Expressing the carotenoid biosynthesis pathway in
... cerevisiae that produces β-carotene, the precursor to vitamin A When this GMO yeast is added to bread or other baked goods, it produces β-carotene in addition to its normal byproducts ...
... cerevisiae that produces β-carotene, the precursor to vitamin A When this GMO yeast is added to bread or other baked goods, it produces β-carotene in addition to its normal byproducts ...
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... Bacteria (E. coli) the SSU rRNA is 1542 nucleotides long and the LSU’s major rRNA is nearly 2906 nucleotides long, with an additional 5S RNA in the LSU. Carl Woese and co-workers [16] exploited the deep evolutionary information found within the ribosomal RNA. From which, they were able for the first ...
... Bacteria (E. coli) the SSU rRNA is 1542 nucleotides long and the LSU’s major rRNA is nearly 2906 nucleotides long, with an additional 5S RNA in the LSU. Carl Woese and co-workers [16] exploited the deep evolutionary information found within the ribosomal RNA. From which, they were able for the first ...
emboj200925-sup
... Liquid cultures of B. subtilis strains were grown in Difco Antibiotic Medium 3 (PAB), CH medium (Nicholson and Setlow, 1990), or S-medium (Karamata and Gross, 1970) at 37°C. Nutrient agar (Oxoid) plates were used for growth on solid medium, Modified Salts Medium plates with defined Mg2+ concentratio ...
... Liquid cultures of B. subtilis strains were grown in Difco Antibiotic Medium 3 (PAB), CH medium (Nicholson and Setlow, 1990), or S-medium (Karamata and Gross, 1970) at 37°C. Nutrient agar (Oxoid) plates were used for growth on solid medium, Modified Salts Medium plates with defined Mg2+ concentratio ...
Vacuolar transporters and their essential role in plant metabolism
... different internal acidic organelles in eukaryotic cells. In addition, this type of proton pump has been found on the plasma membrane of both insects and some bacterial species. The V-ATPase has an intricate structure which is ...
... different internal acidic organelles in eukaryotic cells. In addition, this type of proton pump has been found on the plasma membrane of both insects and some bacterial species. The V-ATPase has an intricate structure which is ...
Chapter 4 Effects of pH and varying concentrations of
... Figure 4.5: Effect of sodium nitrate on carbohydrate and protein of S. coactile. In the present study BG-11 medium with out sodium nitrate is the control. Morphological study of S. coactile treated with various concentration of sodium nitrate (Table 4.2) showed that false branch frequency gradually ...
... Figure 4.5: Effect of sodium nitrate on carbohydrate and protein of S. coactile. In the present study BG-11 medium with out sodium nitrate is the control. Morphological study of S. coactile treated with various concentration of sodium nitrate (Table 4.2) showed that false branch frequency gradually ...
Biochimica et Biophysica Acta
... A bacterial two-plasmid system has been previously described for studying RNA–protein interactions [21]. In this system, a first plasmid contains a modified lacZ reporter gene with an RNA-binding element located close to the translation initiation region. A second plasmid expresses the RNA-binding pro ...
... A bacterial two-plasmid system has been previously described for studying RNA–protein interactions [21]. In this system, a first plasmid contains a modified lacZ reporter gene with an RNA-binding element located close to the translation initiation region. A second plasmid expresses the RNA-binding pro ...
Ontogenetic Changes in the Rates of Protein Synthesis and
... Among fetuses, arterial oxygen saturation ranged between 56 and 75% (mean 68%), umbilical venous oxygen saturation between 86 and 94% (mean 91 %), and arterial lactate between 0.7 and 1.5 mM (mean 1.1 mM). Individual fetal and placental weights, umbilical blood flows, and oxygen uptakes were as show ...
... Among fetuses, arterial oxygen saturation ranged between 56 and 75% (mean 68%), umbilical venous oxygen saturation between 86 and 94% (mean 91 %), and arterial lactate between 0.7 and 1.5 mM (mean 1.1 mM). Individual fetal and placental weights, umbilical blood flows, and oxygen uptakes were as show ...
Cloning and functional characterization of a new subtype of the
... instead of NaCl. This maneuver suppressed the constitutively expressed basal amino acid uptake activity in these cells, which made it ideal to measure the activity of the heterologously expressed SN2. In addition to this, the uptake buffer also contained 2 mM leucine to reduce the basal amino acid u ...
