ProteoChem`s Current Catalogue.
... Protein crosslinking reagents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes. The atoms separating a crosslinker’s reactive groups, and eventually the conjugated proteins, form the “spacer ...
... Protein crosslinking reagents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes. The atoms separating a crosslinker’s reactive groups, and eventually the conjugated proteins, form the “spacer ...
Selected Reaction Monitoring (SRM) to determine protein
... confirming the reduction in abundance of the encoded protein will often aid the link between genotype and phenotype. However, measuring specific protein abundance is particularly difficult in plant research where only a limited number of antibodies are available. This problem is enhanced when studyi ...
... confirming the reduction in abundance of the encoded protein will often aid the link between genotype and phenotype. However, measuring specific protein abundance is particularly difficult in plant research where only a limited number of antibodies are available. This problem is enhanced when studyi ...
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus
... suggesting that it may be involved in electron interaction within the active site as has been reported for the cysteine protease SpeB (30). It should be noted that tryptophan 194 is not a conserved residue of sortase. Effect of Alanine Substitutions of Sortase on in Vivo Surface Protein Anchoring in ...
... suggesting that it may be involved in electron interaction within the active site as has been reported for the cysteine protease SpeB (30). It should be noted that tryptophan 194 is not a conserved residue of sortase. Effect of Alanine Substitutions of Sortase on in Vivo Surface Protein Anchoring in ...
The Role of Nucleoside Diphosphate Kinase in Plant Mitochondria
... Nucleoside Diphosphate Kinase (NDPK) catalyses the transfer of a phosphate from nucleoside triphosphates to a nucleoside diphosphate, is ubiquitously found in all organisms from bacteria to human. It was discovered that the genes nm23 and awd, which encode NDPKs are involved in tumour metastasis and ...
... Nucleoside Diphosphate Kinase (NDPK) catalyses the transfer of a phosphate from nucleoside triphosphates to a nucleoside diphosphate, is ubiquitously found in all organisms from bacteria to human. It was discovered that the genes nm23 and awd, which encode NDPKs are involved in tumour metastasis and ...
Regulation of phospholipase D activity, membrane targeting and
... [16]. Taken together, these results strongly suggested that a binding interaction between the PLD enzymes and PtdIns(4,5)P2 is important for stimulation of catalysis by this lipid. PLD1, PLD2 and Spo14p have a common multidomain structure. Their catalytic core contains tandem HKD or PLD catalytic do ...
... [16]. Taken together, these results strongly suggested that a binding interaction between the PLD enzymes and PtdIns(4,5)P2 is important for stimulation of catalysis by this lipid. PLD1, PLD2 and Spo14p have a common multidomain structure. Their catalytic core contains tandem HKD or PLD catalytic do ...
Amino Acids, Peptides, and Proteins
... subunits linked in precise functional polymers. Sugars linked together become energy stores and structural fibers; nucleotides strung together in DNA or RNA provide the blueprint for an entire organism; and aggregated lipids form membrane bilayers. Chapter 13 unifies the discussion of biomolecule fu ...
... subunits linked in precise functional polymers. Sugars linked together become energy stores and structural fibers; nucleotides strung together in DNA or RNA provide the blueprint for an entire organism; and aggregated lipids form membrane bilayers. Chapter 13 unifies the discussion of biomolecule fu ...
Biological Networks Underlying Abiotic Stress Tolerance in
... physical properties (changes in the fluidity of phospholipid molecules in plasma membrane bilayer under low or high temperatures) or chemical composition of the ambient environment (e.g., dehydration stress or salinity leading to a decrease of water potential or an increase in Na+ concentration in a ...
... physical properties (changes in the fluidity of phospholipid molecules in plasma membrane bilayer under low or high temperatures) or chemical composition of the ambient environment (e.g., dehydration stress or salinity leading to a decrease of water potential or an increase in Na+ concentration in a ...
design of lupin seeds lactic acid fermentation – changes of
... Fermentation of lupin was carried out 24 h at 30°C for L. sakei, 32°C for P. acidilactici and 35°C for P. pentosaceus. At the end of fermentation, the colony number in the fermented lupin was on the average of 7.28 log10 CFU g1, and the final moisture content of SSF products was on the average of 4 ...
