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Constitutive heat shock protein 70 (HSC70) expression in rainbow
Constitutive heat shock protein 70 (HSC70) expression in rainbow

... Different genes encode for hsp70 and hsc70 and the coding sequences are continuous for hsp70, whereas for hsc70 the sequences are interrupted with several introns. The human hsc70 (Dworniczak and Mirault, 1987) and hsp70 amino acid sequences (Hunt and Morimoto, 1985) are 81% homologous with differen ...
Akashi_Gojobori.PNAS02
Akashi_Gojobori.PNAS02

... codon usage were designated as major codons. Major codon usage, MCU, ⫽ (number of major codons)兾(number of major codons ⫹ number of minor codons) in each gene. Only genes with 100 or more codons (excluding start and stop codons) were analyzed. Codons for Lys (AAR) and Glu (GAR) show strong context d ...
Immunoprecipitation protocol
Immunoprecipitation protocol

Keep Your Fingers Off My DNA: Protein–Protein
Keep Your Fingers Off My DNA: Protein–Protein

... Gata1 (FOG1) and its binding partners, the globin transcription factor GATA1 and Transforming Acidic Coiledcoil 3 (TACC3). FOG1 contains a total of nine ZFs (Table 2); however, only four are of the classic C2H2 type. The remaining fingers substitute a conserved cysteine for the final histidine [36]. ...
PDF
PDF

... Shore (1965) in early development of Rana pipiens and R. sylvatica, although some striking changes in these proteins are observed in extracts of homologous organs when tadpoles and frogs of R. catesbeiana are compared (Manwell, 1966). Acid phosphatase, lipase, and N-benzoyl-arginine-naphthylamide ca ...
The Early Interaction of the Outer Membrane Protein PhoE with
The Early Interaction of the Outer Membrane Protein PhoE with

... peripheral subunit of the membrane-embedded SecYEG complex (7). PhoE precursors are then translocated across the IM in a non-native state. When appearing at the periplasmic side of the IM, these polypeptides undergo complex folding processes and have to be prevented from misfolding, aggregating, and ...
TIBS Review - Structural Genomics Consortium
TIBS Review - Structural Genomics Consortium

Metabolic adaptation of Mycobacterium avium subsp
Metabolic adaptation of Mycobacterium avium subsp

... was analysed by DeCyder version 6.0 (Differential Analysis Software, GE Healthcare) using the differential in-gel analysis module. Quantification was applied for filter-confirmed spots with slope .1.4, area ,420 and volume ,130 000. Proteins were considered as differentially expressed if an at least ...
J24077086
J24077086

... Radio-labeled Amino Acids For PET And SPECT Amino acids are important bio-chemical substrates,that play crucial roles in virtually all biological processes. These ionic nutrients serve not only as basic modules of proteins and hormones, but also as neurotransmitters, synaptic modulators, or neurotra ...
Bacteriophage EMS9: Preliminary Genomic Description
Bacteriophage EMS9: Preliminary Genomic Description

... listed first, followed by the position (in base pairs) where the ORF occurs, the DNA strand on which the information is encoded and finally, the nature of the predicted protein is listed. Certain of the ORFs listed encode proteins that are predicted to function in a variety of processes important fo ...
RIBOSOME-INACTIVATING PROTEINS: A Plant Perspective
RIBOSOME-INACTIVATING PROTEINS: A Plant Perspective

... only by tRNATrp from mammalian and avian cells and not by other tRNAs or tRNATrp from yeast (20–23). Given the variety of assays and assay conditions used for RIP analysis, some differences observed in previous studies may be explained by cofactor requirements. It is not known, however, whether the ...
Autophosphorylation Activity of the Arabidopsis Ethylene Receptor
Autophosphorylation Activity of the Arabidopsis Ethylene Receptor

... HCl, 3 M NaOH, or 100 mM Tris-HCl, pH 7.0. Protein bands were cut from the membrane and counted in scintillation fluid. The average for the counts of the acid and base treatments was normalized with respect to the counts for the control treatment (Tris-HCl). Phosphoamino Acid Analysis—Autophosphoryl ...
ARTICLE Functional analysis of mutations in SLC7A9, and genotype
ARTICLE Functional analysis of mutations in SLC7A9, and genotype

