Download Quiz 2 Review Sheet

Exam 2 Review Sheet
AP Biology
USE THIS SHEET AFTER YOU HAVE STUDIED AS A METHOD OF EVALUATING WHAT YOU
STILL NEED TO WORK ON.
Exam 2 will cover:
Chapter 4 – ALL
Chapter 5 – ALL
Make sure you look at ALL the questions in both chapters, bold words, headings
and figures, the PowerPoint, and this review sheet.
I posted a macromolecule review chart online.
1. Explain on the molecular level what happens to a crystal composed of glucose when
you throw it in water (don’t just say that it dissolves).
2. Compare and contrast organic compounds and hydrocarbons. Why are all
hydrocarbons organic, but not all organic molecules are hydrocarbons?
3. Be able to identify/circle the carbon skeleton of a hydrocarbon.
4. Compare and contrast structural, geometric and enantiomeric isomers. Be able to draw
examples of each.
5. Explain how you can determine by looking at a molecule if it will have an
enantiomeric isomer in terms of an asymmetric carbon.
6. We said the all amino acids are “L” and all sugar are “D”. What does this refer to?
7. What is meant by “cis” and “trans” in terms of geometric isomers? Draw a pair of cis
and trans isomers. Why is the double bond necessary? What property makes it necessary?
8. Be able to convert a skeletal formula into the corresponding structural formula.
9. Be able to draw a methane molecule three-dimensionally using the wedge-and-dash
projection learned in class.
10. Compare tetrahedral to planar and draw examples of each.
11. Describe the formation of early Earth in terms of the Great Bombardment,
atmosphere formation, ocean formation, etc…
12. How are elements larger than lithium formed?
13. Describe the Miller-Urey experiment and explain the results/conclusions.
14. Define teratogen and give an example.
15. Know the functional group table in the textbook. Be able to draw the functional
groups.
16. Identify the functional groups in the molecule below (there are three):
17. I can ask you a questions similar to: draw an organic molecule that contain a
sulfhydryl group and is acidic.
18. Compare and contrast aldehydes to ketones. Why are these NOT considered
functional groups, but rather a type of compound? What is the functional group found in
each of these? Which class of macromolecule contains both types of compounds?
19. Which types of compounds usually end in –ol? What functional group is involved?
20. Describe in detail why carboxylic acids are considered acids (You will need to
discuss electronegativity differences between atoms). What class of macromolecules
contain this functional group?
21. Compare and contrast the carbonyl to the carboxyl group. Which two functional
groups add to be a carboxyl? Reminder: if the group is a ccarboxy, it is not also a
carbonyl and a hydroxyl (You cannot circle the C=O in question 6 and say it’s a
carbonyl. Why?)
22. Compare hydrophobic and hydrophilic. What makes something hydrophobic or
hydrophilic? Describe how this works on the MOLECULAR LEVEL. Don’t just say
nonpolar or polar… Speak in terms of electronegativity and the electromagnetic force.
23. What is meant by a macromolecule? List each and its general function in organisms.
24. Compare monomers to polymers. Know the terms dimer, trimer, tetramer, pentamer,
etc…
25. Draw ATP as discussed in class and explain why this molecule is like a loaded gun.
What is the role (function) of ATP in the cell? There are two obvious roles.
26. Explain the reactions in detail used to build (anabolic, synthesis) and break (catabolic,
digest) the macromolecules. – you should be able to draw these using spheres.
27. Compare and contrast monosaccharides, disaccharides and polysaccharides. In
addition, be able to give examples of each. All together these are called what?
28. Be able to draw a chart indicating the various combinations of monosaccharides and
the respective disaccharide that forms (maltose, sucrose, lactose).
29. Be able to draw the ring structure of glucose.
30. Describe how two glucose molecules join together and the type of linkage that is
formed (Figure 3.5). You should be able to draw this chemical reaction.
