Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes
... cobalamin synthesis (Table 2). (An additional ORF extends from the first nucleotide of the sequence to nucleotide 690; this ORF encodes the C-terminal portion of the pocR regulatory gene [17, 85] and will be discussed below.) Genes assigned to part I of the pathway are assigned cbi designations (syn ...
... cobalamin synthesis (Table 2). (An additional ORF extends from the first nucleotide of the sequence to nucleotide 690; this ORF encodes the C-terminal portion of the pocR regulatory gene [17, 85] and will be discussed below.) Genes assigned to part I of the pathway are assigned cbi designations (syn ...
Computer-Based Design of Novel Protein Structures
... amino acid sequences that could pack well on a target protein backbone. These algorithms have been successful and have been used to stabilize proteins, solubilize membrane proteins, redesign protein-protein interactions, create new enzymes, and design novel protein structures (6, 15, 35, 50, 72). In ...
... amino acid sequences that could pack well on a target protein backbone. These algorithms have been successful and have been used to stabilize proteins, solubilize membrane proteins, redesign protein-protein interactions, create new enzymes, and design novel protein structures (6, 15, 35, 50, 72). In ...
The function of genomes in bioenergetic organelles
... and chloroplasts as free-living eubacteria, or a-proteobacteria, which entered into close inter-dependency with a host cell (Douglas 1998; Delwiche 1999; Lang et al. 1999; McFadden 1999, 2001; Roger 1999; Hackstein et al. 2001; Stoebe & Maier 2002). The genetic systems retained by organelles have ma ...
... and chloroplasts as free-living eubacteria, or a-proteobacteria, which entered into close inter-dependency with a host cell (Douglas 1998; Delwiche 1999; Lang et al. 1999; McFadden 1999, 2001; Roger 1999; Hackstein et al. 2001; Stoebe & Maier 2002). The genetic systems retained by organelles have ma ...
ABSTRACT Title of Document:
... almost the entire nuo operon (with half of nuoA missing), which encodes for the 14 subunit Mtb type I NADH dehydrogenase complex (NDH-1), along with other known-function and predicted-function genes. To identify which genes conferred the anti-apoptotic phenotype, a series of deletion mutants was cre ...
... almost the entire nuo operon (with half of nuoA missing), which encodes for the 14 subunit Mtb type I NADH dehydrogenase complex (NDH-1), along with other known-function and predicted-function genes. To identify which genes conferred the anti-apoptotic phenotype, a series of deletion mutants was cre ...
Nitrite transport to the chloroplast in Chlamydomonas reinhardtii
... Putative NAR1;2 and NAR1;5 would have transit peptides of 70 and 74 residues, respectively, and would share with NAR1;1 a high proportion of Ala (25–45%) and Pro (30–50%) in the last 20 amino acids (Fig. 3). The Chlamydomonas ESTs present in the GenBank databases do not have a single EST correspondi ...
... Putative NAR1;2 and NAR1;5 would have transit peptides of 70 and 74 residues, respectively, and would share with NAR1;1 a high proportion of Ala (25–45%) and Pro (30–50%) in the last 20 amino acids (Fig. 3). The Chlamydomonas ESTs present in the GenBank databases do not have a single EST correspondi ...
User`s Guide - Structural Bioinformatics Group
... flowchart, model building. But, just before performing this last task, we will manually check the alignment to pinpoint potential misaligned regions. Indeed, scrolling the alignment till the C-terminal region (approximately near ASP 130 of the query sequence) we notice four consecutive ASP residues ...
... flowchart, model building. But, just before performing this last task, we will manually check the alignment to pinpoint potential misaligned regions. Indeed, scrolling the alignment till the C-terminal region (approximately near ASP 130 of the query sequence) we notice four consecutive ASP residues ...
Full-Text PDF
... can be optimized and many of the problems associated with modifying existing organisms can be avoided. In simpler protocells, protective cellular pathways can be eliminated and resistance to product toxicity engineered—for instance by exclusion of target molecules. The formation of unwanted byproduc ...
... can be optimized and many of the problems associated with modifying existing organisms can be avoided. In simpler protocells, protective cellular pathways can be eliminated and resistance to product toxicity engineered—for instance by exclusion of target molecules. The formation of unwanted byproduc ...
SHORT COMMUNICATION DETERMINATION OF AMINO ACIDS
... flowering period of different plants from May to September. It was also found that in same causes RJ composition, mainly proteins, depends on storage conditions of the product [11]. As expected, sometimes RJ cannot be sold immediately after harvesting. For this reason, it is very important RJ to be ...
... flowering period of different plants from May to September. It was also found that in same causes RJ composition, mainly proteins, depends on storage conditions of the product [11]. As expected, sometimes RJ cannot be sold immediately after harvesting. For this reason, it is very important RJ to be ...
Defining the impact on yeast ATP synthase of two pathogenic
... necessarily mean that mitochondrial ATP synthesis was not compromised. Indeed as previously shown through the analysis of numerous yeast ATP synthase mutants, the activity of this enzyme needs to be decreased by at least 80% to see an obvious respiratory growth defect, which indicates that ATP synth ...
... necessarily mean that mitochondrial ATP synthesis was not compromised. Indeed as previously shown through the analysis of numerous yeast ATP synthase mutants, the activity of this enzyme needs to be decreased by at least 80% to see an obvious respiratory growth defect, which indicates that ATP synth ...
5 | structure and function of plasma membranes
... has evolved somewhat over time, but it still best accounts for the structure and functions of the plasma membrane as we now understand them. The fluid mosaic model describes the structure of the plasma membrane as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrat ...
... has evolved somewhat over time, but it still best accounts for the structure and functions of the plasma membrane as we now understand them. The fluid mosaic model describes the structure of the plasma membrane as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrat ...