... Fermentation of lupin was carried out 24 h at 30°C for L. sakei, 32°C for P. acidilactici and 35°C for P. pentosaceus. At the end of fermentation, the colony number in the fermented lupin was on the average of 7.28 log10 CFU g1, and the final moisture content of SSF products was on the average of 4 ...
Antibiotic use and abuse: A threat to mitochondria and chloroplasts
... tetracyclines (Fig. 1B). Tetracyclines occupy the A-site of the bacterial 30S ribosomal subunit and inhibit bacterial polypeptide synthesis by sterically blocking the recruitment of the aminoacyl-tRNA to the bacterial ribosome [43, 44, 52]. Ribosomes are biological machines composed of RNAs and prot ...
... tetracyclines (Fig. 1B). Tetracyclines occupy the A-site of the bacterial 30S ribosomal subunit and inhibit bacterial polypeptide synthesis by sterically blocking the recruitment of the aminoacyl-tRNA to the bacterial ribosome [43, 44, 52]. Ribosomes are biological machines composed of RNAs and prot ...
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... topologicalforms ofprionprotein in the endoplasmic reticulum membrane and theirrole inthe pathophysiologicalprocess of the disease. But still prion diseases continue 1o raise many unanswerable questions to be investigated. One of the unanswerable questions is prion muhiplication. Many prion multipli ...
... topologicalforms ofprionprotein in the endoplasmic reticulum membrane and theirrole inthe pathophysiologicalprocess of the disease. But still prion diseases continue 1o raise many unanswerable questions to be investigated. One of the unanswerable questions is prion muhiplication. Many prion multipli ...
Emergence of robust growth laws from optimal regulation of
... features of the underlying regulation that give rise to the growth laws remain unclear. Specifically, what molecular mechanisms are responsible for the allocation of cellular resources that guarantee optimal growth irrespective of the nutrient environment and how do simple empirical relationships em ...
... features of the underlying regulation that give rise to the growth laws remain unclear. Specifically, what molecular mechanisms are responsible for the allocation of cellular resources that guarantee optimal growth irrespective of the nutrient environment and how do simple empirical relationships em ...
Isolation and Characterization of Foaming Proteins of
... precipitation and ion-exchange chromatography on diethylaminoethylcellulose. Because the content of foaming proteins correlated well with head formation of many samples of beer, these foaming proteins seem to be responsible for beer foaming. Foaming proteins consisted of three fractions with molecul ...
... precipitation and ion-exchange chromatography on diethylaminoethylcellulose. Because the content of foaming proteins correlated well with head formation of many samples of beer, these foaming proteins seem to be responsible for beer foaming. Foaming proteins consisted of three fractions with molecul ...
GLYCOLIPID ANCHORING OF CELL SURFACE PROTEINS Mark C
... the action of an endogenous enzyme,35,37 which removed myristic acid from the VSG38 while generating the 'cross reacting determinant' (CRD), the only common immunogenic structure observed between different VSG variants.39,40 The CRD itself is associated with a complex oligosaccharide that was attach ...
... the action of an endogenous enzyme,35,37 which removed myristic acid from the VSG38 while generating the 'cross reacting determinant' (CRD), the only common immunogenic structure observed between different VSG variants.39,40 The CRD itself is associated with a complex oligosaccharide that was attach ...
Towards the construction of Escherichia coli cell
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
Articulins and epiplasmins - Journal of Cell Science
... epiplasm generally reveals many different molecular mass bands, with a pronounced interspecific variation in banding pattern, even among closely related species that are nearly identical morphologically (see, for example, Williams et al., 1984). Polyclonal and monoclonal antibodies (mAbs) directed a ...
... epiplasm generally reveals many different molecular mass bands, with a pronounced interspecific variation in banding pattern, even among closely related species that are nearly identical morphologically (see, for example, Williams et al., 1984). Polyclonal and monoclonal antibodies (mAbs) directed a ...
Two Separate Functions Are Encoded by the Carboxyl
... To localize MCH1 in mammalian cells, Rat-6 fibroblasts were seeded at a density of 5 3 104 on pre-sterilized coverslips. After 24 h in medium containing 10% bovine calf serum, the coverslips were washed (31) with PBS (phosphate-buffered saline) and fixed in a solution of paraformaldehyde (3%) and su ...
