Substrate Specificity of Human Kallikreins 1 and 6
... peptides that had been used for the analysis of the specificity of KLK1 mentioned above. Specifically, Angelo et al. reported that only Arg is accepted in the S1 subsite of KLK6 whereas S2 strongly prefers Phe and, to a lesser extent Leu, over all other amino acids (Angelo et al. 2006). The determin ...
... peptides that had been used for the analysis of the specificity of KLK1 mentioned above. Specifically, Angelo et al. reported that only Arg is accepted in the S1 subsite of KLK6 whereas S2 strongly prefers Phe and, to a lesser extent Leu, over all other amino acids (Angelo et al. 2006). The determin ...
Lactobacillus plantarum - UEF Electronic Publications
... environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, makin ...
... environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, makin ...
New lysosomal acid lipase gene mutants explain the phenotype of
... cholesteryl ester and triglycerides within the range of experimental error (,5%) (4). However, the mutant proteins showed a variable proportion of N-glycosylation, which we have recently found to be critical for a full catalytic activity of normal LAL (5). In order to detect more accurately the prob ...
... cholesteryl ester and triglycerides within the range of experimental error (,5%) (4). However, the mutant proteins showed a variable proportion of N-glycosylation, which we have recently found to be critical for a full catalytic activity of normal LAL (5). In order to detect more accurately the prob ...
YangSpr07
... RGD peptides are short peptide fragments derived from the amino acid sequence of several extracellular matrix proteins, such as fibrinogen, fibronectin, vitronectin, collagen, and laminin. The amino acid sequence Arg-Gly-Asp or RGD present on extracellular matrix proteins is known to be a requiremen ...
... RGD peptides are short peptide fragments derived from the amino acid sequence of several extracellular matrix proteins, such as fibrinogen, fibronectin, vitronectin, collagen, and laminin. The amino acid sequence Arg-Gly-Asp or RGD present on extracellular matrix proteins is known to be a requiremen ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
... coded by a single gene (CACZ), which i s interrupted by 15 introns. The cDNA encodes a deduced protein of 537 amino acids with an apparent N-terminal chloroplast-targeting transit peptide. Antibodies generated to a glutathione S-transferase-CAC2 fusion protein react solely with a 51-kD polypeptide o ...
... coded by a single gene (CACZ), which i s interrupted by 15 introns. The cDNA encodes a deduced protein of 537 amino acids with an apparent N-terminal chloroplast-targeting transit peptide. Antibodies generated to a glutathione S-transferase-CAC2 fusion protein react solely with a 51-kD polypeptide o ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
Document
... The catalytic cycle of enzyme action can be described in the following steps. a. First the substrate binds to the active site of the enzyme fitting into the active site. b. the binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate c. the active si ...
... The catalytic cycle of enzyme action can be described in the following steps. a. First the substrate binds to the active site of the enzyme fitting into the active site. b. the binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate c. the active si ...
REVIEWS
... global regulators. Recent work has established that some of these global regulators are also integrated into a larger regulatory scheme by which the cell (Bacillus subtilis in this Review) coordinates the flow through key metabolic intersections in response to a small number of specific signalling m ...
... global regulators. Recent work has established that some of these global regulators are also integrated into a larger regulatory scheme by which the cell (Bacillus subtilis in this Review) coordinates the flow through key metabolic intersections in response to a small number of specific signalling m ...
Structure and Function of Large Bio Molecules
... An immense variety of polymers can be built from a small number of monomers. • Each cell has thousands of different kinds of macromolecules. • Macromolecules vary among cells of the same individual. They vary more among unrelated individuals of a species, and even more between species. • This divers ...
... An immense variety of polymers can be built from a small number of monomers. • Each cell has thousands of different kinds of macromolecules. • Macromolecules vary among cells of the same individual. They vary more among unrelated individuals of a species, and even more between species. • This divers ...
