A plAtform for Anti-biofilm AssAys
... pharmacy, for nice work environment. I am especially grateful to Daniela for all the endless discussions over and through the wall between our desks, for eminent travel company and delicious cakes! I would also like to thank everyone else in our research group, Pharmaceutical Sciences Laboratory, fo ...
... pharmacy, for nice work environment. I am especially grateful to Daniela for all the endless discussions over and through the wall between our desks, for eminent travel company and delicious cakes! I would also like to thank everyone else in our research group, Pharmaceutical Sciences Laboratory, fo ...
Molecular and functional analyses of lectins in
... characterized yet and may fill the gaps in our understanding of the mode of action of probiotics. This thesis investigates several putative lectin proteins present in gastrointestinal and vaginal Lactobacillus species, regarding their function for the physiology of the lactobacilli and their interac ...
... characterized yet and may fill the gaps in our understanding of the mode of action of probiotics. This thesis investigates several putative lectin proteins present in gastrointestinal and vaginal Lactobacillus species, regarding their function for the physiology of the lactobacilli and their interac ...
Isolation of Salmonella Serovar-Specific Single Domain
... Salmonella enterica serovar Newport. In this proof of concept study, recombinant and serovarspecific Salmonella spp. flagellin and domain 3 (D3) protein antigens were designed and purified. An immune phage display library was established and VHH single domain antibodies (sdAbs) specific for the D3 r ...
... Salmonella enterica serovar Newport. In this proof of concept study, recombinant and serovarspecific Salmonella spp. flagellin and domain 3 (D3) protein antigens were designed and purified. An immune phage display library was established and VHH single domain antibodies (sdAbs) specific for the D3 r ...
Insect-Derived Cecropins Display Activity against
... members injured in Iraq and Afghanistan (5). The genetic adaptability of A. baumannii allows it to gain resistance to a wide spectrum of commercial antibiotics, and the intrinsic presence of various efflux pumps in A. baumannii also contributes to an insensitivity to many antibiotics (6–8), resultin ...
... members injured in Iraq and Afghanistan (5). The genetic adaptability of A. baumannii allows it to gain resistance to a wide spectrum of commercial antibiotics, and the intrinsic presence of various efflux pumps in A. baumannii also contributes to an insensitivity to many antibiotics (6–8), resultin ...
Loss of outer membrane porins in clonally related clinical isolates of
... periplasmic space between OmpK35 and OmpK36 could be an evolutionary advantage for the expression of one porin over the other in contrasting conditions15. While porins play an important role in nutrient acquisition6,16 the nonspecific nature of these porins also allows the passage of small antibioti ...
... periplasmic space between OmpK35 and OmpK36 could be an evolutionary advantage for the expression of one porin over the other in contrasting conditions15. While porins play an important role in nutrient acquisition6,16 the nonspecific nature of these porins also allows the passage of small antibioti ...
Local Inflammation Induces Complement Crosstalk Which Amplifies the Antimicrobial Response
... Figure 1. Infection-inflammation condition enhanced the bacterial killing effect of complement system by the synergistic action of CRP and L-ficolin. (A) Real-time observation of the bacterial killing effect of serum under normal (pH 7.4, 2.5 mM calcium) or infectioninflammation condition (pH 6.5, 2 ...
... Figure 1. Infection-inflammation condition enhanced the bacterial killing effect of complement system by the synergistic action of CRP and L-ficolin. (A) Real-time observation of the bacterial killing effect of serum under normal (pH 7.4, 2.5 mM calcium) or infectioninflammation condition (pH 6.5, 2 ...
Sulfite oxidation in the purple sulfur bacterium
... belong to the sulfite oxidase proteins, which bind a single molybdopterin without a second nucleotide. SoeA falls into the molybdopterin-binding MopB superfamily. In many characterized members of this family molybdopterin is present in the form of a dinucleotide, with two molybdopterin dinucleotide ...
... belong to the sulfite oxidase proteins, which bind a single molybdopterin without a second nucleotide. SoeA falls into the molybdopterin-binding MopB superfamily. In many characterized members of this family molybdopterin is present in the form of a dinucleotide, with two molybdopterin dinucleotide ...
Antibacterial substances and characteristics of the haemolymph of
... Other antibacterial substances Xylander & Nevermann (1990) reported that no inhibition zones were found in agar with suspended Escherichia coli (Migula, 1895) after application of 4 µl of haemolymph of different chilopod and diplopod species, whereas the growth of another gram-negative bacterium, En ...
... Other antibacterial substances Xylander & Nevermann (1990) reported that no inhibition zones were found in agar with suspended Escherichia coli (Migula, 1895) after application of 4 µl of haemolymph of different chilopod and diplopod species, whereas the growth of another gram-negative bacterium, En ...
