Smith, E.D., et al., Quantitative evidence for conserved longevity
... Of the 76 genes examined in the RBH set, 11 deletion mutants were found to be significantly long-lived (Table 2, Figure 1, Fig. S1). After controlling for the potential effects of evolutionary conservation, we see a four-fold enrichment for aging-related genes in yeast genes orthologous to worm gene ...
... Of the 76 genes examined in the RBH set, 11 deletion mutants were found to be significantly long-lived (Table 2, Figure 1, Fig. S1). After controlling for the potential effects of evolutionary conservation, we see a four-fold enrichment for aging-related genes in yeast genes orthologous to worm gene ...
INTRODUCTION
... mammalian cells, all maternal in origin. This leads to the concept of heteroplasmy, which typifies many diseases caused by mutations in mtDNA. Heteroplasmy can arise from de-novo mutations of the mtDNA but is much more often inherited by the cells receiving a mixture of normal and mutant mtDNA copie ...
... mammalian cells, all maternal in origin. This leads to the concept of heteroplasmy, which typifies many diseases caused by mutations in mtDNA. Heteroplasmy can arise from de-novo mutations of the mtDNA but is much more often inherited by the cells receiving a mixture of normal and mutant mtDNA copie ...
Computational Biology
... (2) generate initial multiple sequence alignment (MSA) with ClustalW. Delete fragments < 80% of length of query sequence. From these refined MSA, apply 6 different % identity criteria, 6 final MSAs for each test protein. ...
... (2) generate initial multiple sequence alignment (MSA) with ClustalW. Delete fragments < 80% of length of query sequence. From these refined MSA, apply 6 different % identity criteria, 6 final MSAs for each test protein. ...
Role of branched-chain amino acid transporters in Staphylococcus
... predominant BrnQ transporter of isoleucine, valine and leucine. Meanwhile, BrnQ2 is more specific for isoleucine. Furthermore, only the lack of BrnQ1 hinders growth of S. aureus in chemically-defined media with limited concentrations of BCAAs; however, BrnQ2 and BrnQ3 show no such growth phenotype. ...
... predominant BrnQ transporter of isoleucine, valine and leucine. Meanwhile, BrnQ2 is more specific for isoleucine. Furthermore, only the lack of BrnQ1 hinders growth of S. aureus in chemically-defined media with limited concentrations of BCAAs; however, BrnQ2 and BrnQ3 show no such growth phenotype. ...
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr
... thermophilus has a longer CTD tail which is seen to be closer to, and interact more with the a-site tRNA and p-site tRNA in ribosome structures. In order to study the role of the CTD tail of ribosomal protein S13, we created four different strains of E. coli derived from the strains MG1655 and JE28. ...
... thermophilus has a longer CTD tail which is seen to be closer to, and interact more with the a-site tRNA and p-site tRNA in ribosome structures. In order to study the role of the CTD tail of ribosomal protein S13, we created four different strains of E. coli derived from the strains MG1655 and JE28. ...
Cell Host and Microbe
... the reaction converting 2-hydroxyisoflavanones to isoflavones, mainly daidzein and genistein (Akashi et al., 2005). Because isoflavones are important secondary metabolites during plantmicrobe interactions, we selected GmHID1 for further analysis. A search in the soybean genome sequence revealed an o ...
... the reaction converting 2-hydroxyisoflavanones to isoflavones, mainly daidzein and genistein (Akashi et al., 2005). Because isoflavones are important secondary metabolites during plantmicrobe interactions, we selected GmHID1 for further analysis. A search in the soybean genome sequence revealed an o ...
Cytochrome P450 - Spektrum der Wissenschaft
... physically interact in vivo. We adapted this system to our purposes so that it can be used as a screening-system for adrenodoxin mutants and cytochrome P450 mutants to detect proteins with increased interaction to their respective partners. Different adrenodoxin mutants were generated by site-direct ...
... physically interact in vivo. We adapted this system to our purposes so that it can be used as a screening-system for adrenodoxin mutants and cytochrome P450 mutants to detect proteins with increased interaction to their respective partners. Different adrenodoxin mutants were generated by site-direct ...
