Proteins of Human Milk. I. Identification of Major Components
... human milk (28). Casein micelles and soluble (whey) proteins are collected in to secretory vesicles fo r exocytosis (31). Microtubules appear to be involved in milk secretion, because milk prod uction is inhibited by colchi cine and vinblastine (26). T he composit ion of membranes that sedime nt wit ...
... human milk (28). Casein micelles and soluble (whey) proteins are collected in to secretory vesicles fo r exocytosis (31). Microtubules appear to be involved in milk secretion, because milk prod uction is inhibited by colchi cine and vinblastine (26). T he composit ion of membranes that sedime nt wit ...
University of Groningen Lactococcus lactis systems biology Eckhardt
... transcriptome 36, proteome and metabolome techniques and more recently, next generation sequencing (NGS) 37, the exciting possibility of describing a cell in its entirety is now a real option. Combining data from different–omics techniques is extremely powerful, since a more complete overview of cel ...
... transcriptome 36, proteome and metabolome techniques and more recently, next generation sequencing (NGS) 37, the exciting possibility of describing a cell in its entirety is now a real option. Combining data from different–omics techniques is extremely powerful, since a more complete overview of cel ...
Interactions of TCA cycle enzymes and of the CcpA
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
ribosome binding site Prokaryotic mRNAs have a ribosome binding
... the level of mRNA transcription, it is more effective for the cell to regulate gene expression at the level of translation. • As with other types of regulation, translational control typically functions at the level of initiation. ...
... the level of mRNA transcription, it is more effective for the cell to regulate gene expression at the level of translation. • As with other types of regulation, translational control typically functions at the level of initiation. ...
Kinetic mechanism of the dimeric ATP sulfurylase from plants
... genome contains four ATP sulfurylase genes (Glyma10g38760, Glyma20g28980, Glyma13g06940 and Glyma19g05020) encoding proteins with predicted plastidial and/or mitochondrial localization [4]. Biochemical analysis of a plastidic ATP sulfurylase from soybean [GmATPS1 (Glycine max (soybean) ATP sulfuryla ...
... genome contains four ATP sulfurylase genes (Glyma10g38760, Glyma20g28980, Glyma13g06940 and Glyma19g05020) encoding proteins with predicted plastidial and/or mitochondrial localization [4]. Biochemical analysis of a plastidic ATP sulfurylase from soybean [GmATPS1 (Glycine max (soybean) ATP sulfuryla ...
Pharos university Faculty of Allied Medical SCIENCE Biochemistry 1
... bonded to an amino group (-NH2), a carboxyl group (-COOH), a hydrogen atom, and a fourth group that differs from one amino acid to another and often is referred to as the-R group or the side chain. ...
... bonded to an amino group (-NH2), a carboxyl group (-COOH), a hydrogen atom, and a fourth group that differs from one amino acid to another and often is referred to as the-R group or the side chain. ...
A structural comparison of molybdenum cofactor
... the presence of their respective substrates, thereby allowing the bacteria to generate more energy compared to the amount obtainable by fermentation. DMSO reductase is found in a variety of bacteria, including E. coli [18], R. sphaeroides [19] and R. capsulatus [20]. DMSO reductase from R. sphaeroid ...
... the presence of their respective substrates, thereby allowing the bacteria to generate more energy compared to the amount obtainable by fermentation. DMSO reductase is found in a variety of bacteria, including E. coli [18], R. sphaeroides [19] and R. capsulatus [20]. DMSO reductase from R. sphaeroid ...
on the importance of cooperativity effects - RSC Publishing
... formation of complexes with uracil. Chloride shows the highest selectivity with all 13 anions interacting with the guanine ring, as can be also inferred from the comparison of the expected and actual amounts for the Cl–G pair. All these results are in stark contrast with the DNA results since in RNA ...
... formation of complexes with uracil. Chloride shows the highest selectivity with all 13 anions interacting with the guanine ring, as can be also inferred from the comparison of the expected and actual amounts for the Cl–G pair. All these results are in stark contrast with the DNA results since in RNA ...
emboj2009339-sup
... For analysis by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, 1 L of the fractionated, reconstituted peptides was mixed with an equal volume of an -cyano-4-hydroxycinnamic acid solution (10 mg/mL in 50% (v/v) ACN and 0.1% (v/v) TFA) supplemented with 10 ...
