Package `PPInfer`
... Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivated the development of a variety of experimental methods for the identification of ...
... Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivated the development of a variety of experimental methods for the identification of ...
In Silico Prediction of the Peroxisomal Proteome in Fungi, Plants
... Pex7p.17 After binding of their cargo proteins, these receptors are thought to interact with a docking complex consisting of Pex13p and Pex14p, where both pathways seem to merge18,19 and then shuttle back into the cytosol for the next round of targeting. At present, several peroxins involved in the ...
... Pex7p.17 After binding of their cargo proteins, these receptors are thought to interact with a docking complex consisting of Pex13p and Pex14p, where both pathways seem to merge18,19 and then shuttle back into the cytosol for the next round of targeting. At present, several peroxins involved in the ...
results and discussion discussion
... The growth rate of clones and control in LB containing ampicillin medium was different i.e. clone 5B2 and 6S2 growth rate was double as compared to the clone 6B4 and control E. coli/pUC19 (Fig. 4.4.2). Similarly, growth rate of the clones and control with the different salt stress condition such as ...
... The growth rate of clones and control in LB containing ampicillin medium was different i.e. clone 5B2 and 6S2 growth rate was double as compared to the clone 6B4 and control E. coli/pUC19 (Fig. 4.4.2). Similarly, growth rate of the clones and control with the different salt stress condition such as ...
pdf
... dve-1 knockdown by RNAi directed to the gene’s 30 UTR (Figure S1), but it is unable to rescue the lethality associated with a null mutation (data not shown). Heterozygous animals with a deletion allele in dve1(tm259)X were obtained from the National Bioresource Project for C. elegans (Tokyo). The 98 ...
... dve-1 knockdown by RNAi directed to the gene’s 30 UTR (Figure S1), but it is unable to rescue the lethality associated with a null mutation (data not shown). Heterozygous animals with a deletion allele in dve1(tm259)X were obtained from the National Bioresource Project for C. elegans (Tokyo). The 98 ...
The Organellar Genome and Metabolic Potential
... of genes and proteins have been lost, gained from various sources, or retargeted to other organelles, thus shaping the huge diversity of current mitochondria and their homologs in various species (Gabaldón and Huynen 2004). Within the evolutionary ‘‘gap’’ between species with genome-containing mito ...
... of genes and proteins have been lost, gained from various sources, or retargeted to other organelles, thus shaping the huge diversity of current mitochondria and their homologs in various species (Gabaldón and Huynen 2004). Within the evolutionary ‘‘gap’’ between species with genome-containing mito ...
Fulltext PDF - Indian Academy of Sciences
... Entamoeba histolytica and Giardia lamblia are microaerophilic protists, which have long been considered models of ancient pre-mitochondriate eukaryotes. As transitional eukaryotes, amoebae and giardia appeared to lack organelles of higher eukaryotes and to depend upon energy metabolism appropriate f ...
... Entamoeba histolytica and Giardia lamblia are microaerophilic protists, which have long been considered models of ancient pre-mitochondriate eukaryotes. As transitional eukaryotes, amoebae and giardia appeared to lack organelles of higher eukaryotes and to depend upon energy metabolism appropriate f ...
The functional cobalamin (vitamin B12)–intrinsic factor receptor is a
... canine I-GS shows highly significant linkage to the same locus.26 AMN was initially identified by random mutagenesis27 as a protein essential for amnion and primitive streak formation in mice. AMN is a transmembrane 45- to 50-kDa protein of polarized epithelia.24,27 The function of AMN is otherwise ...
... canine I-GS shows highly significant linkage to the same locus.26 AMN was initially identified by random mutagenesis27 as a protein essential for amnion and primitive streak formation in mice. AMN is a transmembrane 45- to 50-kDa protein of polarized epithelia.24,27 The function of AMN is otherwise ...
Evolution of Function in Protein Superfamilies, from a Structural
... The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as de®ned by the CATH protein structure classi®cation, by way of the Enzyme Commiss ...
... The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as de®ned by the CATH protein structure classi®cation, by way of the Enzyme Commiss ...
Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print
... event and transforms it into a measurable signal. The molecular biosensor can be distinguished from a chemosensor by the nature of the receptor, which is typically a biomacromolecule such as a nucleic acid (DNA or RNA), or a protein (often an enzyme or an antibody). Fluorescent molecular biosensors ...
... event and transforms it into a measurable signal. The molecular biosensor can be distinguished from a chemosensor by the nature of the receptor, which is typically a biomacromolecule such as a nucleic acid (DNA or RNA), or a protein (often an enzyme or an antibody). Fluorescent molecular biosensors ...
Review Non-ribosomal peptide synthetases: Identifying the cryptic
... in an uncharacterized A-domain, then the probability of their substrates being the same is high (Stachelhaus et al. 1999). This comparative analysis is used by many NRPS prediction tools like the SBS-PKS, PKS/NRPS and NP.searcher (table 1) to predict the approximate product chemistry of novel NRPS c ...
... in an uncharacterized A-domain, then the probability of their substrates being the same is high (Stachelhaus et al. 1999). This comparative analysis is used by many NRPS prediction tools like the SBS-PKS, PKS/NRPS and NP.searcher (table 1) to predict the approximate product chemistry of novel NRPS c ...
PAI-1 - Trace: Tennessee Research and Creative Exchange
... and assist in inflammatory response. From immobilized metal affinity chromatography (IMAC) purification it was known that recombinant PAI-1 binds metals, and the role of metals in PAI-1 is relevant because metals have been demonstrated to play a role in the severity of inflammatory response, tumor p ...
... and assist in inflammatory response. From immobilized metal affinity chromatography (IMAC) purification it was known that recombinant PAI-1 binds metals, and the role of metals in PAI-1 is relevant because metals have been demonstrated to play a role in the severity of inflammatory response, tumor p ...
Identification of Myosin XI Receptors in Arabidopsis Defines a
... panel) and Supplemental Movie 3 online, this reporter tagged motile bodies whose appearance was similar to those tagged by MyoB1-YFP and Myob2-YFP. Interestingly, these bodies were much more sparse compared with the denser flow of the MyoB1YFP– or MyoB2-YFP–tagged bodies. This outcome suggested that ...
... panel) and Supplemental Movie 3 online, this reporter tagged motile bodies whose appearance was similar to those tagged by MyoB1-YFP and Myob2-YFP. Interestingly, these bodies were much more sparse compared with the denser flow of the MyoB1YFP– or MyoB2-YFP–tagged bodies. This outcome suggested that ...
1 Chapter 1 Introduction 1.1 – Introduction Proteins are
... labeled antimicrobial peptides (AMPs), ranging from 15-50 amino acids function as part of the innate immunity in multicellular organisms (2). The features of proteins and peptides that contribute to their structure, stability and activity have been elucidated through an enormous body of experimental ...
... labeled antimicrobial peptides (AMPs), ranging from 15-50 amino acids function as part of the innate immunity in multicellular organisms (2). The features of proteins and peptides that contribute to their structure, stability and activity have been elucidated through an enormous body of experimental ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... the phosphorylation of myo-inositol 1,4,6-trisphosphate, myo-inositol 1,4,5,6-tetrakisphosphate, and myo-inositol 3,4,5,6tetrakisphosphate. Nuclear magnetic resonance spectroscopy analysis indicates that the phosphorylation product of myoinositol 1,4,6-trisphosphate is inositol 1,2,4,6-tetrakisphosp ...
... the phosphorylation of myo-inositol 1,4,6-trisphosphate, myo-inositol 1,4,5,6-tetrakisphosphate, and myo-inositol 3,4,5,6tetrakisphosphate. Nuclear magnetic resonance spectroscopy analysis indicates that the phosphorylation product of myoinositol 1,4,6-trisphosphate is inositol 1,2,4,6-tetrakisphosp ...
Characterization of CIC transporter proteins Moradi, Hossein
... Structural organisation of ClC transporters The Escherichia coli EcClC and Salmonella typhimurium StClC proteins were the first ClC proteins to be crystallized and provided the second structure of a transmembrane channel protein (Dutzler et al., 2002; Dutzler et al., 2003). These studies revealed th ...
