Signatures of nitrogen limitation in the elemental composition of the
... GO:0008610). The species were chosen based on the amount of data available in the database, and A. thaliana was the only plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. P ...
... GO:0008610). The species were chosen based on the amount of data available in the database, and A. thaliana was the only plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. P ...
figure 1 - Proceedings of the Royal Society B
... GO:0008610). The species were chosen based on the amount of data available in the database, and A. thaliana was the only plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. P ...
... GO:0008610). The species were chosen based on the amount of data available in the database, and A. thaliana was the only plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. P ...
A novel multifunctional O-methyltransferase implicated in a dual
... catalyzing independently the methylation of caffeic acid and caffeoyl CoA. We therefore initiated the cloning of loblolly pine OMTs, with the initial focus on PCR cloning of CAOMT. By aligning deduced amino acid sequences of eight different plant CAOMTs, a highly conserved domain of about 170 aa res ...
... catalyzing independently the methylation of caffeic acid and caffeoyl CoA. We therefore initiated the cloning of loblolly pine OMTs, with the initial focus on PCR cloning of CAOMT. By aligning deduced amino acid sequences of eight different plant CAOMTs, a highly conserved domain of about 170 aa res ...
Designed sequences improve distant homology detection
... multiple sequence alignment. Designed sequences resembling naturally occurring sequences could potentially be used to improve sequence profile, leading to more powerful homology detection. Sequence design can also be used in studying protein function and evolution (9, 10), as it is often related to ...
... multiple sequence alignment. Designed sequences resembling naturally occurring sequences could potentially be used to improve sequence profile, leading to more powerful homology detection. Sequence design can also be used in studying protein function and evolution (9, 10), as it is often related to ...
Protein S-nitrosylation in photosynthetic organisms: A
... Stomata movements are regulated by abscisic acid (ABA) and this hormone is known to interact with NO in many processes and to stimulate its accumulation during stomata regulation [25]. In Arabidopsis mutants lacking the two NR isoforms, neither ABA-induced NO production nor ABA-induced stomata closu ...
... Stomata movements are regulated by abscisic acid (ABA) and this hormone is known to interact with NO in many processes and to stimulate its accumulation during stomata regulation [25]. In Arabidopsis mutants lacking the two NR isoforms, neither ABA-induced NO production nor ABA-induced stomata closu ...
Prokaryotic orthologues of mitochondrial alternative oxidase and plastid terminal oxidase
... The mitochondrial alternative oxidase (AOX) and the plastid terminal oxidase (PTOX) are two similar members of the membrane-bound diiron carboxylate group of proteins. AOX is a ubiquinol oxidase present in all higher plants, as well as some algae, fungi, and protists. It may serve to dampen reactive ...
... The mitochondrial alternative oxidase (AOX) and the plastid terminal oxidase (PTOX) are two similar members of the membrane-bound diiron carboxylate group of proteins. AOX is a ubiquinol oxidase present in all higher plants, as well as some algae, fungi, and protists. It may serve to dampen reactive ...
Lac Operon - Iowa State University
... 9. 14. A mutant E. coli strain, grown under conditions that normally induce the lac operon, produces high amounts of ß-galactosidase. What is a possible genotype of the cells? a) lacI+ lacP+ lacO+ lacZ- lacY+ lacA+ b) lacI+ lacP+ lacOc lacZ+ lacY+ lacA+ c) lacI- lacP+ lacO+ lacZ- lacY+ lacA+ d) lacI ...
... 9. 14. A mutant E. coli strain, grown under conditions that normally induce the lac operon, produces high amounts of ß-galactosidase. What is a possible genotype of the cells? a) lacI+ lacP+ lacO+ lacZ- lacY+ lacA+ b) lacI+ lacP+ lacOc lacZ+ lacY+ lacA+ c) lacI- lacP+ lacO+ lacZ- lacY+ lacA+ d) lacI ...
Protein Quality Control as a Strategy for Cellular Regulation
... protein degradation, referred to here as regulation and quality control. In regulation, ubiquitin-mediated degradation is used to effect regulatory changes in a specific protein’s levels and activity. Regulated degradation is keyed to physiological or developmental signals to allow selective loss of ...
... protein degradation, referred to here as regulation and quality control. In regulation, ubiquitin-mediated degradation is used to effect regulatory changes in a specific protein’s levels and activity. Regulated degradation is keyed to physiological or developmental signals to allow selective loss of ...
Table S1 Genes with similar expression patterns in Qing2
... Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss ...
... Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss ...
Imaging Single-mRNA Localization and Translation in Live Neurons
... for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in ne ...
... for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in ne ...
Plant development, auxin, and the subsystem
... fungi, and bacteria, including the cyanobacteria (Spaepen et al., 2007; Ross and Reid, 2010). A tryptophan-dependent pathway using indole-3-acetamide (IAM) as an intermediate to synthesize the most common auxin, indole-3-acetic acid (IAA), has been identified in methylobacteria (Hornschuh et al., 200 ...
... fungi, and bacteria, including the cyanobacteria (Spaepen et al., 2007; Ross and Reid, 2010). A tryptophan-dependent pathway using indole-3-acetamide (IAM) as an intermediate to synthesize the most common auxin, indole-3-acetic acid (IAA), has been identified in methylobacteria (Hornschuh et al., 200 ...
Hair follicle differentiation: expression, structure and
... et al. 1984; Fuchs et al. 1989; Roop et al. 1989; O'Guin et al. 1990). The keratin IF of epithelia form the largest group by far, comprising two families, each containing 10-15 proteins. The fundamental IF unit is composed of a heterodimer of one type I and one type II protein and approximately equa ...
