A Novel Plant Major Intrinsic Protein in Physcomitrella patens Most
... from the clone PPAS020308, were found. These ESTs encode MIP peptides that are very unlike previously known plant MIPs. Sequencing of PPAS020308 revealed a 1,602-bp-long cDNA insert with an open reading frame of 1,113 bp. Alignments with other MIPs support that this open reading frame contains the c ...
... from the clone PPAS020308, were found. These ESTs encode MIP peptides that are very unlike previously known plant MIPs. Sequencing of PPAS020308 revealed a 1,602-bp-long cDNA insert with an open reading frame of 1,113 bp. Alignments with other MIPs support that this open reading frame contains the c ...
Lecture 32: Protein (Part-I)
... middle. Based on these calculations, he has constructed Ramchandran plot to define the region of allowed rotation for amino acids present in a protein structure and proposed to use this to validate the 3-D structure of a protein model (Figure 32.1, C). Amino Acids: As discussed earlier, proteins are ...
... middle. Based on these calculations, he has constructed Ramchandran plot to define the region of allowed rotation for amino acids present in a protein structure and proposed to use this to validate the 3-D structure of a protein model (Figure 32.1, C). Amino Acids: As discussed earlier, proteins are ...
paper describing HSSP
... values for single residues, pairs of residues, short oligopeptides or short sequence patterns. Both kinds of methods are severely limited by the size of the database in which one performs searches or from which one derives structural preferences. For example, in order to have on the average 5 occurr ...
... values for single residues, pairs of residues, short oligopeptides or short sequence patterns. Both kinds of methods are severely limited by the size of the database in which one performs searches or from which one derives structural preferences. For example, in order to have on the average 5 occurr ...
Agrobacterium tumefaciens and Plant Cell - igem
... allows the expression of the virulence machinery across a broad range of host plants and, conceivably, different cell types within the plant. VirA and signal perception. VirA is a dimeric membrane-bound protein and is found in this state even in the absence of signals (Pan et al. 1993). Several doma ...
... allows the expression of the virulence machinery across a broad range of host plants and, conceivably, different cell types within the plant. VirA and signal perception. VirA is a dimeric membrane-bound protein and is found in this state even in the absence of signals (Pan et al. 1993). Several doma ...
Metabolite transport across the peroxisomal membrane
... membrane protein of 47 kDa (PMP47). Although its function was unclear at the time, it was soon recognized on the basis of primary sequence homology to be a member of the MCF (mitochondrial carrier family) (SLC25) [28]. Since then, orthologues have been identified in A. thaliana (PMP38) [29], humans ...
... membrane protein of 47 kDa (PMP47). Although its function was unclear at the time, it was soon recognized on the basis of primary sequence homology to be a member of the MCF (mitochondrial carrier family) (SLC25) [28]. Since then, orthologues have been identified in A. thaliana (PMP38) [29], humans ...
Transamination and asymmetry in glutamate transport across the
... maintain a relatively low plasma concentration of the potentially toxic amino acid glutamate (the concentration of glutamate in the plasma of Rana c a t e s b e i a n a is about 90 pM; Boyd, 1976), while the high intraceilular level of glutamate will favour transamination to alanine. Since the basol ...
... maintain a relatively low plasma concentration of the potentially toxic amino acid glutamate (the concentration of glutamate in the plasma of Rana c a t e s b e i a n a is about 90 pM; Boyd, 1976), while the high intraceilular level of glutamate will favour transamination to alanine. Since the basol ...
Molecular Characterization of Calymmin, a Novel Notochord Sheath
... did not show significant homology to any of the known proteins present in the databases, nor were any putative conserved domains found. However, further analysis by recursive PSI-BLAST searches (Corpet et al., 2000) of the novel protein identified herein, which we named Calymmin, identified four con ...
... did not show significant homology to any of the known proteins present in the databases, nor were any putative conserved domains found. However, further analysis by recursive PSI-BLAST searches (Corpet et al., 2000) of the novel protein identified herein, which we named Calymmin, identified four con ...
Dual-topology membrane proteins Escherichia coli Susanna Seppälä
... although there are pathogenic variants that may cause disease. Since its discovery by Theodor Escherich in 1885, E. coli has become a popular workhorse in laboratories worldwide, the main reasons being that the cells are easy to cultivate, and that their genetic content is easily manipulated. Many f ...
... although there are pathogenic variants that may cause disease. Since its discovery by Theodor Escherich in 1885, E. coli has become a popular workhorse in laboratories worldwide, the main reasons being that the cells are easy to cultivate, and that their genetic content is easily manipulated. Many f ...
