Characterization of the Distal Polyadenylation Site of the ß
... minigene products is then tested by Northern blot analysis or RTPCR of total RNA prepared from transfected cells. In order to study the molecular mechanisms of polyadenylation of the mouse Add2 gene, we constructed a chimeric minigene by replacing the alpha-globin natural PAS in an ampicillin-resist ...
... minigene products is then tested by Northern blot analysis or RTPCR of total RNA prepared from transfected cells. In order to study the molecular mechanisms of polyadenylation of the mouse Add2 gene, we constructed a chimeric minigene by replacing the alpha-globin natural PAS in an ampicillin-resist ...
Sensitivity of plant mitochondrial terminal oxidases to the lipid
... from Arabidopsis cell cultures and measured activities of both the Aox and Cox pathways of respiration. Mitochondria were incubated for 20 min with increasing concentrations of HNE (0– 700 µM), prior to measuring oxygen consumption with succinate as substrate in the presence of ADP, to maximize elec ...
... from Arabidopsis cell cultures and measured activities of both the Aox and Cox pathways of respiration. Mitochondria were incubated for 20 min with increasing concentrations of HNE (0– 700 µM), prior to measuring oxygen consumption with succinate as substrate in the presence of ADP, to maximize elec ...
Interdependence, Reflexivity, Fidelity, Impedance Matching
... By the time protein folding organizes amino acid side chains into a functional active site, genetic information has been irreversibly transformed. Any molecular machine charged with reversing translation by unfolding, then “reading” the sequence of a protein would require shuttling ...
... By the time protein folding organizes amino acid side chains into a functional active site, genetic information has been irreversibly transformed. Any molecular machine charged with reversing translation by unfolding, then “reading” the sequence of a protein would require shuttling ...
Structure, function and regulation of pyruvate carboxylase
... Role of PC in insulin signalling in pancreatic islets Glucose is a potent stimulator of insulin secretion from βpancreatic cells when extracellular levels are greater than 3 mM. Secretion of insulin in response to a high concentration of glucose results in the rapid uptake of glucose by pancreatic β ...
... Role of PC in insulin signalling in pancreatic islets Glucose is a potent stimulator of insulin secretion from βpancreatic cells when extracellular levels are greater than 3 mM. Secretion of insulin in response to a high concentration of glucose results in the rapid uptake of glucose by pancreatic β ...
Interdependence, Reflexivity, Fidelity, Impedance Matching
... By the time protein folding organizes amino acid side chains into a functional active site, genetic information has been irreversibly transformed. Any molecular machine charged with reversing translation by unfolding, then “reading” the sequence of a protein would require shuttling ...
... By the time protein folding organizes amino acid side chains into a functional active site, genetic information has been irreversibly transformed. Any molecular machine charged with reversing translation by unfolding, then “reading” the sequence of a protein would require shuttling ...
Globins in Nonvertebrate Species: Dispersal by Horizontal Gene
... John Czeluzniak, 5 Morris Goodman, 5 Mark Blaxter,ll and Serge Vinogradofl *Department of Biochemistry, University of Antwerp; TDepartment of Morphology, Systematics and Ecology, University of Ghent; *Department of Radiology and Enrico Fermi Institute, University of Chicago; SDepartment of Anatomy, ...
... John Czeluzniak, 5 Morris Goodman, 5 Mark Blaxter,ll and Serge Vinogradofl *Department of Biochemistry, University of Antwerp; TDepartment of Morphology, Systematics and Ecology, University of Ghent; *Department of Radiology and Enrico Fermi Institute, University of Chicago; SDepartment of Anatomy, ...
F-actin Sequesters Elongation Factor from Interaction with
... al. (1976), except that the ammonium sulphate precipitation step was omitted. Briefly, 10 I~M of Dictyostelium EF-lct and 100 p.M of [32P]-~,-GTP were incubated in column buffer containing 20 mM Pipes, 5 mM MgC12, 5 mM 13-mercaptoethanol, 100 mM NH4CI, and 15% glycerol at pH 6.5 or 7.0 for 30 rain a ...
... al. (1976), except that the ammonium sulphate precipitation step was omitted. Briefly, 10 I~M of Dictyostelium EF-lct and 100 p.M of [32P]-~,-GTP were incubated in column buffer containing 20 mM Pipes, 5 mM MgC12, 5 mM 13-mercaptoethanol, 100 mM NH4CI, and 15% glycerol at pH 6.5 or 7.0 for 30 rain a ...
Dynamic balance between vesicle transport and
... right set of membrane proteins, but also causes an accumulation of membrane proteins involved in anterograde transport at the GC and of membrane proteins involved in retrograde transport at the TGN. To ensure availability of the membrane proteins for the next set of budding vesicles, they need to be ...