... et al. 1984; Fuchs et al. 1989; Roop et al. 1989; O'Guin et al. 1990). The keratin IF of epithelia form the largest group by far, comprising two families, each containing 10-15 proteins. The fundamental IF unit is composed of a heterodimer of one type I and one type II protein and approximately equa ...
A Review of Issues of Dietary Protein Intake in Humans
... to weight loss and diabetic glycemic control, the second being the long-standing interest of those in the health, fitness, and body building fraternity with increased protein intake for perceived benefits in muscle development. A comprehensive study of dietary protein in weight loss and glucose home ...
... to weight loss and diabetic glycemic control, the second being the long-standing interest of those in the health, fitness, and body building fraternity with increased protein intake for perceived benefits in muscle development. A comprehensive study of dietary protein in weight loss and glucose home ...
Chemical synthesis of proteins
... bonds, peptides containing either aldehyde or Nterminal aminooxyacetyl groups can be ligated to form oxime bonds71,72 (Fig. 3c). An aldehyde-containing peptide can also be ligated to a peptide containing a weak nucleophilic base, such as hydrazide, N-terminal Cys or N-terminal Thr, to form hydrazone ...
... bonds, peptides containing either aldehyde or Nterminal aminooxyacetyl groups can be ligated to form oxime bonds71,72 (Fig. 3c). An aldehyde-containing peptide can also be ligated to a peptide containing a weak nucleophilic base, such as hydrazide, N-terminal Cys or N-terminal Thr, to form hydrazone ...
Calcium Homeostasis in Plants: Role of Calcium Binding Proteins in
... ACA2p in membrane fractions and this was confirmed by cytological visualization of an ACA2p tagged with a C-terminal green fluorescent protein (Harper et ai, 1998; Hong et ai, 1999). (ii) Ca 2 +/H+ antiporters. They are different from Ca 2+-ATPases as they do not require ATP and are not sensitive to ...
... ACA2p in membrane fractions and this was confirmed by cytological visualization of an ACA2p tagged with a C-terminal green fluorescent protein (Harper et ai, 1998; Hong et ai, 1999). (ii) Ca 2 +/H+ antiporters. They are different from Ca 2+-ATPases as they do not require ATP and are not sensitive to ...
The presence of monoglucosylated N196
... protein–protein interaction, immune response and responses to pathogens [2–4]. More than half of all eukaryotic proteins have been reported to be glycosylated, and N-glycan is estimated to be present in 90 % of these proteins [5]. The crystal structures and NMR studies of glycoproteins indicate that ...
... protein–protein interaction, immune response and responses to pathogens [2–4]. More than half of all eukaryotic proteins have been reported to be glycosylated, and N-glycan is estimated to be present in 90 % of these proteins [5]. The crystal structures and NMR studies of glycoproteins indicate that ...
Full-Text PDF
... results were interpreted in the context of the docking results. The results suggest that an extended surface along the active site cleft is involved in binding of a protein substrate. Furthermore, it appears that a number of hydrophobic and aromatic residues are important for interacting with Z-QG, ...
... results were interpreted in the context of the docking results. The results suggest that an extended surface along the active site cleft is involved in binding of a protein substrate. Furthermore, it appears that a number of hydrophobic and aromatic residues are important for interacting with Z-QG, ...
Characterizing the Grape Transcriptome
... Ontology categories. A simple structured vocabulary, with modules for plant genotype, plant development, and stress, was developed to describe the relationship between individual expressed sequence tags and cDNA libraries; the resulting vocabulary provides query terms to facilitate data mining withi ...
... Ontology categories. A simple structured vocabulary, with modules for plant genotype, plant development, and stress, was developed to describe the relationship between individual expressed sequence tags and cDNA libraries; the resulting vocabulary provides query terms to facilitate data mining withi ...
Fulltext - Jultika
... NRBF-1 resides in mitochondria in cultured HeLa cells. The recombinant NRBF-1 is enzymatically active, reducing 2E-enoyl-CoAs to acyl-CoAs in an NADPH-dependent manner. Altogether, our data showed that NRBF-1 is a mitochondrial 2-enoyl-CoA reductase/2-enoyl thioester reductase (MECR/ETR1), the human ...
... NRBF-1 resides in mitochondria in cultured HeLa cells. The recombinant NRBF-1 is enzymatically active, reducing 2E-enoyl-CoAs to acyl-CoAs in an NADPH-dependent manner. Altogether, our data showed that NRBF-1 is a mitochondrial 2-enoyl-CoA reductase/2-enoyl thioester reductase (MECR/ETR1), the human ...
Determination of De Novo Synthesized Amino Acids in Cellular
... protein were found for alanine and proline, whereas the amount of 13C label in glycine and glutamate was about twoto three-fold greater in the PCA than in the protein fraction. However, the higher enrichment of glutamate C4 vs C3 observed in the cytosolic fraction was also found in the cellular prot ...
... protein were found for alanine and proline, whereas the amount of 13C label in glycine and glutamate was about twoto three-fold greater in the PCA than in the protein fraction. However, the higher enrichment of glutamate C4 vs C3 observed in the cytosolic fraction was also found in the cellular prot ...
Monitoring Growth of Beer Brewing Strains of
... order to keep cells in suspension. The size and propensity of yeast cells to clump together necessitates the use of orbital shaking to maintain cells in suspension. With the strains we examined, a slow setting with a short-amplitude (1 mm) selected (559 cpm) provided adequate agitation to keep the y ...
... order to keep cells in suspension. The size and propensity of yeast cells to clump together necessitates the use of orbital shaking to maintain cells in suspension. With the strains we examined, a slow setting with a short-amplitude (1 mm) selected (559 cpm) provided adequate agitation to keep the y ...