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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
THIRD SEMESTER – NOVEMBER 2014
BT 3823 - PLANT BIOTECHNOLOGY
Date : 01/11/2014
Time : 09:00-12:00
Dept. No.
Max. : 100 Marks
PART – A (20 marks)
ANSWER ALL THE QUESTIONS:
1. Choose the correct answer:
(5 x 1 = 5 marks)
1. Which is not a secondary metabolite?
a) Vanillin
b) Vincristin
c) Vinblastin
2. What is the source of Chlorellin?
a) Bacteria
b) Viruses
c) Algae
d) Sucrose
d) Fungi
3. _________ has the highest protein content.
a) Rice
b) Sunflower
c) Soybean
d) Chickpea
4. CTV stands for_________.
a) citrustristeza virus
c) citrustongo virus
5. Nod factors stimulate ________.
a) growth
c) cell division
b) cinnamon tongo virus
d) chickpeatungra virus
b) morphogenesis
d) hormones
II. State whether the following aretrue or false; if false give reason:
6.
7.
8.
9.
10.
(5 x 1 = 5 marks)
Relative humidity required for plant tissue culture is 60%.
Thionin is a pathogenesis related protein.
ICP stands for intron coding protein.
Plant lectins are toxic by nature.
Nod factors present in rhizobia promotes nodulation.
III. Complete the following:
11. Sodium alginate is obtained from ____________.
12. The scientific name of button mushroom is ____________.
13. Bt genes are mostly used in controlling_________.
14. ___________ is a lectin found in plants especially legumes.
15. The word somaclonal variation was coined by___________.
(5 x 1 = 5 marks)
IV. Answer following each, within 50 words only
16.
17.
18.
19.
20.
(5 x 1 = 5 marks)
What is the use of pectinase in protoplast isolation.
Mention the source of LSD and its use.
What are PR proteins?
Describe the role of phytochromes.
Expand EPSPS.
PART – B
Answer the following, each within 500 words only. Draw diagrams wherever necessary
(5 x 8 = 40 marks)
21. a) Explain the production of secondary metabolite.
OR
b) Give an account on Embryo Endosperm Transplant technique.
22. a) Give an account on bacterial resistance genes.
OR
b) Describe the mode of action of Bt gene.
23. a). Write a note on photomorphogenetic effect.
OR
b) Give a note on biosynthesis and molecular basis of action of auxins.
24. a)Explain any two conventional breeding methods for crop improvement?.
OR
b) Briefly write about somaclonal variations.
25. a) Write notes on the nutritive value and advantages of mushroom.
OR
b) Enumerate the use of VAM as a Biofertilizer.
PART – C
Answer any TWO of the following, each within 1500 words only; draw diagrams wherever
necessary:
(2x 20 = 40 marks)
26. Describe the important applications of Plant Tissue Culture.
27. Discussthe massproduction of carrier based immobilization of Cyanobacterial inoculant.
28. Write about the molecular aspects of seed development and also write about the role of abscissic
acid in seed maturation.
29. Give an account on transgenic plants and their uses.
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