Protein structural class prediction using predicted secondary
... Chapter 5 presents the results of the thesis work. This chapter includes the comparison of performance of various feature sets developed for this thesis. The comparison of performance of the best performing feature sets with some major published work is also presented in this chapter. Chapter 6 conc ...
... Chapter 5 presents the results of the thesis work. This chapter includes the comparison of performance of various feature sets developed for this thesis. The comparison of performance of the best performing feature sets with some major published work is also presented in this chapter. Chapter 6 conc ...
Simulation of Enzyme Reaction - diss.fu
... The electrostatic models used today consider these effects, however sometimes only implicitly. In standard empirical force fields used for Molecular Dynamics or Monte Carlo simulations all atoms of a molecular system are represented in detail. All electrostatic interactions between the corresponding ...
... The electrostatic models used today consider these effects, however sometimes only implicitly. In standard empirical force fields used for Molecular Dynamics or Monte Carlo simulations all atoms of a molecular system are represented in detail. All electrostatic interactions between the corresponding ...
Refining the Definition of Plant Mitochondrial
... presequences for plant mitochondrial proteins. Later analysis of 58 experimentally proven plant mitochondrial presequences deposited in the Swiss-Prot database revealed two major classes containing an Arg residue at positions 22 and 23 and one class without any conserved Arg residues (Zhang et al., ...
... presequences for plant mitochondrial proteins. Later analysis of 58 experimentally proven plant mitochondrial presequences deposited in the Swiss-Prot database revealed two major classes containing an Arg residue at positions 22 and 23 and one class without any conserved Arg residues (Zhang et al., ...
Site-Directed Mutagenesis of Predicted Active Site Residues in
... loss of enzyme activity (Table 3). The severe loss of activity in these mutants is not due to the inability of transfectants to express GCP II protein, as determined by immunoblot analysis (examples shown in Fig. 2A). However, reduced expression was observed for some of these mutants (Table 3). The ...
... loss of enzyme activity (Table 3). The severe loss of activity in these mutants is not due to the inability of transfectants to express GCP II protein, as determined by immunoblot analysis (examples shown in Fig. 2A). However, reduced expression was observed for some of these mutants (Table 3). The ...
AtMYB93 is a novel negative regulator of lateral root
... likelihood algorithm in SeaView on default settings, with 1000 bootstrap replicates. Similar ...
... likelihood algorithm in SeaView on default settings, with 1000 bootstrap replicates. Similar ...
The p101 subunit of PI3Kγ restores activation by Gβ mutants
... Class I PI3Ks (phosphoinositide 3-kinases) are secondmessenger-generating enzymes, which transform extracellular signals into the principle product PtdIns(3,4,5)P3 in order to control a plethora of fundamental cellular responses, including proliferation, differentiation, growth and chemotaxis [1–8]. ...
... Class I PI3Ks (phosphoinositide 3-kinases) are secondmessenger-generating enzymes, which transform extracellular signals into the principle product PtdIns(3,4,5)P3 in order to control a plethora of fundamental cellular responses, including proliferation, differentiation, growth and chemotaxis [1–8]. ...
Biochemical Engineering Prof. Dr. Rintu Banerjee Department of
... Now this amino acids are playing a very important role as far as the mechanism the cell functionality is concerned and that is the reason this amino acids has been classified or symbolized with either three letter digit or with one letter digit say for example, now glycine if we considered glycine a ...
... Now this amino acids are playing a very important role as far as the mechanism the cell functionality is concerned and that is the reason this amino acids has been classified or symbolized with either three letter digit or with one letter digit say for example, now glycine if we considered glycine a ...
Materials - HAL
... at their N-terminal part which binds a number of proteins (EBP 50/NHE3-RF, RhoGDI,…) some of which are transmembrane proteins such as CD44, CD43, ICAM-2, ICAM-3. Furthermore, ERM proteins can self-associate in an intra- or inter-molecular manner through homotypic and heterotypic interaction between ...
... at their N-terminal part which binds a number of proteins (EBP 50/NHE3-RF, RhoGDI,…) some of which are transmembrane proteins such as CD44, CD43, ICAM-2, ICAM-3. Furthermore, ERM proteins can self-associate in an intra- or inter-molecular manner through homotypic and heterotypic interaction between ...
A defined subset of adenylyl cyclases is regulated by bicarbonate ion.
... determine whether HCO3 - up regulated cyaB1595-859 specific activity by increasing homodimer formation we examined the ratio of the HCO3- and Cl- specific activities as a function of protein concentration. Interestingly, this ratio remained constant over the range of protein concentrations tested in ...
