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The Plant Cell
The Plant Cell

Glycolic acid production in the engineered yeasts Saccharomyces
Glycolic acid production in the engineered yeasts Saccharomyces

... knowledge. With E. coli, the fermentation conditions are limited due to their need to be maintained close to a neutral pH. The benefit with many fungal species is their tolerance towards low pH conditions, which is advantageous as the decreased need for neutralisation reduces production costs and th ...
Glutathione as an endogenous sulphur source in the
Glutathione as an endogenous sulphur source in the

... Buthionine-(S,R)-sulphoximine(BSO), a specific transition-state inhibitor of y-glutamylcysteine synthetase (Griffith & Meister, 1979),lowered the GSH level in cells of S. cerevisiae (Fig. 2). Depletion with BSO enabled us to check the effect of GSH on y-GT biosynthesis in the wild-type strain 1278b. ...
Abstract Background The present study aimed to compare the
Abstract Background The present study aimed to compare the

Gene Section MUC4 (mucin 4, cell surface associated) in Oncology and Haematology
Gene Section MUC4 (mucin 4, cell surface associated) in Oncology and Haematology

... amino acids repeated in tandem up to 400 times. This repetitive domain is the hallmark of the mucin family. The carboxy-extremity of MUC4a harbors a NIDO and an AMOP domain. The NIDO domain is a domain identified in the nidogen protein and present in several proteins such as the tumor endothelial ma ...
CD-HIT User`s Guide - Bioinformatics.org
CD-HIT User`s Guide - Bioinformatics.org

... A   limitation   of   short   word   filter   is   that   it   can   not   be   used   below   certain   clustering   thresholds.   In   a   worst   case   scenario   (figure   below),   when   mismatches   are   evenly   distributed   along ...
Recent Developments in Patent Law
Recent Developments in Patent Law

... encoded protein is known; and – General method employing a probe specifically corresponding to the known amino acid sequence for the protein. – Discloses that it is advantageous to design the probe based on amino acids specified by unique codons. ...
Genetic Engineering for Improved Xylose Fermentation by Yeasts
Genetic Engineering for Improved Xylose Fermentation by Yeasts

... glucose, but glucose also represses the utilization of xylose, so fermentation of glucose and xylose in mixtures presents a complex challenge. Because of the rarity of xylose-fermenting yeasts, it is appropriate to ask what makes them special. How do they take up xylose, make ethanol, respire, and c ...
711 BOMB CARBON AS A TRACER OF DIETARY CARBON
711 BOMB CARBON AS A TRACER OF DIETARY CARBON

... implicated as a predator on chicks and eggs of seabirds and some terrestrial birds (Craig 1986; Booth et al. 1996). Both its short lifespan and omnivorous diet make R. exulans an appropriate animal to study diet using ∆14C variations. We use the “bomb pulse” 14C to provide supplemental information o ...
Phosphorylation of the F1Fo ATP Synthase Я Subunit
Phosphorylation of the F1Fo ATP Synthase Я Subunit

... Rationale: We previously discovered several phosphorylations to the ␤ subunit of the mitochondrial F1Fo ATP synthase complex in isolated rabbit myocytes on adenosine treatment, an agent that induces cardioprotection. The role of these phosphorylations is unknown. Objective: The present study focuses ...
Effect of Alanine-293 Replacement on the Activity, ATP Binding, and
Effect of Alanine-293 Replacement on the Activity, ATP Binding, and

SODIUM-COUPLED TRANSPORTERS FOR KREBS CYCLE
SODIUM-COUPLED TRANSPORTERS FOR KREBS CYCLE

... rat kidney. The Na+/dicarboxylate cotransporters are part of a distinct gene family that includes the renal and intestinal Na+/sulfate cotransporters. Other members of this family include a Na+- and Li+-dependent dicarboxylate transporter from Xenopus intestine and a putative Na+/dicarboxylate cotra ...
Protein Structure, Neighbor Effect, and a New Index of Amino Acid
Protein Structure, Neighbor Effect, and a New Index of Amino Acid