... For analysis by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, 1 L of the fractionated, reconstituted peptides was mixed with an equal volume of an -cyano-4-hydroxycinnamic acid solution (10 mg/mL in 50% (v/v) ACN and 0.1% (v/v) TFA) supplemented with 10 ...
A complete shikimate pathway in Toxoplasma gondii: an ancient
... 2.1) revealed two contigs (assembled genomic sequences) containing regions that appeared to code for a number of shikimate pathway enzymes. TGG 7014 contained sequences homologous to EPSP synthase, shikimate kinase, DHQase and shikimate dehydrogenase. The order of the elements on this contiguous reg ...
... 2.1) revealed two contigs (assembled genomic sequences) containing regions that appeared to code for a number of shikimate pathway enzymes. TGG 7014 contained sequences homologous to EPSP synthase, shikimate kinase, DHQase and shikimate dehydrogenase. The order of the elements on this contiguous reg ...
Energy „flow” in the organism
... Isodynamic law: for energy purposes different nutrients may replace one another in accordance with their caloric (energy) values Max Rubner – (1923) ...
... Isodynamic law: for energy purposes different nutrients may replace one another in accordance with their caloric (energy) values Max Rubner – (1923) ...
Sequence and phylogenetic analysis of the gp200 protein of
... A phylogenetic tree of E. canis was inferred based on the gp200 amino acid sequences obtained in this study and four other reported sequences [24]. In this tree, the gp200 sequence from the TWN genotype differed distinctly from other corresponding sequences of E. canis isolates from North America (t ...
... A phylogenetic tree of E. canis was inferred based on the gp200 amino acid sequences obtained in this study and four other reported sequences [24]. In this tree, the gp200 sequence from the TWN genotype differed distinctly from other corresponding sequences of E. canis isolates from North America (t ...
Annex 1
... 1. For the purposes of this standard the following definitions shall be applicable: (i) the expression "sequence listing" means a part of the description of the application as filed or a document filed subsequently to the application, which gives a detailed disclosure of the nucleotide and/or amino ...
... 1. For the purposes of this standard the following definitions shall be applicable: (i) the expression "sequence listing" means a part of the description of the application as filed or a document filed subsequently to the application, which gives a detailed disclosure of the nucleotide and/or amino ...
Biochemistry for the Radiation Biologist
... Acid-base catalysis (specific amino acid side-chains participate temporarily in reactions by acting as nucleophiles or electrophiles) Proximity effect (enzyme contains channels with appropriate shape and electrostatic properties to cause substrates to travel down them toward one another) Induced fit ...
... Acid-base catalysis (specific amino acid side-chains participate temporarily in reactions by acting as nucleophiles or electrophiles) Proximity effect (enzyme contains channels with appropriate shape and electrostatic properties to cause substrates to travel down them toward one another) Induced fit ...
Engineering the substrate and inhibitor specificities of human
... In order to identify the most optimal subsite for altering FVIIa specificity, we first evaluated the substrate specificity of FVIIa using PS-SCLs (positional scanning substrate combinatorial libraries). One aspect that such libraries do not always fully address is the potential interdependence betwe ...
... In order to identify the most optimal subsite for altering FVIIa specificity, we first evaluated the substrate specificity of FVIIa using PS-SCLs (positional scanning substrate combinatorial libraries). One aspect that such libraries do not always fully address is the potential interdependence betwe ...
The Old World monkey DAZ (Deleted in
... loci (AZFa, -b and -c). Microdeletions of AZFa–c could be detected in 5–15% of the infertile men, suggesting that the deleted regions of the Y chromosome contain genes essential for spermatogenesis (4,5). One candidate gene in the AZFc region is the recently described DAZ gene (Deleted in AZoospermi ...
... loci (AZFa, -b and -c). Microdeletions of AZFa–c could be detected in 5–15% of the infertile men, suggesting that the deleted regions of the Y chromosome contain genes essential for spermatogenesis (4,5). One candidate gene in the AZFc region is the recently described DAZ gene (Deleted in AZoospermi ...
