Conditional lethality of a yeast strain expressing human RHOA in place of RHO1 .
... RHOA expression plasmid (pYO714) cannot grow on a YPD plate containing 300 mM CaCl2 (Fig. 3C). Y0C725, which contains the rho) deletion mutation and an integrated human RHOA gene on the chromosome, is also incapable of growin in the presence of high concentrations of CaCl2. Wild-type yeast strains ( ...
... RHOA expression plasmid (pYO714) cannot grow on a YPD plate containing 300 mM CaCl2 (Fig. 3C). Y0C725, which contains the rho) deletion mutation and an integrated human RHOA gene on the chromosome, is also incapable of growin in the presence of high concentrations of CaCl2. Wild-type yeast strains ( ...
Protein secretion and surface display in Gram
... The cell wall peptidoglycan of Gram-positive bacteria functions as a surface organelle for the transport and assembly of proteins that interact with the environment, in particular, the tissues of an infected host. Signal peptide-bearing precursor proteins are secreted across the plasma membrane of G ...
... The cell wall peptidoglycan of Gram-positive bacteria functions as a surface organelle for the transport and assembly of proteins that interact with the environment, in particular, the tissues of an infected host. Signal peptide-bearing precursor proteins are secreted across the plasma membrane of G ...
Supplements - Haiyuan Yu
... Due to performance concerns, it is not possible to convert an entire chromosome without a provided locus to transcripts on the web server (though this can be accomplished through the downloadable command-line tool). Even when a locus is provided, there are many cases in which several transcripts are ...
... Due to performance concerns, it is not possible to convert an entire chromosome without a provided locus to transcripts on the web server (though this can be accomplished through the downloadable command-line tool). Even when a locus is provided, there are many cases in which several transcripts are ...
REVIEWS
... transporters have also been implicated in antibiotic resistance, drug resistance in fungi and parasitic protozoa, and herbicide resistance in plants6. Intriguingly, the bacterial transporter LmrA, when expressed in mammalian cells, confers multidrug resistance indistinguishable from that of mammalia ...
... transporters have also been implicated in antibiotic resistance, drug resistance in fungi and parasitic protozoa, and herbicide resistance in plants6. Intriguingly, the bacterial transporter LmrA, when expressed in mammalian cells, confers multidrug resistance indistinguishable from that of mammalia ...
The Genome-linked Protein of Picornaviruses. VIII. Complete Amino
... (Wimmer, 1982). Moreover, there are four glutaminyl-glycine bonds in the polyprotein that remain uncleaved for unknown reasons. Our results are consistent with other poliovirus proteins whose carboxy-termini have been examined (Emini et al., 1982), in that no carboxy-terminal 'trimming' has been obs ...
... (Wimmer, 1982). Moreover, there are four glutaminyl-glycine bonds in the polyprotein that remain uncleaved for unknown reasons. Our results are consistent with other poliovirus proteins whose carboxy-termini have been examined (Emini et al., 1982), in that no carboxy-terminal 'trimming' has been obs ...
evaluation of the interaction of loratadine and desloratadine with p
... maximum equivalent to only 19% total inhibition (Fig. 1B). Moreover, the IC50 of ⬃43 M was about 4-fold greater than that for L. For DL, the IC50 was ⬃880 times the maximum observed plasma concentration (Cmax) following the recommended dose (5 mg; Banfield et al., 2001a,b). The results described he ...
... maximum equivalent to only 19% total inhibition (Fig. 1B). Moreover, the IC50 of ⬃43 M was about 4-fold greater than that for L. For DL, the IC50 was ⬃880 times the maximum observed plasma concentration (Cmax) following the recommended dose (5 mg; Banfield et al., 2001a,b). The results described he ...
Determinants of Drosophila zw10 protein localization and function
... action, because we did not know the fate of zw10 protein in mitotically arrested cells. In this paper, we describe more detailed analyses, both of the mutant phenotype and of the cell cycle-dependent intracellular distribution of the zw10 protein, which provide clues to the role played by zw10 in mi ...
... action, because we did not know the fate of zw10 protein in mitotically arrested cells. In this paper, we describe more detailed analyses, both of the mutant phenotype and of the cell cycle-dependent intracellular distribution of the zw10 protein, which provide clues to the role played by zw10 in mi ...
