Functional and Biochemical Analysis of Glucose-6
... (GST)/G6PD fusion protein in Escherichia coli; and observed that the values of kinetic parameters from the biochemical characterization of the resulting proteins were the same as those reported using the initial vectors. These results revealed that this new system was very useful for the expression ...
... (GST)/G6PD fusion protein in Escherichia coli; and observed that the values of kinetic parameters from the biochemical characterization of the resulting proteins were the same as those reported using the initial vectors. These results revealed that this new system was very useful for the expression ...
Purification and Characterization of Chorismate
... monitoring the anthranilate production. Throughout the enzyme purification, the discontinuous coupled assay was used, according to ref. 32 for E. gracilis and N. crassa, and according to ref. 28 for C. sempervirens and E. coli. The coupled assay was not used for the characterization of chorismate sy ...
... monitoring the anthranilate production. Throughout the enzyme purification, the discontinuous coupled assay was used, according to ref. 32 for E. gracilis and N. crassa, and according to ref. 28 for C. sempervirens and E. coli. The coupled assay was not used for the characterization of chorismate sy ...
The metabolic basis of Candida albicans
... pathway and cAMP-PKA pathway. These lead to the activation of transcriptional regulators Cph1p and Efg1p, respectively, which promote filamentous growth. Nitrogen starvation activates both the MAP Kinase and cAMP-PKA pathways via ammonium permease (Mep2p). Adenylyl cyclase (Cdc35p) not only responds ...
... pathway and cAMP-PKA pathway. These lead to the activation of transcriptional regulators Cph1p and Efg1p, respectively, which promote filamentous growth. Nitrogen starvation activates both the MAP Kinase and cAMP-PKA pathways via ammonium permease (Mep2p). Adenylyl cyclase (Cdc35p) not only responds ...
Biochemical studies of enzymes in insect cuticle hardening
... and the pathway of insect taurine biosynthesis. Cysteine was found to be an inactivator of several PLP-dependent decarboxylases, such as ADC, glutamate decarboxylase (GAD) and CSADC. This study helps to understand symptoms associated with the abnormal cysteine concentrations in several neurodegener ...
... and the pathway of insect taurine biosynthesis. Cysteine was found to be an inactivator of several PLP-dependent decarboxylases, such as ADC, glutamate decarboxylase (GAD) and CSADC. This study helps to understand symptoms associated with the abnormal cysteine concentrations in several neurodegener ...
The Unusual Binding Properties of the Third Distinct Teleost
... an ER␥ (18), we designated this new subtype acER␥ (17). The ER␥ subtype is present in other teleosts and arose from a gene duplication of ER early in the teleost lineage after the divergence of ray- and lobe-finned fishes. These three subtypes were subsequently identified in zebrafish (19). The acE ...
... an ER␥ (18), we designated this new subtype acER␥ (17). The ER␥ subtype is present in other teleosts and arose from a gene duplication of ER early in the teleost lineage after the divergence of ray- and lobe-finned fishes. These three subtypes were subsequently identified in zebrafish (19). The acE ...
Analysis of the Arabidopsis rsr4-1/pdx1
... that constituents of the TCA cycle, such as malate, citrate, and fumarate, were highly upregulated (Figure 4B) and that shikimate, a key compound in indole-3-acetic acid (IAA) biosynthesis, was significantly reduced. Finally, relatively few changes in carbohydrate content were observed in the mutant ...
... that constituents of the TCA cycle, such as malate, citrate, and fumarate, were highly upregulated (Figure 4B) and that shikimate, a key compound in indole-3-acetic acid (IAA) biosynthesis, was significantly reduced. Finally, relatively few changes in carbohydrate content were observed in the mutant ...
Multilevel functional and structural defects induced by two
... The two isoacceptors hmtRNALeu (UUR) and hmtRNALeu (CUN) have a classical cloverleaf structure and a short variable arm in contrast to bacterial tRNALeu , which displays the long variable arm characteristic of class II tRNAs (Figures 1A and 1B). In a preliminary study, we explored the aminoacylation ...
... The two isoacceptors hmtRNALeu (UUR) and hmtRNALeu (CUN) have a classical cloverleaf structure and a short variable arm in contrast to bacterial tRNALeu , which displays the long variable arm characteristic of class II tRNAs (Figures 1A and 1B). In a preliminary study, we explored the aminoacylation ...