... right set of membrane proteins, but also causes an accumulation of membrane proteins involved in anterograde transport at the GC and of membrane proteins involved in retrograde transport at the TGN. To ensure availability of the membrane proteins for the next set of budding vesicles, they need to be ...
Post-transcriptional regulation of auxin transport proteins: cellular
... In Arabidopsis thaliana, the 21 members of ABCB subgroup exhibit both distinct and overlapping expression patterns throughout all stages of plant growth and development (Blakeslee et al., 2005b). The best characterized members of Arabidopsis ABCB proteins are the auxin transporters ABCB1, ABCB4, and ...
... In Arabidopsis thaliana, the 21 members of ABCB subgroup exhibit both distinct and overlapping expression patterns throughout all stages of plant growth and development (Blakeslee et al., 2005b). The best characterized members of Arabidopsis ABCB proteins are the auxin transporters ABCB1, ABCB4, and ...
Short-term adenosine monophosphate–activated protein kinase
... (omitted for the measurement of nonspecific activity), and 5 mmol/L adenosine triphosphate (omitted for the measurement of nonspecific activity), pH 7.0, were used. 2.6. Statistical analysis All data are expressed as the means ± SE. To estimate the time course of the protein expressions and hexokina ...
... (omitted for the measurement of nonspecific activity), and 5 mmol/L adenosine triphosphate (omitted for the measurement of nonspecific activity), pH 7.0, were used. 2.6. Statistical analysis All data are expressed as the means ± SE. To estimate the time course of the protein expressions and hexokina ...
irm_ch20
... 20.66 –C=O and –N–H 20.67 In a beta-pleated sheet structure, two fully extended protein chain segments are held together by hydrogen bonds. The beta-pleated sheet may be intermolecular when two different peptide chains are aligned parallel to each other, or intramolecular when a single molecule fold ...
... 20.66 –C=O and –N–H 20.67 In a beta-pleated sheet structure, two fully extended protein chain segments are held together by hydrogen bonds. The beta-pleated sheet may be intermolecular when two different peptide chains are aligned parallel to each other, or intramolecular when a single molecule fold ...
article in press - Biochemistry
... NP006244) and g- (GenBank: NP997626) regulatory subunits. Mammals express several isoforms of each subunit, and different combinations produce enzymes with differing tissue and subcellular localization, as well as altered sensitivity to AMP (see Hardie, 2003 for a review of the known differences). H ...
... NP006244) and g- (GenBank: NP997626) regulatory subunits. Mammals express several isoforms of each subunit, and different combinations produce enzymes with differing tissue and subcellular localization, as well as altered sensitivity to AMP (see Hardie, 2003 for a review of the known differences). H ...
12 Molecular Visualization of an Enzyme, Acetylcholinesterase
... The visualization program Chimera will be introduced to you by using the structure of the multi-drug resistance (MDR) efflux pump protein. This is the protein that is up-regulated by a riboswitch in response to antibiotics like tetracycline. The protein then pumps the antibiotics out of the bacteria ...
... The visualization program Chimera will be introduced to you by using the structure of the multi-drug resistance (MDR) efflux pump protein. This is the protein that is up-regulated by a riboswitch in response to antibiotics like tetracycline. The protein then pumps the antibiotics out of the bacteria ...
The Influence of Ammonium Permease Activity and
... in samples from which the cells had been removed by centrifugation, using the reducing sugar determination of Somogyi & Nelson (1944). The ammonium concentration of the samples was determined using the urea/ammonia kit supplied by Boehringer Mannheim. Determinationof ammonium pool size. A sample of ...
... in samples from which the cells had been removed by centrifugation, using the reducing sugar determination of Somogyi & Nelson (1944). The ammonium concentration of the samples was determined using the urea/ammonia kit supplied by Boehringer Mannheim. Determinationof ammonium pool size. A sample of ...
Production and analysis protein and phosphor mineral of a high
... foods is cassava. Cassava is rich of carbohydrate, are used in some of food as like as chips and alcoholic beverage. Benefit of cassava is increasing by processing of it until materials, as like as tapioca flour (Radiyati, 1990). In Indonesia we can meet some food with tapioca flour as major materia ...
... foods is cassava. Cassava is rich of carbohydrate, are used in some of food as like as chips and alcoholic beverage. Benefit of cassava is increasing by processing of it until materials, as like as tapioca flour (Radiyati, 1990). In Indonesia we can meet some food with tapioca flour as major materia ...
and Schizosaccharomyces pombe
... of the TCA cycle (Barnett and Kornberg 1960). Previous studies have shown that L-malate can be utilised by Candida utilis (CaÂssio and LeaÄo 1993), Candida sphaerica (CoÃrte-Real et al. 1989), Hansenula anomala (CoÃrteReal and LeaÄo 1990) and Kluyveromyces marxianus (Queiros et al. 1998) as their on ...