... determine whether HCO3 - up regulated cyaB1595-859 specific activity by increasing homodimer formation we examined the ratio of the HCO3- and Cl- specific activities as a function of protein concentration. Interestingly, this ratio remained constant over the range of protein concentrations tested in ...
The subunit of voltage sensitive Ca 2+ channels is a single
... An Xba (2675) cDNA fragment of the c~2 subunit, was filled-in, cut with DraI (2160), isolated and subcloned into the bacterial expression vector pGEX-KG [24]. The GST/~2 fusion protein, induced by IPTG (0.1 M) in TG1 cells (Escherichia coli), was affinity purified on GSTagarose according to protocol ...
... An Xba (2675) cDNA fragment of the c~2 subunit, was filled-in, cut with DraI (2160), isolated and subcloned into the bacterial expression vector pGEX-KG [24]. The GST/~2 fusion protein, induced by IPTG (0.1 M) in TG1 cells (Escherichia coli), was affinity purified on GSTagarose according to protocol ...
Surface complementarity of buried protein residues
... constituting hydrophobic cores carry high informational content3. This is borne out by the fact that site-directed mutagenesis of these residues are generally destabilizing4,5 for the protein. Further sequence and structure comparison studies between naturally occurring homologous proteins show buri ...
... constituting hydrophobic cores carry high informational content3. This is borne out by the fact that site-directed mutagenesis of these residues are generally destabilizing4,5 for the protein. Further sequence and structure comparison studies between naturally occurring homologous proteins show buri ...
Increased and controlled expression of the Rickettsia
... identified in immunoblots of SDS-solubilized membranes of R. prowaxekii and translocase-expressing E. coli using antibodies against a synthetic peptide corresponding to the carboxyl-terminal 17 amino acids of the deduced protein (Plano & Winkler, 1991). These antibodies also bound to inside-out memb ...
... identified in immunoblots of SDS-solubilized membranes of R. prowaxekii and translocase-expressing E. coli using antibodies against a synthetic peptide corresponding to the carboxyl-terminal 17 amino acids of the deduced protein (Plano & Winkler, 1991). These antibodies also bound to inside-out memb ...
The HSP90-SGT1 Chaperone Complex for NLR
... system components could be lethal. The short list of signaling components that could be isolated in the genetic screen would thus provide only a limited number of targets for disruption by pathogen attack. The conciseness of this system may in fact be one of its key selfprotective mechanisms, and al ...
... system components could be lethal. The short list of signaling components that could be isolated in the genetic screen would thus provide only a limited number of targets for disruption by pathogen attack. The conciseness of this system may in fact be one of its key selfprotective mechanisms, and al ...
Introduction to Carbohydrates
... The concentration of free amino acids in the extracellular fluids is significantly lower than that within the cells of the body. This concentration gradient is maintained because active transport systems, driven by the hydrolysis of ATP, are required for movement of amino acids from the extracel ...
... The concentration of free amino acids in the extracellular fluids is significantly lower than that within the cells of the body. This concentration gradient is maintained because active transport systems, driven by the hydrolysis of ATP, are required for movement of amino acids from the extracel ...
Roles for Regulator of G Protein Signaling Proteins in Synaptic
... This interpretation is strengthened by in vitro evidence showing RGS2-mediated facilitation of CaV2.1 calcium channels, which are also inhibited by Gbg subunits (Mark et al., 2000). RGS2 also has reported roles in postsynaptic spines within the hippocampus in the context of long-term synaptic plasti ...
... This interpretation is strengthened by in vitro evidence showing RGS2-mediated facilitation of CaV2.1 calcium channels, which are also inhibited by Gbg subunits (Mark et al., 2000). RGS2 also has reported roles in postsynaptic spines within the hippocampus in the context of long-term synaptic plasti ...
pdf file - John Innes Centre
... where, surrounded by a peribacteroid membrane (PBM) of plant origin (which controls the nutrient transfer between the symbionts), they differentiate into nitrogen-fixing symbiosomes (i.e., they are able to reduce atmospheric dinitrogen [N2] to ammonia [NH3]) through the activation of the nitrogenase ...
... where, surrounded by a peribacteroid membrane (PBM) of plant origin (which controls the nutrient transfer between the symbionts), they differentiate into nitrogen-fixing symbiosomes (i.e., they are able to reduce atmospheric dinitrogen [N2] to ammonia [NH3]) through the activation of the nitrogenase ...