The advantages of being small Stockholm University
... energy (Baseman and Tully, 1997). It is also known that some mycoplasmas incorporate host lipids into the membrane (Uemura et al., 1988), which would mimic the host cell and prevent them being discovered by the host immune system. These bacteria thus have a large fraction of specific transporters es ...
... energy (Baseman and Tully, 1997). It is also known that some mycoplasmas incorporate host lipids into the membrane (Uemura et al., 1988), which would mimic the host cell and prevent them being discovered by the host immune system. These bacteria thus have a large fraction of specific transporters es ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... understood [5, 6], but little biochemical and molecular genetic information exists on pollen functions. Some recent studies have tried to break the bottleneck. Transcriptomic analyses of Arabidopsis pollen have identified some transcripts expressed preferentially in pollen [7,8]. Functional classifi ...
... understood [5, 6], but little biochemical and molecular genetic information exists on pollen functions. Some recent studies have tried to break the bottleneck. Transcriptomic analyses of Arabidopsis pollen have identified some transcripts expressed preferentially in pollen [7,8]. Functional classifi ...
The structural biology of the amyloid precursor protein
... 488 I. Coburger et al.: Multi-domain structure of APP Considering the function of the entire APP-protein and its involvement in the aetiology of AD, it is essential to know how these structural and functional segments form and act within the context of the entire protein and within the cellula ...
... 488 I. Coburger et al.: Multi-domain structure of APP Considering the function of the entire APP-protein and its involvement in the aetiology of AD, it is essential to know how these structural and functional segments form and act within the context of the entire protein and within the cellula ...
Bacterial Outer Membrane Vesicles as a Delivery System for
... pathogen-associated molecular patterns, including LPS, lipoprotein, DNA, and RNA. Their interaction with pattern-recognition receptors (PRRs) of host epithelial cells stimulates the production of cytokines and chemokines in the mucosal epithelial surface, the first line of host defense. OMVs release ...
... pathogen-associated molecular patterns, including LPS, lipoprotein, DNA, and RNA. Their interaction with pattern-recognition receptors (PRRs) of host epithelial cells stimulates the production of cytokines and chemokines in the mucosal epithelial surface, the first line of host defense. OMVs release ...
INTRODUCTION - international journal of advances in
... coronary blood flow which then results in hypoxia and accumulation of the toxic cellular metabolites. The accumulation of the lactic acid leads to the decrease in the intracellular pH and increase in intracellular Na+ ([Na+]i) and Ca2+ ([Ca2+]i). The ionic and metabolic disturbance leads to reductio ...
... coronary blood flow which then results in hypoxia and accumulation of the toxic cellular metabolites. The accumulation of the lactic acid leads to the decrease in the intracellular pH and increase in intracellular Na+ ([Na+]i) and Ca2+ ([Ca2+]i). The ionic and metabolic disturbance leads to reductio ...
Amino Acids, Peptides, and Proteins
... is an equal mixture of H3NOCH2OCOOH and H3NOCH2OCOO, the average or net charge on the population of glycine molecules is 0.5 (see the discussion on pp. 80–81). You can use the Henderson-Hasselbalch equation to calculate the exact ratio of charged and uncharged species at equilibrium at various pH ...
... is an equal mixture of H3NOCH2OCOOH and H3NOCH2OCOO, the average or net charge on the population of glycine molecules is 0.5 (see the discussion on pp. 80–81). You can use the Henderson-Hasselbalch equation to calculate the exact ratio of charged and uncharged species at equilibrium at various pH ...
Raven/Johnson Biology 8e Chapter 05 – Answers 1. The description
... C. Answer c is incorrect. The presence of lipid rafts and the amount of cholesterol will influence the efficiency of transport or the organization of the membrane, but they do not determine the specific function. The correct answer is d—type and number of membrane proteins D. Answer d is correct. Th ...
... C. Answer c is incorrect. The presence of lipid rafts and the amount of cholesterol will influence the efficiency of transport or the organization of the membrane, but they do not determine the specific function. The correct answer is d—type and number of membrane proteins D. Answer d is correct. Th ...
