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Download Transgenic Core Facility Pennington Biomedical Research Center
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Pennington Biomedical Research Center Transgenic Core Facility LOUISIANA STATE UNIVERSITY SYSTEM SOLUTIONS THAT WORK FOR YOUR RESEARCH Our Mission The Transgenic Core produces mice for faculty at Pennington Biomedical Research Center as well as investigators at other institutions. The core utilizes pronuclear microinjection and embryonic stem cell technologies to control gene expression in mice. The mission of the core is to provide a service to PBRC and other investigators to allow for the controlled manipulation of gene expression and to facilitate investigators in understanding gene function. The core strives to provide services at prices that are below those that are commercially available. Our Transgenic Core scientists are here to accommodate all of your gene targeting needs from vector construct to chimeric mice. S E R V I C E S AVA I L A B L E Transgene Preparation Pronuclear Microinjection Our Core accepts investigator-supplied transgenes or will work with the investigator to design, construct and purify the transgene. Approximately 200 C57BL/6J one-cell eggs are injected with the transgene. The anticipated time for delivery is 2 months after initial date of pronuclear microinjection. Gene Targeting Service The "turn-key” service our core provides is a more efficient way to introduce lox P sites in genes for cell specific knockout mice. The core will design and construct the targeting vector, electroporate, and screen ES cells. Inject the targeted ES cells and provide chimeric mice. All mice are produced on the C57BL/6J background, eliminating backcrossing. This unique academic service cost is well below commercial rates. Injection of Embryonic Stem Cells Cryopreservation Targeted embryonic stem cells are injected into C57BL/6J blastocyst. Chimeric mice are delivered at approximately 5 weeks of age. Male and female mice are provided by the user. Fertilized embryos and sperm are collected frozen and stored on site. FOR MORE INFORMATION PLEASE CONTACT: Randall Mynatt, Ph.D. Director of Transgenics 6400 Perkins Rd. Baton Rouge, LA 70808 225-763-3100 [email protected]