Spectroscopic Comparisons of the pH Dependencies of Fe
... xanthine oxidase/cytochrome c assay at pH 7.8 (22) or assayed qualitatively by resolving SOD on nondenaturing PAGE and staining with nitroblue tetrazolium (NBT) and riboflavin (23). Protein concentrations were determined using 280 ) 8660, 9190, and 8950 M-1 cm-1 for Mn-SOD, Fe-sub-(Mn)SOD, and apo- ...
... xanthine oxidase/cytochrome c assay at pH 7.8 (22) or assayed qualitatively by resolving SOD on nondenaturing PAGE and staining with nitroblue tetrazolium (NBT) and riboflavin (23). Protein concentrations were determined using 280 ) 8660, 9190, and 8950 M-1 cm-1 for Mn-SOD, Fe-sub-(Mn)SOD, and apo- ...
Analyzing the molecular mechanism Brucella Shivani Goolab Robyn L. Roth
... by means of a signal peptide to form complex β-barrels that span the OM as outer membrane proteins (Omps) (Koebnik et al., 2000). The characteristic β-barrel of Omp is comprised of amphipathic β-strands (with alternating hydrophobic amino acid residues) ensuring their solubility during secretion thr ...
... by means of a signal peptide to form complex β-barrels that span the OM as outer membrane proteins (Omps) (Koebnik et al., 2000). The characteristic β-barrel of Omp is comprised of amphipathic β-strands (with alternating hydrophobic amino acid residues) ensuring their solubility during secretion thr ...
Inducer expulsion and the occurrence of an HPr(Ser-P)
... bacteria. Using intact cells and membrane vesicles, we show that Entemcoccus faecalis exhibits both inducer exclusion and inducer expulsion, and that the latter phenomenon is dependent on the metabolite-activated ATP-dependent HPr(Ser) kinase that phosphorylates Ser-46 in HPr of the phosphotransfera ...
... bacteria. Using intact cells and membrane vesicles, we show that Entemcoccus faecalis exhibits both inducer exclusion and inducer expulsion, and that the latter phenomenon is dependent on the metabolite-activated ATP-dependent HPr(Ser) kinase that phosphorylates Ser-46 in HPr of the phosphotransfera ...
Haemophilus influenzae and the complement system
... The pathogenesis of many microorganisms relies on their capacity to avoid, resist or neutralize the host defense including the complement system [29, 30]. H. influenzae uses two major mechanisms for complement evasion; I) building physical barriers for the activation and deposition of complement pro ...
... The pathogenesis of many microorganisms relies on their capacity to avoid, resist or neutralize the host defense including the complement system [29, 30]. H. influenzae uses two major mechanisms for complement evasion; I) building physical barriers for the activation and deposition of complement pro ...
Exponential ATP amplification through simultaneous regeneration
... This study first tested the detection limit on ATP using the EAA method. While both of the single-product recycling methods could barely detect ATP at 1.0 pM under our standard assay conditions, their integration into the EAA assay allowed 102 pM ATP to be detected easily, yielding a 100-fold improv ...
... This study first tested the detection limit on ATP using the EAA method. While both of the single-product recycling methods could barely detect ATP at 1.0 pM under our standard assay conditions, their integration into the EAA assay allowed 102 pM ATP to be detected easily, yielding a 100-fold improv ...
Shankar2017 - Edinburgh Research Archive
... (e.g. PhD, MPhil, DClinPsychol) at the University of Edinburgh. Please note the following terms and conditions of use: This work is protected by copyright and other intellectual property rights, which are retained by the thesis author, unless otherwise stated. A copy can be downloaded for personal n ...
... (e.g. PhD, MPhil, DClinPsychol) at the University of Edinburgh. Please note the following terms and conditions of use: This work is protected by copyright and other intellectual property rights, which are retained by the thesis author, unless otherwise stated. A copy can be downloaded for personal n ...
Catalogue Code: BA0046 Pack Size: 100 assays
... reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample. KEY FEATURES Fast and sensitive. Use of 100 µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The proce ...
... reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample. KEY FEATURES Fast and sensitive. Use of 100 µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The proce ...
University of Groningen Autoregulation of lactose transport
... control of enzyme/transporter activity. The two regulatory mechanisms differ in the sense that autoregulation of carbohydrate utilization controls the catabolic activities within a specific metabolic pathway, whereas hierarchical control involves the metabolic pathway of the preferred carbohydrate a ...
... control of enzyme/transporter activity. The two regulatory mechanisms differ in the sense that autoregulation of carbohydrate utilization controls the catabolic activities within a specific metabolic pathway, whereas hierarchical control involves the metabolic pathway of the preferred carbohydrate a ...
Proteomic analysis of the biofilm and biofilm-associated Pseudomonas aeruginosa BRIDGITTA STEYN
... Pseudomonas aeruginosa is one of the most studied biofilm-forming organisms and has emerged as a model organism in the study of surface- and biofilm-induced gene expression. The transition from a planktonic to a biofilm mode of growth results in diverse changes in gene expression, which causes the a ...
