Review Structural glycobiology: A game of snakes and ladders
... To help explore the concepts of the carbohydrate structure and recognition, let us compare carbohydrates to another flexible object, a snake. To the extent that a living snake is a flexible 3D object that is not random in its motional properties, it serves as a useful analogy for carbohydrate struct ...
... To help explore the concepts of the carbohydrate structure and recognition, let us compare carbohydrates to another flexible object, a snake. To the extent that a living snake is a flexible 3D object that is not random in its motional properties, it serves as a useful analogy for carbohydrate struct ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... motility [23]. Additionally, when Hsp90Ec is overexpressed in E. coli, cells filament and become sensitive to SDS [5]. Both eukaryotic Hsp90 and Hsp90Ec have been shown to remodel proteins in vitro. For example, eukaryotic Hsp90 reactivates denatured luciferase in conjunction with Hop/Sti1, Hsp70 an ...
... motility [23]. Additionally, when Hsp90Ec is overexpressed in E. coli, cells filament and become sensitive to SDS [5]. Both eukaryotic Hsp90 and Hsp90Ec have been shown to remodel proteins in vitro. For example, eukaryotic Hsp90 reactivates denatured luciferase in conjunction with Hop/Sti1, Hsp70 an ...
Structural Basis of Biological Nitrogen Fixation
... the purine ring was bound to one subunit and the phosphates to the other (Figure 2). The ADP in this binding mode interacts with the two well-known amino acid sequences characteristic of nucleotidebinding proteins;49,50 GXXXXGKS (the Walker motif A) which is present between residues 9 to 16, and the ...
... the purine ring was bound to one subunit and the phosphates to the other (Figure 2). The ADP in this binding mode interacts with the two well-known amino acid sequences characteristic of nucleotidebinding proteins;49,50 GXXXXGKS (the Walker motif A) which is present between residues 9 to 16, and the ...
Cloning and sequencing of a gene encoding acidophilic amylase
... fragment was cloned from a AgtlO gene bank of B. acidocaldarius chromosomal DNA by plaque hybridization. The oligonucleotide 371 (Table 1) was synthesized according to the deduced DNA sequence of one of the lysylendopeptidase-cleavedpeptides (Table 1, Fig. 2). It hybridized with six plaques out of 7 ...
... fragment was cloned from a AgtlO gene bank of B. acidocaldarius chromosomal DNA by plaque hybridization. The oligonucleotide 371 (Table 1) was synthesized according to the deduced DNA sequence of one of the lysylendopeptidase-cleavedpeptides (Table 1, Fig. 2). It hybridized with six plaques out of 7 ...
Receptor-mediated sorting of soluble vacuolar proteins: myths, facts
... the organellar proteome of Arabidopsis has identified four different VSRs at three separate locations by principal components analysis. One of them is very close to a V0a subunit of a V-ATPase subunit, pointing to a TGN location (Dunkley et al., 2006). This suggests a functional specialization of di ...
... the organellar proteome of Arabidopsis has identified four different VSRs at three separate locations by principal components analysis. One of them is very close to a V0a subunit of a V-ATPase subunit, pointing to a TGN location (Dunkley et al., 2006). This suggests a functional specialization of di ...
The maize ID1 flowering time regulator is a zinc finger protein with
... Site-directed mutagenesis was introduced by three-step PCR, using the same protocol as described above. Full-length id1 cDNA was used as template in the ®rst and second PCR ampli®cations. In the ®rst PCR, the forward primer IDEcoRIF and reverse primers containing the desired mutation were used. In t ...
... Site-directed mutagenesis was introduced by three-step PCR, using the same protocol as described above. Full-length id1 cDNA was used as template in the ®rst and second PCR ampli®cations. In the ®rst PCR, the forward primer IDEcoRIF and reverse primers containing the desired mutation were used. In t ...
The Amino Acid Sequence Contains Leucine-Rich
... many dialysis steps using various buffers before each chromatography. W e used only one buffer system through all chromatographies, and all the procedures can be easily done within 4 days. As to the yield, we can easily purify 200 pg of GPV from 10l2platelets. This high yield also seems to have an a ...