... urinary hyperexcretion of cystine and dibasic amino acids. Mutations in SLC3A1, located on chromosome 2p16.3–21 and encoding the bo,+ transporter-related protein rBAT, cause only Type I cystinuria (2,6). The gene causing non-Type I cystinuria was assigned by linkage to chromosome 19q12–13.1 (7,8), a ...
Dynamic properties of a reconstituted myelin sheath
Dynamic properties of a reconstituted myelin sheath

... of PNS myelin [11], where it colocalizes with another myelin-specific protein, P2. The P2 protein, while missing in CNS, represents 1–15% of the total myelin protein in the PNS. MBP is essential for the formation of CNS myelin, able to interact with a wide range of ligands, often polyanionic in natu ...
GuanHongLi (275
GuanHongLi (275

... protein-derived peptides can have ACE-inhibiting properties and thus may be used as a novel functional food for preventing hypertension as well as for therapeutic purposes. In the present study, rice protein was hydrolyzed by protease Alcalase for 2 h and the resulted hydrolysate was determined for ...
Chapter 11 Vitamins and proteins
Chapter 11 Vitamins and proteins

... as coenzymes, which enable a particular enzyme to catalyse a reaction. This will be discussed later in the chapter. Vitamins can be divided into two groups: fat soluble and water soluble. Fat-soluble vitamins include vitamins A, D, E and K. They can be absorbed in the intestines and moved via the ly ...
Molecular paleontology and complexity in the last eukaryotic
Molecular paleontology and complexity in the last eukaryotic

... places all three of the major domains, i.e. bacteria, archaea and eukaryota as monophyletic (top tree). This implies that the eukaryotes branched from the Archaea as a separate and independent lineage, with a stepwise topology, i.e. bacteria emerged first, from which archaea arose and then finally t ...
Viral Antigens Recombinant Proteins
Viral Antigens Recombinant Proteins

... Viral Antigens Recombinant Proteins Large Scale Manufacturing, R&D and Custom Services ...
Glycerolipid transfer for the building of membranes in plant cells.
Glycerolipid transfer for the building of membranes in plant cells.

... other membranes through aqueous phase. Based on the very rapid spontaneous lateral diffusion of lipids within membrane lateral surfaces (0.1 to 1 µm2.s-1; [11]), the lipid distribution in vesicular connected organelles should be homogenous. The fact that this is clearly not the case indicates that v ...
AA139 - Adenium Biotech
AA139 - Adenium Biotech

... Confidential ...
Chapter 3 Part 1
Chapter 3 Part 1

... osmosis, and explain their role in physiological systems. • 3-6 Describe carrier-mediated transport and vesicular transport mechanisms used by cells to facilitate the absorption or removal of specific substances. • 3-7 Explain the origin and significance of the transmembrane potential. ...
Butko M 2012 - Tsien lab Website - University of California San Diego
Butko M 2012 - Tsien lab Website - University of California San Diego

... transfected neurons before BILN-2061 treatment, whereas fluorescence increased over time in 1 µM BILN-2061 (Fig. 1d). Bicuculline, which promotes generation of action potentials in excitatory glutamatergic neurons by antagonizing GABA receptors, increased the rate of fluorescence development (Fig. 1 ...
Holding it all together? Candidate proteins for the plant Golgi matrix
Holding it all together? Candidate proteins for the plant Golgi matrix

... Recently, a family of proteins called golgins that are important for maintaining the integrity of the Golgi apparatus has been identified in animals and yeast (Figure 2). Golgins are large proteins with extensive coiled-coil domains [20–22]. The coiled-coil is a very common protein motif that consis ...
Free fatty acids regulate the uncoupling protein and alternative
Free fatty acids regulate the uncoupling protein and alternative

The enemy within: ricin and plant cells
The enemy within: ricin and plant cells

... plants whose ribosomes are sensitive to their endogenous RIPs, and is presumably a strategy to prevent these toxins from accidentally reaching the cytosol (Bonness et al., 1994). Most of these RIPs are, in fact, targeted into the ER lumen and sorted to the protein storage vacuoles, like ricin, or de ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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