... Pseudomonas aeruginosa is one of the most studied biofilm-forming organisms and has emerged as a model organism in the study of surface- and biofilm-induced gene expression. The transition from a planktonic to a biofilm mode of growth results in diverse changes in gene expression, which causes the a ...
Characterization of the RND family of multidrug efflux pumps: in
... metals (Table S1) to be considered as seed sequences for the profile. Provalidator clustered these sequences using BLASTCLUST without rejecting any of them since they were found to be non-redundant (less than 60% identical). The generation of a profile requires a multi-alignment of the seed sequence ...
... metals (Table S1) to be considered as seed sequences for the profile. Provalidator clustered these sequences using BLASTCLUST without rejecting any of them since they were found to be non-redundant (less than 60% identical). The generation of a profile requires a multi-alignment of the seed sequence ...
Thesis - University of Minnesota Duluth
... known to form biofilms on a variety of surfaces such as water pipes and within sewage sludge (Schulze-Robbeke 1993, Schulze-Robbeke 1995). Mycobacterial biofilms have also been found in water treatment plants, domestic water supplies (Falkinham 2001, Schwartz 1998), and dental unit waterlines (Schul ...
... known to form biofilms on a variety of surfaces such as water pipes and within sewage sludge (Schulze-Robbeke 1993, Schulze-Robbeke 1995). Mycobacterial biofilms have also been found in water treatment plants, domestic water supplies (Falkinham 2001, Schwartz 1998), and dental unit waterlines (Schul ...
Structure, function, evolution, and application of
... activities, thus enlarging the diversity of biological catalytic activities. The uptake of vitamins is a critical step not only for organisms that strictly depend on vitamins like humans but also for many bacteria, even if they possess biosynthesis pathways for these molecules. Many vitamins are com ...
... activities, thus enlarging the diversity of biological catalytic activities. The uptake of vitamins is a critical step not only for organisms that strictly depend on vitamins like humans but also for many bacteria, even if they possess biosynthesis pathways for these molecules. Many vitamins are com ...
Phylogenetic Co-Occurrence of ExoR, ExoS, and ChvI, Components
... Sel1 domains and (2) extracellular localization. Both of these sequence characteristics would be necessary for any ortholog to function in a manner similar to to ExoR. For ExoS orthologs, we required predictions of structural elements based on the S. meliloti prototype: a Sensor Domain [Pfam: PF1375 ...
... Sel1 domains and (2) extracellular localization. Both of these sequence characteristics would be necessary for any ortholog to function in a manner similar to to ExoR. For ExoS orthologs, we required predictions of structural elements based on the S. meliloti prototype: a Sensor Domain [Pfam: PF1375 ...
Channel-forming proteins in the cell wall of amino
... cell across this membrane. Water, dissolved gases such as carbon dioxide and oxygen and lipid-soluble molecules simply diffuse across the phospholipid bilayer. Water-soluble ions generally pass through small pores with a diameter less than 0.8 nm in the membrane. All other molecules require either c ...
... cell across this membrane. Water, dissolved gases such as carbon dioxide and oxygen and lipid-soluble molecules simply diffuse across the phospholipid bilayer. Water-soluble ions generally pass through small pores with a diameter less than 0.8 nm in the membrane. All other molecules require either c ...
E self-assembled monolayers Jen-Jr Gau , Esther H. Lan
... solution; (7) 10 ml of K-blue substrate (Neogen Corp., 300176) was placed on the detector array in such a way that all three electrodes (working, auxiliary, reference) were covered by the substrate solution; and (8) electrochemical measurements were immediately taken. The entire protocol was complet ...
... solution; (7) 10 ml of K-blue substrate (Neogen Corp., 300176) was placed on the detector array in such a way that all three electrodes (working, auxiliary, reference) were covered by the substrate solution; and (8) electrochemical measurements were immediately taken. The entire protocol was complet ...
Output Interpretation - UCSF Viral Diagnostics and Discovery Center
... Reads that are not aligned to NCBI nt DB are mapped by translated nucleotide alignment against a viral protein database at lowstringency using RAPSearch. This process identifies divergent viral reads. However, due to the low-stringency parameters required to detect divergent viruses (default e-value ...
... Reads that are not aligned to NCBI nt DB are mapped by translated nucleotide alignment against a viral protein database at lowstringency using RAPSearch. This process identifies divergent viral reads. However, due to the low-stringency parameters required to detect divergent viruses (default e-value ...
Environmental factors influence virulence of Pseudomonas
... Figure (22) Pyochelin production by P. aeruginosa PA14 wild-type control and the phoB mutant under low and high phosphate conditions. .......................................... 79 Figure (23) Pyoverdine production by P. aeruginosa PA14 wild-type control and the phoB mutant under low and high phospha ...
... Figure (22) Pyochelin production by P. aeruginosa PA14 wild-type control and the phoB mutant under low and high phosphate conditions. .......................................... 79 Figure (23) Pyoverdine production by P. aeruginosa PA14 wild-type control and the phoB mutant under low and high phospha ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.