Document
... denaturation in 8 M urea at a slightly than the insulin portion. Thus, while acalkaline pH, the single peptide chain of cepted point mutations occur at a rate of proinsulin rapidly reoxidizes to its original approximately four per hundred residues disulfide bond structure in high yield when per mill ...
... denaturation in 8 M urea at a slightly than the insulin portion. Thus, while acalkaline pH, the single peptide chain of cepted point mutations occur at a rate of proinsulin rapidly reoxidizes to its original approximately four per hundred residues disulfide bond structure in high yield when per mill ...
Emerging role of Nrf2 - Discovery
... in Nrf2-KO cells and tissues compared to their WT counterparts. However, we made the unexpected observation that, compared to WT, Keap1-KD cells also have higher rates of ROS production, although the magnitude of the difference between the WT and the Keap1-KD genotypes was smaller than that between ...
... in Nrf2-KO cells and tissues compared to their WT counterparts. However, we made the unexpected observation that, compared to WT, Keap1-KD cells also have higher rates of ROS production, although the magnitude of the difference between the WT and the Keap1-KD genotypes was smaller than that between ...
Complete genome sequence of the prototype lactic acid bacterium
... by employing UV treatment and protoplast-curing strategies in the early 1980s (41). The resultant plasmid-free strain, L. lactis MG1363, is robust and genetically amenable, which has facilitated the analysis of introduced lactococcal and heterologous DNA. Sophisticated systems have been developed fo ...
... by employing UV treatment and protoplast-curing strategies in the early 1980s (41). The resultant plasmid-free strain, L. lactis MG1363, is robust and genetically amenable, which has facilitated the analysis of introduced lactococcal and heterologous DNA. Sophisticated systems have been developed fo ...
Sequence Similarity Searching: Understanding and Using Web
... • Similarity can be indicative of homology • Generally, if two sequences are significantly similar over entire length they are likely homologous • Low complexity regions can be highly similar without being homologous • Homologous sequences not always highly similar • Suggested BLAST Cutoffs ...
... • Similarity can be indicative of homology • Generally, if two sequences are significantly similar over entire length they are likely homologous • Low complexity regions can be highly similar without being homologous • Homologous sequences not always highly similar • Suggested BLAST Cutoffs ...
The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros
... be recovered from poliovirus RNA chains varying in length from 7,50C nucleotides (full-sized RNA) to about 500 nucleotides. ...
... be recovered from poliovirus RNA chains varying in length from 7,50C nucleotides (full-sized RNA) to about 500 nucleotides. ...
NON-RADIOACTIVE ASSAY FOR ACETYL-CoA
... But whereas the citrate synthase reaction in the pyruvate carboxylase assay contains excess acetylCoA and measures how much oxaloacetate was produced in the first reaction, the citrate synthase reaction for this assay contains excess oxaloacetate and measures how much acetyl-CoA was consumed in the ...
... But whereas the citrate synthase reaction in the pyruvate carboxylase assay contains excess acetylCoA and measures how much oxaloacetate was produced in the first reaction, the citrate synthase reaction for this assay contains excess oxaloacetate and measures how much acetyl-CoA was consumed in the ...
Lectins, as non-self-recognition factors, in crustaceans
... self-glycoproteins ŽThiel and Reid, 1989.. This observation is related to the equatorial orientation of the C3–OH and C4–OH groups of the sugar moiety ŽWeis and Drickamer, 1996., a configuration represented in the hexoses N-acetylglucosamine ŽGlcNAc., glucose and fucose as well as mannose. Combinati ...
... self-glycoproteins ŽThiel and Reid, 1989.. This observation is related to the equatorial orientation of the C3–OH and C4–OH groups of the sugar moiety ŽWeis and Drickamer, 1996., a configuration represented in the hexoses N-acetylglucosamine ŽGlcNAc., glucose and fucose as well as mannose. Combinati ...
Fast evolutionary rates associated with functional loss in class I
... identified. The sgtp2 open reading frame was interrupted by a missing base ~260 bp downstream the first initiator ATG codon. The hypothetical addition of a base at this downstream position resulted in a translation product homologous to glucose transporters [4]. Functional assays using Xenopus oocyt ...
