Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
... growing steadily. The most remarkable use of L-threonine is as a feed additive, as recent studies have acknowledged L-threonine as the second limiting amino acid in swine feeds after L-lysine and the third limiting amino acid in poultry feeds after L-lysine and L-methionine (Ajinomoto, 2009). Applic ...
... growing steadily. The most remarkable use of L-threonine is as a feed additive, as recent studies have acknowledged L-threonine as the second limiting amino acid in swine feeds after L-lysine and the third limiting amino acid in poultry feeds after L-lysine and L-methionine (Ajinomoto, 2009). Applic ...
aminoacids
... Undergo digestion to split into aminoacids The least preferred role in energy generation Perform a large number of roles, however, major role in structure and catalysis ...
... Undergo digestion to split into aminoacids The least preferred role in energy generation Perform a large number of roles, however, major role in structure and catalysis ...
The uterine tubal fluid: secretion, composition and biological effects
... The uterine tube provides the appropriate environment for oocytes, spermatozoa transport, fertilization, and early embryo development. When attempts to reproduce any of these events outside the tubal lumen are made, dramatic drops in efficiency are consistently seen. This limitation is particularly ...
... The uterine tube provides the appropriate environment for oocytes, spermatozoa transport, fertilization, and early embryo development. When attempts to reproduce any of these events outside the tubal lumen are made, dramatic drops in efficiency are consistently seen. This limitation is particularly ...
Systems Biotechnology of Pseudomonas putida for the enhanced
... along with a systems biotechnology approach with the aim of enhancing the production of PHAs. First, it was shown that P. putida and related species are suitable as whole-cell biocatalyzers for the production of several value-added industrial compounds. To highlight differences among various nutrien ...
... along with a systems biotechnology approach with the aim of enhancing the production of PHAs. First, it was shown that P. putida and related species are suitable as whole-cell biocatalyzers for the production of several value-added industrial compounds. To highlight differences among various nutrien ...
New insight into the photoheterotrophic growth of the
... intermediate of the branched-chain amino acid biosynthesis pathway, which is activated during acetate assimilation, rather than a metabolite of the so-called citramalate cycle. ...
... intermediate of the branched-chain amino acid biosynthesis pathway, which is activated during acetate assimilation, rather than a metabolite of the so-called citramalate cycle. ...
Mechanisms of animal diapause: recent
... this remains to be demonstrated. The mechanisms associated with this effect have not been fully characterized, but they must act downstream of all three genetic pathways that control dauer entry, as the suppression has not been shown to be pathwayspecific. AMPK as a downstream effector. Just as DAF- ...
... this remains to be demonstrated. The mechanisms associated with this effect have not been fully characterized, but they must act downstream of all three genetic pathways that control dauer entry, as the suppression has not been shown to be pathwayspecific. AMPK as a downstream effector. Just as DAF- ...
Document
... isomers of spin labeled stearic acid, doxyl stearates, was applied to study the caffeine-HSA interaction in further detail. It was found that upon caffeine binding, the hyperfine splitting decreases for HSA labeled with 5-doxylstearate. This phenomenon may indicate either an increase in mobility or ...
... isomers of spin labeled stearic acid, doxyl stearates, was applied to study the caffeine-HSA interaction in further detail. It was found that upon caffeine binding, the hyperfine splitting decreases for HSA labeled with 5-doxylstearate. This phenomenon may indicate either an increase in mobility or ...
Structure-based design of inhibitors of NS3 serine protease
... The complex NS3/4A has been identified as a promising target for antiviral drugs effective against the HCV [15,19,20]. Recently, it has been reported that N-terminal cleavage products of the substrate form competitive inhibitors of the NS3 protease activity. These native inhibitors (typically hexape ...
... The complex NS3/4A has been identified as a promising target for antiviral drugs effective against the HCV [15,19,20]. Recently, it has been reported that N-terminal cleavage products of the substrate form competitive inhibitors of the NS3 protease activity. These native inhibitors (typically hexape ...
the thyroid and thyroid hormones
... The thyroid hormones T3 and T4 are formed in a large prohormone molecule, thyroglobulin, the major component of the thyroid and more precisely of the colloid. Thyroglobulin is synthesized in the thyroid follicular cells and secreted into the lumen of the follicles. It is an iodinated glycoprotein (6 ...
... The thyroid hormones T3 and T4 are formed in a large prohormone molecule, thyroglobulin, the major component of the thyroid and more precisely of the colloid. Thyroglobulin is synthesized in the thyroid follicular cells and secreted into the lumen of the follicles. It is an iodinated glycoprotein (6 ...
Catalytic Mechanism and Regulation of Mammalian Adenylyl Cyclases
... cAMP is a key player in the intracellular signaling pathways of hormones, neurotransmitters, odorants, and chemokines. By activating PKA and cyclic nucleotide-gated ion channels, this protean second messenger can change cellular attributes as diverse as the membrane potential and the rate of cell di ...
... cAMP is a key player in the intracellular signaling pathways of hormones, neurotransmitters, odorants, and chemokines. By activating PKA and cyclic nucleotide-gated ion channels, this protean second messenger can change cellular attributes as diverse as the membrane potential and the rate of cell di ...
Systematic metabolic analysis of recombinant Pichia pastoris UNIVERSITAT AUTÒNOMA DE BARCELONA
... Chapter 5, the redox and energy stoichiometry of P. pastoris was validated showing increasing energy requirements as the oxygen availability became shortage. In addition, a network-embedded thermodynamic analysis of the quantitative intracellular metabolites pools was performed. From the analysis, ...