... many dialysis steps using various buffers before each chromatography. W e used only one buffer system through all chromatographies, and all the procedures can be easily done within 4 days. As to the yield, we can easily purify 200 pg of GPV from 10l2platelets. This high yield also seems to have an a ...
Poster
... effectively diagnosed while in incubation. Brain sections of postmortem CJD patients (lower) exhibit sponge-like holes compared to healthy brain sections (upper). There are currently no cures for prion diseases and prions are resistant to common methods of sterilization. ...
... effectively diagnosed while in incubation. Brain sections of postmortem CJD patients (lower) exhibit sponge-like holes compared to healthy brain sections (upper). There are currently no cures for prion diseases and prions are resistant to common methods of sterilization. ...
Transport Processes of Solutes across the Vacuolar Membrane of
... amino acid transport across the tonoplast of higher plants is limited. The most prominent amino acid transport system, observed first in barley (Dietz et al. 1990, Martinoia et al. 1991, Görlach and Willms-Hoff 1992) and subsequently in other plants (Thume and Dietz 1991), is a carrier or channel wh ...
... amino acid transport across the tonoplast of higher plants is limited. The most prominent amino acid transport system, observed first in barley (Dietz et al. 1990, Martinoia et al. 1991, Görlach and Willms-Hoff 1992) and subsequently in other plants (Thume and Dietz 1991), is a carrier or channel wh ...
magamtol talalt cikkek
... Complementary DNA encoding a catalytic subunit of protein phosphatase 1, PP1 87B, hybridises at four positions (87B, 9C, 13C and 96A) to Drosophila melanogaster polytene chromosomes, three of which are known to be expressed [Dombrádi, V., Axton, J.M., Brewis, N.D., Da Cruz e Silva, E.F., Alphey, L. ...
... Complementary DNA encoding a catalytic subunit of protein phosphatase 1, PP1 87B, hybridises at four positions (87B, 9C, 13C and 96A) to Drosophila melanogaster polytene chromosomes, three of which are known to be expressed [Dombrádi, V., Axton, J.M., Brewis, N.D., Da Cruz e Silva, E.F., Alphey, L. ...
Full Text
... finding these and other correlations, and an inference program for searching databases with Bayesian networks. Thus, we have made a first step in bringing critical structural information in the form of correlations into the realm of sequence analysis. In this paper we demonstrate the discovery and r ...
... finding these and other correlations, and an inference program for searching databases with Bayesian networks. Thus, we have made a first step in bringing critical structural information in the form of correlations into the realm of sequence analysis. In this paper we demonstrate the discovery and r ...
Cytoskeletal Drugs and Gravity-Induced Lateral Auxin Transport in
... coleoptiles of about 15 mm in length were placed, with the gravity vector pointing from tip towards base, in Eppendorf tubes containing water (control) or aqueous solutions of various cytoskeletal drugs, and kept for 30 min in the same orientation. They were then inverted for various time intervals, ...
... coleoptiles of about 15 mm in length were placed, with the gravity vector pointing from tip towards base, in Eppendorf tubes containing water (control) or aqueous solutions of various cytoskeletal drugs, and kept for 30 min in the same orientation. They were then inverted for various time intervals, ...
View/Open - VUW research archive - Victoria University of Wellington
... Bede Busby, Peter Birchirm, Katie Zeier, and Dr Christine Stockholm. Thank you for sharing your time and friendship. Lastly, to my family, you have been such a great support group. Thank you all so much!!! ...
... Bede Busby, Peter Birchirm, Katie Zeier, and Dr Christine Stockholm. Thank you for sharing your time and friendship. Lastly, to my family, you have been such a great support group. Thank you all so much!!! ...
Free amino acids and proteins dynamics in somatic embryogenesis
... (Ortiz-Lopez et al., 2000). In MS/2 medium, free amino acids contents was low in embryogenic calli and then increased significantly during globular and bipolar embryos formation. These results are similar to those obtained in Pinus patula (Malabadi and van Staden, 2005) and in Theobroma cacao (Nieme ...
... (Ortiz-Lopez et al., 2000). In MS/2 medium, free amino acids contents was low in embryogenic calli and then increased significantly during globular and bipolar embryos formation. These results are similar to those obtained in Pinus patula (Malabadi and van Staden, 2005) and in Theobroma cacao (Nieme ...