Markers of renal function tests (PDF Available)
... The protease inhibitor Cystatin C is a non-glycosylated low molecular weight protein. Cystatin C has been proposed to be a marker as it is produced by all nucleated cells at a constant rate and is freely filtrated by the glomeruli and completely catabolized in the proximal tubules. The concentration ...
... The protease inhibitor Cystatin C is a non-glycosylated low molecular weight protein. Cystatin C has been proposed to be a marker as it is produced by all nucleated cells at a constant rate and is freely filtrated by the glomeruli and completely catabolized in the proximal tubules. The concentration ...
Amino Acids and Proteins - KSU Faculty Member websites
... The nonpolar R-groups thus fill up the interior of the folded protein and help give it its threedimensional shape. However, for proteins that are located in a hydrophobic environment, such as a membrane, the nonpolar R-groups are found on the outside surface of the protein, interacting with the lip ...
... The nonpolar R-groups thus fill up the interior of the folded protein and help give it its threedimensional shape. However, for proteins that are located in a hydrophobic environment, such as a membrane, the nonpolar R-groups are found on the outside surface of the protein, interacting with the lip ...
Intestinal peptidases form functional complexes with the neutral
... peptide hydrolases have been isolated from intestinal brush-border membranes using Blue native electrophoresis [19], but these did not seem to contain membrane transporters. There is also evidence of a role for intestinal microvillar micro-domains or lipid rafts in the sorting and trafficking of som ...
... peptide hydrolases have been isolated from intestinal brush-border membranes using Blue native electrophoresis [19], but these did not seem to contain membrane transporters. There is also evidence of a role for intestinal microvillar micro-domains or lipid rafts in the sorting and trafficking of som ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... Purification of3C proteinase. The HRV-14 3C proteinase and its derivative containing the cysteine (146) to serine mutation were produced directly in E. coli. The induction and growth of the E. coli cells expressing the respective plasmids and the details of the purification protocol were as describe ...
... Purification of3C proteinase. The HRV-14 3C proteinase and its derivative containing the cysteine (146) to serine mutation were produced directly in E. coli. The induction and growth of the E. coli cells expressing the respective plasmids and the details of the purification protocol were as describe ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... candidate, because its side chain is voluminous and projects into the putative cavity where the acyl-CoA may enter. Our bioinformatic approach failed to identify Met564; in our protein alignment we compared all the known sequences of the carnitine/choline acyltransferase family from several organism ...
... candidate, because its side chain is voluminous and projects into the putative cavity where the acyl-CoA may enter. Our bioinformatic approach failed to identify Met564; in our protein alignment we compared all the known sequences of the carnitine/choline acyltransferase family from several organism ...
Gq-dependent signalling by the lysophosphatidic acid receptor
... muscle. Expression of LPA3 receptors, but not LPA1 and LPA2 , receptors was demonstrated by Western blot analysis. LPA stimulated phosphoinositide hydrolysis, PKC (protein kinase C) and Rho kinase (Rho-associated kinase) activities: stimulation of all three enzymes was inhibited by expression of the ...
... muscle. Expression of LPA3 receptors, but not LPA1 and LPA2 , receptors was demonstrated by Western blot analysis. LPA stimulated phosphoinositide hydrolysis, PKC (protein kinase C) and Rho kinase (Rho-associated kinase) activities: stimulation of all three enzymes was inhibited by expression of the ...
Intestinal Protein digestion in vitro Assay
... Antoniewicz, A. M., Van Vuuren, A. M., van der Koelen, C. J., and Kosmala, I. 1992. Intestinal digestibility of rumen undegraded protein of formaldehyde-treated feedstuffs measured by mobile bag and in vitro technique. An Feed Sci Tech 39, 111-124. Ben-Ghedalia, D., Tagari, H., and Bondi, A. 1974. P ...
... Antoniewicz, A. M., Van Vuuren, A. M., van der Koelen, C. J., and Kosmala, I. 1992. Intestinal digestibility of rumen undegraded protein of formaldehyde-treated feedstuffs measured by mobile bag and in vitro technique. An Feed Sci Tech 39, 111-124. Ben-Ghedalia, D., Tagari, H., and Bondi, A. 1974. P ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.