... instead of NaCl. This maneuver suppressed the constitutively expressed basal amino acid uptake activity in these cells, which made it ideal to measure the activity of the heterologously expressed SN2. In addition to this, the uptake buffer also contained 2 mM leucine to reduce the basal amino acid u ...
the Golgi Apparatus as the central station of the
... The great routes of the intracellular traffic of membranes The intracellular traffic of membranes directs lipids and proteins to their destination. It has several pathways (Fig. 1): 1. The secretory, biosynthetic or exocytic pathway. This intracellular route is followed by the molecules synthesized ...
... The great routes of the intracellular traffic of membranes The intracellular traffic of membranes directs lipids and proteins to their destination. It has several pathways (Fig. 1): 1. The secretory, biosynthetic or exocytic pathway. This intracellular route is followed by the molecules synthesized ...
Analysis of expressed sequence tags from Cryptomeria japonica
... the male partner in sexual reproduction, but also because it serves as a good model for investigations of cell growth and morphogenesis. A hybridization kinetics analysis of [ 3H]cDNA with poly(A)+ RNA revealed that between 20,000 and 24,000 different mRNA sequences are present in pollen of Tradesca ...
... the male partner in sexual reproduction, but also because it serves as a good model for investigations of cell growth and morphogenesis. A hybridization kinetics analysis of [ 3H]cDNA with poly(A)+ RNA revealed that between 20,000 and 24,000 different mRNA sequences are present in pollen of Tradesca ...
Protein Synthesis
... commonly designated by their “S values,” which refer to their rate of sedimentation in an ultracentrifuge. Despite the differences in the number and size of their rRNA and protein components, both procaryotic and eucaryotic ribosomes have nearly the same structure and they function similarly. Althou ...
... commonly designated by their “S values,” which refer to their rate of sedimentation in an ultracentrifuge. Despite the differences in the number and size of their rRNA and protein components, both procaryotic and eucaryotic ribosomes have nearly the same structure and they function similarly. Althou ...
SEDS proteins are a widespread family of bacterial
... Francisella tularensis and Chlamydia trachomatis, possess a cell wall but do not encode aPBPs. Among these taxa are organisms that were recently discovered to make PG32–35 or encode an essential lipid II biosynthetic pathway36. In all cases, these organisms contain at least one SEDS family member a ...
... Francisella tularensis and Chlamydia trachomatis, possess a cell wall but do not encode aPBPs. Among these taxa are organisms that were recently discovered to make PG32–35 or encode an essential lipid II biosynthetic pathway36. In all cases, these organisms contain at least one SEDS family member a ...
Tangping Li, Ke Fan, Jun Wang and Wei Wang Reduction of protein
... the recognition tests with the reduced alphabets are discussed. By using a simpli®ed BLOSUM matrix based on these schemes, we perform an `all-against-all' sequence alignment and make coverage detection on the distantly related homologous proteins throughout the database SCOP40 (Brenner et al., 1998) ...
... the recognition tests with the reduced alphabets are discussed. By using a simpli®ed BLOSUM matrix based on these schemes, we perform an `all-against-all' sequence alignment and make coverage detection on the distantly related homologous proteins throughout the database SCOP40 (Brenner et al., 1998) ...
Elicitors, Effectors, and R Genes: The New Paradigm and a Lifetime
... The plant basal immune system can detect broadly present microbeassociated molecular patterns (MAMPs, also called PAMPs) and induce defenses, but adapted microbes express a suite of effector proteins that often act to suppress these defenses. Plants have evolved other receptors (R proteins) that det ...
... The plant basal immune system can detect broadly present microbeassociated molecular patterns (MAMPs, also called PAMPs) and induce defenses, but adapted microbes express a suite of effector proteins that often act to suppress these defenses. Plants have evolved other receptors (R proteins) that det ...
(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts
... trophic yeast Hansenula polymorpha TKL functions as a formaldehyde-accepting dihydroxyacetone synthase (DHAS) during methanol assimilation [ 19]. Comparatively little is known about the plant transketolase enzyme. Some previous studies indicated that higher plants in general might possess chloroplas ...
... trophic yeast Hansenula polymorpha TKL functions as a formaldehyde-accepting dihydroxyacetone synthase (DHAS) during methanol assimilation [ 19]. Comparatively little is known about the plant transketolase enzyme. Some previous studies indicated that higher plants in general might possess chloroplas ...
