• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Mitochondria - Physical Mathematics
Mitochondria - Physical Mathematics

... most striking features of both mitochondria and chloroplasts are their extensive internal membrane systems. These internal membranes contain sets of membrane protein complexes that work together to produce most of the cell’s ATP. In bacteria, simpler versions of essentially the same protein complexe ...
Patent constraints
Patent constraints

... 968-986; 989-993; 995-1010; 1012-1034; 1043-1063; 1074-1080; 1091-1104; 1111-1121; 1123-1128; 11341138; 1142-1156; 1159-1175; 1187-1190; 1192-1199; 1202-1220; 1249-1253; 1258-1262; 1264-1269; 12711287; 1292-1301; 1303-1309; 1315-1323; 1328-1337; 1340-1341; 1344-1361; 1365-1377; 1379-1390; 13931394; ...
Hücrelerin Yapısı - mustafaaltinisik.org.uk
Hücrelerin Yapısı - mustafaaltinisik.org.uk

... membrane by denaturing the membrane (organic solvents, or strong detergents) • Often transmembrane but not necessarily • Glycophorin, bacteriorhodopsin are examples Copyright © 1999 by Harcourt Brace & Company ...
PKU
PKU

... Clinics used to think that after age 6 phe would not hurt your brain. Now we know differently. ...
Multiple Functions of the New Cytokine
Multiple Functions of the New Cytokine

... two transcription and translation variants: a long form (lfTSLP) that is induced by inflammation, and a short, constitutively-expressed form (sfTSLP), that appears to be downregulated by inflammation. The TSLP forms can be produced by a number of cell types, including epithelial and dendritic cells ...
Characterization of the Sucrose Phosphate Phosphatase (SPP
Characterization of the Sucrose Phosphate Phosphatase (SPP

... catalysed by SPS in the direction of sucrose synthesis [9, 10]. SPP encoding genes have been described in different plant species such as Arabidopsis, tomato, rice, wheat, maize and coffee [11, 12] where they constitute gene families with different number of members depending on the species. However ...
The Genera Staphylococcus and Macrococcus
The Genera Staphylococcus and Macrococcus

... with the typical appearance of S. aureus. If several types of colonies appear to be S. aureus, count the number of colonies of each type and record counts separately. When plates at the lowest dilution plated contain < 20 colonies, they may be used. If plates containing > 200 colonies have colonies ...
5 Molecular basis of type-2 diabetes
5 Molecular basis of type-2 diabetes

Bacterial methionine biosynthesis
Bacterial methionine biosynthesis

... Methionine is essential in all organisms, as it is both a proteinogenic amino acid and a component of the cofactor, S-adenosyl methionine. The metabolic pathway for its biosynthesis has been extensively characterized in Escherichia coli; however, it is becoming apparent that most bacterial species d ...
NIH Public Access
NIH Public Access

... STACs activate SIRT1 in vitro by lowering its peptide Michaelis constant (KM) and produce pharmacological changes consistent with SIRT1 activation (4–7). However, the legitimacy of STACs as direct SIRT1 activators has been widely debated. In previous studies, STACs increased SIRT1 activity toward fl ...
The semi-phosphorylative Entner–Doudoroff pathway in
The semi-phosphorylative Entner–Doudoroff pathway in

... The phosphorylation of KDG by ATP was monitored by coupling the formation of KDPG with the reduction of NAD+ via KD(P)G aldolase and GAPN of T. tenax [32]. The standard assay was performed in 100 mM Hepes/KOH (pH 7.0, 70 ◦C) in the presence of KDG kinase (1.5 and 3 µg of T. tenax protein after gel f ...
Answers to Quiz Questions
Answers to Quiz Questions

... All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, 999 Riverview Drive, Suite 208, Totowa, NJ 07512 USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with ...
Cyclic Adenosine Monophosphate Signaling Pathway
Cyclic Adenosine Monophosphate Signaling Pathway

Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using
Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using