... Chapter 5, the redox and energy stoichiometry of P. pastoris was validated showing increasing energy requirements as the oxygen availability became shortage. In addition, a network-embedded thermodynamic analysis of the quantitative intracellular metabolites pools was performed. From the analysis, ...
animal nutrition - e
... Cole D J A 1994); Figure 16.4 after The Energy Metabolism of Ruminants, Hutchinson (Blaxter K L 1967) p. 259 reproduced by permission of the publisher and the author; Figure 16.7 adapted from A review of the potential of nutrition to modify milk fat and protein, Livestock Production Science 23 (3–4) ...
... Cole D J A 1994); Figure 16.4 after The Energy Metabolism of Ruminants, Hutchinson (Blaxter K L 1967) p. 259 reproduced by permission of the publisher and the author; Figure 16.7 adapted from A review of the potential of nutrition to modify milk fat and protein, Livestock Production Science 23 (3–4) ...
Amino acid homeostasis and signalling in mammalian cells and
... an active mTORC1 complex will promote protein biosynthesis and subdue autophagy, while an inactive mTORC1 complex does the opposite. It is noteworthy that mTORC1 integrates many signals through it being regulated by the Tuberous sclerosis complex 2 (TSC2) [9]. For instance, mTORC1 requires indirect ...
... an active mTORC1 complex will promote protein biosynthesis and subdue autophagy, while an inactive mTORC1 complex does the opposite. It is noteworthy that mTORC1 integrates many signals through it being regulated by the Tuberous sclerosis complex 2 (TSC2) [9]. For instance, mTORC1 requires indirect ...
Mammalian CSAD and GADL1 have distinct biochemical properties
... biosynthesis of taurine. In the present study, we compared the catalytic properties, inhibitor sensitivity and expression profiles of GADL1 and CSAD in brain tissue. In mouse and human brain we observed distinct patterns of expression of the PLP-dependent decarboxylases CSAD, GADL1 and glutamic acid ...
... biosynthesis of taurine. In the present study, we compared the catalytic properties, inhibitor sensitivity and expression profiles of GADL1 and CSAD in brain tissue. In mouse and human brain we observed distinct patterns of expression of the PLP-dependent decarboxylases CSAD, GADL1 and glutamic acid ...
Regulation of Phenylalanine and Tyrosine
... The chorismate mutase-prephenate de hydratase I aggregate and prephenate dehydratase II had molecular weights of 75000 and 30000, respectively. For P. aeruginosa strain P A 0 1 , the molecular weights of the corresponding enzymes determined by the same method were : prephenate dehydrogenase, 150000; ...
... The chorismate mutase-prephenate de hydratase I aggregate and prephenate dehydratase II had molecular weights of 75000 and 30000, respectively. For P. aeruginosa strain P A 0 1 , the molecular weights of the corresponding enzymes determined by the same method were : prephenate dehydrogenase, 150000; ...
Candida rugosa - Universität Stuttgart
... Transesterification of (±)-menthol using propionic acid anhydride and Candida rugosa lipase was performed in chloroform and water at different pressures (1, 10, 50, and 100 bar) to study the pressure dependence of enantioselectivity E. As a result, E significantly decreased with increasing pressure ...
... Transesterification of (±)-menthol using propionic acid anhydride and Candida rugosa lipase was performed in chloroform and water at different pressures (1, 10, 50, and 100 bar) to study the pressure dependence of enantioselectivity E. As a result, E significantly decreased with increasing pressure ...
Technical Brief
... The term kwashiorkor was introduced in the medical literature by Cicely Williams, a Jamaican physician working in what is now Ghana in an article published in the Lancet in 1935 1 (republished in 20032). It is derived from a word of the Krobo language from Ghana and refers to a child displaced from ...
... The term kwashiorkor was introduced in the medical literature by Cicely Williams, a Jamaican physician working in what is now Ghana in an article published in the Lancet in 1935 1 (republished in 20032). It is derived from a word of the Krobo language from Ghana and refers to a child displaced from ...
Cloning and sequencing of the kedarcidin biosynthetic
... chromophore were confirmed by high resolution mass spectrometry (Fig. S4, ESI†). For the enediyne form of the KED chromophore, high resolution electrospray ionization mass spectrometry (HRESIMS) yielded an [M + H]+ ion at m/z 1030.37338 for the enediyne form of the KED chromophore, consistent with i ...
... chromophore were confirmed by high resolution mass spectrometry (Fig. S4, ESI†). For the enediyne form of the KED chromophore, high resolution electrospray ionization mass spectrometry (HRESIMS) yielded an [M + H]+ ion at m/z 1030.37338 for the enediyne form of the KED chromophore, consistent with i ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... D’Angelo [1984] J. Infect. Dis. 1501480). strain challenge. It has similarly been found that persons recovering from a rickettsial infection may develop a solid ...
... D’Angelo [1984] J. Infect. Dis. 1501480). strain challenge. It has similarly been found that persons recovering from a rickettsial infection may develop a solid ...
NAGS - BMC Biochemistry
... Background: The efficient conversion of ammonia, a potent neurotoxin, into non-toxic metabolites was an essential adaptation that allowed animals to move from the aquatic to terrestrial biosphere. The urea cycle converts ammonia into urea in mammals, amphibians, turtles, snails, worms and many aquat ...
... Background: The efficient conversion of ammonia, a potent neurotoxin, into non-toxic metabolites was an essential adaptation that allowed animals to move from the aquatic to terrestrial biosphere. The urea cycle converts ammonia into urea in mammals, amphibians, turtles, snails, worms and many aquat ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.