Regulation of thiamine synthesis in Saccharomyces cerevisiae for
... THI3, were disrupted in the S. cerevisiae parent strain FMME-002. The mutants FMME-002ΔTHI2 and FMME-002ΔTHI3 both exhibited an enhanced pyruvate yield. Moreover, FMME-002ΔTHI2 achieved a relatively higher pyruvate production, and the highest concentration of pyruvate was achieved when 0.04 mM thiam ...
... THI3, were disrupted in the S. cerevisiae parent strain FMME-002. The mutants FMME-002ΔTHI2 and FMME-002ΔTHI3 both exhibited an enhanced pyruvate yield. Moreover, FMME-002ΔTHI2 achieved a relatively higher pyruvate production, and the highest concentration of pyruvate was achieved when 0.04 mM thiam ...
Novel Allergen Structures with Tandem Amino Acid Repeats
... The Bla g 1 and Per a 1 Allergens Are Composed of Multiple Amino Acid Repeats—A unique feature of the primary structure of Bla g 1.01 was the existence of multiple amino acid repeats, which have not previously been found in allergen sequences. Close inspection of the sequences revealed segments that ...
... The Bla g 1 and Per a 1 Allergens Are Composed of Multiple Amino Acid Repeats—A unique feature of the primary structure of Bla g 1.01 was the existence of multiple amino acid repeats, which have not previously been found in allergen sequences. Close inspection of the sequences revealed segments that ...
NPTEL-Module-2: Bioorganic Chemistry of Amino Acids Dr. S. S.
... Structural chemistry, Bioinformatics, Proteomics, Organic chemistry, Pharmacology, Medicinal chemistry and many others. The field is primarily concerned with research at the interface of Chemistry and Biology. But in practical sense it is largely focused on small molecules as research tools and pote ...
... Structural chemistry, Bioinformatics, Proteomics, Organic chemistry, Pharmacology, Medicinal chemistry and many others. The field is primarily concerned with research at the interface of Chemistry and Biology. But in practical sense it is largely focused on small molecules as research tools and pote ...
phylogenetic analysis of the rompb genes of rickettsia felis and
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
Intraplastidic Localization of the Enzymesthat Convert Cucumber
... green pea leave chloroplasts, Smith (26) reached similar conclusions upon investigating the intracellular distribution of PBGD and ALA dehydratase (EC 4.2.1.24). The latter converts two molecules of ALA to PBG. Earlier work, however, by Carell and Kahn (3) had indicated that in Euglena chloroplasts ...
... green pea leave chloroplasts, Smith (26) reached similar conclusions upon investigating the intracellular distribution of PBGD and ALA dehydratase (EC 4.2.1.24). The latter converts two molecules of ALA to PBG. Earlier work, however, by Carell and Kahn (3) had indicated that in Euglena chloroplasts ...
Characterization Of A 36 Kda Fish Protein And Its
... The overall goal of this study was, therefore, to develop a sandwich enzyme linked immunosorbent assay (sELISA) for the detection of fish and fish products. The specific objectives were to: 1) identify a suitable marker protein to indicate the presence of fish in food; and 2) develop an immunoassay ...
... The overall goal of this study was, therefore, to develop a sandwich enzyme linked immunosorbent assay (sELISA) for the detection of fish and fish products. The specific objectives were to: 1) identify a suitable marker protein to indicate the presence of fish in food; and 2) develop an immunoassay ...
Characterization of the cDNA and Gene Coding for the Biotin
... pBS-1 was confirmed upon sequencing of the corresponding gene (see below). At the 3' end, the pBS-1 sequence contains an extra 58 and 19 nucleotides upstream of the poly(A) tail that are not present in the sequence shown in GenBank accession number L34413 or in the sequence of Patton et al., respect ...