... identified. The sgtp2 open reading frame was interrupted by a missing base ~260 bp downstream the first initiator ATG codon. The hypothetical addition of a base at this downstream position resulted in a translation product homologous to glucose transporters [4]. Functional assays using Xenopus oocyt ...
43803_C000 1..20
... GH, IGF-1, Insulin, and Amino Acids Synergism ..................................................................31 Nutrient and Hormone Delivery to Muscle .............................................................................32 Growth Hormone and Exercise ..................................... ...
... GH, IGF-1, Insulin, and Amino Acids Synergism ..................................................................31 Nutrient and Hormone Delivery to Muscle .............................................................................32 Growth Hormone and Exercise ..................................... ...
ESTUDIO DE LOS MECANISMOS DE INHIBICIÓN DE LA ACTIVIDAD CARNITINA PALMITOILTRANSFERASA 1
... (SequenceSpace) and multivariate statistics, principal component analysis, to allow reduction of the number of dimensions. This representation allows us not only to define clusters of proteins according to specific properties by choosing the appropriate axes defined by the highest corresponding eige ...
... (SequenceSpace) and multivariate statistics, principal component analysis, to allow reduction of the number of dimensions. This representation allows us not only to define clusters of proteins according to specific properties by choosing the appropriate axes defined by the highest corresponding eige ...
Full-Text PDF
... and acatalasemia) [8] to more common age-related disorders such as diabetes, neurodegenerative disease, and cancer [10,11]. Like peroxisomes, mitochondria are also dynamic organelles that continuously adapt their number, morphology, and function to prevailing environmental conditions [12]. In mammal ...
... and acatalasemia) [8] to more common age-related disorders such as diabetes, neurodegenerative disease, and cancer [10,11]. Like peroxisomes, mitochondria are also dynamic organelles that continuously adapt their number, morphology, and function to prevailing environmental conditions [12]. In mammal ...
A Comparison of Murine and Human Factor XI
... that activates factor IX by limited proteolysis.1 The importance of this protein in normal hemostasis is demonstrated by the hemorrhagic diathesis associated with congenital factor XI deficiency in humans, dogs, and cattle.2-4 The physiologic mechanisms by which factor XI is activated and the contri ...
... that activates factor IX by limited proteolysis.1 The importance of this protein in normal hemostasis is demonstrated by the hemorrhagic diathesis associated with congenital factor XI deficiency in humans, dogs, and cattle.2-4 The physiologic mechanisms by which factor XI is activated and the contri ...
Late events of translation initiation in bacteria: a kinetic analysis
... changes of pro¯avin in position 16/17 of the tRNA were used to monitor the conformational changes during initial binding of the EF-Tu ternary complex, codon recognition and release of aa-tRNA from EF-Tu followed by accommodation in the A-site (Figure 3B). Fluorescence changes of the GTP derivative, ...
... changes of pro¯avin in position 16/17 of the tRNA were used to monitor the conformational changes during initial binding of the EF-Tu ternary complex, codon recognition and release of aa-tRNA from EF-Tu followed by accommodation in the A-site (Figure 3B). Fluorescence changes of the GTP derivative, ...
Nicotinamide adenine dinucleotide, a metabolic regulator of
... components of the de novo pathway reside evenly throughout the cell and have no effect on silencing [14,15]. NAD also regulates gene expression at other genomic loci. Clock/BMAL1 and NPAS2/BMAL1 are heterodimeric transcription factors that regulate the expression of genes controlling the circadian ...
... components of the de novo pathway reside evenly throughout the cell and have no effect on silencing [14,15]. NAD also regulates gene expression at other genomic loci. Clock/BMAL1 and NPAS2/BMAL1 are heterodimeric transcription factors that regulate the expression of genes controlling the circadian ...
Intracellular Localization of Interleukin-6 in
... for EPO, ECP (Fig 3), and MBP, and was usually confined to a single peak. We have previously shown that the pellet produced from pooled fractions corresponding to peak granule protein activity contains enriched secretory granules.5 The plasma membrane and cytosol detected by peak antiCD9 binding and ...
... for EPO, ECP (Fig 3), and MBP, and was usually confined to a single peak. We have previously shown that the pellet produced from pooled fractions corresponding to peak granule protein activity contains enriched secretory granules.5 The plasma membrane and cytosol detected by peak antiCD9 binding and ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.