Characterization of Vincristine Transport by the Mr 190,000
... of this differential sensitivity is unknown. Finally, in addition to chemotherapeutic agents, MRP also confers resistance to certain an timonial and arsenical oxyanions, a property not associated with overexpression of P-glycoprotein (9, 12). Thus, although the crossresistance profiles of these two ...
... of this differential sensitivity is unknown. Finally, in addition to chemotherapeutic agents, MRP also confers resistance to certain an timonial and arsenical oxyanions, a property not associated with overexpression of P-glycoprotein (9, 12). Thus, although the crossresistance profiles of these two ...
Full-Text PDF
... esterified triacylglycerols carried by lipoproteins, or in a non-esterified form bound to albumin. The plasma concentration of HSA is around 0.6 mM and the molecule can carry at least six molecules of fatty acids. Under normal physiological conditions, HSA carries approximately 0.1–2 moles of fatty ...
... esterified triacylglycerols carried by lipoproteins, or in a non-esterified form bound to albumin. The plasma concentration of HSA is around 0.6 mM and the molecule can carry at least six molecules of fatty acids. Under normal physiological conditions, HSA carries approximately 0.1–2 moles of fatty ...
Fluorescent Reporter Proteins for the Tonoplast
... yellow fluorescent protein (YFP). We also produced soluble reporters consisting of the monomeric red fluorescent protein (RFP) and either the C-terminal vacuolar sorting signal of phaseolin or the sequence-specific sorting signal of proricin. In transgenic Arabidopsis leaves, mature roots, and root ...
... yellow fluorescent protein (YFP). We also produced soluble reporters consisting of the monomeric red fluorescent protein (RFP) and either the C-terminal vacuolar sorting signal of phaseolin or the sequence-specific sorting signal of proricin. In transgenic Arabidopsis leaves, mature roots, and root ...
Marginally hydrophobic transmembrane helices shaping membrane protein folding
... acyl chain allowing it to pack tightly, whereas unsaturated fatty acids contain kinks resulting in increased fluidity. In addition, incorporation of rigid steroidic lipids, such as cholesterol, confer stability (16) . Membrane fluidity is also dependent on temperature and some organisms can adjust t ...
... acyl chain allowing it to pack tightly, whereas unsaturated fatty acids contain kinks resulting in increased fluidity. In addition, incorporation of rigid steroidic lipids, such as cholesterol, confer stability (16) . Membrane fluidity is also dependent on temperature and some organisms can adjust t ...
N-Glycans
... on misfolded glycoproteins and escorts them via retrotranslocation into the cytoplasm for degradation. The removal of the first glucose (and therefore all glucose) can be blocked by castanospermin. For most glycoproteins, additional mannose residues are removed in the cis compartment of the Golgi un ...
... on misfolded glycoproteins and escorts them via retrotranslocation into the cytoplasm for degradation. The removal of the first glucose (and therefore all glucose) can be blocked by castanospermin. For most glycoproteins, additional mannose residues are removed in the cis compartment of the Golgi un ...
Effect of RNAi down-regulation of three lysine-deficient
... within two weeks. (C) Sorghum genotype P898012 plantlets shooting and rooting on regime J regeneration medium. These plantlets resulted in fertile F0 plants. (D) Mature sorghum head from tissue culture plants. ...
... within two weeks. (C) Sorghum genotype P898012 plantlets shooting and rooting on regime J regeneration medium. These plantlets resulted in fertile F0 plants. (D) Mature sorghum head from tissue culture plants. ...
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library
... consisted of 4.3 × 103 clones, has an average insert length of 0.9 kb ranging from 200 to 1500 kb (Figure 2). After construction of cDNA libraries, 1536 clones were randomly selected and sequenced from prenatal stage library; 1536 clones were randomly selected and sequenced from postnatal stage libr ...
... consisted of 4.3 × 103 clones, has an average insert length of 0.9 kb ranging from 200 to 1500 kb (Figure 2). After construction of cDNA libraries, 1536 clones were randomly selected and sequenced from prenatal stage library; 1536 clones were randomly selected and sequenced from postnatal stage libr ...
the structure and function of cartilage proteoglycans
... survives at all and is not also replaced by bone. At first sight, articular cartilage seems ill-equipped to deal with the constant mechanical insults to which it is exposed during normal joint function. It has no vascular system to provide nutrition and is only sparsely populated with cells, which a ...
... survives at all and is not also replaced by bone. At first sight, articular cartilage seems ill-equipped to deal with the constant mechanical insults to which it is exposed during normal joint function. It has no vascular system to provide nutrition and is only sparsely populated with cells, which a ...