... of the TCA cycle (Barnett and Kornberg 1960). Previous studies have shown that L-malate can be utilised by Candida utilis (CaÂssio and LeaÄo 1993), Candida sphaerica (CoÃrte-Real et al. 1989), Hansenula anomala (CoÃrteReal and LeaÄo 1990) and Kluyveromyces marxianus (Queiros et al. 1998) as their on ...
Evolution of b-type cytochromes in prokaryotes
... of two clusters; hypothetical "I" (khaki) sequences are dispersed, although most group with hypothetical "E" and "J" sequences (as might be expected); all "J" sequences (light blue) end up in one of three clusters interspersed with "I" sequences, except for two which end up in the ...
... of two clusters; hypothetical "I" (khaki) sequences are dispersed, although most group with hypothetical "E" and "J" sequences (as might be expected); all "J" sequences (light blue) end up in one of three clusters interspersed with "I" sequences, except for two which end up in the ...
Safety Assessment of Soy Proteins and Peptides as Used in
... 71 kDa) and β (ca 50 kDa). The 11S globulin is a hexamer, and is made up of five different subunits, each of which consists of an acidic subunit A (ca 35 kDa) and a basic subunit B (ca 20 kDa), linked by a disulfide bond. The 11S globulin was found to dissociate into 2S, 3S or 7S forms in solutions ...
... 71 kDa) and β (ca 50 kDa). The 11S globulin is a hexamer, and is made up of five different subunits, each of which consists of an acidic subunit A (ca 35 kDa) and a basic subunit B (ca 20 kDa), linked by a disulfide bond. The 11S globulin was found to dissociate into 2S, 3S or 7S forms in solutions ...
ecoli lysis - KSU Faculty Member websites
... Enterotoxigenic Escherichia coli (ETEC) is an enteric pathogen that causes choleralike diarrhea in humans and animals. ETEC secretes a heat-labile enterotoxin (LT), which resembles cholera toxin, but the actual mechanism of LT secretion is presently unknown. We have identified a previously unrecogni ...
... Enterotoxigenic Escherichia coli (ETEC) is an enteric pathogen that causes choleralike diarrhea in humans and animals. ETEC secretes a heat-labile enterotoxin (LT), which resembles cholera toxin, but the actual mechanism of LT secretion is presently unknown. We have identified a previously unrecogni ...
Colicins produced by the Escherichia fergusonii strains closely
... 3.3. E. fergusonii EF36 pDS503, a result of Tn7 insertion into pColE1 from strain EF36 (pColE1-EF36), encoded a cea gene that was most closely related to cea of Shigella sonnei pKY-1 plasmid (97.2% identity; [5]), di¡ering by 16 nucleotide changes and insertion of 7 bp and 1-bp deletion. However, th ...
... 3.3. E. fergusonii EF36 pDS503, a result of Tn7 insertion into pColE1 from strain EF36 (pColE1-EF36), encoded a cea gene that was most closely related to cea of Shigella sonnei pKY-1 plasmid (97.2% identity; [5]), di¡ering by 16 nucleotide changes and insertion of 7 bp and 1-bp deletion. However, th ...
Yeast
... and yeast extract 5 g/l at pH 5·6, acording to the method described by Ochoa et al., 1995. The culture was made in sterile 60 l Nalgene carboys filled up to half their volume with sterile culture medium, adding 10 ml of 10% FG10 antifoam agent (Dow Corning) and 2 ml/l of a 5% chlorine dioxide soluti ...
... and yeast extract 5 g/l at pH 5·6, acording to the method described by Ochoa et al., 1995. The culture was made in sterile 60 l Nalgene carboys filled up to half their volume with sterile culture medium, adding 10 ml of 10% FG10 antifoam agent (Dow Corning) and 2 ml/l of a 5% chlorine dioxide soluti ...
University of Groningen Transport processes in penicillin
... a slower rate of molecular evolution [263]. However, in addition to pcbC all other $-lactam biosynthetic genes that have been cloned and characterized show similar DNA and amino acid sequence conservation. Several other aspects also indicate horizontal gene transfer [188]: i) in nature only a limite ...
... a slower rate of molecular evolution [263]. However, in addition to pcbC all other $-lactam biosynthetic genes that have been cloned and characterized show similar DNA and amino acid sequence conservation. Several other aspects also indicate horizontal gene transfer [188]: i) in nature only a limite ...