the structure and function of cartilage proteoglycans
... survives at all and is not also replaced by bone. At first sight, articular cartilage seems ill-equipped to deal with the constant mechanical insults to which it is exposed during normal joint function. It has no vascular system to provide nutrition and is only sparsely populated with cells, which a ...
... survives at all and is not also replaced by bone. At first sight, articular cartilage seems ill-equipped to deal with the constant mechanical insults to which it is exposed during normal joint function. It has no vascular system to provide nutrition and is only sparsely populated with cells, which a ...
Peroxisomes: family of versatile organelles
... Towards the molecular mechanism of the integration of peroxisomal membrane proteins Targeting and insertion of peroxisomal membrane proteins: ER trafficking versus direct delivery to peroxisomes Multiple paths to peroxisomes: Mechanism of peroxisome maintenance in mammals De novo peroxisome biogenes ...
... Towards the molecular mechanism of the integration of peroxisomal membrane proteins Targeting and insertion of peroxisomal membrane proteins: ER trafficking versus direct delivery to peroxisomes Multiple paths to peroxisomes: Mechanism of peroxisome maintenance in mammals De novo peroxisome biogenes ...
Functional Roles Of Lipids In membranes - IJS
... glycerol-based glycolipids are major membrane-forming components in many Grampositive bacteria and in the membranes of plants while Gram-negative bacteria utilize a glucosamine-based phospholipid (Lipid A) as a major structural component of the outer membrane. Additional diversity results in the var ...
... glycerol-based glycolipids are major membrane-forming components in many Grampositive bacteria and in the membranes of plants while Gram-negative bacteria utilize a glucosamine-based phospholipid (Lipid A) as a major structural component of the outer membrane. Additional diversity results in the var ...
Utilization of organophosphate:phosphate antiporter for isotope
... genes were fused to obtain a C-terminal FLAG-tag. Beside the expression based on a multi-copy plasmid, a Ptac controlled expression cassette of each transporter gene was also integrated as single copy into the chromosome of E. coli. Therefore, downstream of the recombinant biosynthesis gene, a chlor ...
... genes were fused to obtain a C-terminal FLAG-tag. Beside the expression based on a multi-copy plasmid, a Ptac controlled expression cassette of each transporter gene was also integrated as single copy into the chromosome of E. coli. Therefore, downstream of the recombinant biosynthesis gene, a chlor ...
Forespore membrane assembly in yeast
... Because the S. pombe spo3 mutant produces no spores, SpSpo3 must be essential for FSM formation (Nakamura et al., 2001). In fact, the FSM is aberrant in this mutant, as revealed by another FSM marker, GFP-Psy1. Spspo3+ is transcribed when cells are induced to undergo meiosis. SpSpo3 shows no apparen ...
... Because the S. pombe spo3 mutant produces no spores, SpSpo3 must be essential for FSM formation (Nakamura et al., 2001). In fact, the FSM is aberrant in this mutant, as revealed by another FSM marker, GFP-Psy1. Spspo3+ is transcribed when cells are induced to undergo meiosis. SpSpo3 shows no apparen ...
Marine
... Effect of fluid shear A defined laminar shear environment was obtained using a rotating cylinder apparatus (van Duuren 1968) consisting of two vertically aligned concentric acrylic cylinders separated by a 9-mm gap. The fixed inner cylinder was 8 em in diameter and 15 em high. The outer cylinder rot ...
... Effect of fluid shear A defined laminar shear environment was obtained using a rotating cylinder apparatus (van Duuren 1968) consisting of two vertically aligned concentric acrylic cylinders separated by a 9-mm gap. The fixed inner cylinder was 8 em in diameter and 15 em high. The outer cylinder rot ...
Supplementary information for Ronshaugen, McGinnis
... used to design primers for 3’ and 5’ RACE (rapid amplification of cDNA ends) using the Marathon cDNA amplification system (Clontech). Primers were constructed in the 5’ and 3’ UTR of the RACE products and used to amplify full-length cDNA’s from multiple independent poly-T primed reverse transcriptas ...
... used to design primers for 3’ and 5’ RACE (rapid amplification of cDNA ends) using the Marathon cDNA amplification system (Clontech). Primers were constructed in the 5’ and 3’ UTR of the RACE products and used to amplify full-length cDNA’s from multiple independent poly-T primed reverse transcriptas ...
Homologous and heterologous reconstitution of Golgi to chloroplast
... carboxylase (pSSU) and pre-sequence deletion constructs was used to map the transit peptide domain to the region between the signal peptidase cleavage site and the membrane anchor sequence. The pre-sequence region C-terminal to the hydrophobic membrane anchor that remains on the cytoplasmic membrane ...
