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ABA control of plant macroelement membrane transport systems in
ABA control of plant macroelement membrane transport systems in

... ABCG40 transporter (Fig. 2b). Because the Arabidopsis ABAdeficit mutant plants display a more severe phenotype to drought stress than abcg25 and abcg40 plants, additional transporters with redundant functions might also be involved in the transport of ABA. Alternatively, passive ABA transport by pH ...
BI25M1
BI25M1

... to make agricultural fertilizer, which needs: an iron catalyst, 5000C and 300 atmospheres.] ...
A new type of plant chitinase containing LysM domains from a fern
A new type of plant chitinase containing LysM domains from a fern

... et al. 1993; Graham and Sticklen 1994). However, an endogenous substrate for plant chitinases has not yet been found. In the absence of an endogenous substrate, plant chitinases may be involved in the interaction between plants and microbes, which produce chitin and chitin-related compounds. One of ...
The extraction of collagen protein from pigskin
The extraction of collagen protein from pigskin

... was found to be in between the other two enzymes. Because water is the solvent, it has the mildest reaction conditions. From this study, the order of hydrolysis ability was proved to be: pancreatin > papain > pepsin. The triple helix structure of the collagen protein obtained from pepsin hydrolysis ...
Subcellular Localization and Functional Analysis
Subcellular Localization and Functional Analysis

... drastically altered leaf morphology and reduced plant size, providing experimental evidence for an important role of RabE GTPases in regulating plant growth. RabE down-regulation did not affect plant susceptibility to pathogenic P. syringae bacteria; conversely, expression of the constitutively acti ...
Association of CAD, a multifunctional protein involved in pyrimidine
Association of CAD, a multifunctional protein involved in pyrimidine

Molecular Microbiology
Molecular Microbiology

... effectors (T3SEs) are essential virulence factors in Gram-negative bacteria. During the arms race, plants have evolved resistance (R) genes to detect specific T3SEs and activate defence responses. However, this immunity can be efficiently defeated by the pathogens through effector evolution. HopZ1 o ...
Invited Chapter One
Invited Chapter One

... other) that they each constitute a distinct subfamily. Interestingly, the threemember nematode subfamily contains proteins that cooperate in the same pathway but have distinct functions. Each of these proteins influences dauer formation, an alternative third-stage larva specialized for survival and ...
Amino acid residues that determine functional specificity of NADP
Amino acid residues that determine functional specificity of NADP

Evolution and Function of the Plant Cell Wall
Evolution and Function of the Plant Cell Wall

... clade, proteins from either moss or spike moss or both are found in four of the seven GAUT subclades: the ones containing AtGAUT1 to AtGAUT3, AtGAUT8 and AtGAUT9, AtGAUT10 and AtGAUT11, and AtGAUT12 to AtGAUT15. Five moss proteins and one spike moss protein are found in the GATL clade, with the form ...
Understanding Hyaluronan-Protein Interactions
Understanding Hyaluronan-Protein Interactions

... glycosaminoglycan.8 This is also likely to be the case for CD44, for which two arginines (Arg41 and Arg78) play a critical role in ligand binding.6,9 In addition, two tyrosines (Tyr42, Tyr79) are essential for CD44 functional activity (Figure 11); mutation of Tyr79 to phenylalanine destroys function ...
RalA ACTIVATION ASSAY BIOCHEM KIT
RalA ACTIVATION ASSAY BIOCHEM KIT

... starved. Serum starvation will inactivate cellular RalA and lead to a much greater response to a given RalA activator. C. Timing and Intensity of RalA Activation Upon stimulation, RalA proteins are generally activated very rapidly and transiently, maximal activation ranges from 30 s to 30 min and de ...
Protein S-nitrosylation in photosynthetic organisms: A
Protein S-nitrosylation in photosynthetic organisms: A

