Recombinant expression and characterisation of monofunctional S-
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
A Study of Phylogenetic Relationships and Homology of
... overall species phenotype. For example, both humans and chimpanzees have many similar anatomical characters and functions, so we might expect their proteins to be similar, regardless of whether they are genealogically related or not. However, we can compare the sequences of basic genes that are used ...
... overall species phenotype. For example, both humans and chimpanzees have many similar anatomical characters and functions, so we might expect their proteins to be similar, regardless of whether they are genealogically related or not. However, we can compare the sequences of basic genes that are used ...
- Wiley Online Library
... drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. The full contents can be found at http://onlinelibrary.wiley.com/doi/10. 1111/bph.13347/full. This compilation of the major pharmacological targets is divided into eight ...
... drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. The full contents can be found at http://onlinelibrary.wiley.com/doi/10. 1111/bph.13347/full. This compilation of the major pharmacological targets is divided into eight ...
Hydrophobic-at-Interface Regions in Viral Fusion Protein Ectodomains
... couple dansyl-Trp is approximately 17 Å (Vaz and Schoellmann, 1976), the observed strong quenching of Trp fluorescence suggests that these residues remain associated to the interface and兾or to the acyl chain region close to it. The pre-TM region is immediately followed by the membrane spanning sequ ...
... couple dansyl-Trp is approximately 17 Å (Vaz and Schoellmann, 1976), the observed strong quenching of Trp fluorescence suggests that these residues remain associated to the interface and兾or to the acyl chain region close to it. The pre-TM region is immediately followed by the membrane spanning sequ ...
From Sequence to Function
... in several model organisms. These databases can also be searched for insights into the functions of these proteins and their corresponding proteins in other organisms. Sequence and structural comparison can usually give only limited information, however, and comprehensively characterizing the functi ...
... in several model organisms. These databases can also be searched for insights into the functions of these proteins and their corresponding proteins in other organisms. Sequence and structural comparison can usually give only limited information, however, and comprehensively characterizing the functi ...
NIH Public Access - University of Utah Health Care
... As part of an ongoing effort to characterize mitochondrial proteins that are conserved through evolution, we initiated studies of the MPC protein family (originally designated BRP44 and BRP44L in humans) (8). This family contains three members in Saccharomyces cerevisiae, encoded by YGL080W, YHR162W ...
... As part of an ongoing effort to characterize mitochondrial proteins that are conserved through evolution, we initiated studies of the MPC protein family (originally designated BRP44 and BRP44L in humans) (8). This family contains three members in Saccharomyces cerevisiae, encoded by YGL080W, YHR162W ...
PDZ proteins retain and regulate membrane transporters in
... with the apical brush-border membrane in polarized epithelial cells. NHERF-1 was originally cloned as an essential cofactor for the protein kinase A (PKA)-mediated inhibition of NHE3 in renal proximal tubule cells (66, 77). The human ortholog of NHERF-1 is also known as EBP50 (ezrin-radixin-moesinbi ...
... with the apical brush-border membrane in polarized epithelial cells. NHERF-1 was originally cloned as an essential cofactor for the protein kinase A (PKA)-mediated inhibition of NHE3 in renal proximal tubule cells (66, 77). The human ortholog of NHERF-1 is also known as EBP50 (ezrin-radixin-moesinbi ...
the pdf - University of British Columbia
... investigated by Okado and Oppenheim (1985). Using the retrograde tract-tracers wheat germ agglutinated-horseraddish peroxidase (WGA-HRP), they injected lumbar spinal cords of chicks from all 21 embryonic days of age. Subsequent sectioning and analysis of brainstem nuclei, for labelled neuronal cell ...
... investigated by Okado and Oppenheim (1985). Using the retrograde tract-tracers wheat germ agglutinated-horseraddish peroxidase (WGA-HRP), they injected lumbar spinal cords of chicks from all 21 embryonic days of age. Subsequent sectioning and analysis of brainstem nuclei, for labelled neuronal cell ...
