Regulation of mitochondrial calcium in plants versus animals
... intermolecular disulphide bond and closes the channel at low extramitochondrial Ca2+ concentrations (Patron et al., 2014; Petrungaro et al., 2015). The stability of MICU2 depends on MICU1 (Plovanich et al., 2013; Patron et al., 2014), and loss of MICU2 in MICU1-silenced cells complicates assignment ...
... intermolecular disulphide bond and closes the channel at low extramitochondrial Ca2+ concentrations (Patron et al., 2014; Petrungaro et al., 2015). The stability of MICU2 depends on MICU1 (Plovanich et al., 2013; Patron et al., 2014), and loss of MICU2 in MICU1-silenced cells complicates assignment ...
Ca2+-Dependent Regulations and Signaling in Skeletal Muscle
... describes the interplay between an action potential which is induced by the nervous system and the resulting molecular interaction of myofilaments leading finally to mechanical force development and shortening of sarcomeres. The multiple roles of Ca2+ ions in the regulation of acute contraction of s ...
... describes the interplay between an action potential which is induced by the nervous system and the resulting molecular interaction of myofilaments leading finally to mechanical force development and shortening of sarcomeres. The multiple roles of Ca2+ ions in the regulation of acute contraction of s ...
Characterization of Agouti-Related Protein
... and efficacy as baculovirus-produced human AGRP Form A 1 B. In cAMP assays [Leu127Pro] AGRP, AGRP(87–132), and recombinant human AGRP Form A 1 B are essentially equipotent at inhibiting the hMC4R. Both chemically synthesized variant AGRP molecules were also found to display a similar nanomolar range ...
... and efficacy as baculovirus-produced human AGRP Form A 1 B. In cAMP assays [Leu127Pro] AGRP, AGRP(87–132), and recombinant human AGRP Form A 1 B are essentially equipotent at inhibiting the hMC4R. Both chemically synthesized variant AGRP molecules were also found to display a similar nanomolar range ...
Physiological Strategies During Animal Diapause: Lessons from
... embryos are introduced into the brood pouch and then released into the lake. These embryos enter diapause at the gastrula stage (Clegg and Conte, 1980). Although the developmental cessation is complete by the time embryos are released by the female into the water column, the metabolic depression req ...
... embryos are introduced into the brood pouch and then released into the lake. These embryos enter diapause at the gastrula stage (Clegg and Conte, 1980). Although the developmental cessation is complete by the time embryos are released by the female into the water column, the metabolic depression req ...
Glycerol transport and phosphoenolpyruvate
... serine were able to grow on glycerol as the sole carbon source, even when the general PTS proteins enzyme I and HPr were not functional (Wehtje et al., 1995). Glycerol kinase in these mutants was therefore assumed to possess elevated activity, rendering glycerol uptake and metabolism independent of ...
... serine were able to grow on glycerol as the sole carbon source, even when the general PTS proteins enzyme I and HPr were not functional (Wehtje et al., 1995). Glycerol kinase in these mutants was therefore assumed to possess elevated activity, rendering glycerol uptake and metabolism independent of ...
Mutational and spectroscopic studies of the significance of the active
... MnSOD (sodA) was cloned into the overexpression vector pET24d (Novagen) using standard techniques and mutagenized by PCR using the megaprimer method [21]. Both strands of the mutant gene were completely sequenced to confirm the presence of the desired Gly77 to Gln and Gln146 to Ala mutations, and th ...
... MnSOD (sodA) was cloned into the overexpression vector pET24d (Novagen) using standard techniques and mutagenized by PCR using the megaprimer method [21]. Both strands of the mutant gene were completely sequenced to confirm the presence of the desired Gly77 to Gln and Gln146 to Ala mutations, and th ...
Pyruvate dehydrogenase - Wageningen UR E
... complexes. For a better understanding however, the most important features of the other components of the complex will be discussed in this section [for reviews see Perham, 1991; Mattevi etal., 1992a;Berg&deKok, 1997;deKok etal., 1998;Perham,2001]. Limited proteolysis studies revealed that E2 has a ...
