Identification of a novel gene family involved in osmotic
... sensitivity to a cuticle-disrupting treatment (Table 2). This indicates that not all Osr genes are required for the structural integrity of the C. elegans cuticle. Relationship between osmotic resistance and defecation behavior: Since osm7(sa89) was known to alter the defecation cycle period, we ex ...
... sensitivity to a cuticle-disrupting treatment (Table 2). This indicates that not all Osr genes are required for the structural integrity of the C. elegans cuticle. Relationship between osmotic resistance and defecation behavior: Since osm7(sa89) was known to alter the defecation cycle period, we ex ...
The Role of Amino Acids
... chemical properties of the amino acids of proteins determine the biological activity of the protein. Proteins not only catalyze all (or most) of the reactions in living cells, they control virtually all cellular process. In addition, proteins contain within their amino acid sequences the necessary i ...
... chemical properties of the amino acids of proteins determine the biological activity of the protein. Proteins not only catalyze all (or most) of the reactions in living cells, they control virtually all cellular process. In addition, proteins contain within their amino acid sequences the necessary i ...
Circadian Regulator CLOCK Is a Histone Acetyltransferase
... The activation of clock-controlled genes by mCLOCK: mBMAL1 was recently shown to be coupled to circadian changes in histone acetylation at their promoters (Curtis et al., 2004; Etchegaray et al., 2003; Ripperger and Schibler, 2006), indicating that transcription-permissive chromatin states are dynam ...
... The activation of clock-controlled genes by mCLOCK: mBMAL1 was recently shown to be coupled to circadian changes in histone acetylation at their promoters (Curtis et al., 2004; Etchegaray et al., 2003; Ripperger and Schibler, 2006), indicating that transcription-permissive chromatin states are dynam ...
The CamSol Method of Rational Design of Protein Mutants with
... seven gammabody mutants with a predicted increased solubility with respect to the wild-type form (Table 1 and Table S1). No particular rule was applied in selecting these seven gammabody variants. We decided to include in the set the wild type (WT in Table 1), the variant predicted to be the most so ...
... seven gammabody mutants with a predicted increased solubility with respect to the wild-type form (Table 1 and Table S1). No particular rule was applied in selecting these seven gammabody variants. We decided to include in the set the wild type (WT in Table 1), the variant predicted to be the most so ...
Regulation of microtubule dynamics by microtubule
... al., 1990), but they show extensive regional amino acid similarities including a positively-charged segment which is close to the amino terminus and contains multiple repeats of a (Lys/Arg) (Lys/Arg) (Glu/Asp) motif (Noble el al" 1989; Langkopf el al" 1992). These repeats appear to be involved in mi ...
... al., 1990), but they show extensive regional amino acid similarities including a positively-charged segment which is close to the amino terminus and contains multiple repeats of a (Lys/Arg) (Lys/Arg) (Glu/Asp) motif (Noble el al" 1989; Langkopf el al" 1992). These repeats appear to be involved in mi ...
Linking folding and binding
... sequences [1,21]. Indeed, it is frequently possible to identify these folding motifs, which have been termed molecular recognition elements or MoRFs, by bioinformatic analysis of the protein sequence [22]. These recognition motifs can fold into helix, b-strand, or form irregular structure on bindin ...
... sequences [1,21]. Indeed, it is frequently possible to identify these folding motifs, which have been termed molecular recognition elements or MoRFs, by bioinformatic analysis of the protein sequence [22]. These recognition motifs can fold into helix, b-strand, or form irregular structure on bindin ...
Endosomal transport of septin mRNA and protein indicates local
... reflecting delivery of entire translation systems (including ribosomes) to the ultimate site of synthesis. Unfortunately in this regard the key data are explained in a somewhat confusing manner. The key experiment hinges on mutants of Rrm4 that are not adequately explained for a general (or perhaps ...
... reflecting delivery of entire translation systems (including ribosomes) to the ultimate site of synthesis. Unfortunately in this regard the key data are explained in a somewhat confusing manner. The key experiment hinges on mutants of Rrm4 that are not adequately explained for a general (or perhaps ...
MEDICAL BIOCHEMISTRY Problem Unit One 1999/2000 pH and
... Water makes up about 70% of a typical cell by weight. It is one of two solvents in which most of biochemistry occurs, the second being the lipids of membranes. Water is a very unusual substance and plays a central role in defining life as we know it. Its large dipole moment means that it is a highly ...
... Water makes up about 70% of a typical cell by weight. It is one of two solvents in which most of biochemistry occurs, the second being the lipids of membranes. Water is a very unusual substance and plays a central role in defining life as we know it. Its large dipole moment means that it is a highly ...