... carboxylase (pSSU) and pre-sequence deletion constructs was used to map the transit peptide domain to the region between the signal peptidase cleavage site and the membrane anchor sequence. The pre-sequence region C-terminal to the hydrophobic membrane anchor that remains on the cytoplasmic membrane ...
Statistical potential-based amino acid similarity
... derived from a representative set of 3156 protein structures. This representative set is selected by the PDBREPRDB server with the default parameter set45 that excludes proteins that have 30% or greater sequence identity with the other members in the set. By the following methods described by Skolni ...
... derived from a representative set of 3156 protein structures. This representative set is selected by the PDBREPRDB server with the default parameter set45 that excludes proteins that have 30% or greater sequence identity with the other members in the set. By the following methods described by Skolni ...
2013_BJ_SCFBio_Website
... http://www.riethoven.org/BioInformer/newsletter/archives/2/procrustes.html ...
... http://www.riethoven.org/BioInformer/newsletter/archives/2/procrustes.html ...
Gene Section TYR (tyrosinase (oculocutaneous albinism IA)) Atlas of Genetics and Cytogenetics
... Schematic of Tyrosinase Polypeptide adapted from Mashima 1994. SP (signal peptide), EGF (Epidermal growth factor)-like domain, CuA and CuB (Copper binding domains) and TM (transmembrane domain). ...
... Schematic of Tyrosinase Polypeptide adapted from Mashima 1994. SP (signal peptide), EGF (Epidermal growth factor)-like domain, CuA and CuB (Copper binding domains) and TM (transmembrane domain). ...
Homologous and heterologous reconstitution of Golgi to chloroplast
... carboxylase (pSSU) and pre-sequence deletion constructs was used to map the transit peptide domain to the region between the signal peptidase cleavage site and the membrane anchor sequence. The pre-sequence region C-terminal to the hydrophobic membrane anchor that remains on the cytoplasmic membrane ...
... carboxylase (pSSU) and pre-sequence deletion constructs was used to map the transit peptide domain to the region between the signal peptidase cleavage site and the membrane anchor sequence. The pre-sequence region C-terminal to the hydrophobic membrane anchor that remains on the cytoplasmic membrane ...
Strategy for Nonenveloped Virus Entry
... measured the extent of hemolysis. None of the particles induced hemolysis upon incubation on ice (Fig. 1B), but, when incubated with RBCs at 32°C, T3D ISVPs lysed essentially all of the cells (Fig. 1B). In contrast, T3D virions and cores were inactive for hemolysis at these and all conditions tested ...
... measured the extent of hemolysis. None of the particles induced hemolysis upon incubation on ice (Fig. 1B), but, when incubated with RBCs at 32°C, T3D ISVPs lysed essentially all of the cells (Fig. 1B). In contrast, T3D virions and cores were inactive for hemolysis at these and all conditions tested ...
Increased Leaf Angle1, a Raf-Like MAPKKK That
... of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714) (Figure 3A), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of IL ...
... of 564 amino acids and a molecular mass of 63 kD. According to the Pfam database, the deduced ILA1 has an N-terminal ACT domain (PF01842) and a C-terminal kinase domain (PF07714) (Figure 3A), which likely confer a protein regulatory feature and kinase activity, respectively. Sequence alignment of IL ...
Molecular and General Genetics
... Cloning of the phoM gene Since the constitutive synthesis of alkaline phosphatase in phoR mutants is dependent on a phoM + allele, we attempted to clone the phoM gene by selecting for hybrid plasmids conferring constitutive synthesis of alkaline phosphatase to a phoR phoM double mutant. Purified chr ...
... Cloning of the phoM gene Since the constitutive synthesis of alkaline phosphatase in phoR mutants is dependent on a phoM + allele, we attempted to clone the phoM gene by selecting for hybrid plasmids conferring constitutive synthesis of alkaline phosphatase to a phoR phoM double mutant. Purified chr ...
Novel Ubiquitin Fusion Proteins: Ribosomal Protein
... present in B. natans polyubiquitin genes and to the ubiquitin moieties of the S27a and L40 fusion proteins. In contrast, the ubiquitin extension encoded by actin-3 was more divergent at the protein level than is typical of ubiquitin (the cDNA encoding this gene was slightly truncated at its 50 end). ...
... present in B. natans polyubiquitin genes and to the ubiquitin moieties of the S27a and L40 fusion proteins. In contrast, the ubiquitin extension encoded by actin-3 was more divergent at the protein level than is typical of ubiquitin (the cDNA encoding this gene was slightly truncated at its 50 end). ...