Bioinformatics Explained
... which can be changed in order to obtain the best possible result. Changing these parameters can have a great impact on the search result. It is not the scope of this document to comment on all of the options available but merely the options which can be changed with a direct impact on the search res ...
... which can be changed in order to obtain the best possible result. Changing these parameters can have a great impact on the search result. It is not the scope of this document to comment on all of the options available but merely the options which can be changed with a direct impact on the search res ...
Pepsinogen and Pepsin - The Journal of General Physiology
... mixtures were analyzed in this work by Kern, the nature of the results suggests that the three disulfide bridges reduce at such different rates that it amounts to a specific reduction with little overlap (see Fig. 1). Since the three groups reduce differently and the reduction of each produces a dif ...
... mixtures were analyzed in this work by Kern, the nature of the results suggests that the three disulfide bridges reduce at such different rates that it amounts to a specific reduction with little overlap (see Fig. 1). Since the three groups reduce differently and the reduction of each produces a dif ...
Geometrical and Sequence Characteristics of
... helix axes computed for every four consecutive Ca atoms. This window of four Ca atoms was slid over the length of the helix in steps of one Ca atom. The geometry of the helix is assigned from the values of four parameters, namely, the maximum local bending angle in the helix, defining the largest di ...
... helix axes computed for every four consecutive Ca atoms. This window of four Ca atoms was slid over the length of the helix in steps of one Ca atom. The geometry of the helix is assigned from the values of four parameters, namely, the maximum local bending angle in the helix, defining the largest di ...
Identification and removal of colanic acid from plasmid DNA
... including cGMP clinical grade preparations. CA is also required for bacterial growth in large-scale shake flasks or fermentors because it protects gram-negative bacteria from stress.20 Therefore, large-scale plasmid production in CA-mutant bacterial strains is not possible, and only small 5 ml cultu ...
... including cGMP clinical grade preparations. CA is also required for bacterial growth in large-scale shake flasks or fermentors because it protects gram-negative bacteria from stress.20 Therefore, large-scale plasmid production in CA-mutant bacterial strains is not possible, and only small 5 ml cultu ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... as extensively as those of pols a and p, but their response to PCNA has been investigated. PCNA can stimulate processive DNA synthesis for pol-6 on primed, single-stranded DNA primer template (Tan et al. 1986; Prelich et al. 1987; So and Downey 1988, 1992; Downey et al. 1990; Sabatino et al. 1990). ...
... as extensively as those of pols a and p, but their response to PCNA has been investigated. PCNA can stimulate processive DNA synthesis for pol-6 on primed, single-stranded DNA primer template (Tan et al. 1986; Prelich et al. 1987; So and Downey 1988, 1992; Downey et al. 1990; Sabatino et al. 1990). ...
attachment 1 - Federal Register of Legislation
... (iii) the composition and nutritional adequacy of the food, including whether there had been any unintended changes. No potential public health and safety concerns were identified. Therefore, on the basis of all the available evidence, including detailed studies provided by the Applicant, it has bee ...
... (iii) the composition and nutritional adequacy of the food, including whether there had been any unintended changes. No potential public health and safety concerns were identified. Therefore, on the basis of all the available evidence, including detailed studies provided by the Applicant, it has bee ...
Case Study: BPTI
... of a two dimensional plot of distances between the Cα atoms of any pair of amino acid residues in a protein can provide important and useful information regarding its the tertiary structure. Fig. 5 shows the contact map generated for BPTI, in which the pairwise Cα distances are displayed in a gray s ...
... of a two dimensional plot of distances between the Cα atoms of any pair of amino acid residues in a protein can provide important and useful information regarding its the tertiary structure. Fig. 5 shows the contact map generated for BPTI, in which the pairwise Cα distances are displayed in a gray s ...
A Metabolic Node in Action: Chorismate
... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
Glycemia and insulinemia in healthy subjects after
... low GI and a low II are both critical product characteristics for the observed metabolic benefits. As suggested by Augustin et al in 2002 (7), the link between a high-GI diet and diabetes may relate to elevated postprandial blood glucose peaks but also to an increased insulin demand. Insulin resista ...