PDF
... albumin from in and below the basement membrane was generally correlated with negative precipitin tests for these proteins in the foetal blood, but egg albumin was sometimes detected in low titre when there was no evidence for its transmission to the vitelline vessels. However, all conjugates were r ...
... albumin from in and below the basement membrane was generally correlated with negative precipitin tests for these proteins in the foetal blood, but egg albumin was sometimes detected in low titre when there was no evidence for its transmission to the vitelline vessels. However, all conjugates were r ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
167 renal and small intestinal sodium
... and non-mammalian membrane Na+-dependent and Na+-independent transport systems deposited in current databanks. Comparison of the newly cloned renal Na+/Pi symporters revealed that NaPi-1 has only 20 % overall identity to NaPi-2, NaPi-3, NaPi-4 and NaPi-6 which, however, are about 80 % identical to e ...
... and non-mammalian membrane Na+-dependent and Na+-independent transport systems deposited in current databanks. Comparison of the newly cloned renal Na+/Pi symporters revealed that NaPi-1 has only 20 % overall identity to NaPi-2, NaPi-3, NaPi-4 and NaPi-6 which, however, are about 80 % identical to e ...
Differential localization of LTA synthesis proteins and their
... self-interaction was detected and DltD was found to interact with the core LTA synthesis proteins (Fig. 1A and B). LTA depletion in S. aureus results in aberrant positioning of the septa, cell enlargement and eventual cell lysis, indicating a role for this polymer in both cell growth and cell divisi ...
... self-interaction was detected and DltD was found to interact with the core LTA synthesis proteins (Fig. 1A and B). LTA depletion in S. aureus results in aberrant positioning of the septa, cell enlargement and eventual cell lysis, indicating a role for this polymer in both cell growth and cell divisi ...
Protein Folding and Expression
... Examples of successfully expressed proteins can be seen in Table 1. This includes expression of enzymes, antigens, and cytokines. Each protein was produced at a very high level of expression and confirmed to have native biological activity. In addition, proteins from taxonomically distant organisms ...
... Examples of successfully expressed proteins can be seen in Table 1. This includes expression of enzymes, antigens, and cytokines. Each protein was produced at a very high level of expression and confirmed to have native biological activity. In addition, proteins from taxonomically distant organisms ...
Looking towards label-free biomolecular interaction analysis
... channels (<20). Such systems are very useful for performing detailed studies on a small set of analytes, but are not practical for high-throughput applications. An emerging technology within the proteomics arena, which is beginning to build a presence in the marketplace, is planar protein arrays. Th ...
... channels (<20). Such systems are very useful for performing detailed studies on a small set of analytes, but are not practical for high-throughput applications. An emerging technology within the proteomics arena, which is beginning to build a presence in the marketplace, is planar protein arrays. Th ...
Synthetic human prion protein octapeptide repeat binds to the
... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
Chapter 05 Membranes
... have additional molecules attached, such as the positively charged choline ( green) shown. Phosphatidylcholine is a common component of membranes. It is shown in (a) with its chemical formula, (b) as a space-filling model, and (c) as the icon that is used in most of the figures in this ...
... have additional molecules attached, such as the positively charged choline ( green) shown. Phosphatidylcholine is a common component of membranes. It is shown in (a) with its chemical formula, (b) as a space-filling model, and (c) as the icon that is used in most of the figures in this ...
Homology modeling workshop
... fact that although we are far from observing all protein folds, we probably have seen nearly all substructures: • A library of known sub-structures (fragments less than 10 residues) is created. • A range of possible conformations for each fragment in the query protein are selected. ...
... fact that although we are far from observing all protein folds, we probably have seen nearly all substructures: • A library of known sub-structures (fragments less than 10 residues) is created. • A range of possible conformations for each fragment in the query protein are selected. ...
Changes in Cotton Root Proteins Correlated with Resistance to Root
... after inoculation, Fig.1). Thus, as documented for other cotton lines (Shepherd and Huck, 1989; Jenkins et al., 1995; Creech et al., 1995), the resistance genes of isoline 81-249 do not inhibit initial J2 penetration. However, further development of root knot nematode dramatically differed in the su ...
