... epithelial cell CaM kinase by increasing intracellular Ca2+via To generate a mutant of y~ CaM kinase in which Thr**' is replaced with Asp (T287D),site directed mutagenesis was carried out as adenosine receptors is being pursued as a therapeutic apelsewhere (17, 21) using single-stranded DNA prepared ...
... The present invention relates to nucleic acid molecules related to the var2csa gene family as well as amino acid sequences encoded by such nucleic acid molecules with respect to their role in mediating adhesion of infected red blood cells to chondroitin sulphate A (CSA) in the placenta which is char ...
Specific features of glycogen metabolism in the liver
... found in io. This paradoxical finding led them to invoke the existence of a still-unidentified stimulator of glycogen synthase or of an alternative pathway for glycogen synthesis. However, it is questionable whether the adopted effector concentrations are physiologically relevant, in particular in ...
... found in io. This paradoxical finding led them to invoke the existence of a still-unidentified stimulator of glycogen synthase or of an alternative pathway for glycogen synthesis. However, it is questionable whether the adopted effector concentrations are physiologically relevant, in particular in ...
PDF Full-text
... the microelectrode when the adsorbent consisted of PS microbeads with a SPG-modified surface. A different protein immobilization method involves using a protein implanted with a ligand that acts as a tag [9]. The tag consisted of a continuous sequence of seven to eight histidine residues and could b ...
... the microelectrode when the adsorbent consisted of PS microbeads with a SPG-modified surface. A different protein immobilization method involves using a protein implanted with a ligand that acts as a tag [9]. The tag consisted of a continuous sequence of seven to eight histidine residues and could b ...
Calreticulin, a multi-process calcium
... 30 % of all cellular proteins that are synthesized into the ER, where they interact with molecular chaperones and are transported as cargo through the ER to different intracellular destinations as well as to the extracellular environment. Within the ER, nascent unfolded proteins interact with molecu ...
... 30 % of all cellular proteins that are synthesized into the ER, where they interact with molecular chaperones and are transported as cargo through the ER to different intracellular destinations as well as to the extracellular environment. Within the ER, nascent unfolded proteins interact with molecu ...
The Bacterial Toxin RelE Displays Codon
... not in the absence (Lane 5) of RelE and that the presence of RelB in excess over RelE completely inhibited the reaction (Lane 4). When the RC had been preincubated with RF1, which binds very stably to the ribosomal A site (Zavialov et al., 2002), there was little cleavage of the mRNA by RelE (Lane 8 ...
... not in the absence (Lane 5) of RelE and that the presence of RelB in excess over RelE completely inhibited the reaction (Lane 4). When the RC had been preincubated with RF1, which binds very stably to the ribosomal A site (Zavialov et al., 2002), there was little cleavage of the mRNA by RelE (Lane 8 ...
Structure of the Actin-Myosin Complex and Its Implications for
... itself (3, 4). The contractile cycle deduced from kinetic studies has shown that Mg2`ATP rapidly dissociates the actomyosin complex by binding to the ATPase active site of myosin; free myosin then hydrolyzes ATP and forms a stable myosin-products complex; actin recombines with this complex and disso ...
... itself (3, 4). The contractile cycle deduced from kinetic studies has shown that Mg2`ATP rapidly dissociates the actomyosin complex by binding to the ATPase active site of myosin; free myosin then hydrolyzes ATP and forms a stable myosin-products complex; actin recombines with this complex and disso ...
Short-term regulation of the mammalian pyruvate dehydrogenase
... PDHK1 (Patel & Korotchkina, 2003). In the case of site 3, the differences in the activity of the PDHK isoforms are not so clear. According to Kolobova et al. (2001), PDHK1 can phosphorylate all three sites, whereas PDHK2, PDHK3 and PDHK4 each phosphorylates only sites 1 and 3. Earlier, it was reporte ...
