Functional analysis of the human CDC5L complex
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
Phosphorylation-Dependent Targeting of Tetrahymena HP1 to
... Tetrahymena Hhp1 was originally identified as a member of the HP1 family by virtue of its amino-terminal CD and carboxy-terminal CSD. However, HP1 homologs typically bind histone H3K9-methylated chromatin, which is absent from Tetrahymena macronuclei, where Hhp1 resides (25). Instead, macronuclear ch ...
... Tetrahymena Hhp1 was originally identified as a member of the HP1 family by virtue of its amino-terminal CD and carboxy-terminal CSD. However, HP1 homologs typically bind histone H3K9-methylated chromatin, which is absent from Tetrahymena macronuclei, where Hhp1 resides (25). Instead, macronuclear ch ...
ETs08
... That doesn't sound like much, but if we look at that on a per mole basis it's (1.23 * 10-23 kcal/e-) * 6.022 * 1023 e -/mole = 30.87 kJ/mol = 7.38 kcal/mol which is a reasonable amount of energy on the scale we're accustomed to examining. ...
... That doesn't sound like much, but if we look at that on a per mole basis it's (1.23 * 10-23 kcal/e-) * 6.022 * 1023 e -/mole = 30.87 kJ/mol = 7.38 kcal/mol which is a reasonable amount of energy on the scale we're accustomed to examining. ...
Localization of protein-binding sites within families of proteins
... R2 is 0.19 for the correlation between the overall sequence identity and localization difference), although very similar proteins do tend to have similarly located binding sites and distantly related proteins do not. In addition, the localization difference is not correlated with Ca RMSD (Fig. 3C; R ...
... R2 is 0.19 for the correlation between the overall sequence identity and localization difference), although very similar proteins do tend to have similarly located binding sites and distantly related proteins do not. In addition, the localization difference is not correlated with Ca RMSD (Fig. 3C; R ...
Methods for imaging and detecting modification of proteins
... prostane pathway [19]. Isoketals are highly electrophilic γ-ketoaldehydes that rapidly and covalently modify lysine residues, forming a variety of adducts including lactam, hydroxylactam, imine, and pyrrole species. The enzymatic oxidation of arachidonic acid also results in the formation of reactiv ...
... prostane pathway [19]. Isoketals are highly electrophilic γ-ketoaldehydes that rapidly and covalently modify lysine residues, forming a variety of adducts including lactam, hydroxylactam, imine, and pyrrole species. The enzymatic oxidation of arachidonic acid also results in the formation of reactiv ...
digestion in the pig - The Australian Society of Animal Production
... stomachs, a greater amount of acid and proteolytic enzyme secretory tissue (fundic mucosa) and greater acid and proteolytic enzyme secretory capacities than pigs fed on sow's milk. The different rates of gastric development in pigs fed sow's milk and those fed solid food are probably related to a nu ...
... stomachs, a greater amount of acid and proteolytic enzyme secretory tissue (fundic mucosa) and greater acid and proteolytic enzyme secretory capacities than pigs fed on sow's milk. The different rates of gastric development in pigs fed sow's milk and those fed solid food are probably related to a nu ...
Mechanism of Uptake and Retrograde Axonal Transport of
... adrenergic neurons, budding of small and large catecholaminestoring vesicles from the axonal and terminal ER-like tubular network has aLso been observed (56), and a catecholaminecontaining reticular network has been observed in axons and terminals (56, 61). In neuromuscular junctions, synaptic vesic ...
... adrenergic neurons, budding of small and large catecholaminestoring vesicles from the axonal and terminal ER-like tubular network has aLso been observed (56), and a catecholaminecontaining reticular network has been observed in axons and terminals (56, 61). In neuromuscular junctions, synaptic vesic ...
