Download Fluorescence in situ hybridization Detection of the m2A10

Fluorescence in situ hybridization Detection of the m2A10 Transgene Location in the Mosquito
Vector, Anopheles stephensi.
Amanda Ruiz
Mentor: Anthony A. James
Malaria is the leading cause of death and disease in many developing countries. A transgenic
mosquito has been generated through the insertion of a gene, m2A10, that may prevent
transmission of the protozoan parasite, Plasmodium falciparum. We developed a protocol for
fluorescence in situ hybridization (FISH) to localize the sites of transgene integration in the
mosquito chromosomes. FISH is a cytogenetic technique, using fluorescent probes, to detect and
localize specific DNA sequences. We were able to detect red fluorescing probes marking the
sites of the target and endogenous positive control genes. Using this technique, we hope to better
understand the nature of gene insertions through localization of target gene insertion sites in the
genome of transgenic Anopheles stephensi.