Effect of surface hydrophobicity distribution on retention
... retention in HIC. The effect of the surface properties on protein retention has also been broached in a study about ion-exchange chromatography, where the effect of surface charge distribution of proteins was investigated [12]. Recently, the effect of surface hydrophobicity distribution of proteins ...
... retention in HIC. The effect of the surface properties on protein retention has also been broached in a study about ion-exchange chromatography, where the effect of surface charge distribution of proteins was investigated [12]. Recently, the effect of surface hydrophobicity distribution of proteins ...
pH Homeostasis in Lactic Acid Bacteria
... prevent, in part, overacidification of the finished product. In the production of yogurt, for example, fermentation lowers the pH of the milk from 6.5 to between 4.0 and 4.5, and the milk coagulates. Unrestricted growth and fermentation in yogurt (or postacidification) by the lactic culture may resu ...
... prevent, in part, overacidification of the finished product. In the production of yogurt, for example, fermentation lowers the pH of the milk from 6.5 to between 4.0 and 4.5, and the milk coagulates. Unrestricted growth and fermentation in yogurt (or postacidification) by the lactic culture may resu ...
pdf
... Only tRNA’s with the amino acid esterified to the 3´ hydroxyl group of the 3´ terminal adenosine residue are utilized by the translation apparatus. The tRNA’s charged by the Class I aminoacyl tRNA synthetases must be modified, the amino acid must be moved from the 2´ hydroxyl to the 3´ hydroxyl gro ...
... Only tRNA’s with the amino acid esterified to the 3´ hydroxyl group of the 3´ terminal adenosine residue are utilized by the translation apparatus. The tRNA’s charged by the Class I aminoacyl tRNA synthetases must be modified, the amino acid must be moved from the 2´ hydroxyl to the 3´ hydroxyl gro ...
PDF Datastream - Brown Digital Repository
... preliminary method development on gel-assisted digestion and solid phase extraction of glycopeptides. I am also grateful to former undergraduates Seth Levin, Helen M. Johnson at Brown University for their assistance in biological sample preparations. I would like to thank many members in Professor B ...
... preliminary method development on gel-assisted digestion and solid phase extraction of glycopeptides. I am also grateful to former undergraduates Seth Levin, Helen M. Johnson at Brown University for their assistance in biological sample preparations. I would like to thank many members in Professor B ...
Document
... Example of conserved regions (PPID family) - 1 CSA_PPIASE (cyclophilin-type peptydil-prolyl cis-trans isomerase) (domain) - 3 TPR repeats (tetratrico peptide repeat). - 1 active site ...
... Example of conserved regions (PPID family) - 1 CSA_PPIASE (cyclophilin-type peptydil-prolyl cis-trans isomerase) (domain) - 3 TPR repeats (tetratrico peptide repeat). - 1 active site ...
Precursor of human adenovirus core polypeptide Mu targets the
... Mu–EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu–EGFP (but not Mu–EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes ...
... Mu–EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu–EGFP (but not Mu–EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes ...
Lecture 1 – Classification - LCQB
... The domains within a fold are further classified into superfamilies. Domains belonging to the same superfamily have structural evidence to support a common evolutionary ancestor but may not have detectable sequence homology. Ex: Globin-like and Alpha-helical ferredoxin are the two superfamilies of t ...
... The domains within a fold are further classified into superfamilies. Domains belonging to the same superfamily have structural evidence to support a common evolutionary ancestor but may not have detectable sequence homology. Ex: Globin-like and Alpha-helical ferredoxin are the two superfamilies of t ...
Relationship Between the Occurrence of Cysteine in Proteins and
... Cysteine is unique among coded amino acids because it contains a reactive sulph-hydryl group. Therefore, two cysteine residues may form a cystine (disulfide link) between various parts of the same protein or between two separate polypeptide chains. The formation of a disulfide link is coupled with t ...
... Cysteine is unique among coded amino acids because it contains a reactive sulph-hydryl group. Therefore, two cysteine residues may form a cystine (disulfide link) between various parts of the same protein or between two separate polypeptide chains. The formation of a disulfide link is coupled with t ...
Hereditary Myopathy with Lactic Acidosis
... investigate the underlying disease-mechanisms. In paper I we identified a diseasecritical region on chromosome 12; a region containing 16 genes. One of the genes coded for the Fe-S cluster assembly protein ISCU and an intronic base pair substitution (g.7044G>C) was identified in the last intron of t ...
... investigate the underlying disease-mechanisms. In paper I we identified a diseasecritical region on chromosome 12; a region containing 16 genes. One of the genes coded for the Fe-S cluster assembly protein ISCU and an intronic base pair substitution (g.7044G>C) was identified in the last intron of t ...
The Invention of Proteomic Code and mRNA
... accidental and I started looking for an additional “code in the code” which might explain the redundancy of codons and give some rational explanation what the excess information in nucleic acids might be used for. There was an idea published in early 80-s [31-35] suggesting that specifically interac ...
... accidental and I started looking for an additional “code in the code” which might explain the redundancy of codons and give some rational explanation what the excess information in nucleic acids might be used for. There was an idea published in early 80-s [31-35] suggesting that specifically interac ...
Protein Applications in Sports Nutrition—Part I
... inadequate amounts or ratios of one or more of the 9 essential amino acids. Typically, if a protein source comes from an animal, such as beef, pork, fish, egg, milk (or dairy products), and poultry, such as chicken or turkey, it is considered a complete source of protein. Proteins derived from plant ...
... inadequate amounts or ratios of one or more of the 9 essential amino acids. Typically, if a protein source comes from an animal, such as beef, pork, fish, egg, milk (or dairy products), and poultry, such as chicken or turkey, it is considered a complete source of protein. Proteins derived from plant ...