... pBS-1 was confirmed upon sequencing of the corresponding gene (see below). At the 3' end, the pBS-1 sequence contains an extra 58 and 19 nucleotides upstream of the poly(A) tail that are not present in the sequence shown in GenBank accession number L34413 or in the sequence of Patton et al., respect ...
METABOLIC AND ENDOCRINE ADAPTATIONS TO HEAT STRESS
... negatively correlated to milk yield and feed intake. In addition, high producers generate more metabolic heat than non-lactating or low producers because of their metabolic processes associated with production and elevated digestive requirements (West, 2003). Therefore, high producers have a lower t ...
... negatively correlated to milk yield and feed intake. In addition, high producers generate more metabolic heat than non-lactating or low producers because of their metabolic processes associated with production and elevated digestive requirements (West, 2003). Therefore, high producers have a lower t ...
Supplemental Table 3
... 0,000003059249 Elemental activities, such as catalysis or binding, describing the actions of a gene product at the molecular level. A given gene product may exhibit one or more molecular functions [goid 3674] [evidence ND ]; Interacting selectively with DNA (deoxyribonucleic acid) [goid 3677] [evide ...
... 0,000003059249 Elemental activities, such as catalysis or binding, describing the actions of a gene product at the molecular level. A given gene product may exhibit one or more molecular functions [goid 3674] [evidence ND ]; Interacting selectively with DNA (deoxyribonucleic acid) [goid 3677] [evide ...
Autism One
... It modulates the activity of membrane - bound enzymes, ion channels and receptors. The activity of integral membrane proteins are markedly affected by the physical state of the lipids in which they are embedded. ...
... It modulates the activity of membrane - bound enzymes, ion channels and receptors. The activity of integral membrane proteins are markedly affected by the physical state of the lipids in which they are embedded. ...
Amendment Determination 2007
... to cows' milk protein and protein hydrolysate formulae in a child aged less than 2 years, where combined intolerance is demonstrated when the child has failed to respond to a strict cows' milk protein free diet with a protein hydrolysate (with or without medium chain triglycerides) as the principal ...
... to cows' milk protein and protein hydrolysate formulae in a child aged less than 2 years, where combined intolerance is demonstrated when the child has failed to respond to a strict cows' milk protein free diet with a protein hydrolysate (with or without medium chain triglycerides) as the principal ...
Characters of Chymosin Gene Isolated from Different Animal A. G. Attallah
... for camel, pig, buffalo and cow respectively and it was corresponding with the sequence obtained from genomic analysis of pre-prochymosin gene [1 4 ]. Fig. (3) shows that PCR profiles of each primer we found that two main bands of approximately 1.2 kb and 0.25 kb for buffalo chymosin, 1.1 kb and 0.2 ...
... for camel, pig, buffalo and cow respectively and it was corresponding with the sequence obtained from genomic analysis of pre-prochymosin gene [1 4 ]. Fig. (3) shows that PCR profiles of each primer we found that two main bands of approximately 1.2 kb and 0.25 kb for buffalo chymosin, 1.1 kb and 0.2 ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... This application is a continuation-in-part of Ser. No. 09/337,827, ?led Jun. 22, 1999 noW abandoned; Which is a divisional of Ser. No. 08/953,326, ?led Oct. 17, 1997, now US. Pat. No. 6,251,872; Which is a continuation-in-part of ...
... This application is a continuation-in-part of Ser. No. 09/337,827, ?led Jun. 22, 1999 noW abandoned; Which is a divisional of Ser. No. 08/953,326, ?led Oct. 17, 1997, now US. Pat. No. 6,251,872; Which is a continuation-in-part of ...
BACILLUS SPHAERICUS TOXINS: Molecular Biology and Mode of
... contain active strains. Such as numerical classification based on taxonomy of phenotypic features (2, 45), cellular fatty acid composition analysis (43) ribotyping (3) and random amplified polymorphic DNA analysis (80)are recently developed techniques, indicate most toxic strains are recovered in a ...