Chap. 3A Amino Acids, Peptides, and Proteins Topics Amino acids
... into proteins by ribosomal translation. All amino acids contain an a carbon to which typically 4 different substituent groups are attached (Fig. 3-2). These groups are the aamino group, the a-carboxyl group, hydrogen, and the variable R group (side-chain). (Note that in glycine, the R group consists ...
... into proteins by ribosomal translation. All amino acids contain an a carbon to which typically 4 different substituent groups are attached (Fig. 3-2). These groups are the aamino group, the a-carboxyl group, hydrogen, and the variable R group (side-chain). (Note that in glycine, the R group consists ...
Regulation of Maltose Transport and Metabolism in Saccharomyces
... integral membrane protein with high degree of homology with other sugar transporters in eukaryotes. The research on transport kinetics of maltose in mutants which have just one transporter gene (MAL61 or MAL11) showed that those genes encoded high-affinity (low Km) transport system, which is maltose ...
... integral membrane protein with high degree of homology with other sugar transporters in eukaryotes. The research on transport kinetics of maltose in mutants which have just one transporter gene (MAL61 or MAL11) showed that those genes encoded high-affinity (low Km) transport system, which is maltose ...
The distribution of SMN protein complex in human fetal tissues and
... Immunoblot analyses showed that in postnatal skeletal muscle derived from type I SMA patients, the amount of SMN protein was also markedly reduced, but not in a type III patient (17). Interestingly, the observation of a significant reduction in SMN in muscle from type III SMA fetuses suggests that e ...
... Immunoblot analyses showed that in postnatal skeletal muscle derived from type I SMA patients, the amount of SMN protein was also markedly reduced, but not in a type III patient (17). Interestingly, the observation of a significant reduction in SMN in muscle from type III SMA fetuses suggests that e ...
The Arabidopsis Rab5 Homologs Rha1 and Ara7 Localize to the
... role in vacuolar trafficking in plant cells. In this study, we investigated the localization of Rha1 and Ara7, two Arabidopsis proteins that have highly similar amino acid sequence homology to Rab5 in animal cells. Both Ara7 and Rha1 gave a punctate staining pattern and colocalized when transiently ...
... role in vacuolar trafficking in plant cells. In this study, we investigated the localization of Rha1 and Ara7, two Arabidopsis proteins that have highly similar amino acid sequence homology to Rab5 in animal cells. Both Ara7 and Rha1 gave a punctate staining pattern and colocalized when transiently ...
Building proteins from C, coordinates using the dihedral probability
... united atom approach. It is apparent that there is only a small correlation between the backboneenergy and the RMSfit to the crystal structure backbone. The backboneof the crystal structure itself has an energy of 759.8 kcal/mol, higher than 12 of the 20 model conformations. Thisis likely due to lim ...
... united atom approach. It is apparent that there is only a small correlation between the backboneenergy and the RMSfit to the crystal structure backbone. The backboneof the crystal structure itself has an energy of 759.8 kcal/mol, higher than 12 of the 20 model conformations. Thisis likely due to lim ...
Separation and Purification of Angiotensin Converting Enzyme
... Figure 2. ACE-inhibition ratios of goat’s CN hydrolysates by activity. Goat’s milk CN before enzyme treatments showed commercial proteases. Legend: Trypsin (♦), Papain W-40 (◇), a zero hydrolysis ratio. In general, the ACE-inhibitory ratio Protease S (■), Neutrase 1.5 (□) and Pepsin (▲). showed a ra ...
... Figure 2. ACE-inhibition ratios of goat’s CN hydrolysates by activity. Goat’s milk CN before enzyme treatments showed commercial proteases. Legend: Trypsin (♦), Papain W-40 (◇), a zero hydrolysis ratio. In general, the ACE-inhibitory ratio Protease S (■), Neutrase 1.5 (□) and Pepsin (▲). showed a ra ...
Traffic between the plant endoplasmic reticulum and Golgi
... essential residues (i.e. Y717 or K719; [22]) can affect the integrity of the plant TGN or its subdomains, as has been demonstrated in mammalian cells [34,35]. Recent studies have shown that the Golgi apparatus plays a role in transporting some proteins to the chloroplast [36,37], although import rou ...
