A microwave and detergent procedure to detect high
... by other components of the lysed cell; they are detected with less sensitivity by most commercial ion detectors. This poster reports a procedure using acid-cleavable detergent and microwave to facilitate desorption of higher molecular weight protein biomarkers from lysed whole cells. This approach h ...
... by other components of the lysed cell; they are detected with less sensitivity by most commercial ion detectors. This poster reports a procedure using acid-cleavable detergent and microwave to facilitate desorption of higher molecular weight protein biomarkers from lysed whole cells. This approach h ...
Gemeinsames Kolloquium
... compartments is organized. How does a cyanobacterial cell "decide" which proteins are translocated into the thylakoid lumen and which proteins are sorted into the periplasmic space? This "sorting problem" is essentially completely unresolved yet. I will present and discuss our recent work on protein ...
... compartments is organized. How does a cyanobacterial cell "decide" which proteins are translocated into the thylakoid lumen and which proteins are sorted into the periplasmic space? This "sorting problem" is essentially completely unresolved yet. I will present and discuss our recent work on protein ...
Localization of Leptospiral surface proteins by biotinylation
... for subsurface, preferably periplasmic proteins) to assess the integrity of the outer membrane during biotinylation labeling of intact cells. Method: 1. Grow Leptospira in EMJH medium, supplemented with 1% rabbit serum at 30° C until they reach density of ~5 x 108 cells/ml. 2. Harvest Leptospira ...
... for subsurface, preferably periplasmic proteins) to assess the integrity of the outer membrane during biotinylation labeling of intact cells. Method: 1. Grow Leptospira in EMJH medium, supplemented with 1% rabbit serum at 30° C until they reach density of ~5 x 108 cells/ml. 2. Harvest Leptospira ...
Nutraferma® Launches NF8™ Protein Product
... A value-added functional soy-protein for young animal diets N. SIOUX CITY, SD – May 28, 2014 – Nutraferma, a leading innovator of biotech solutions, has launched NF8™, a state-of-the-art functional soy-protein ingredient for young animal diets. NF8 contains live, patented viable microorganisms such ...
... A value-added functional soy-protein for young animal diets N. SIOUX CITY, SD – May 28, 2014 – Nutraferma, a leading innovator of biotech solutions, has launched NF8™, a state-of-the-art functional soy-protein ingredient for young animal diets. NF8 contains live, patented viable microorganisms such ...
SOS/umu-Test as A Tool for Genotoxicity Assessment
... • ONPG (colorless) will be converted into onitrophenyl (yellow color) by the existance of β-galactosidase ...
... • ONPG (colorless) will be converted into onitrophenyl (yellow color) by the existance of β-galactosidase ...
Scorpion Sodium Toxin Expression Studies for Proteomics
... genomic analyses. One of the new methods, Ligation Independent Cloning (LIC), is considered a robust alternative to standard methods to generate a wide variety of proteins from isolated genes. With the LIC technique, many different protein subregions (domains and domain combinations) can be created ...
... genomic analyses. One of the new methods, Ligation Independent Cloning (LIC), is considered a robust alternative to standard methods to generate a wide variety of proteins from isolated genes. With the LIC technique, many different protein subregions (domains and domain combinations) can be created ...
The Role of Protein in Dairy Cattle Nutrition
... microbial protein. The blend of amino acids available to the cow is a combination of those released by intestinal digestion of both microbial and undegraded feed proteins. The total amount of protein available to the animal for metabolism is referred to as Metabolizable Protein. When the blend of am ...
... microbial protein. The blend of amino acids available to the cow is a combination of those released by intestinal digestion of both microbial and undegraded feed proteins. The total amount of protein available to the animal for metabolism is referred to as Metabolizable Protein. When the blend of am ...
C19459 C-Reactive Protein V3.indd
... Acute inflammation can cause an increase in the concentration of circulating CRP of up to 5,000-fold that peaks 48 hours after the initial inflammatory stimulus. The blood concentration is indicative of the severity of the stimulus. CRP binds to phosphorylcholine on the surface of bacteria and is th ...
... Acute inflammation can cause an increase in the concentration of circulating CRP of up to 5,000-fold that peaks 48 hours after the initial inflammatory stimulus. The blood concentration is indicative of the severity of the stimulus. CRP binds to phosphorylcholine on the surface of bacteria and is th ...
TolC-like Burkholderia
... mechanisms of pathogenesis is crucial to identify new drug targets against these microorganisms. Several species of Burkholderia have the genome fully sequenced and have genetic information for the synthesis of several extracellular proteins like hemolysins, lipases and proteases that are virulence ...
... mechanisms of pathogenesis is crucial to identify new drug targets against these microorganisms. Several species of Burkholderia have the genome fully sequenced and have genetic information for the synthesis of several extracellular proteins like hemolysins, lipases and proteases that are virulence ...
Document
... • Insluin: 51 amino acids, and has a molecular weight of 5808 Da. It is a dimer of an A-chain and a B-chain, which are linked together by disulfide bonds. • Serious problem for Diabetes mellitus ...
... • Insluin: 51 amino acids, and has a molecular weight of 5808 Da. It is a dimer of an A-chain and a B-chain, which are linked together by disulfide bonds. • Serious problem for Diabetes mellitus ...
Picobiology
... box. In order to unveil the black box, vibrational spectroscopic analysis is required as well. It provides us with structure at a resolution of 1 pm (0.01Å) sufficient to detect the difference of chemical reactivity of functional groups. If a bond length is elongated by only 1 pm from an equilibrium ...
... box. In order to unveil the black box, vibrational spectroscopic analysis is required as well. It provides us with structure at a resolution of 1 pm (0.01Å) sufficient to detect the difference of chemical reactivity of functional groups. If a bond length is elongated by only 1 pm from an equilibrium ...
Biochemical composition of blood in norm and pathology acute
... Albumin constitutes about 60% of the total protein in plasma and is present at concentrations between 35 and 55 mg/mL. It is the main contributor to osmotic pressure of the blood and it functions as a carrier molecule for molecules with low water solubility such as lipid soluble hormones, enzymes, f ...
... Albumin constitutes about 60% of the total protein in plasma and is present at concentrations between 35 and 55 mg/mL. It is the main contributor to osmotic pressure of the blood and it functions as a carrier molecule for molecules with low water solubility such as lipid soluble hormones, enzymes, f ...
Title: Metal sensor domains of signal transdution signals
... The first stage of this project is to isolate the genes that compose the E.coli Fe2+ operon using PCR. These genes will be inserted into expression plasmids for protein over-expression. The expression conditions will be optimized for rich and minimum (defined) media that is suitable for isotopic lab ...
... The first stage of this project is to isolate the genes that compose the E.coli Fe2+ operon using PCR. These genes will be inserted into expression plasmids for protein over-expression. The expression conditions will be optimized for rich and minimum (defined) media that is suitable for isotopic lab ...
Document
... (UBA, UIM, UBZ). Some binding partners require a particular chain, others are substrate-specific. ...
... (UBA, UIM, UBZ). Some binding partners require a particular chain, others are substrate-specific. ...
How proteins synthesized in the cytoplasm of pathogenic bacteria
... translocation process is carried out through the Type 3 Secretion System (T3SS), a highly conserved secretion system composed by more than twenty different proteins. The T3SS resembles a nano-syringe able to engage the host plasma membrane and allow: 1) The secretion of toxic proteins through both b ...
... translocation process is carried out through the Type 3 Secretion System (T3SS), a highly conserved secretion system composed by more than twenty different proteins. The T3SS resembles a nano-syringe able to engage the host plasma membrane and allow: 1) The secretion of toxic proteins through both b ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.