... after inoculation, Fig.1). Thus, as documented for other cotton lines (Shepherd and Huck, 1989; Jenkins et al., 1995; Creech et al., 1995), the resistance genes of isoline 81-249 do not inhibit initial J2 penetration. However, further development of root knot nematode dramatically differed in the su ...
THE THREE-DIMENSIONAL STRUCTURE OF PROTEINS
... COCONOC. X-ray diffraction studies of crystals of amino acids and of simple dipeptides and tripeptides demonstrated that the peptide CON bond is somewhat shorter than the CON bond in a simple amine and that the atoms associated with the peptide bond are coplanar. This indicated a resonance or part ...
... COCONOC. X-ray diffraction studies of crystals of amino acids and of simple dipeptides and tripeptides demonstrated that the peptide CON bond is somewhat shorter than the CON bond in a simple amine and that the atoms associated with the peptide bond are coplanar. This indicated a resonance or part ...
Protein sequence databases
... • Prediction of protein structure is still not possible from sequence alone • Not all mechanisms of protein folding are known • Experimental protein structure determination – is time consuming – is very expensive – is not always possible (protein must be in crystal structure) – results in only one c ...
... • Prediction of protein structure is still not possible from sequence alone • Not all mechanisms of protein folding are known • Experimental protein structure determination – is time consuming – is very expensive – is not always possible (protein must be in crystal structure) – results in only one c ...
Characterizing the complexity of enzymes on the basis of their
... complexity and determine the total number of reactions in vivo, and thus the potential size of the MACiE database, we estimated the number of protein families per EC node on the basis of sequence data (for an upper limit) and structures (for a lower limit). Although this approach does not take into ...
... complexity and determine the total number of reactions in vivo, and thus the potential size of the MACiE database, we estimated the number of protein families per EC node on the basis of sequence data (for an upper limit) and structures (for a lower limit). Although this approach does not take into ...
Dynamics of protein noise can distinguish between alternate
... a slow increase in CV2(t). On the other hand, when expression noise originates from mRNA birth/death fluctuations, then a transcriptional block results in stochastic degradation of mRNAs across the cells, which rapidly increases the proteinnoise levels (Figure 2). In summary, the increase in protein ...
... a slow increase in CV2(t). On the other hand, when expression noise originates from mRNA birth/death fluctuations, then a transcriptional block results in stochastic degradation of mRNAs across the cells, which rapidly increases the proteinnoise levels (Figure 2). In summary, the increase in protein ...
Overview for Electrophoresis and Western Blotting
... insignificant compared to the negative charges provided by the bound detergent so that the SDS-polypeptide complexes have essentially the same negative charge and shape and migrate through the gel strictly according to polypeptide size. The simplicity and speed of this method, plus the fact that onl ...
... insignificant compared to the negative charges provided by the bound detergent so that the SDS-polypeptide complexes have essentially the same negative charge and shape and migrate through the gel strictly according to polypeptide size. The simplicity and speed of this method, plus the fact that onl ...
Constitutive heat shock protein 70 (HSC70) expression in rainbow
... Different genes encode for hsp70 and hsc70 and the coding sequences are continuous for hsp70, whereas for hsc70 the sequences are interrupted with several introns. The human hsc70 (Dworniczak and Mirault, 1987) and hsp70 amino acid sequences (Hunt and Morimoto, 1985) are 81% homologous with differen ...
... Different genes encode for hsp70 and hsc70 and the coding sequences are continuous for hsp70, whereas for hsc70 the sequences are interrupted with several introns. The human hsc70 (Dworniczak and Mirault, 1987) and hsp70 amino acid sequences (Hunt and Morimoto, 1985) are 81% homologous with differen ...
Arbonne-Protein-Shake-Label-Avery
... Arbonne Protein Shake (Chocolate – Vanilla) Add contents to 9 oz. of cold water or almond milk and shake vigorously (add ice and use a blender for a thicker shake). ...
... Arbonne Protein Shake (Chocolate – Vanilla) Add contents to 9 oz. of cold water or almond milk and shake vigorously (add ice and use a blender for a thicker shake). ...