... essential residues (i.e. Y717 or K719; [22]) can affect the integrity of the plant TGN or its subdomains, as has been demonstrated in mammalian cells [34,35]. Recent studies have shown that the Golgi apparatus plays a role in transporting some proteins to the chloroplast [36,37], although import rou ...
Cysteine 230 Modulates Tumor Necrosis Factor
... Although TRAIL is a TNF family member (10, 11), it has some notable differences compared with TNF-␣ and FasL. First, TRAIL exhibits much stronger apoptotic activity than other TNF family members and is able to kill many cell types without potentiating agents such as actinomycin D or cycloheximide (1 ...
... Although TRAIL is a TNF family member (10, 11), it has some notable differences compared with TNF-␣ and FasL. First, TRAIL exhibits much stronger apoptotic activity than other TNF family members and is able to kill many cell types without potentiating agents such as actinomycin D or cycloheximide (1 ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
... AVTGEXSLNKLQLKDADVKG VDFNVPFDKKD VLNNMAIGSS ADVXVND ...
... AVTGEXSLNKLQLKDADVKG VDFNVPFDKKD VLNNMAIGSS ADVXVND ...
Distribution and phylogeny of the bacterial translational GTPases
... factors that utilize GTP are called translational GTPases (trGTPases). Four large families of trGTPases - IF2/eIF5B, SelB/eIF2γ, EF-Tu/EF-1α and EFG/EF-2 - can be distinguished (Leipe et al. 2002). For each of those families one ancestral gene existed in the last universal common ancestor (LUCA) (Le ...
... factors that utilize GTP are called translational GTPases (trGTPases). Four large families of trGTPases - IF2/eIF5B, SelB/eIF2γ, EF-Tu/EF-1α and EFG/EF-2 - can be distinguished (Leipe et al. 2002). For each of those families one ancestral gene existed in the last universal common ancestor (LUCA) (Le ...
Processing of the Presequence of the Schizosaccharomyces pombe
... the S. pombe iron-sulfur protein into S. cerevisiae mitochondria (Fig. 3B). Again only precursor and mature length protein is detectable, suggesting that the three amino acid motif is not sufficient by itself for sequential two-step processing. To test whether S. pombe mitochondria contain any MPP a ...
... the S. pombe iron-sulfur protein into S. cerevisiae mitochondria (Fig. 3B). Again only precursor and mature length protein is detectable, suggesting that the three amino acid motif is not sufficient by itself for sequential two-step processing. To test whether S. pombe mitochondria contain any MPP a ...
Disulfide formation in plant storage vacuoles permits assembly
... compost and vermiculite under greenhouse conditions at 15°C with a 16 hour light / 8 hour dark cycle. Prior to planting, seeds were imbibed in running water overnight. The development of Ricinus communis seeds is divided into seven stages (A to G) based on size, testa formation and state of hydratio ...
... compost and vermiculite under greenhouse conditions at 15°C with a 16 hour light / 8 hour dark cycle. Prior to planting, seeds were imbibed in running water overnight. The development of Ricinus communis seeds is divided into seven stages (A to G) based on size, testa formation and state of hydratio ...
- Wiley Online Library
... a-D-glucuronidases, acetylxylanesterases, ferulic acid esterases and p-coumaric acid esterases (Table S4). The genes of Xcc-568 found in these different glycosyl hydrolases (GH) or carboxylesterase (CE) families were named to indicate their activity and CAZy family, as previously described for Cellv ...
... a-D-glucuronidases, acetylxylanesterases, ferulic acid esterases and p-coumaric acid esterases (Table S4). The genes of Xcc-568 found in these different glycosyl hydrolases (GH) or carboxylesterase (CE) families were named to indicate their activity and CAZy family, as previously described for Cellv ...