... low GI and a low II are both critical product characteristics for the observed metabolic benefits. As suggested by Augustin et al in 2002 (7), the link between a high-GI diet and diabetes may relate to elevated postprandial blood glucose peaks but also to an increased insulin demand. Insulin resista ...
MORINGA OLEIFERA IN SILICO Research Article
... Receptor 2) and ERK (Extracellular Regulated Kinase) which usually require cPAP for their dephosphorylation and inactivation, were chosen as the drug targets. M. oleifera flower compounds were analyzed for their interactions with the drug targets using in silico methods and calculated for their suit ...
... Receptor 2) and ERK (Extracellular Regulated Kinase) which usually require cPAP for their dephosphorylation and inactivation, were chosen as the drug targets. M. oleifera flower compounds were analyzed for their interactions with the drug targets using in silico methods and calculated for their suit ...
Homology Modelling and Structural Comparisons of Capsid
... elude the host immune system, and can be related to the publication from Kundu et al. [21], showing that viruses infecting the same population may have diverse biological fitness due to genetic mutations that translate into slight amino acid variation. Specifically, Cap must successfully evade the h ...
... elude the host immune system, and can be related to the publication from Kundu et al. [21], showing that viruses infecting the same population may have diverse biological fitness due to genetic mutations that translate into slight amino acid variation. Specifically, Cap must successfully evade the h ...
Development of prokaryotic cell-free systems for synthetic
... bioRxiv preprint first posted online Apr. 15, 2016; doi: http://dx.doi.org/10.1101/048710. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ...
... bioRxiv preprint first posted online Apr. 15, 2016; doi: http://dx.doi.org/10.1101/048710. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ...
Multiple Lines of Evidence Localize Signaling
... cytosol to enter mitochondria, it also acts as a barrier for molecules to leave mitochondria. Thus, the outer mitochondrial membrane may also contain proteins involved in signal transduction and mediating retrograde signals from the mitochondrion to the nucleus or even from the mitochondrion to the ...
... cytosol to enter mitochondria, it also acts as a barrier for molecules to leave mitochondria. Thus, the outer mitochondrial membrane may also contain proteins involved in signal transduction and mediating retrograde signals from the mitochondrion to the nucleus or even from the mitochondrion to the ...
Novel Substrates for Fluorescence-based Protein Tyrosine Kinase
... Determination of kinase concentration – 0 to 30 nM of each kinase was incubated with 50 nM of biotinylated phospho-Tyr-peptide in the presence of 200 µM ATP for 2 h. The reaction was stopped by adding 10 mM EDTA and 50 nM ULight-Streptavidin for 5 min. 2 nM Europium-anti-phospho-Tyr (PT66) Antibody ...
... Determination of kinase concentration – 0 to 30 nM of each kinase was incubated with 50 nM of biotinylated phospho-Tyr-peptide in the presence of 200 µM ATP for 2 h. The reaction was stopped by adding 10 mM EDTA and 50 nM ULight-Streptavidin for 5 min. 2 nM Europium-anti-phospho-Tyr (PT66) Antibody ...
Insulin-like growth factor binding protein-2 (IGFBP
... amplification were performed using a cycle profile of 94 °C for 30 s, a primer-dependent annealing temperature (Table 1) for 30 s, 72 °C for 30 s. Based on the two IGFBP-2s partial fragment sequences, four pairs of gene-specific primers were designed, respectively, for 30 -end and 50 -end cloning. B ...
... amplification were performed using a cycle profile of 94 °C for 30 s, a primer-dependent annealing temperature (Table 1) for 30 s, 72 °C for 30 s. Based on the two IGFBP-2s partial fragment sequences, four pairs of gene-specific primers were designed, respectively, for 30 -end and 50 -end cloning. B ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.