... complexes. For a better understanding however, the most important features of the other components of the complex will be discussed in this section [for reviews see Perham, 1991; Mattevi etal., 1992a;Berg&deKok, 1997;deKok etal., 1998;Perham,2001]. Limited proteolysis studies revealed that E2 has a ...
View/Open
... represent the fastest growing segment of the fuel ethanol industry in the U.S., and produce the majority (60%) of fuel ethanol. By-products from dry-grind ethanol plants include wet and dry distiller’s grains, wet and dried distiller’s grains with solubles, modified “wet cake” (blend of condensed di ...
... represent the fastest growing segment of the fuel ethanol industry in the U.S., and produce the majority (60%) of fuel ethanol. By-products from dry-grind ethanol plants include wet and dry distiller’s grains, wet and dried distiller’s grains with solubles, modified “wet cake” (blend of condensed di ...
Molecular and biochemical characterization of cytosolic
... using a random primer (Stratagene). Filters were washed under low stringency conditions for three 30 min washes using 23 SSC and 0.1% SDS at 50 °C. RNA isolation and hybridization Total RNA was isolated from different developmental stages (5±30 dpa) of wheat endosperm according to the method describ ...
... using a random primer (Stratagene). Filters were washed under low stringency conditions for three 30 min washes using 23 SSC and 0.1% SDS at 50 °C. RNA isolation and hybridization Total RNA was isolated from different developmental stages (5±30 dpa) of wheat endosperm according to the method describ ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
... Hyperthermophilic organisms have optimum growth temperatures above 80°C and belong to genera that are placed near the root of the Tree of Life, in short phylogenetic branches within the domains Bacteria or Archaea. Although hyperthermophiles have been isolated from a variety of hot environments, mos ...
... Hyperthermophilic organisms have optimum growth temperatures above 80°C and belong to genera that are placed near the root of the Tree of Life, in short phylogenetic branches within the domains Bacteria or Archaea. Although hyperthermophiles have been isolated from a variety of hot environments, mos ...
Investigating genotype-phenotype relationships in
... For accomplishing the multifaceted venture that was my Ph.D., I was very fortunate to have Prof. Uffe H. Mortensen and Kiran R. Patil as my supervisors, to whom I gladly thank for leading me through this experience. Without Uffe’s support, who introduced me to molecular biology and yeast genetics, i ...
... For accomplishing the multifaceted venture that was my Ph.D., I was very fortunate to have Prof. Uffe H. Mortensen and Kiran R. Patil as my supervisors, to whom I gladly thank for leading me through this experience. Without Uffe’s support, who introduced me to molecular biology and yeast genetics, i ...
Structure and Function of Thymosin β4
... containing fluorinated alcohols6 such as TFE. Therefore the helices in thymosin β4 of residues 4 – 16 and 30 – 40 were more pronounced in 60% TFE solution. The CD measurements of wild type thymosin β4 showed a maximum at a wavelength of 190 nm and double minima at wavelengths 207 nm and 222 nm, whic ...
... containing fluorinated alcohols6 such as TFE. Therefore the helices in thymosin β4 of residues 4 – 16 and 30 – 40 were more pronounced in 60% TFE solution. The CD measurements of wild type thymosin β4 showed a maximum at a wavelength of 190 nm and double minima at wavelengths 207 nm and 222 nm, whic ...
Identification and first characterization of pairing
... http://www.who.int/mediacentre/factsheets/fs115/en), while approximately 800 million are at risk (Ross et al., 2012). Besides the resulting and underestimated socio economic importance (King, 2010), schistosomes are also of basic economic relevance as they can infect animals as well (Quack et al., 2 ...
... http://www.who.int/mediacentre/factsheets/fs115/en), while approximately 800 million are at risk (Ross et al., 2012). Besides the resulting and underestimated socio economic importance (King, 2010), schistosomes are also of basic economic relevance as they can infect animals as well (Quack et al., 2 ...
