Regulation of Starch Synthesis in Cassava Yona Baguma
... (Ball & Morell, 2003). It is a glucose polymer of α-glucans linked by α-1,4 bonds and branched at α-1,6 positions (Ball & Morell, 2003; Ball et al., 1996). It exists in the leaf chloroplasts as transitory starch (i.e. the primary product of photosynthesis) and in the amyloplasts as storage starch ( ...
... (Ball & Morell, 2003). It is a glucose polymer of α-glucans linked by α-1,4 bonds and branched at α-1,6 positions (Ball & Morell, 2003; Ball et al., 1996). It exists in the leaf chloroplasts as transitory starch (i.e. the primary product of photosynthesis) and in the amyloplasts as storage starch ( ...
Engineering the pentose phosphate pathway of
... The baker’s yeast Saccharomyces cerevisiae has a long tradition in alcohol production from D-glucose of e.g. starch. However, without genetic modifications it is unable to utilise the 5-carbon sugars D-xylose and L-arabinose present in plant biomass. In this study, one key metabolic step of the cata ...
... The baker’s yeast Saccharomyces cerevisiae has a long tradition in alcohol production from D-glucose of e.g. starch. However, without genetic modifications it is unable to utilise the 5-carbon sugars D-xylose and L-arabinose present in plant biomass. In this study, one key metabolic step of the cata ...
Raines, ChemRev 1998
... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
Spectroscopic Comparisons of the pH Dependencies of Fe
... residues are crucial to the nature of these residues’ interactions with each other and their influence on the activity of the metal ion. Despite their importance in determining the metal center activity, the effects of second sphere interactions are subtler than those of ligands, and not nearly as w ...
... residues are crucial to the nature of these residues’ interactions with each other and their influence on the activity of the metal ion. Despite their importance in determining the metal center activity, the effects of second sphere interactions are subtler than those of ligands, and not nearly as w ...
On the role and formation of covalently bound flavin cofactors Heuts
... been described for the NqrB and NqrC subunits of the Na+-translocating NADH-quinone reductase (Na+NQR) from Vibrio alginolyticus. In this case, FMN is covalently linked to a threonine residue via a phosphoester bond [22]. Consequently, it represents the only covalent flavin–protein bond that does not ...
... been described for the NqrB and NqrC subunits of the Na+-translocating NADH-quinone reductase (Na+NQR) from Vibrio alginolyticus. In this case, FMN is covalently linked to a threonine residue via a phosphoester bond [22]. Consequently, it represents the only covalent flavin–protein bond that does not ...
Functional characterisation of the TUP5 gene in - diss.fu
... threshold of callus induction in the wild type, while the tumor prone mutants produced calli (Riefler, 2001). Furthermore, tup5-1 also has a unique blue light-dependent short root phenotype: The root meristem is lost under blue light irradiation, resulting in root growth stop in the mutant (Frémont, ...
... threshold of callus induction in the wild type, while the tumor prone mutants produced calli (Riefler, 2001). Furthermore, tup5-1 also has a unique blue light-dependent short root phenotype: The root meristem is lost under blue light irradiation, resulting in root growth stop in the mutant (Frémont, ...
Localization and nucleotide specificity of Blastocystis succinyl‐CoA
... infecting up to 10% of the developed world (Stenzel and Boreham, 1996). However, the role Blastocystis has in causing actual disease, if any, is still a matter of dispute. It is one of the few known human parasites within the stramenopiles (Silberman et al., 1996), a group also containing, for examp ...
... infecting up to 10% of the developed world (Stenzel and Boreham, 1996). However, the role Blastocystis has in causing actual disease, if any, is still a matter of dispute. It is one of the few known human parasites within the stramenopiles (Silberman et al., 1996), a group also containing, for examp ...
Engineering of Aromatic Amino Acid Metabolism in
... sucrose or glucose. Batch or fed-batch cultivation is the preferable type of process for amino acid production. Lphenylalanine production by E. coli by fed-batch process which resulted in a titer of 34 g.l-1 can be given as an example (31). The strains used in amino acid fermentations are a very imp ...
... sucrose or glucose. Batch or fed-batch cultivation is the preferable type of process for amino acid production. Lphenylalanine production by E. coli by fed-batch process which resulted in a titer of 34 g.l-1 can be given as an example (31). The strains used in amino acid fermentations are a very imp ...
Viva Voce : Orals in Biochemistry
... 10. Chemistry and metabolism of nucleic acids. ............................................................ 83 11. (i) Molecular biology – I – A Replication ............................................................ 93 (ii) Molecular biology I – B Transcription .................................... ...
... 10. Chemistry and metabolism of nucleic acids. ............................................................ 83 11. (i) Molecular biology – I – A Replication ............................................................ 93 (ii) Molecular biology I – B Transcription .................................... ...
PKU - Nutricia Learning Center
... infant with classic PKU, poorly controlled PHE levels will result in irreversible brain damage. Therefore, it is essential that each infant who is diagnosed with PKU be started on a low-PHE diet to lower and maintain PHE levels within an appropriate range as soon after birth as possible. If a child ...
... infant with classic PKU, poorly controlled PHE levels will result in irreversible brain damage. Therefore, it is essential that each infant who is diagnosed with PKU be started on a low-PHE diet to lower and maintain PHE levels within an appropriate range as soon after birth as possible. If a child ...
papain, a plant enzyme of biological importance
... sulfate, SDS at different concentrations has exhibited that hydrophobic interactions play an important role in inducing the two different intermediates along the two various thermodynamic pathways (Chamani et al., 2009). Catalytic activity of papain involves hydrolysis of proteins with broad specifi ...