Allosteric regulation and catalysis emerge via a common
... conformational change is not ligand induced. Rather, the role of ligand binding is to stabilize a pre-existing conformation, selecting this conformation in the population of protein conformers. This study highlights the unique suitability of NMR spectroscopy for studying protein dynamics: NMR relaxa ...
... conformational change is not ligand induced. Rather, the role of ligand binding is to stabilize a pre-existing conformation, selecting this conformation in the population of protein conformers. This study highlights the unique suitability of NMR spectroscopy for studying protein dynamics: NMR relaxa ...
Anatomy of the red cell membrane skeleton: unanswered questions
... other proteins. The details of spectrin structure are shown in Figure 3. On average, six spectrins bind per actin filament, leading to a pseudohexagonal arrangement.26 Isolated α- and β-spectrin chains bind to each other electrostatically at a pair of nucleation sites formed by repeats near the spec ...
... other proteins. The details of spectrin structure are shown in Figure 3. On average, six spectrins bind per actin filament, leading to a pseudohexagonal arrangement.26 Isolated α- and β-spectrin chains bind to each other electrostatically at a pair of nucleation sites formed by repeats near the spec ...
Novel Inhibitory Action of Tunicamycin Homologues Suggests a
... TM also has been found to interfere with the intracellular targeting of non-glycosylated proteins, including the appearance in a membrane fraction of newly synthesized GAP-43, a major component of neuronal growth cones (PerroneBizzozero and Benowitz, 1987). Because membrane association of GAP-43 fol ...
... TM also has been found to interfere with the intracellular targeting of non-glycosylated proteins, including the appearance in a membrane fraction of newly synthesized GAP-43, a major component of neuronal growth cones (PerroneBizzozero and Benowitz, 1987). Because membrane association of GAP-43 fol ...
Experimental Analysis of the Rice Mitochondrial
... rice mitochondrial proteins was identified and listed in the rice proteome database (Komatsu, 2005) using mitochondria isolated by Suc gradient centrifugation and gel-based spot analysis. However, there is less than 20% overlap between the protein lists reported in these two studies. The removal of ...
... rice mitochondrial proteins was identified and listed in the rice proteome database (Komatsu, 2005) using mitochondria isolated by Suc gradient centrifugation and gel-based spot analysis. However, there is less than 20% overlap between the protein lists reported in these two studies. The removal of ...
From Sequence to Structure
... structure (or fold) under physiological conditions. On the other hand, the demands of protein function require that the folded protein should not be too rigid. Presumably because of these constraints, the number of folds adopted by proteins, though large, is limited. Whether the limited number of fo ...
... structure (or fold) under physiological conditions. On the other hand, the demands of protein function require that the folded protein should not be too rigid. Presumably because of these constraints, the number of folds adopted by proteins, though large, is limited. Whether the limited number of fo ...
Interactions of liposomes and lipid-based carrier systems with blood
... clearance properties in the biological milieu. ...
... clearance properties in the biological milieu. ...
Genome-wide transcription profiling of aerobic and anaerobic
... As the growth of anaerobic biofilms of laboratory E. coli strains was unsuccessful, studies focused on planktonic cultures, elucidating the transition patterns from aerobic to microaerobic and anaerobic environments (Partridge et al. 2006; Trotter et al. 2011). But, E. coli genome-wide analyses have ...
... As the growth of anaerobic biofilms of laboratory E. coli strains was unsuccessful, studies focused on planktonic cultures, elucidating the transition patterns from aerobic to microaerobic and anaerobic environments (Partridge et al. 2006; Trotter et al. 2011). But, E. coli genome-wide analyses have ...
2.3.1. Vector construction - Trace: Tennessee Research and
... turn, these interactions lead to the assembly and organization of a cell. Yet, unraveling the complexity of interactions both within the intracellular and extracellular environment is a difficult challenge for biologists. Recent advances in genomic sequencing/annotation have led to an increased rate ...
... turn, these interactions lead to the assembly and organization of a cell. Yet, unraveling the complexity of interactions both within the intracellular and extracellular environment is a difficult challenge for biologists. Recent advances in genomic sequencing/annotation have led to an increased rate ...