... polysaccharea, Deinococcus radiodurans, Deinococcus geothermalis, Alteromonas macleodii, and Arthrobacter chlorophenolicus, have been studied [4, 13–16]. A previous study reported that the suh gene from Xanthomonas axonopodis pv. glycines, a causative agent of bacterial pustule disease in soybeans, ...
EFFECTS OF INSULIN DEFICIENCY ON EXERCISE
EFFECTS OF INSULIN DEFICIENCY ON EXERCISE

... the main nutrients. Its main function is to stimulate glucose uptake and disposal or utilization by the cells and thus to decrease blood glucose concentration. However, it also inhibits breakdown of proteins and lipids and promotes their synthesis. Type 1 diabetes is a disease in which insulin secre ...
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose

... well known that Rubisco content changes drastically during leaf development; for instance, it rapidly increases during leaf expansion and then decreases during the initial stages of leaf senescence in rice and cucumber (Makino et al. 1984, Yamauchi et al. 2002). However, the degradation mechanism of ...
Chapter 9 Slides
Chapter 9 Slides

... membrane by denaturing the membrane (organic solvents, or strong detergents) • Often transmembrane but not necessarily • Glycophorin, bacteriorhodopsin are examples Copyright © 1999 by Harcourt Brace & Company ...
Structure and mechanism of inhibition of plant
Structure and mechanism of inhibition of plant

... inhibition is time-dependent. Activity will be regenerated, at least partially, if conditions allow replacement of the damaged cofactor with fresh ThDP. Damage to ThDP in AHAS, even in the absence of herbicides, has been demonstrated directly [39] and this damage occurs more rapidly during turnover. ...
The mitochondrial carnitine/acylcarnitine carrier: Function
The mitochondrial carnitine/acylcarnitine carrier: Function

... Oxidation of fatty acids in mitochondria coupled to oxidative phosphorylation is the most important pathway for the production of metabolic energy during fasting. This process occurs in the mitochondrial matrix where the enzymes of fatty acid b-oxidation are located. Fatty acyl groups are transporte ...
Sequence and Structure Classification of Kinases
Sequence and Structure Classification of Kinases

... allows for the inference of biochemical and biological functional properties. Structure analysis methods are able to detect evolutionary relationships that sequence similarity searches miss, because protein structure conservation persists after sequence similarity disappears. However, similarity of ...
Chapter 2 Immobilization of Enzymes
Chapter 2 Immobilization of Enzymes

... Enzymes have the ability to catalyze reactions under very mild conditions with a very high degree of substrate specificity, thus decreasing the formation of by-products. Among the reactions catalyzed are a number of very complex chemical transformations between biological macromolecules, which are n ...
post- translational modifications
post- translational modifications

... Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs considerably increase the diversity of protein str ...
Fibrous Proteins
Fibrous Proteins

... peptidase)-In most cases, the step of collagen synthesis which is disturbed in Ehlers-Danlos is the formation of cross-links  Mutation in the amino acid sequence of Collagen I,III or V  The replacement of glycine with another amino acid may lead to EDS  Glycine is used because it has the smallest ...
Modeling Multi-typed Structurally Viewed Chemicals with the UMLS
Modeling Multi-typed Structurally Viewed Chemicals with the UMLS

... because some of the original structural components are expended in its creation. The neutralization reaction of an acid and a base producing a salt is a simple example of this scenario. The new chemical, salt, contains parts of acid and base; however, it is neither an acid nor a base. In this sense, ...
A Truncated Laminin Chain Homologous to the B2 Chain: Structure
A Truncated Laminin Chain Homologous to the B2 Chain: Structure

... chains, which would not crosshybridize, fragments from the least homologous 3' end region of the cDNAs were used. For the laminin B2t chain a PstI-EcoRI fragment of clone L15 (bases 2995-3840, Fig. D and for the laminin B2e chain a polymerase chain reaction (PCR) fragment with artificial BamHI-PstI ...
< 1 ... 5 6 7 8 9 10 11 12 13 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report