L1, and mcell is the average cell mass i ...
... where tcell is in years, V is the sustaining metabolic rate estimated from geochemical constraints in moles of reactant L1 year1, Ygrowth is the growth yield in grams dry weight of biomass (mole of reactant)1, Ccell is the concentration of microbial cells L1, and mcell is the average cell mass i ...
Investigation of asparagine deamidation in a SOD1
... shift of -17 Da can be indicative of succinimide intermediate whose existence correlates with formation of Asp/isoAsp isomers and therefore may be useful in identification of the deamidation site. There are also two indirect proofs for the presence of isoAsp residue employed in this study: terminati ...
... shift of -17 Da can be indicative of succinimide intermediate whose existence correlates with formation of Asp/isoAsp isomers and therefore may be useful in identification of the deamidation site. There are also two indirect proofs for the presence of isoAsp residue employed in this study: terminati ...
Gluconeogenesis, Glycogen Metabolism, and the Pentose
... The starting material oxaloacetate is generated in the mitochondria from amino acid catabolism or it is drawn out of the TCA cycle to slow the pathway. The oxaloacetate is converted to malate by the action of the mitochondrial isoenzyme of Malate Dehydrogenase, the TCA cycle enzyme running in the re ...
... The starting material oxaloacetate is generated in the mitochondria from amino acid catabolism or it is drawn out of the TCA cycle to slow the pathway. The oxaloacetate is converted to malate by the action of the mitochondrial isoenzyme of Malate Dehydrogenase, the TCA cycle enzyme running in the re ...
Standard for the presentation of nucleotide and amino acid
... Only numeric identifiers of data elements as defined in Appendix 1 shall be used for the presentation of the items of information in the sequence listing. The corresponding numeric identifier descriptions shall not be used. The provided information shall follow immediately after the numeric identifi ...
... Only numeric identifiers of data elements as defined in Appendix 1 shall be used for the presentation of the items of information in the sequence listing. The corresponding numeric identifier descriptions shall not be used. The provided information shall follow immediately after the numeric identifi ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
... prostaglandin E2 production and increased expression of cyclooxygenase mRNA and protein (Ballou et al. ’92). While the role of ceramide as a signaling molecule in response to stress has been well studied in mammalian systems there is little information on the function and metabolism of ceramide in ...
... prostaglandin E2 production and increased expression of cyclooxygenase mRNA and protein (Ballou et al. ’92). While the role of ceramide as a signaling molecule in response to stress has been well studied in mammalian systems there is little information on the function and metabolism of ceramide in ...
Pathways to abscisic acid-regulated gene expression
... 2000). DET1 encodes a nuclear-localized protein that when mutated results in severe defects in the temporal and spatial regulation of light-regulated and developmental (e.g. ABI3) genes. The CO gene encodes a protein with homology to zinc-finger transcription factors that promotes flowering (Putteri ...
... 2000). DET1 encodes a nuclear-localized protein that when mutated results in severe defects in the temporal and spatial regulation of light-regulated and developmental (e.g. ABI3) genes. The CO gene encodes a protein with homology to zinc-finger transcription factors that promotes flowering (Putteri ...
CHAPTER 15 - GLYCOGEN METABOLISM AND
... Upon hormonal binding, c-AMP is formed which binds to the regulatory (R) subunit of cAPK, relieving the inhibition of the C subunit. activated cAPK then simultaneously phosphorylates phosphorylase kinase and glycogen synthase, activating and deactivating them, respectively (note color switch). cAPK ...
... Upon hormonal binding, c-AMP is formed which binds to the regulatory (R) subunit of cAPK, relieving the inhibition of the C subunit. activated cAPK then simultaneously phosphorylates phosphorylase kinase and glycogen synthase, activating and deactivating them, respectively (note color switch). cAPK ...
What Are Next Generation Innovative Therapeutic Targets?
... positive phase III results. This method dropped 89% of the 19 discontinued clinical trial targets and 97% of the 65 targets failed in high-throughput screening or knockout studies. Collective consideration of multiple profiles demonstrated promising potential in identifying innovative targets. ...
... positive phase III results. This method dropped 89% of the 19 discontinued clinical trial targets and 97% of the 65 targets failed in high-throughput screening or knockout studies. Collective consideration of multiple profiles demonstrated promising potential in identifying innovative targets. ...
Neighbor-Dependent Ramachandran Probability Distributions of
... The three-dimensional structure of a protein enables it to perform its specific function, which may be catalysis, DNA binding, cell signaling, maintaining cell shape and structure, or one of many other functions. Predicting the structures of proteins is an important goal of computational biology. On ...