... PDHK1 (Patel & Korotchkina, 2003). In the case of site 3, the differences in the activity of the PDHK isoforms are not so clear. According to Kolobova et al. (2001), PDHK1 can phosphorylate all three sites, whereas PDHK2, PDHK3 and PDHK4 each phosphorylates only sites 1 and 3. Earlier, it was reporte ...
patrick_tb_ch18b
... example, resistance to paclitaxel can arise due to mutation of tubulin resulting in a weakened binding interaction with paclitaxel. Resistance can also arise due to overexpression of P-glycoprotein. This molecule is capable of transporting a variety of drugs across the cell membrane and out of the c ...
... example, resistance to paclitaxel can arise due to mutation of tubulin resulting in a weakened binding interaction with paclitaxel. Resistance can also arise due to overexpression of P-glycoprotein. This molecule is capable of transporting a variety of drugs across the cell membrane and out of the c ...
Function and Control of the Spx-Family of Proteins Within
... with yjbC that is controlled by several minor forms of RNA polymerase σ subunit, including σM, which was observed to stimulate yjbCspx transcription (Jervis et al. 2007; Thackray and Moir 2003). An investigation into the role of the ATPase, ClpX in sporulation and competence gene expression uncovere ...
... with yjbC that is controlled by several minor forms of RNA polymerase σ subunit, including σM, which was observed to stimulate yjbCspx transcription (Jervis et al. 2007; Thackray and Moir 2003). An investigation into the role of the ATPase, ClpX in sporulation and competence gene expression uncovere ...
Involvement of Polyamine Catabolism in the Regulation of Glucose
... Polyamines (putrescine, spermidine and spermine) are ubiquitous low molecular weight amines that are positively charged under physiological conditions. Homeostatic control of intracellular polyamine levels is achieved by regulating the synthesis, catabolism and transport of these molecules. Spermidi ...
... Polyamines (putrescine, spermidine and spermine) are ubiquitous low molecular weight amines that are positively charged under physiological conditions. Homeostatic control of intracellular polyamine levels is achieved by regulating the synthesis, catabolism and transport of these molecules. Spermidi ...
Value-added Conversion of Waste Cooking Oil, Post
... and a cost analysis for the conversion of WCO and waste PET into biodiesel and PU foams indicate potential economic viability of the developed process. Soybean meal (SM) is the soy protein residue after the extraction of oil from soybeans. With its high protein content, SM has the potential to becom ...
... and a cost analysis for the conversion of WCO and waste PET into biodiesel and PU foams indicate potential economic viability of the developed process. Soybean meal (SM) is the soy protein residue after the extraction of oil from soybeans. With its high protein content, SM has the potential to becom ...
Patterns, motifs, PSI-BLAST
... • One possible trick is to add a small number to all observed frequencies. These small non-observed frequencies are referred to as pseudo-counts. • From the previous example with a pseudo-counts of 1: ...
... • One possible trick is to add a small number to all observed frequencies. These small non-observed frequencies are referred to as pseudo-counts. • From the previous example with a pseudo-counts of 1: ...
Relació estructura-funció en la família de transportadors d'aminoàcids heteromultimèrics.
... related to neurotransmission (the GABA transporter GAT1 and the glutamate transporter GLT-1), these data allowed microsequencing or generation of specific antibodies that have been used to isolate cDNA clones (184, 424). Alternative strategies and serendipity allowed the identification of up to 22 c ...
... related to neurotransmission (the GABA transporter GAT1 and the glutamate transporter GLT-1), these data allowed microsequencing or generation of specific antibodies that have been used to isolate cDNA clones (184, 424). Alternative strategies and serendipity allowed the identification of up to 22 c ...
Engineering of polyketide biosynthetic pathways for bioactive
... shown a variety of biological activities. These molecules are synthesized by polyketide synthases (PKSs). PKSs are classified into three types based on their sequence, primary structure, and catalytic mechanism. Because of the bioactivities of polyketide natural products, this study is focused on th ...
... shown a variety of biological activities. These molecules are synthesized by polyketide synthases (PKSs). PKSs are classified into three types based on their sequence, primary structure, and catalytic mechanism. Because of the bioactivities of polyketide natural products, this study is focused on th ...