... Sasisekharan 1968) and subsequent empirical studies (Morris et al. 1992), only particular combinations of these two angles can give rise to, or maintain, the basic secondary structures such as a-helices or b-sheets. In other words, only particular combinations of amino acids can cooperate to form pa ...
Nutrient Sensing through the Plasma Membrane of Eukaryotic Cells
Nutrient Sensing through the Plasma Membrane of Eukaryotic Cells

... Furthermore, feedback and feedforward regulatory loops govern the expression of genes involved in the Snf3/Rgt2 glucose sensing pathway. We can recognize three such controls (shown in Figure 3) [45]. (1) The glucose induction pathway regulates itself through Rgt1-mediated glucose induction of STD1 e ...
Oral free and dipeptide forms of glutamine supplementation
Oral free and dipeptide forms of glutamine supplementation

Plant Biochemistry
Plant Biochemistry

... This textbook is written for students and is the product of more than three decades of my teaching experience. It intends to give a broad but concise overview of the various aspects of plant biochemistry, including molecular biology. I have attached importance to an easily understood description of ...
H. Heldt
H. Heldt

... This textbook is written for students and is the product of more than three decades of my teaching experience. It intends to give a broad but concise overview of the various aspects of plant biochemistry, including molecular biology. I have attached importance to an easily understood description of ...
Insulin hormone: Mechanism and effects on the body and
Insulin hormone: Mechanism and effects on the body and

... 2 domains. IRS-1 in particular is phosphorylated on several tyrosine residues by the insulin receptor’s intracellular catalytic part and these phosphorylated tyrosine residues in turn employee other proteins with src homology 2 domains, one of which is phosphatidylinositol 3-kinase. Phosphatidylinos ...
Fermentation of lignocellulosic material
Fermentation of lignocellulosic material

... Lignocellulosic media is a harsh substrate to ferment, partly due to the lignin degradation products as well as the presence of acids (Palmqvist and Hanh-Hägerdal, 2000b). Lignocellulosics are a diverse group of substrates and the amount of inhibitors is highly dependent on the origin of the materia ...
Molecular basis of hepatic carnitine palmitoyltransferase I deficiency
Molecular basis of hepatic carnitine palmitoyltransferase I deficiency

... both parents (Fig. 1) indicates that the patient is truly homozygous for this mutation. Expression studies. The aspartate at position 454 is located in a region which is highly conserved among 17 reported carnitine acyltransferases (Fig. 2). To investigate the effect of the mutation on the enzyme ac ...
The Plasma Membrane - Beck-Shop
The Plasma Membrane - Beck-Shop

... The plasma membrane accounts for about 10% to 25% of cell dry weight. In some instances lipoproteins extend outward from the plasma membrane and consist of carbohydrate polymers that are covalently linked to protein in the plasma membrane. The membrane is not rigid with the lipids covalently linked ...
Japanese Journal of Ophthalmology Vol.43 No.4
Japanese Journal of Ophthalmology Vol.43 No.4

... prolonged use of corticosteroids. Thus, agents with 0021-5155/99/$–see front matter PII S0021-5155(99)00027-1 ...
Module 3
Module 3

... 64. Who synthesized uric acid artificially from glycine and urea in 1882: A. German scientist Veller B. German scientist Fisher C. Polish-Russian scientist Nenskiy D. Austrian physiologist Maresh E. * Ukrainian scientist I. Horbachevskiy 65. Why constant excessive consumption of meat and glandular t ...
Lessons from the Genome Sequence of Neurospora crassa: Tracing
Lessons from the Genome Sequence of Neurospora crassa: Tracing

... University, Columbus, Ohio 4321025; The Institute for Genomic Research, Rockville, Maryland 2087826; and Department of Molecular Biology and Biochemistry, University of California, Irvine, California 9269727 INTRODUCTION ............................................................................... ...
3 Amino Acids - Minificciones
3 Amino Acids - Minificciones

... We now turn to amino acids having positively charged side chains that render these amino acids highly hydrophilic (Figure 3.5). Lysine and arginine have relatively long side chains that terminate with groups that are positively charged at neutral pH. Lysine is topped by an amino group and arginine b ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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