... contain active strains. Such as numerical classification based on taxonomy of phenotypic features (2, 45), cellular fatty acid composition analysis (43) ribotyping (3) and random amplified polymorphic DNA analysis (80)are recently developed techniques, indicate most toxic strains are recovered in a ...
CO2 and O2 Distribution in Rubisco Suggests the
... carbamylated Lys201 is stabilized by the binding of a magnesium ion to the carbamate group. During catalysis, Rubisco undergoes a conformational change, which closes the active site and prevents access of solvent during the reaction. The transition between open and closed forms involves rigid-body d ...
... carbamylated Lys201 is stabilized by the binding of a magnesium ion to the carbamate group. During catalysis, Rubisco undergoes a conformational change, which closes the active site and prevents access of solvent during the reaction. The transition between open and closed forms involves rigid-body d ...
Crosslinking Technical Handbook
... what is crosslinking? Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups ...
... what is crosslinking? Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. The availability of several chemical groups ...
12659942_three sites - University of Canterbury
... backbone carbonyl of Phe91, the side-chain carbonyl of Asn175 and, mediated through a water molecule, the main-chain carbonyl group of Asn94. The two Tyr ligands in different subunits are relatively close to each other, with the distance between their hydroxyl groups being 4.8 Å. The binding mode of ...
... backbone carbonyl of Phe91, the side-chain carbonyl of Asn175 and, mediated through a water molecule, the main-chain carbonyl group of Asn94. The two Tyr ligands in different subunits are relatively close to each other, with the distance between their hydroxyl groups being 4.8 Å. The binding mode of ...
to the PDF file. - CURVE
... treatment of trichomoniasis, an infection of the genito-urinary tract of humans by the protozoan Trichomonas vaginalis, has created a need for the development of therapeutics with a different mode of action. Trifluoromethionine (TFM) has been proposed as a potential novel antiinfective prodrug that ...
... treatment of trichomoniasis, an infection of the genito-urinary tract of humans by the protozoan Trichomonas vaginalis, has created a need for the development of therapeutics with a different mode of action. Trifluoromethionine (TFM) has been proposed as a potential novel antiinfective prodrug that ...
Communication
... subsequent procedures were carried out at 4 °C. Tissue powder was collected into 50-ml sterile conical tubes, Buffer C was added (5 ml/g tissue), and the tubes were rocked gently overnight. Tissue was removed by centrifugation at 5000 3 g for 15 min, the supernatant was subjected to centrifugation a ...
... subsequent procedures were carried out at 4 °C. Tissue powder was collected into 50-ml sterile conical tubes, Buffer C was added (5 ml/g tissue), and the tubes were rocked gently overnight. Tissue was removed by centrifugation at 5000 3 g for 15 min, the supernatant was subjected to centrifugation a ...
02_whole - Massey Research Online
... restriction sites are unique to each individual MD-AeO sequence. Genomic Southern analysis, using probes spanning the 3 '-UTR and the 3 '-end of the coding region confirmed that MD A e03 is encoded by a distinct gene. However, while the distinction between MD-A eO] and MD-A e02 is not as definitive ...
... restriction sites are unique to each individual MD-AeO sequence. Genomic Southern analysis, using probes spanning the 3 '-UTR and the 3 '-end of the coding region confirmed that MD A e03 is encoded by a distinct gene. However, while the distinction between MD-A eO] and MD-A e02 is not as definitive ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... restriction enzymes and cloned into pHPO11172 plasmid vector pretreated with the same restriction enzymes. After transforming E. coli JM109 strain with the library, the transformants were cultivated on LB plates containing ampicillin as antibiotics to construct the HPO cassette PCR metagenomic libra ...
... restriction enzymes and cloned into pHPO11172 plasmid vector pretreated with the same restriction enzymes. After transforming E. coli JM109 strain with the library, the transformants were cultivated on LB plates containing ampicillin as antibiotics to construct the HPO cassette PCR metagenomic libra ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.