... To localize MCH1 in mammalian cells, Rat-6 fibroblasts were seeded at a density of 5 3 104 on pre-sterilized coverslips. After 24 h in medium containing 10% bovine calf serum, the coverslips were washed (31) with PBS (phosphate-buffered saline) and fixed in a solution of paraformaldehyde (3%) and su ...
A1071 GM Canola MON88302 AppR SD1
... Monsanto Australia Limited has submitted an application to FSANZ to vary Standard 1.5.2 – Food produced using Gene Technology – in the Australia New Zealand Food Standards Code (the Code) to include food from a new genetically modified (GM) canola line MON88302 (OECD Unique Identifier MON-88302-9). ...
... Monsanto Australia Limited has submitted an application to FSANZ to vary Standard 1.5.2 – Food produced using Gene Technology – in the Australia New Zealand Food Standards Code (the Code) to include food from a new genetically modified (GM) canola line MON88302 (OECD Unique Identifier MON-88302-9). ...
The application of Tet repressor in prokaryotic gene regulation and
... expression to various levels. Thereby the effect of a potential drug on disease symptoms can be revealed or possible side-effects originating from the physiological response of the pathogen to a drug, or the survival properties of the pathogen under treatment conditions can be monitored. Regulation ...
... expression to various levels. Thereby the effect of a potential drug on disease symptoms can be revealed or possible side-effects originating from the physiological response of the pathogen to a drug, or the survival properties of the pathogen under treatment conditions can be monitored. Regulation ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... Rpd3, impairing the ability of Hairy to repress transcription (15). It is not yet known whether Hairy is able to simultaneously recruit all of these cofactors to any promoter target. ...
... Rpd3, impairing the ability of Hairy to repress transcription (15). It is not yet known whether Hairy is able to simultaneously recruit all of these cofactors to any promoter target. ...
PCtpc201500393rar1_pap_plantcell 1..22
... and posttranslational levels (Bayle et al., 2011). Thus, under Pi deprivation conditions, expression of PHT1 genes is increased in a PHOSPHATE STARVATION RESPONSE1 (PHR1)-dependent manner (Rubio et al., 2001). Transcription factor PHR1 and homolog proteins play a fundamental role during plant adapta ...
... and posttranslational levels (Bayle et al., 2011). Thus, under Pi deprivation conditions, expression of PHT1 genes is increased in a PHOSPHATE STARVATION RESPONSE1 (PHR1)-dependent manner (Rubio et al., 2001). Transcription factor PHR1 and homolog proteins play a fundamental role during plant adapta ...
BCH 305
... amino acids. Because amino acids are one of the components of human skin secretions, the ninhydrin reaction was once used extensively by law enforcement and forensic personnel for fingerprint detection. (Fingerprints as old as 15 years can be successfully identified using the ninhydrin ...
... amino acids. Because amino acids are one of the components of human skin secretions, the ninhydrin reaction was once used extensively by law enforcement and forensic personnel for fingerprint detection. (Fingerprints as old as 15 years can be successfully identified using the ninhydrin ...
Proteolytic processing in the secretory pathway of Aspergillus niger
... pathway and the vacuolar pathway (Nakano, 2004). There are at least two protein sorting pathways leading from the Golgi to the vacuole, and there are indications that more than one pathway exists for protein sorting to the plasma membrane. Protein transport from one organelle to another is mediated ...
... pathway and the vacuolar pathway (Nakano, 2004). There are at least two protein sorting pathways leading from the Golgi to the vacuole, and there are indications that more than one pathway exists for protein sorting to the plasma membrane. Protein transport from one organelle to another is mediated ...
Multivariate Modeling of Proteins Related to
... The prevalence of chronic trapezius myalgia is high in women with high exposure to awkward working positions, repetitive movements and movements with high precision demands. The mechanisms behind chronic trapezius myalgia are not fully understood. The purpose of this study was to explore the differe ...
... The prevalence of chronic trapezius myalgia is high in women with high exposure to awkward working positions, repetitive movements and movements with high precision demands. The mechanisms behind chronic trapezius myalgia are not fully understood. The purpose of this study was to explore the differe ...