... Stomata movements are regulated by abscisic acid (ABA) and this hormone is known to interact with NO in many processes and to stimulate its accumulation during stomata regulation [25]. In Arabidopsis mutants lacking the two NR isoforms, neither ABA-induced NO production nor ABA-induced stomata closu ...
Testis-specific TAF homologs collaborate to control a
Testis-specific TAF homologs collaborate to control a

... Tissue-specific TAFs program spermatogenesis 5299 Qiagen OneStep RT-PCR kit. For northern blots and in situ hybridization, the mia probe was generated from a DNA fragment corresponding to the first 1331 bp of the mia cDNA, the nht probe was generated from a plasmid containing a PCR product correspo ...
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in

... gel electrophoresis followed by N-terminal (Edman) sequencing and/or amino acid analysis. However, such methods are considerably more time-consuming and in some cases are not capable of separating individual peptides because of their low resolution. Consequently, high-resolution MALDI/TOF analysis c ...
Intrinsically Disordered Protein - Center for Data Analytics and
Intrinsically Disordered Protein - Center for Data Analytics and

... The molten globule was initially discovered as an equilibrium structure observed in studies on protein denaturation. Later this protein form was proposed to exist as a transient intermediate during the kinetics of protein folding.53 Indeed, several ingenious studies have provided evidence for this p ...
Changes in gene expression associated with acclimation to constant
Changes in gene expression associated with acclimation to constant

Are viruses a source of new protein folds for organisms? Virosphere
Are viruses a source of new protein folds for organisms? Virosphere

... Viruses have had proportionally fewer of their structures experimentally determined than cellular organisms To detect an SF domain there must be a representative that has had its structure determined experimentally (usually by X-ray crystallography or NMR). Below we show that the virosphere structur ...
[7] Semisynthesis of Proteins Containing Selenocysteine
[7] Semisynthesis of Proteins Containing Selenocysteine

... Strategies for Synthesis of Proteins Containing Selenocysteine The methods we describe herein for the synthesis of selenocysteine-containing proteins are variations of the peptide ligation method first described by Wieland et al. g and later developed by Kent and co-workers.9 This method makes use o ...
The transcriptome of proteostasis perturbations reveal non
The transcriptome of proteostasis perturbations reveal non

... and ribosome biogenesis. Although a recent report (23) has shown that Rqc2 is essential in mounting a functional HSR using HSE-GFP based reporter system, we find that HSR is among the most upregulated category in rqc2Δ. This could be due to the fact that we have taken the top 40 genes upregulated up ...
Cloning and sequence analysis of cnaA gene encoding the catalytic
Cloning and sequence analysis of cnaA gene encoding the catalytic

Food derived from glufosinate ammonium
Food derived from glufosinate ammonium

... The pat gene is derived from the soil micro-organism Streptomyces viridochromogenes strain Tu494. It codes for the enzyme phosphinothricin acetyl transferase (PAT), which modifies and inactivates the herbicide glufosinate ammonium (Strauch et al, 1988). The pat gene is often used as a selectable mar ...
Predicting the Secondary Structure of Globular Proteins Using
Predicting the Secondary Structure of Globular Proteins Using

... in the protein sequence is likely to produce significantly better results for non-homologous proteins. The performance of our method of homologous proteins is much better than for non-homologous proteins, but is not as good as simply assuming that homologous sequences have identical structures. ...
Basis of preclinical studies_Biochemistry_Practicals_LI
Basis of preclinical studies_Biochemistry_Practicals_LI

... 4. Be able to discuss the significance of alterations of protein amount in blood serum. 1) Biuret reaction Principle: The biuret reaction is a method that can be used to determine the amount of soluble protein in a solution. The biuret reagent (copper sulfate in a strong base) reacts with peptide bo ...
The CamSol Method of Rational Design of Protein Mutants with
The CamSol Method of Rational Design of Protein Mutants with

... seven gammabody mutants with a predicted increased solubility with respect to the wild-type form (Table 1 and Table S1). No particular rule was applied in selecting these seven gammabody variants. We decided to include in the set the wild type (WT in Table 1), the variant predicted to be the most so ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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