The paradox of elongation factor 4: highly conserved, yet of no
... G’. However, EF4 contains a unique CTD (C-terminal domain) that is not found in any other trGTPase [43,48,49] (Figures 2A and 2B). Domain I (also called the G domain) and domain II are conserved in both structure and sequence in all trGTPases [49,50], suggesting that these two domains work together ...
... G’. However, EF4 contains a unique CTD (C-terminal domain) that is not found in any other trGTPase [43,48,49] (Figures 2A and 2B). Domain I (also called the G domain) and domain II are conserved in both structure and sequence in all trGTPases [49,50], suggesting that these two domains work together ...
REGULATORY MECHANISMS OF CELLULAR RESPIRATION I
... of uranyl phosphate appeared after centrifugation for 1 hour at 3,000 I~.P.M. In absence of protein, the UO~IPO4 precipitate appeared immediately. At p H 3.8, in hippurate buffer, the albumin-U complex was more strongly associated than at p H 7.3, as shown b y the ultraflltration experiments in Tabl ...
... of uranyl phosphate appeared after centrifugation for 1 hour at 3,000 I~.P.M. In absence of protein, the UO~IPO4 precipitate appeared immediately. At p H 3.8, in hippurate buffer, the albumin-U complex was more strongly associated than at p H 7.3, as shown b y the ultraflltration experiments in Tabl ...
Derivation and testing of pair potentials for protein folding. When is
... also ignores the presence of secondary structural elements in the native state. Ideally, one would like a reference state having both features, but where the interaction between pairs of residues is nonspecific. One path toward the creation of an approximate reference state that possesses such prope ...
... also ignores the presence of secondary structural elements in the native state. Ideally, one would like a reference state having both features, but where the interaction between pairs of residues is nonspecific. One path toward the creation of an approximate reference state that possesses such prope ...
Sulphur and nitrogen regulation of the protease
... responds to the availability of sulphur to the fungal cell, we used a modified B05.10 B. cinerea strain (strain T3) carrying a transcriptional fusion (pACP1-GUS) able to drive an intracellular production of b-glucuronidase (GUS) that parallels that of the endogenous ACP1 (Rolland et al., 2003). The ...
... responds to the availability of sulphur to the fungal cell, we used a modified B05.10 B. cinerea strain (strain T3) carrying a transcriptional fusion (pACP1-GUS) able to drive an intracellular production of b-glucuronidase (GUS) that parallels that of the endogenous ACP1 (Rolland et al., 2003). The ...
Plasmodium falciparum enolase - Tata Institute of Fundamental
... in high titers in Japanese and Thai P. falciparum patient sera and use of yeast enolase for immunodiagnostic purposes [15]. The activity of enolase in parasite-infected red blood cells increases 15-fold [16]. The gene for P. falciparum (strain K1) enolase (Pfen) has been cloned and characterized [ ...
... in high titers in Japanese and Thai P. falciparum patient sera and use of yeast enolase for immunodiagnostic purposes [15]. The activity of enolase in parasite-infected red blood cells increases 15-fold [16]. The gene for P. falciparum (strain K1) enolase (Pfen) has been cloned and characterized [ ...
Lecture 5 PP
... century) a relationship between genes and protein production Garrod studied patients who had defects in their ability to metabolize certain compounds ...
... century) a relationship between genes and protein production Garrod studied patients who had defects in their ability to metabolize certain compounds ...
Topological studies suggest that the pathway of the protons through
... TID-reactive residues in F0• The labeling profilein Fig. 58 was obtaincd after reaction with Neurospora crassa mitochondria in the presence of an oligomycin concentration 10-fold higher than necessary for maximal inhibition. The identical group of residues was TID-reactive in the presence and in the ...
... TID-reactive residues in F0• The labeling profilein Fig. 58 was obtaincd after reaction with Neurospora crassa mitochondria in the presence of an oligomycin concentration 10-fold higher than necessary for maximal inhibition. The identical group of residues was TID-reactive in the presence and in the ...
Guanine Nucleotides Modulate the Effects of Brefeldin A in
... (Donaldson et al., 1990). The ll0-kD protein also redistributed to the cytosol, in a manner indistinguishable from BFA-treated cells, when cellular ATP levels were depleted by treatment with sodium azide and 2-deoxyglucose (Donaldson et al., 1990). The ability of ATP levels to regulate the distribut ...
... (Donaldson et al., 1990). The ll0-kD protein also redistributed to the cytosol, in a manner indistinguishable from BFA-treated cells, when cellular ATP levels were depleted by treatment with sodium azide and 2-deoxyglucose (Donaldson et al., 1990). The ability of ATP levels to regulate the distribut ...