SNARE complexes of different composition jointly
... FIGURE 1: Interaction analysis of known Q-SNARE proteins involved in cytokinesis. GFPthe mitosis-specific KNOLLE promoter KNOLLE (A), YFP-NPSN11 (B), and Myc-SNAP33 (C) were immunoprecipitated from total only partially rescued the double-mutant protein extracts of Arabidopsis inflorescences. (A) GF ...
... FIGURE 1: Interaction analysis of known Q-SNARE proteins involved in cytokinesis. GFPthe mitosis-specific KNOLLE promoter KNOLLE (A), YFP-NPSN11 (B), and Myc-SNAP33 (C) were immunoprecipitated from total only partially rescued the double-mutant protein extracts of Arabidopsis inflorescences. (A) GF ...
Protein Structure and Interactions
... In mammals small heat-shock proteins (sHsp) comprises 10 members, many of which are considered as molecular chaperones based on demonstrated in vitro activity. Whether these proteins behave as chaperones in vivo remains unclear. Here, we investigated the effect of HspB8 on the expression of Htt43Q, ...
... In mammals small heat-shock proteins (sHsp) comprises 10 members, many of which are considered as molecular chaperones based on demonstrated in vitro activity. Whether these proteins behave as chaperones in vivo remains unclear. Here, we investigated the effect of HspB8 on the expression of Htt43Q, ...
Placental Transporters Relevant to Drug Distribution across the
... that carries out a multitude of functions obligatory for normal fetal development. These functions include transfer of nutrients from the mother to the fetus, removal of metabolic waste products from the fetus, and secretion of a variety of steroid and peptide hormones into the maternal and/or fetal ...
... that carries out a multitude of functions obligatory for normal fetal development. These functions include transfer of nutrients from the mother to the fetus, removal of metabolic waste products from the fetus, and secretion of a variety of steroid and peptide hormones into the maternal and/or fetal ...
Brewer`s yeast - IBD Learning Zone
... developed to assess yeast stress resistance and evaluate the suitability of a specific strain for use in industrial ethanol fermentations. This ‘model’ could potentially be used for screening candidate yeast strains for relative stress resistance in the fuel ethanol industry and other industries whe ...
... developed to assess yeast stress resistance and evaluate the suitability of a specific strain for use in industrial ethanol fermentations. This ‘model’ could potentially be used for screening candidate yeast strains for relative stress resistance in the fuel ethanol industry and other industries whe ...
YEAST INFORMATION SHEET
... • Production of carbon dioxide. This is generated by the yeast as a result of the breakdown of fermentable sugars in the dough. The production of carbon dioxide causes expansion of the dough as it is trapped within the protein matrix of the dough. • Dough maturation. This is accomplished by the ch ...
... • Production of carbon dioxide. This is generated by the yeast as a result of the breakdown of fermentable sugars in the dough. The production of carbon dioxide causes expansion of the dough as it is trapped within the protein matrix of the dough. • Dough maturation. This is accomplished by the ch ...
1dl5 Lichtarge lab 2006
... Another column worth noting is denoted “noc/bb”; it tells the number of contacts heavy atoms of the residue in question make across the interface, as well as how many of them are realized through the backbone atoms (if all or most contacts are through the backbone, mutation presumably won’t have str ...
... Another column worth noting is denoted “noc/bb”; it tells the number of contacts heavy atoms of the residue in question make across the interface, as well as how many of them are realized through the backbone atoms (if all or most contacts are through the backbone, mutation presumably won’t have str ...
Dual transcriptional activities of SIX proteins define
... functionally interact during the induction of ectopic eye development (Seimiya and Gehring, 2000; Salzer and Kumar, 2010). These results imply that Optix may influence gene expression differently than other SIX proteins by functioning as a dedicated transcriptional repressor. In this report, we atte ...
... functionally interact during the induction of ectopic eye development (Seimiya and Gehring, 2000; Salzer and Kumar, 2010). These results imply that Optix may influence gene expression differently than other SIX proteins by functioning as a dedicated transcriptional repressor. In this report, we atte ...
Protein Cross-linkers handbook and selection guide
... with primary amines with relative efficiency at physiological pH. NHS-Esters react with α-amine groups present on the N-termini of proteins and α-amines on lysine residues to form an amide bond and release N-hydroxysuccinimide. Hydrolysis of NHS-Ester competes with the primary amine reaction. Hydrol ...