Mechanisms Shaping the Membranes of Cellular Organelles
... one, whereas during fission a continuous membrane is divided into two separate ones. For example, ER tubules can fuse with an existing tubule to form a three-way junction. How the characteristic shape of an organelle is achieved and how membrane curvature is generated are still only poorly understood ...
... one, whereas during fission a continuous membrane is divided into two separate ones. For example, ER tubules can fuse with an existing tubule to form a three-way junction. How the characteristic shape of an organelle is achieved and how membrane curvature is generated are still only poorly understood ...
Article, Discoveries Variation in mutational robustness between
... base pair substitutions and all of them were classified as “neutral”, which here means they cannot be reliably distinguished from wild-type controls (jsj < 0.004, in the shaded area in fig. 2A–C, full details in supplementary table S1, Supplementary Material online). The mean cost for all synonymous ...
... base pair substitutions and all of them were classified as “neutral”, which here means they cannot be reliably distinguished from wild-type controls (jsj < 0.004, in the shaded area in fig. 2A–C, full details in supplementary table S1, Supplementary Material online). The mean cost for all synonymous ...
Contribution of molecular chaperones to protein folding in the
... How do proteins fold in the cell? The process by which a linear polypeptide attains its unique, functionally active, three-dimensional structure from the astronomical number of possible conformations has perplexed researchers for many years [1]. Most of our knowledge concerning the folding and assem ...
... How do proteins fold in the cell? The process by which a linear polypeptide attains its unique, functionally active, three-dimensional structure from the astronomical number of possible conformations has perplexed researchers for many years [1]. Most of our knowledge concerning the folding and assem ...
The Proteomic Code: a molecular recognition code for proteins
... found that these specially coded amino acid pairs are statistically overrepresented in those proteins known to interact with each other. In addition, I was able to find short complementary amino acid sequences within the same protein sequences and inferred that these might play a role in the formati ...
... found that these specially coded amino acid pairs are statistically overrepresented in those proteins known to interact with each other. In addition, I was able to find short complementary amino acid sequences within the same protein sequences and inferred that these might play a role in the formati ...
Phospholipid synthesis in Borrelia burgdorferi: BB0249 and BB0721
... protocols (Ames, 1968) and TLC of the extracts was performed. Staining of the TLC plates confirmed that PC and PG are the major phospholipids in the B. burgdorferi membrane (data not shown) and suggested that this method was a reliable method for examining B. burgdorferi membrane phospholipids. We t ...
... protocols (Ames, 1968) and TLC of the extracts was performed. Staining of the TLC plates confirmed that PC and PG are the major phospholipids in the B. burgdorferi membrane (data not shown) and suggested that this method was a reliable method for examining B. burgdorferi membrane phospholipids. We t ...
Leukaemia Section Anaplasic large cell lymphoma (ALCL) Atlas of Genetics and Cytogenetics
... The common type, characterized by large lymphoid cells with horseshoe shaped nuclei with many nucleoli, and large cytoplasm; may be ALK + or - ALCL; The small cell type, together with the above described cells, show small and medium sized cells; almost exclusively ALK+ cases; The lymphohistiocytic t ...
... The common type, characterized by large lymphoid cells with horseshoe shaped nuclei with many nucleoli, and large cytoplasm; may be ALK + or - ALCL; The small cell type, together with the above described cells, show small and medium sized cells; almost exclusively ALK+ cases; The lymphohistiocytic t ...
Scientific Validation
... Other yeast producers use mutagens (agents causing mutations) on commercial yeast strains, to create low-H2S strains. These mutagens have a “scattershot” effect, causing a number of different mutations. The resultant mutants are screened for low- or no-H2S producing isolates, but other mutations are ...
... Other yeast producers use mutagens (agents causing mutations) on commercial yeast strains, to create low-H2S strains. These mutagens have a “scattershot” effect, causing a number of different mutations. The resultant mutants are screened for low- or no-H2S producing isolates, but other mutations are ...