Green Fluorescent Protein
... • 20 fold enhancement consistent with 20 fold increase of GFP protein levels • GFP mutants can fluoresce different colors and be used simultaneously to monitor independent events in cells • Some GFP mutants exhibit more rapid formation of fluorophore ...
... • 20 fold enhancement consistent with 20 fold increase of GFP protein levels • GFP mutants can fluoresce different colors and be used simultaneously to monitor independent events in cells • Some GFP mutants exhibit more rapid formation of fluorophore ...
A Novel Plant Major Intrinsic Protein in
... from the clone PPAS020308, were found. These ESTs encode MIP peptides that are very unlike previously known plant MIPs. Sequencing of PPAS020308 revealed a 1,602-bp-long cDNA insert with an open reading frame of 1,113 bp. Alignments with other MIPs support that this open reading frame contains the c ...
... from the clone PPAS020308, were found. These ESTs encode MIP peptides that are very unlike previously known plant MIPs. Sequencing of PPAS020308 revealed a 1,602-bp-long cDNA insert with an open reading frame of 1,113 bp. Alignments with other MIPs support that this open reading frame contains the c ...
Structure of a Plasmodium yoelii gene
... for 2h at 42 °C. Hybridizations were done under the same conditions using a 1 ;
... for 2h at 42 °C. Hybridizations were done under the same conditions using a 1 ;
Glucose transporters - Cambridge University Press
... dietary glucose with fructose. The magnitude of this disorder, by the mutation of a single gene, would indicate that alternative lumenal glucose transport does not occur to any significant extent. The relevance of SGLT3 intestinal expression needs to be addressed and suggests that care should be tak ...
... dietary glucose with fructose. The magnitude of this disorder, by the mutation of a single gene, would indicate that alternative lumenal glucose transport does not occur to any significant extent. The relevance of SGLT3 intestinal expression needs to be addressed and suggests that care should be tak ...
soy protein concentrate - US Soybean Export Council
... the processing of mature soybeans. High quality soybeans are selected and cleaned as the first step in soy product processing. The clean soybeans are dehulled, then the oil is extracted for cooking, biodiesel and other uses. The residue, consisting of defatted white flakes, can then be ground to mak ...
... the processing of mature soybeans. High quality soybeans are selected and cleaned as the first step in soy product processing. The clean soybeans are dehulled, then the oil is extracted for cooking, biodiesel and other uses. The residue, consisting of defatted white flakes, can then be ground to mak ...
Steven`s project - The University of Texas at Dallas
... Using the downloaded software The downloaded software is much more difficult to use and does not contain user friendly GU interfaces. Whenever possible a novice user should opt for the on line version as described above. However, if you intend to repack proteins quite regularly, or need to repack i ...
... Using the downloaded software The downloaded software is much more difficult to use and does not contain user friendly GU interfaces. Whenever possible a novice user should opt for the on line version as described above. However, if you intend to repack proteins quite regularly, or need to repack i ...
Amassin, an olfactomedin protein, mediates the massive
... demonstrated by others (Donnellon, 1938; Bookhout and Greenburg, 1940; Davidson, 1953). Clotting was also found to depend on the formation and presence of disulfide bonds (Boolootian and Giese, 1959; Bertheussen and Seijelid, 1978). More recent work showed that clotting was accompanied by a massive ...
... demonstrated by others (Donnellon, 1938; Bookhout and Greenburg, 1940; Davidson, 1953). Clotting was also found to depend on the formation and presence of disulfide bonds (Boolootian and Giese, 1959; Bertheussen and Seijelid, 1978). More recent work showed that clotting was accompanied by a massive ...
figure 1 - Proceedings of the Royal Society B
... GO:0008610). The species were chosen based on the amount of data available in the database, and A. thaliana was the only plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. P ...
... GO:0008610). The species were chosen based on the amount of data available in the database, and A. thaliana was the only plant species for which none of the categories listed was empty. Different functional groups contain overlap, as some proteins may participate in more than one functional class. P ...
Sulfur Metabolism in Escherichia coli and Related Bacteria: Facts
... hydrogen sulfide, H2S. These systems are often poorly known. However, one knows that, for example, thiosulfate can be an excellent sulfur source in E. coli (Sirko et al., 1995). In the same way we do not know much about the use of organic sulfur sources. Biosynthesis of Cysteine The reaction catalyz ...
... hydrogen sulfide, H2S. These systems are often poorly known. However, one knows that, for example, thiosulfate can be an excellent sulfur source in E. coli (Sirko et al., 1995). In the same way we do not know much about the use of organic sulfur sources. Biosynthesis of Cysteine The reaction catalyz ...