... with primary amines with relative efficiency at physiological pH. NHS-Esters react with α-amine groups present on the N-termini of proteins and α-amines on lysine residues to form an amide bond and release N-hydroxysuccinimide. Hydrolysis of NHS-Ester competes with the primary amine reaction. Hydrol ...
Regulation Of Glut4 Biogenesis In Muscle Evidence For
... g/ml fungizone, and 10% fetal bovine serum (FBS). Cells were maintained in continuous passage by trypsinization of subconfluent cultures with 0.25% trypsin. Myoblast differentiation was induced by switching to medium containing 2% heat-inactivated horse serum when myoblasts were ⬃80% confluent. Exp ...
... g/ml fungizone, and 10% fetal bovine serum (FBS). Cells were maintained in continuous passage by trypsinization of subconfluent cultures with 0.25% trypsin. Myoblast differentiation was induced by switching to medium containing 2% heat-inactivated horse serum when myoblasts were ⬃80% confluent. Exp ...
... T or F: If the ligand concentration is less than KD then the fractional saturation is greater than 0.5. [less than] T or F: Hydrogen bonds are seldom observed in protein-ligand interactions [they can be common] T or F:Ligands that differ in their KD values are more likely to have different kinetic o ...
Proteins RCA Gene Cluster and Functional RCA Locus in Chicken
... A 150-kb DNA fragment, which contains the gene of the chicken complement regulatory protein CREM (formerly named Cremp), was isolated from a microchromosome by screening bacterial artificial chromosome library. Within 100 kb of the cloned region, three complete genes encoding short consensus repeats ...
... A 150-kb DNA fragment, which contains the gene of the chicken complement regulatory protein CREM (formerly named Cremp), was isolated from a microchromosome by screening bacterial artificial chromosome library. Within 100 kb of the cloned region, three complete genes encoding short consensus repeats ...
Curriculum vitae
... 8. Hamari Z*, Amillis S*, Drevet C, Apostolaki A, Vágvölgyi C, Diallinas G and Scazzocchio C. (2009). Convergent evolution and orphan genes in the FUR4p-like family and characterisation of a general nucleoside transporter in Aspergillus nidulans. Mol Microbiol 73: 43-57. (*Equal contribution) 9. Gou ...
... 8. Hamari Z*, Amillis S*, Drevet C, Apostolaki A, Vágvölgyi C, Diallinas G and Scazzocchio C. (2009). Convergent evolution and orphan genes in the FUR4p-like family and characterisation of a general nucleoside transporter in Aspergillus nidulans. Mol Microbiol 73: 43-57. (*Equal contribution) 9. Gou ...
Figure 1000G Allele Frequencies
... S1. [11] had another rule for transcripts containing more than two 3’UTRs in the transcript. However, we observed that the stop codons in Ensembl gene annotation did not follow this particular rule, so we eliminated this rule and simply followed rule 2 if there was more than one 3’ UTR. c. Eukaryoti ...
... S1. [11] had another rule for transcripts containing more than two 3’UTRs in the transcript. However, we observed that the stop codons in Ensembl gene annotation did not follow this particular rule, so we eliminated this rule and simply followed rule 2 if there was more than one 3’ UTR. c. Eukaryoti ...
Evolution of hard proteins in the sauropsid integument in relation to
... Fig. 1 Macroscopic aspect of reptilian scales (A–D) and histology of the epidermis in scales of different reptiles (E–M). (A) Overlapped trunk scale of snake (Natrix natrix). Bar, 0.5 mm. (B) Little overlapping scale of ventral region of midtrunk region of the tuatara (Sphenodon punctatus). Bar, 0.5 ...
... Fig. 1 Macroscopic aspect of reptilian scales (A–D) and histology of the epidermis in scales of different reptiles (E–M). (A) Overlapped trunk scale of snake (Natrix natrix). Bar, 0.5 mm. (B) Little overlapping scale of ventral region of midtrunk region of the tuatara (Sphenodon punctatus). Bar, 0.5 ...
Function and biotechnology of extremophilic enzymes in low water
... salt-in mechanism for osmotic stabilization (e.g. halophilic archaea and some halophilic bacteria) [8]. Such halophilic proteins have evolved a specific set of molecular features that help them to compete with ions for water and maintain a stable hydration shell. In fact, in comparison to non-haloph ...
... salt-in mechanism for osmotic stabilization (e.g. halophilic archaea and some halophilic bacteria) [8]. Such halophilic proteins have evolved a specific set of molecular features that help them to compete with ions for water and maintain a stable hydration shell. In fact, in comparison to non-haloph ...