A Guide to the Analysis and Purification of Proteins and
... protein therapeutic products and to analyze these for product identity and impurities. Reversed-phase HPLC plays a vital role in the separation of peptides from digested proteomes prior to protein identification by mass spectrometry. It is also used to purify many proteins and peptides during invest ...
... protein therapeutic products and to analyze these for product identity and impurities. Reversed-phase HPLC plays a vital role in the separation of peptides from digested proteomes prior to protein identification by mass spectrometry. It is also used to purify many proteins and peptides during invest ...
Crossing borders to bind proteins—a new concept in protein
... According to this concept a fully assembled binder molecule consists of a small organic molecule or peptide that is linked with or without an interconnecting spacer to a 42-residue polypeptide selected from a set of only sixteen sequences (Fig. 1) [12]. The small molecule must bind to the protein bu ...
... According to this concept a fully assembled binder molecule consists of a small organic molecule or peptide that is linked with or without an interconnecting spacer to a 42-residue polypeptide selected from a set of only sixteen sequences (Fig. 1) [12]. The small molecule must bind to the protein bu ...
Mitofilin complexes: conserved organizers of mitochondrial
... detailed structure-function analysis of the yeast mitofilin protein led to the isolation of a truncated variant that interacts efficiently with both the SAM and TOM complexes in the outer membrane, whereas crista junctions are virtually lost (Zerbes et al., 2012). This mutant form of mitofilin lacks ...
... detailed structure-function analysis of the yeast mitofilin protein led to the isolation of a truncated variant that interacts efficiently with both the SAM and TOM complexes in the outer membrane, whereas crista junctions are virtually lost (Zerbes et al., 2012). This mutant form of mitofilin lacks ...
Journal of Bacteriology
... Discovery of three large trans-AT PKS gene clusters in B. amyloliquefaciens. Three PKS gene clusters together covering 196,340 bp (Fig. 1) were found at sites around 1.4 Mbp (pks2), 1.7 Mbp (pks1), and 2.3 Mbp (pks3) distant clockwise from the replication origin of the B. amyloliquefaciens genome (2 ...
... Discovery of three large trans-AT PKS gene clusters in B. amyloliquefaciens. Three PKS gene clusters together covering 196,340 bp (Fig. 1) were found at sites around 1.4 Mbp (pks2), 1.7 Mbp (pks1), and 2.3 Mbp (pks3) distant clockwise from the replication origin of the B. amyloliquefaciens genome (2 ...
NUCLEAR PROTEINS II. Similarity of Nonhistone Proteins in
... a- and /3-Tropomyosin (16, 17) consistently comigrated with the bands H10 and H5, respectively. Preliminary studies (see below) suggest that these are H n R N P proteins rather than a- and fltropomyosin. Bands C10, D1, and D4, which occur in both the washes and are prominent on washed nuclei, have b ...
... a- and /3-Tropomyosin (16, 17) consistently comigrated with the bands H10 and H5, respectively. Preliminary studies (see below) suggest that these are H n R N P proteins rather than a- and fltropomyosin. Bands C10, D1, and D4, which occur in both the washes and are prominent on washed nuclei, have b ...
Histone Deacetylase 4 Antibody
... members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deace ...
... members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deace ...
Host cell protein analysis by mass spectrometry and its application
... individual proteins down to single digit ppm (ng/mg) ...
... individual proteins down to single digit ppm (ng/mg) ...
Safety Assessment of Milk Proteins and Protein Derivatives as Used
... hydrolysis of casein…or lactalbumin (whey protein) (21 CFR §184.1553). Additionally, Casein is GRAS as substances migrating to food from paper and paperboard products (21CFR §182.90). Sodium Caseinate is GRAS for human and animal consumption (21CFR§182.1748, 21CFR§582.1748). Whey is GRAS for human c ...
... hydrolysis of casein…or lactalbumin (whey protein) (21 CFR §184.1553). Additionally, Casein is GRAS as substances migrating to food from paper and paperboard products (21CFR §182.90). Sodium Caseinate is GRAS for human and animal consumption (21CFR§182.1748, 21CFR§582.1748). Whey is GRAS for human c ...