Synthetic Peptides as Antigens for Antibody Production
... Expert Protein Analysis System (ExPASy). Other prediction scales include the Turn scales of Pellequer and Westhof (4). These are based on the occurrence of amino acids within turns. The level of correctly predicted antigenicity using this program is high (70%), but the number of predicted antigenic ...
... Expert Protein Analysis System (ExPASy). Other prediction scales include the Turn scales of Pellequer and Westhof (4). These are based on the occurrence of amino acids within turns. The level of correctly predicted antigenicity using this program is high (70%), but the number of predicted antigenic ...
SM-20, EGL-9, and the EGLN Family of Hypoxia
... other stimuli induces the rapid and typically transient expression of so-called ‘immediate early genes’ such as cjun and c-fos. While comparing gene expression in rat vascular smooth muscle cells treated with or without platelet-derived growth factor, Taubman and colleagues (Wax et al., 1994) identi ...
... other stimuli induces the rapid and typically transient expression of so-called ‘immediate early genes’ such as cjun and c-fos. While comparing gene expression in rat vascular smooth muscle cells treated with or without platelet-derived growth factor, Taubman and colleagues (Wax et al., 1994) identi ...
as a PDF
... S. cerevisiae Kin4 consists of only 800 amino acids and the similarity is mainly restricted to the kinase domain. Likewise, S. cerevisiae Snf1 and Kin4 only display similarity in the kinase domain (43% identity). 3.2. Deletion of kfsA In order to determine the function of kfsA in A. nidulans, we del ...
... S. cerevisiae Kin4 consists of only 800 amino acids and the similarity is mainly restricted to the kinase domain. Likewise, S. cerevisiae Snf1 and Kin4 only display similarity in the kinase domain (43% identity). 3.2. Deletion of kfsA In order to determine the function of kfsA in A. nidulans, we del ...
Arginine-glycine-aspartic acid motif incorporated within turnip yellow
... can be expressed and self assemble within plant tissue. ...
... can be expressed and self assemble within plant tissue. ...
Raven/Johnson Biology 8e
... C. Answer c is incorrect. The presence of lipid rafts and the amount of cholesterol will influence the efficiency of transport or the organization of the membrane, but they do not determine the specific function. The correct answer is d—type and number of membrane proteins D. Answer d is correct. Th ...
... C. Answer c is incorrect. The presence of lipid rafts and the amount of cholesterol will influence the efficiency of transport or the organization of the membrane, but they do not determine the specific function. The correct answer is d—type and number of membrane proteins D. Answer d is correct. Th ...
PCTpc201500379rar2_pap_plantcell 1..13
... difference from the wild type (Student’s t test, P < 0.05). In this figure, GMPP activity per milligram protein is shown, and that per gram fresh weight is shown in Supplemental Figure 4. ...
... difference from the wild type (Student’s t test, P < 0.05). In this figure, GMPP activity per milligram protein is shown, and that per gram fresh weight is shown in Supplemental Figure 4. ...
IF-3
... protein synthesis. They are defined by their cyclic association with, and dissociation from, the ribosome. IF factors are involved in prokaryotic initiation. IF-3 is needed for 30S subunits to bind to mRNA and also is responsible for maintaining the 30S subunit in a free form. IF-2 is needed for fMe ...
... protein synthesis. They are defined by their cyclic association with, and dissociation from, the ribosome. IF factors are involved in prokaryotic initiation. IF-3 is needed for 30S subunits to bind to mRNA and also is responsible for maintaining the 30S subunit in a free form. IF-2 is needed for fMe ...
SB401, a pollen-specific protein from Solanum berthaultii
... cells involves actin, there is evidence that KRPs also participate in this process (Romagnoli et al., 2003). Moreover, MTs may have an important role in the guidance of tip growth, as seen in the tip growth of root hair cells of Arabidopsis (Bibikova et al., 1999; Ketelaar et al., 2003). Therefore, ...
... cells involves actin, there is evidence that KRPs also participate in this process (Romagnoli et al., 2003). Moreover, MTs may have an important role in the guidance of tip growth, as seen in the tip growth of root hair cells of Arabidopsis (Bibikova et al., 1999; Ketelaar et al., 2003). Therefore, ...