... sulfate, SDS at different concentrations has exhibited that hydrophobic interactions play an important role in inducing the two different intermediates along the two various thermodynamic pathways (Chamani et al., 2009). Catalytic activity of papain involves hydrolysis of proteins with broad specifi ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... gastrointestinal pathogens (Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Enterobacter aerogenes and Bacillus cereus) using the agar well diffusion assay. The qualitative screening of phytochemicals in both the aqueous and ethanol leaf extracts of Moringa oleifera showed the presence o ...
... gastrointestinal pathogens (Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Enterobacter aerogenes and Bacillus cereus) using the agar well diffusion assay. The qualitative screening of phytochemicals in both the aqueous and ethanol leaf extracts of Moringa oleifera showed the presence o ...
Membrane transporters in a human genome-scale
... et al., 2013), is a substantial expansion over Recon 1 and includes more than 370 additional transport and exchange reactions/systems. These global reconstructions do not represent the metabolic capability of a single cell or tissue but rather are blueprints for all human cells. This reconstruction ...
... et al., 2013), is a substantial expansion over Recon 1 and includes more than 370 additional transport and exchange reactions/systems. These global reconstructions do not represent the metabolic capability of a single cell or tissue but rather are blueprints for all human cells. This reconstruction ...
Organization and Integration of Large-scale Datasets for
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
Wax ester fermentation and fatty acid biosynthesis in the facultatively
... level was very high. The advent of oxygen took place during the time from 2.5 to ca 0.6 Ga (Canfield 1998; Anbar and Knoll 2001; Canfield et al. 2007) and the eukaryotes must have arisen and diversified by that time at very low oxygen tension and high sulfide concentration as anaerobic organisms (Ma ...
... level was very high. The advent of oxygen took place during the time from 2.5 to ca 0.6 Ga (Canfield 1998; Anbar and Knoll 2001; Canfield et al. 2007) and the eukaryotes must have arisen and diversified by that time at very low oxygen tension and high sulfide concentration as anaerobic organisms (Ma ...
Organization and Integration of Large
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
Unusual ADP-forming acetyl-coenzyme A synthetases from the
... So far, ACDs in archaea have been characterized only from the anaerobic, hyperthermophilic euryarchaeota P. furiosus, Archaeoglobus fulgidus and Methanococcus jannaschii (Glasemacher et al. 1997; Mai and Adams 1996; Musfeldt et al. 1999; Musfeldt and Schönheit 2002). In P. furiosus, two isoenzymes, ...
... So far, ACDs in archaea have been characterized only from the anaerobic, hyperthermophilic euryarchaeota P. furiosus, Archaeoglobus fulgidus and Methanococcus jannaschii (Glasemacher et al. 1997; Mai and Adams 1996; Musfeldt et al. 1999; Musfeldt and Schönheit 2002). In P. furiosus, two isoenzymes, ...
ALIMET® and DL-methionine solubility in extruded aquatic feeds
... years, nutritionists have started to incorporate vegetable-based protein sources like soybean meal and corn gluten meal in the diets of aquatic species. However, the amino acid profile of the vegetable protein sources is not adequate for the high demands of these commercially grown aquatic species a ...
... years, nutritionists have started to incorporate vegetable-based protein sources like soybean meal and corn gluten meal in the diets of aquatic species. However, the amino acid profile of the vegetable protein sources is not adequate for the high demands of these commercially grown aquatic species a ...
PDF hosted at the Radboud Repository of the Radboud University
... to its total magnitude (146, 191, 243). This means that Dc can be considered a key indicator of mitochondrial function, health, and metabolic activity (68, 254). At complex V (F0=F1ATP-synthase or CV; EC 3.6.1.34), protons are allowed to flow back into the mitochondrial matrix, thereby driving the s ...
... to its total magnitude (146, 191, 243). This means that Dc can be considered a key indicator of mitochondrial function, health, and metabolic activity (68, 254). At complex V (F0=F1ATP-synthase or CV; EC 3.6.1.34), protons are allowed to flow back into the mitochondrial matrix, thereby driving the s ...
Influence of oscillating dietary crude protein concentration on
... ity. Feeding cattle in confinement can lead to the concentration of nutrients, such as N and P. Ideally, most of these nutrients will be captured in manure that is subsequently used as a fertilizer for crops. However, significant quantities of N can be lost to the atmosphere (Bierman et al., 1999) a ...
... ity. Feeding cattle in confinement can lead to the concentration of nutrients, such as N and P. Ideally, most of these nutrients will be captured in manure that is subsequently used as a fertilizer for crops. However, significant quantities of N can be lost to the atmosphere (Bierman et al., 1999) a ...
Mechanistic Role of an NS4A Peptide Cofactor with the Truncated
... the intermolecular consensus sequence site2 D/E-X-X-X-XC*-A/S differs from the intramolecular cleavage site by substitution of Thr for Cys at the P1 position (Grakoui et al., 1993a; Pizzi et al., 1994). A body of experimental evidence demonstrated that the 70 kDa NS3 protein is composed of two domai ...
... the intermolecular consensus sequence site2 D/E-X-X-X-XC*-A/S differs from the intramolecular cleavage site by substitution of Thr for Cys at the P1 position (Grakoui et al., 1993a; Pizzi et al., 1994). A body of experimental evidence demonstrated that the 70 kDa NS3 protein is composed of two domai ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.