31. Describe the difference between alpha and beta glucose, and explain why these two
different forms exist.
32. What does the word “carbohydrate” mean, literally and why do we use this word to
describe the kinds of molecules that we do?
33. Discuss what is meant by the empirical formula [CH2O]n
34. Look at the structure of ANY monosaccharide. What functional group is attached to
every carbon except one? What functional group is that other carbon a part of?
35. If I told you to draw C6H12O6, what would your response be to me…
36. Make sure you can identify different glycosidic linkages when looking at
disaccharides. Ex. Beta 1,4 vs alpha 1,6 etc…
37. Compare and contrast the three polysaccharides discussed in class. Include their
structures, why they have the same or different structures, where they are found in the
world, their functions in organisms, how structure relates to function in this case, etc…
38. Cellulose is considered “fiber”. Like when the commercials on TV say that Cheerios
is high in “fiber”. What is fiber? Why is cellulose called or at least part of what we call
“fiber”? What is the benefit of insoluble fiber if we cannot digest it?
39. Why are most animals not able to hydrolyze cellulose, but can hydrolyze glycogen
and starch when all three are just polymers of glucose? How are some animals able to
hydrolyze cellulose?
40. Explain what happens to plant starch when you eat it (like if you eat a potatoe).
41. What is the monomer of the protein, how many are there, what makes each of these
different, how are they similar and how are they grouped (the three groups)? Can you
draw any two of them yet and connect them via dehydration synthesis (condensation
reaction)?...you need to be able to do this.
42. Where does the “amino acid” get its name from?
43. Why do I feel that proteins are the most important class of macromolecules (they are
all important of course)?
44. Compare an amino acid, dipeptide, polypeptide, and protein.
45. Describe how a dipeptide is formed (Figure 3.13). What do we call the bond that links
them? How is this analogous to what happens with carbohydrates?
46. Describe the three groups of amino acids. Which group has the acidic and basic ones?
47. What determines the structure of a particular protein? Explain why. Of course, the
structure then determines the…
48. Explain why a change in the DNA might lead to problems.
49. Describe in general terms what happens to a polypeptide (or any polymer for that
matter) when you eat it. What happens to it, what do you do with it, etc…
50. Describe the four levels of protein structure. I could ask another 15 questions about
each level… Know all the details we went over in class like when the side chains come
into play or when only the backbone is involved, hydrophobic vs hydrophilic side chains,
etc…
51. Explain why these levels of structure have little to do with actual protein folding in
the cytoplasm.
52. Explain, starting with a gene, how a protein is formed. Make sure you include items
like mRNA, tRNA, ribosome, nuclear pore, RNA polymerase, amino acids, nucleus,
etc… Basically, describe the central dogma.
53. Be able to identify the various secondary structure elements in a protein and circle
them if presented with a ribbon diagram.
54. Explain how a ribbon diagram is generated. What are you looking at when you view a
ribbon diagram of a protein – (these are the colorful diagrams with sheets shown as
arrows and helices are cartoon-like spirals)?
55. Compare and contrast a polypeptide to a protein. When are they the same and when
would they be different?
56. What is meant by a subunit in a protein?
57. Explain what is meant by denature and renature. How and why do proteins denature?
You must be very specific. You cannot just say a lower pH than the protein evolved in
will cause it to denature…the question is WHY.
58. Know the functions of proteins. Know examples like hemoglobin, myosin, insulin,
insulin receptor, ATP synthase, photosystem II, antibodies, HIV protease, etc… and
under what category they would fall.
59. How small is a protein relative to the size of a cell? (use the analogy) How many
hemoglobin proteins are packed into a single red blood cell? How many red blood cells
do you have on average? How many oxygens can your blood carry if it were completely
saturated with oxygen?
60. Why are some people considered to be lactose intolerant? What does this mean and
why is it a problem?
61. Are glycogen, starch and cellulose the only three polysaccharides found in
organisms? Explain.