... Structural organisation of ClC transporters The Escherichia coli EcClC and Salmonella typhimurium StClC proteins were the first ClC proteins to be crystallized and provided the second structure of a transmembrane channel protein (Dutzler et al., 2002; Dutzler et al., 2003). These studies revealed th ...
Substrate specificity of protein kinase A in reaction with
... were provided by Hanks, Quinn and Hunter (Hanks et al, 1988). The common motif for all protein kinases is the homologous “kinase domain”, also known as the “catalytic domain”. This part of these enzymes consists of 250-300 amino acid residues. The kinase domain contains 12 conserved subdomains and i ...
... were provided by Hanks, Quinn and Hunter (Hanks et al, 1988). The common motif for all protein kinases is the homologous “kinase domain”, also known as the “catalytic domain”. This part of these enzymes consists of 250-300 amino acid residues. The kinase domain contains 12 conserved subdomains and i ...
Co-translational Folding
... Bioinformatics (Protein Structure Prediction) – prediction of native structure from amino-acid sequences alone ...
... Bioinformatics (Protein Structure Prediction) – prediction of native structure from amino-acid sequences alone ...
The 1997 GSA Honors and Awards
... Oliver also continued to pursue a long-standing interest in the biosynthesis of starch. His earlier discovery that the waxy locus encoded a starch-bound ADP-glucose glucosyl transferase was one of the first to relate a phenotypically identified plant gene with the underlying enzymatic defect. Althou ...
... Oliver also continued to pursue a long-standing interest in the biosynthesis of starch. His earlier discovery that the waxy locus encoded a starch-bound ADP-glucose glucosyl transferase was one of the first to relate a phenotypically identified plant gene with the underlying enzymatic defect. Althou ...
EF-Tu (elongation factor thermo unstable)
... The active site of the protein is the site of the hydrolysis of GTP to GDP, which occurs to release the aatRNA molecule. The protein and its interacting amino acids along with the Mg2+ metal stabilize this active site. Without stability in the active site the hydrolysis of GTP to GDP would not be ab ...
... The active site of the protein is the site of the hydrolysis of GTP to GDP, which occurs to release the aatRNA molecule. The protein and its interacting amino acids along with the Mg2+ metal stabilize this active site. Without stability in the active site the hydrolysis of GTP to GDP would not be ab ...
Determination of Nutrient Contents and Amino acid Composition of
... CuSO4-treatment not only speed up the process of wound closure but also the quality of different tissue regeneration clearly. CuSO4- treatment hyperproliveratif associated with epithelial tissue, and saturation in the cell membrane of granulation of copper-treatment on wound into a higher expression ...
... CuSO4-treatment not only speed up the process of wound closure but also the quality of different tissue regeneration clearly. CuSO4- treatment hyperproliveratif associated with epithelial tissue, and saturation in the cell membrane of granulation of copper-treatment on wound into a higher expression ...
Rooting the Ribosomal Tree of Life Research article
... Forterre 1999; Cavalier-Smith 2006). For these reasons, a ribosomal ToL has been proposed to be an ideal ‘‘backbone’’ upon which to map horizontal gene transfers, clearly depicting their distinct contribution to genomic evolution (Gogarten 1995; Dagan et al. 2008; Swithers et al. 2009). Unfortunatel ...
... Forterre 1999; Cavalier-Smith 2006). For these reasons, a ribosomal ToL has been proposed to be an ideal ‘‘backbone’’ upon which to map horizontal gene transfers, clearly depicting their distinct contribution to genomic evolution (Gogarten 1995; Dagan et al. 2008; Swithers et al. 2009). Unfortunatel ...
-portal.org Journal of Molecular Biology
... the structural grounds for such effect. As in our previous study of membrane insertion of the PV 2B, 11 we used a well-characterized in vitro experimental system based on glycosylation 12 that accurately reports the integration of TM regions into microsomal membranes. Upon insertion, the oligosaccha ...
... the structural grounds for such effect. As in our previous study of membrane insertion of the PV 2B, 11 we used a well-characterized in vitro experimental system based on glycosylation 12 that accurately reports the integration of TM regions into microsomal membranes. Upon insertion, the oligosaccha ...