Flavour compounds in fungi
... Promoter analyses of the ARO genes in S. cerevisiae showed that the ScARO9 promoter region is directly regulated by the ScAro80 transcription factor. This interaction has been used to create a lacZ-reporter system to correlate the formation of two volatile compounds, 2phenylethanol and 2-phenylethyl ...
... Promoter analyses of the ARO genes in S. cerevisiae showed that the ScARO9 promoter region is directly regulated by the ScAro80 transcription factor. This interaction has been used to create a lacZ-reporter system to correlate the formation of two volatile compounds, 2phenylethanol and 2-phenylethyl ...
PDF - School of Chemical Sciences
... NAD(P)H is involved in approximately 80% of enzymatic reductions accounting for over 300 known reactions, many of which have potential in biocatalysis [13]. As a result, several regeneration systems for NAD(P)H have been described that allow cofactor addition in catalytic amounts [9,14–16]. Of these ...
... NAD(P)H is involved in approximately 80% of enzymatic reductions accounting for over 300 known reactions, many of which have potential in biocatalysis [13]. As a result, several regeneration systems for NAD(P)H have been described that allow cofactor addition in catalytic amounts [9,14–16]. Of these ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
... Scan-Prosite analysis predicted 3 potential N-glycosylation sites positioned at amino acids 250 to 253 (NISS), 306 to 309 (NLTH), and 399 to 402 (NSSS) of bSGLT2 (Figure 1). However, it is unclear whether these sites are actually glycosylated, because the in vitro translated product of bSGLT2 showed ...
... Scan-Prosite analysis predicted 3 potential N-glycosylation sites positioned at amino acids 250 to 253 (NISS), 306 to 309 (NLTH), and 399 to 402 (NSSS) of bSGLT2 (Figure 1). However, it is unclear whether these sites are actually glycosylated, because the in vitro translated product of bSGLT2 showed ...
幻灯片 1 - 湖北师范学院生命科学学院
... 2.5 Each amino acid is given a three-letter abbreviation and a oneletter symbol. They often the first three letter and the first letter. When there is confusion, an alternative is used. They must be remembered. (fig.) ...
... 2.5 Each amino acid is given a three-letter abbreviation and a oneletter symbol. They often the first three letter and the first letter. When there is confusion, an alternative is used. They must be remembered. (fig.) ...
Sequence variation characteristics of D
... can enter the intermembrane space, but most of them cannot pass the relatively impermeable inner membrane. Other proteins in this membrane include enzymes involved in mitochondrial lipid synthesis and enzymes that convert lipid substrates into forms that are subsequently metabolized in the matrix. T ...
... can enter the intermembrane space, but most of them cannot pass the relatively impermeable inner membrane. Other proteins in this membrane include enzymes involved in mitochondrial lipid synthesis and enzymes that convert lipid substrates into forms that are subsequently metabolized in the matrix. T ...
2.2. Garrido-Franco, M. Structure E. coli
... active site of the neighboring subunit. Arg20, which is part of the C-terminal loop of helix 1a, is even protruding into the adjacent active site, where it fulfills important mechanistic functions. Thus, the “shared” active site strongly suggests that the dimer is the active unit of PNP synthase. It ...
... active site of the neighboring subunit. Arg20, which is part of the C-terminal loop of helix 1a, is even protruding into the adjacent active site, where it fulfills important mechanistic functions. Thus, the “shared” active site strongly suggests that the dimer is the active unit of PNP synthase. It ...
Flux analysis in central carbon metabolism in plants
... composition and protein hydrolysis), Guy Sander and Chase Beisel (for taking up the baton in maintaining the genetically modified hairy root lines), Andrea Flack and Yingjun Wang (Martin Spalding group, for assistance with protein extraction). Also, thanks are due to my groupmatcs (past and present) ...
... composition and protein hydrolysis), Guy Sander and Chase Beisel (for taking up the baton in maintaining the genetically modified hairy root lines), Andrea Flack and Yingjun Wang (Martin Spalding group, for assistance with protein extraction). Also, thanks are due to my groupmatcs (past and present) ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.