62. What is the fate of glucose? Basically, what do organisms use glucose monomers for?
Why do we store them? Where do humans store them?
63. If there is too much glucose in your blood, how does your body handle this? Where
does it end up? Where might this surge in glucose have come from?
64. For every polymer we eat (disaccharides, polysaccharides, polypeptides, etc…), we
need to have a ___________________ somewhere in our digestive tract to hydrolyze it.
This may seem obvious, but we eat cells (unless you are eating processed food, which
case the cells have already been destroyed and broken apart) of other organisms. There
we eat macromolecule or at least polymers for the most part. We need to hydrolyze them.
Why? Why not just keep them together?
65. Know who Linus Pauling was and what he did…
66. Describe the structure of a nucleotide.
67. How many different nucleotides are there that will build RNA and DNA? Be able to
draw them all the way we discussed in class. For example, I could ask you to draw dAMP
or AMP or dCMP etc… or I could ask you to draw an RNA nucleotide containing a
purine base, etc…
68. How do the purines differ structurally from the pyrimidines in general? Know the
names of the bases and to which group they belong, purine or pyrimidine.
69. Draw the ester, diester, phosphoester and phosphdiester functional group.
70. Explain how two nucleotides can be connected together to form a dinucleotide and so
on. You should be able to draw this. What type of linkage is formed? Why do we give it
this name?
71. Compare and contrast a DNA nucleotide to an RNA nucleotide (how are they the
same/different?).
72. What is the overall charge of DNA and why?
73. Describe the ink and the paper analogy for nucleic acid.
74. Describe the backbone of DNA. What do we call the backbone?
75. What is the optimum temperature, pH and salt concentration of proteins? (Explain
why this is a trick question).
76. How would the optimal pH of a protein in the stomach differ from that of a protein in
the blood?
77. Explain what happens to the polymers we are constantly eating. Where did most the
monomers that make up these polymers ultimately come from? (This will require some
logical thinking)
78. How would you define a lipid.
79. How are lipids chemically different from the other three classes of macromolecules?
80. Identify the four types of lipids discussed in class. Be able to draw each type as we
discussed in class.
81. How do unsaturated and saturated fatty acids differ structurally? How does this
change how they behave relative to each other?
82. Where does one find unsaturated fats in nature? What about saturated fats?
83. What does the term “hydrogenated vegetable oil” refer to? Give an example of
hydrogenated vegetable oil that you might find in the supermarket.
84. Describe why saturated fats tend to be solid at room temperature while unsaturated
fats tend to be liquid.
85. Identifty the functions of triglycerides in nature. Where are they found (what type of
cell and what location in humans)? What is the name given to the two general types of fat
in humans? Which is more prevalent in males? Females?
86. Compare and contrast a phospholipid to a triglyceride (What is similar and what is
different?).
87. Describe the function of phospholipids in nature.
88. Why do phospholipids tend to always have one kinked fatty acid (one kinked tail)?
89. Define the term amphiphilic.
90. How does the term amphiphilic relate to phospholipids and why is this an important
property of these molecules?
91. Be able to draw the two structures discussed in class that will result if you take moles
of phospholipids and add them to water. Know the names of the two resulting structures.
Which of these structures would be similar to a cell membrane?
92. Describe the general function of cell membranes in the cell.
93. Describe the structure and function of waxes in nature. Identify at least two places
where you would find them, one in plants and one in animals.
94. Describe the general structure of a steroid and give at least four examples in humans.
95. One example of a steroid is cholesterol. Describe the two functions of cholesterol in
our cells that we spoke about in class, and indicate how the structure of this molecule
determines its function.
96. What is a hormone? Which class of macromolecule and which subclass of a second
class can act as hormones in the body?
97. What is a hormone?
98. We learned that steroids can act as hormones (so now you know that protein amino
acid based molecules and steroids can act as hormones - signaling molecules that allow
cells to “talk” to each other that are not typically located near each other). Indicate the
three types of steroid hormones we discussed in class and describe their function in the
body.