Functional Interactions Between the Subunits of the Lactose
... A covalent dimer to study subunit interactions Functional interactions between subunits in dimeric proteins have been studied by mixing active and inactive species in different ratios and characterizing the resulting heterodimers (7,103,119,124), which constitute at most 50% of all dimers, that is, ...
... A covalent dimer to study subunit interactions Functional interactions between subunits in dimeric proteins have been studied by mixing active and inactive species in different ratios and characterizing the resulting heterodimers (7,103,119,124), which constitute at most 50% of all dimers, that is, ...
ARTICLES
... canonical Watson-Click base pair, however, this decoding does not generally alter the identity of the amino acid in the peptide, and thus its fidelity is maintained1. On the other hand, if a U G mispair at the first or second base pair occurs, such a codon-anticodon interaction does accompany an ami ...
... canonical Watson-Click base pair, however, this decoding does not generally alter the identity of the amino acid in the peptide, and thus its fidelity is maintained1. On the other hand, if a U G mispair at the first or second base pair occurs, such a codon-anticodon interaction does accompany an ami ...
Effects of low crude-protein diets fortified with crystalline amino acids
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from ...
... Corn and soybean meal (SBM) are included in most pig and broiler diets as sources of protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from ...
Identification and Cloning of a Cryptococcal Deacetylase That
... sequence, a DNA probe was used to screen a X cDNA library prepared from strain 63501. Clones were isolated containing thè full-length gene (d25), which showed homology with a number of polysaccharìde deacetylases from fungi and bacteria. The recombinant d25 protein expressed in Escherìchia coli was ...
... sequence, a DNA probe was used to screen a X cDNA library prepared from strain 63501. Clones were isolated containing thè full-length gene (d25), which showed homology with a number of polysaccharìde deacetylases from fungi and bacteria. The recombinant d25 protein expressed in Escherìchia coli was ...
Eram_SeyedMohammad - UWSpace
... neapolitana, and P. furiosus) had the highest AHAS activity at nearly neutral pH, but the ones without ilv operon still had some residual AHAS activity mostly at a higher pH value (pH 10.5). It was shown that the cell-free extracts of T. maritima can catalyze the non-oxidative decarboxylation of the ...
... neapolitana, and P. furiosus) had the highest AHAS activity at nearly neutral pH, but the ones without ilv operon still had some residual AHAS activity mostly at a higher pH value (pH 10.5). It was shown that the cell-free extracts of T. maritima can catalyze the non-oxidative decarboxylation of the ...
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed
... 4-hydroxylase, C4H21, p-coumaric acid 3-hydroxylase, C3H22, but is not clear whether CoA-derivatives are involved or whether the C3-hydroxylation step proceeds, for example, through quinate- and shikimate esters22. Caffeic acid could in principle be O-methylated23 to form ferulic acid, the substrate ...
... 4-hydroxylase, C4H21, p-coumaric acid 3-hydroxylase, C3H22, but is not clear whether CoA-derivatives are involved or whether the C3-hydroxylation step proceeds, for example, through quinate- and shikimate esters22. Caffeic acid could in principle be O-methylated23 to form ferulic acid, the substrate ...
Structural elements defining elongation factor Tu mediated
... [3H]-labeled aa-tRNA and EF-Tu GTP in concentrations ranging from 0.02 to 2 mM in 50 mM Na-Hepes pH 7.2, 5 mM NH4Cl, 50 mM KCl, 10 mM MgCl2 and 1 mM GTP. After 5 min incubation at 48C, the mixture was filtered on a nitrocellulose disc presoaked in washing buffer. Filters were washed with 400 ml of r ...
... [3H]-labeled aa-tRNA and EF-Tu GTP in concentrations ranging from 0.02 to 2 mM in 50 mM Na-Hepes pH 7.2, 5 mM NH4Cl, 50 mM KCl, 10 mM MgCl2 and 1 mM GTP. After 5 min incubation at 48C, the mixture was filtered on a nitrocellulose disc presoaked in washing buffer. Filters were washed with 400 ml of r ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.