... The three-dimensional structure of a protein enables it to perform its specific function, which may be catalysis, DNA binding, cell signaling, maintaining cell shape and structure, or one of many other functions. Predicting the structures of proteins is an important goal of computational biology. On ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... (nucleotides 1030–1124 in Escherichia coli) next to the protein L11 at the base of the stalk (3,4). L12 is the only multicopy protein on the ribosome (5), its number varying from species to species (1). In E. coli, two L12 dimers and one L10 protein form a strong pentameric complex known as the L8 c ...
... (nucleotides 1030–1124 in Escherichia coli) next to the protein L11 at the base of the stalk (3,4). L12 is the only multicopy protein on the ribosome (5), its number varying from species to species (1). In E. coli, two L12 dimers and one L10 protein form a strong pentameric complex known as the L8 c ...
Plant Peroxisomes: Biogenesis and Function
... identified a set of peroxins encoded by PEX genes that are required for peroxisome biogenesis. The growth and division paradigm was challenged by demonstrations that mutant yeast and mammalian cells lacking certain PEX genes, such as PEX3 and PEX19, were devoid of any obvious peroxisomal structures, ...
... identified a set of peroxins encoded by PEX genes that are required for peroxisome biogenesis. The growth and division paradigm was challenged by demonstrations that mutant yeast and mammalian cells lacking certain PEX genes, such as PEX3 and PEX19, were devoid of any obvious peroxisomal structures, ...
Myr 8, A Novel Unconventional Myosin Expressed during Brain
... performed using probe 4, which was generated by PCR amplification using the K IAA0865 specific antisense primer as described below for PCR amplification of myr 8 isoforms. Probe 4 represents nucleotides 3905– 4600 of the myr 8b sequence. Northern blot anal yses. Total RNA was extracted from primary ...
... performed using probe 4, which was generated by PCR amplification using the K IAA0865 specific antisense primer as described below for PCR amplification of myr 8 isoforms. Probe 4 represents nucleotides 3905– 4600 of the myr 8b sequence. Northern blot anal yses. Total RNA was extracted from primary ...
Characterization of the chimeric seven
... Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine partial genes of PR homologues using polymerase chain reaction ...
... Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine partial genes of PR homologues using polymerase chain reaction ...
What is a Multiple Alignment?
... alignment, and now I’d like you to correct it! There are two groups of sequences and one single sequence to adjust. ...
... alignment, and now I’d like you to correct it! There are two groups of sequences and one single sequence to adjust. ...
The promiscuous primase
... DNA primases are essential for the initiation of DNA replication and progression of the replication fork. Recent phylogenetic analyses coupled with biochemical and structural studies have revealed that the arrangement of catalytic residues within the archaeal and eukaryotic primase has significant s ...
... DNA primases are essential for the initiation of DNA replication and progression of the replication fork. Recent phylogenetic analyses coupled with biochemical and structural studies have revealed that the arrangement of catalytic residues within the archaeal and eukaryotic primase has significant s ...
Construction of in vivo infectious clones of PRSV P-YK and W-CI
... fragments of pT3PYKFN1 with the corresponding segments from pT3WCIF1, respectively. The clones p35SP-WCINIa, p35SP-WCI6k, and p35SP-WNIa were constructed by replacing the NheI-SacI, NheI-NsiI, and NsiI-SacI fragments of p35SPYK with the corresponding segments from p35SWCI, respectively. The type W r ...
... fragments of pT3PYKFN1 with the corresponding segments from pT3WCIF1, respectively. The clones p35SP-WCINIa, p35SP-WCI6k, and p35SP-WNIa were constructed by replacing the NheI-SacI, NheI-NsiI, and NsiI-SacI fragments of p35SPYK with the corresponding segments from p35SWCI, respectively. The type W r ...
1 Regulation of Substrate Oxidation Preferences in Muscle by the
... (1; 2). These studies linked adropin actions to the control of lipid glucose metabolism, however the exact role remains unclear (1; 2). Adropin is mainly expressed in liver (1), and its expression in this organ is stimulated by feeding and suppressed by fasting (1; 2). Plasma adropin concentrations ...
... (1; 2). These studies linked adropin actions to the control of lipid glucose metabolism, however the exact role remains unclear (1; 2). Adropin is mainly expressed in liver (1), and its expression in this organ is stimulated by feeding and suppressed by fasting (1; 2). Plasma adropin concentrations ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.