A New Subunit of Cytochrome b6f Complex Undergoes Reversible
... Sequence and Topology of the PETO Gene Product—We have previously identified a nucleus-encoded polypeptide of 19-kDa apparent molecular mass that was absent from the thylakoid membranes of cytb6f mutants (21). To avoid its copurification with some ATP synthase subunits that display a similar apparen ...
... Sequence and Topology of the PETO Gene Product—We have previously identified a nucleus-encoded polypeptide of 19-kDa apparent molecular mass that was absent from the thylakoid membranes of cytb6f mutants (21). To avoid its copurification with some ATP synthase subunits that display a similar apparen ...
Two Genes with Similarity to Bacterial Response Regulators Are
... Very little is known about the mechanisms underlying cytokinin perception and signaling. One likely cytokinin signaling element recently was identified by its ability, when overexpressed, to confer cytokinin-independent growth to Arabidopsis cells in culture (Kakimoto, 1996). This CKI1 (for cytokini ...
... Very little is known about the mechanisms underlying cytokinin perception and signaling. One likely cytokinin signaling element recently was identified by its ability, when overexpressed, to confer cytokinin-independent growth to Arabidopsis cells in culture (Kakimoto, 1996). This CKI1 (for cytokini ...
Protein synthesis meets ABC ATPases: new roles for Rli1/ABCE1
... catalysed by several translation factors. The fundamental reactions of protein synthesis, such as mRNA decoding, peptide bond formation and tRNA translocation, follow the same basic principles in prokaryotes and eukaryotes. However, some steps are quite different and require a larger set of factors ...
... catalysed by several translation factors. The fundamental reactions of protein synthesis, such as mRNA decoding, peptide bond formation and tRNA translocation, follow the same basic principles in prokaryotes and eukaryotes. However, some steps are quite different and require a larger set of factors ...
Characterisation of Milk Proteins in Confectionery Products
... be distinguished from one made with a dry milk powder on the basis of a more even distribution of protein and a high level of association between milk protein and other ingredients. Caramel was seen to be composed of an amorphous sugar support matrix containing numerous fat globules. Milk protein wa ...
... be distinguished from one made with a dry milk powder on the basis of a more even distribution of protein and a high level of association between milk protein and other ingredients. Caramel was seen to be composed of an amorphous sugar support matrix containing numerous fat globules. Milk protein wa ...
Metabolic rate depression in animals
... becomes intermittent, and kidney filtration rate is reduced. Organisms do not eat so the energetic costs of digestion, nutrient absorption, and peristalsis are eliminated. A substantial part of total energy savings comes from the suppression of these physiological activities. Metabolic rate is also i ...
... becomes intermittent, and kidney filtration rate is reduced. Organisms do not eat so the energetic costs of digestion, nutrient absorption, and peristalsis are eliminated. A substantial part of total energy savings comes from the suppression of these physiological activities. Metabolic rate is also i ...
Evolution of an ancient protein function involved in
... such as the kinesin-3 family member KHC-73, and the Partner of Inscuteable protein (Pins in insects, LGN in vertebrates). In neuroblasts, the complex is localized relative to the position of adjacent cells by the interaction of a transmembrane receptor – which receives local extracellular signals – ...
... such as the kinesin-3 family member KHC-73, and the Partner of Inscuteable protein (Pins in insects, LGN in vertebrates). In neuroblasts, the complex is localized relative to the position of adjacent cells by the interaction of a transmembrane receptor – which receives local extracellular signals – ...
intolerance to lactose and other dietary sugars
... FIG. 2. Diagram showing the likely mechanism of the cis-acting polymorphism in determining lactase expression in persistent and nonpersistent adults. A developmentally regulated DNA binding protein may interact with a cis-element present on one allele and not the other (due to sequence differences w ...
... FIG. 2. Diagram showing the likely mechanism of the cis-acting polymorphism in determining lactase expression in persistent and nonpersistent adults. A developmentally regulated DNA binding protein may interact with a cis-element present on one allele and not the other (due to sequence differences w ...
Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus
... containing the $ 2 ~ ' ~ suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the S U + ~and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain . S ...
... containing the $ 2 ~ ' ~ suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the S U + ~and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain . S ...
Characterisation of the novel proteins expressed in corn line 1507
... B. thuringiensis are a diverse group of Gram-positive, spore-forming bacteria that were first isolated in 1901, and have proven to be a rich source of insecticidal proteins. Intensive research has identified a growing family of Bt proteins with different insecticidal specificities, including to cole ...
... B. thuringiensis are a diverse group of Gram-positive, spore-forming bacteria that were first isolated in 1901, and have proven to be a rich source of insecticidal proteins. Intensive research has identified a growing family of Bt proteins with different insecticidal specificities, including to cole ...