99. Why does one not need to ingest cholesterol to survive?
100. How is cholesterol linked to the steroid hormones?
101. Describe Chargaff’s experiments and why the results were critical in determining
the three-dimensional structure of DNA.
102. Chargaff used a technique known in general terms as paper chromatography.
Describe how to do and the purpose of paper chromatography in general. For example, if
I gave you a solution containing a mixture of various food colorings, how might you
separate them? Explain how paper chromatography works.
103. Describe the three-dimensional structure of DNA.
104. Why is it important that the base pairs of DNA be held together by hydrogen bonds
as opposed to using covalent or ionic bonds?
105. One of the DNA strands is typically called the “complementary” strand. Why do you
think this strand was given this name?
106. What do I mean when I say that the strands of DNA are anti-parallel?
107. Be able to label the ends of the DNA strands (5’ and 3’) and be able to explain why
the ends are given these names.
108. Describe the function of DNA in organisms.
109. Explain the charge of DNA.
110. How does the structure of DNA compare to that of RNA? (Three major differences)
111. Identify the three types of RNA discussed in class and give their function.
112. (This will be a question) Describe how the cell is able to make polypeptides using
the information that is encoded in the DNA. Be able to describe it both using the analogy
and using the actual terms. Be sure to include the following words: DNA, mRNA, tRNA,
rRNA, protein, RNA polymerase (why is this enzyme called RNA polymerase?),
ribosome, polypeptide, amino acids, library, paper, transcribe, translate, nuclear pores,
nuclear membrane, guards. You should be able to make a sketch of this process.
113. The process described above is known as “The Central Dogma” of molecular
biology. What does this mean? (google it)
114. Why is the nucleus NOT NOT NOT (just incase you didn’t see the first NOT) the
control center/brain of the cell?
115. (You will see this question for sure) We discussed the RNA world hypothesis in
class. Describe why it is logical to hypothesize that RNA was used by the most primitive
of cells before the evolution of DNA and protein. What specific evidence do we have that
supports this? (I discussed two major pieces of observational evidence) Why couldn’t
protein have come first or DNA have come first?
116. Why do you think protein and DNA exist today? Why aren’t organisms still solely
based on RNA? Use the term natural selection in your answer to these questions.
117. What is the name given to a stretch of DNA that codes (stores the information for)
for mRNA, tRNA or rRNA?
118. How are tRNA’s and rRNA’s made?
119. Describe the structure of the ribosome. What is it made of?
120. Where do the amino acids that our cells use to build polypeptides come from? Yes, I
know they are in the cytoplasm, but before that…
121. List each macromolecule class and identify where you would find each in a cell. The
next step is going to be to put these molecules together and build a cell. You should have
the basic location and function down before we start getting into details.
122. How many pieces of DNA (“books”) are found in a human nucleus? If you attached
all those pieces end to end, how long would the DNA be? Discuss the size of DNA in
terms of actual size and the MSG analogy. What do we call each piece (“book”)? How
many unique books are there? Explain.
123. Describe the Madison square garden analogy and be sure to include every
macromolecule class (the rope, the straight pin (a phospholipid), the baseball, the head of
a pin (a glucose molecule))
124. Explain why hydrophilic molecules like proteins, amino acids, carbohydrates,
nucleic acids, etc… are NOT able to move through a plasma membrane, while small
hydrophobic molecules can.
(Why do you think large hydrophobic molecules have trouble crossing?)
125. Why do think amino acid based hormones require a cell surface receptor protein to
tell the cell what to do (talk to the cell), while steroid hormones typically have protein
receptors inside the cell, in the cytoplasm?
126. Compare a phospholipid bilayer to a phospholipid monolayer. Which would make
up a cell membrane and why is this a logical choice (why would the other not work as a
cell membrane?).
I am stopping here…so much time, and so little to do!