Super-resolution microscopy of mitochondria
... located in the innermost mitochondrial compartment, the aqueous matrix [55,56]. The nucleoids are distributed throughout the mitochondrial network. In humans, the mtDNA encodes 13 proteins, which are essential for the function of OXPHOS. An important and still not conclusively answered question is w ...
... located in the innermost mitochondrial compartment, the aqueous matrix [55,56]. The nucleoids are distributed throughout the mitochondrial network. In humans, the mtDNA encodes 13 proteins, which are essential for the function of OXPHOS. An important and still not conclusively answered question is w ...
Passenger Protein Determines Translocation Versus Retention in
... 100 mM sodium carbonate solution (pH, 11.4) on ice for 15 minutes. The samples were ultracentrifuged to separate soluble fraction from the membranous fraction. In experiment with methotrexate (MTX), 100 nM was used as the final concentration. All radioactive electrophoresed bands were visualized thr ...
... 100 mM sodium carbonate solution (pH, 11.4) on ice for 15 minutes. The samples were ultracentrifuged to separate soluble fraction from the membranous fraction. In experiment with methotrexate (MTX), 100 nM was used as the final concentration. All radioactive electrophoresed bands were visualized thr ...
C urrent and prospective applications of metal ion–protein
... proteins is largely governed by exposed histidine residues on the protein surface [52,58]. Cysteine also displays strong metal affinity, although to a somewhat lesser extent than histidine [33,38,59]. The metal affinity of these two amino acids can be largely attributed to their functional groups, i ...
... proteins is largely governed by exposed histidine residues on the protein surface [52,58]. Cysteine also displays strong metal affinity, although to a somewhat lesser extent than histidine [33,38,59]. The metal affinity of these two amino acids can be largely attributed to their functional groups, i ...
HSP101 functions as a specific translational
... have to be cellular in origin. Previously, we reported that a 102-kD protein (p102) was an RNA-binding protein that binds to ⍀ within the poly(CAA) subsequence responsible for translational enhancement (Leathers et al. 1993; Tanguay and Gallie 1996). RNA-binding assays and Western analysis revealed ...
... have to be cellular in origin. Previously, we reported that a 102-kD protein (p102) was an RNA-binding protein that binds to ⍀ within the poly(CAA) subsequence responsible for translational enhancement (Leathers et al. 1993; Tanguay and Gallie 1996). RNA-binding assays and Western analysis revealed ...
1. Transport of drugs across the inner and outer blood
... also shown to be poorly transportable substrates. In contrast, phenylglycinemustard was very effective in inducing the efflux of [3H]phenylalanine, suggesting that this particular amino acid mustard is an effective transportable substrate for LAT1. Even though L-DOPA and certain amino acid-mustards ...
... also shown to be poorly transportable substrates. In contrast, phenylglycinemustard was very effective in inducing the efflux of [3H]phenylalanine, suggesting that this particular amino acid mustard is an effective transportable substrate for LAT1. Even though L-DOPA and certain amino acid-mustards ...
The putative phosphatase All1758 is necessary for normal growth
... The translational all1758–gfp reporter fusion under the control of the all1758 promoter was created using primers Pall1758 BamHI F and all1758 SmaI R to amplify the 1866 bp fragment from the chromosome. The fragment was moved as a BamHI–SmaI fragment into pSMC232, a replicating plasmid used to gener ...
... The translational all1758–gfp reporter fusion under the control of the all1758 promoter was created using primers Pall1758 BamHI F and all1758 SmaI R to amplify the 1866 bp fragment from the chromosome. The fragment was moved as a BamHI–SmaI fragment into pSMC232, a replicating plasmid used to gener ...
Proteomics of Plasma Membranes from Poplar Trees Reveals
... Proteomics of Plasma Membranes from Poplar Trees Reveals Tissue Distribution of Transporters, Receptors, and Proteins in Cell ...
... Proteomics of Plasma Membranes from Poplar Trees Reveals Tissue Distribution of Transporters, Receptors, and Proteins in Cell ...
bioinformatics module ii - Tetrahymena Genome Database
... screen for the whole class to follow. I have found that demonstrating two sections at a time gives a good breaking point to not overwhelm them with information (i.e. Demo section I and II; section III and IV; section V and VI; section VII and VIII; and then section IX and X). Section I: Finding the ...
... screen for the whole class to follow. I have found that demonstrating two sections at a time gives a good breaking point to not overwhelm them with information (i.e. Demo section I and II; section III and IV; section V and VI; section VII and VIII; and then section IX and X). Section I: Finding the ...