SUPPLEMENTAL MATERIALS Deciphering protein dynamics of the
... suicide vectors from E. coli S17-1 to the PAO1 strains and integration of the plasmids into the chromosome, with selection for tetracycline resistance. A second crossing-over event excising the vector was achieved by enrichment for tetracycline-sensitive cells [2] to generate the corresponding muta ...
... suicide vectors from E. coli S17-1 to the PAO1 strains and integration of the plasmids into the chromosome, with selection for tetracycline resistance. A second crossing-over event excising the vector was achieved by enrichment for tetracycline-sensitive cells [2] to generate the corresponding muta ...
Close - Journal of Integrated OMICS
... bacteria such as E. coli in wild animals could show whether wildlife serves as an environmental reservoir or “melting pot” of bacterial resistance. Proteomics techniques can be used to quantitatively compare the expression of protein in different conditions to obtain a better understanding of the ce ...
... bacteria such as E. coli in wild animals could show whether wildlife serves as an environmental reservoir or “melting pot” of bacterial resistance. Proteomics techniques can be used to quantitatively compare the expression of protein in different conditions to obtain a better understanding of the ce ...
Natural Product Reports
... The CusCFBA system in E. coli is a CBA-type transporter with an additional fourth component. The Cus system consists of CusA, the inner membrane RND transporter, CusC, the outer membrane factor, CusB, the periplasmic adaptor protein, and CusF, the small periplasmic protein, all of which are encoded ...
... The CusCFBA system in E. coli is a CBA-type transporter with an additional fourth component. The Cus system consists of CusA, the inner membrane RND transporter, CusC, the outer membrane factor, CusB, the periplasmic adaptor protein, and CusF, the small periplasmic protein, all of which are encoded ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
... . The different band was protein 15.13 kDa (figure 3). This band appeared only in E. gergoviae 1. A study of Staphylococcus aureus showed the result of SDS-PAGE from positive biofilm bacteria in an enrichment medium and in nutrient broth had a lower molecular weight protein31. Contrary to Loehfelm e ...
... . The different band was protein 15.13 kDa (figure 3). This band appeared only in E. gergoviae 1. A study of Staphylococcus aureus showed the result of SDS-PAGE from positive biofilm bacteria in an enrichment medium and in nutrient broth had a lower molecular weight protein31. Contrary to Loehfelm e ...
transport of molecules across microbial membranes
... is because it is so dicult to analyse the structures of the membrane proteins that mediate, or play central roles in, these processes. Membrane proteins are inherently dicult to purify and crystallize in (active) forms suitable for highresolution analysis, because they are amphipathic molecules. T ...
... is because it is so dicult to analyse the structures of the membrane proteins that mediate, or play central roles in, these processes. Membrane proteins are inherently dicult to purify and crystallize in (active) forms suitable for highresolution analysis, because they are amphipathic molecules. T ...
Foundations in Microbiology
... potential agents of infection and spoilage without destroying the food flavor or value 63°C–66°C for 30 minutes (batch method) 71.6°C for 15 seconds (flash method) Not sterilization – kills non-sporeforming pathogens and lowers overall microbe count; does not kill endospores or many nonpathogenic mi ...
... potential agents of infection and spoilage without destroying the food flavor or value 63°C–66°C for 30 minutes (batch method) 71.6°C for 15 seconds (flash method) Not sterilization – kills non-sporeforming pathogens and lowers overall microbe count; does not kill endospores or many nonpathogenic mi ...
Virtual Screening of Potential Drug-like Inhibitors against MexB Efflux Protein
... Hydrogen bonding measures the intermolecular interaction between the protein and ligands. The compound possessed 1.78 score in the hydrophobic map prediction, hppmap tool of Schrodinger suite (Fig. 5) proved its strong binding and interaction. The shortlisted inhibitor docked with better Glide score ...
... Hydrogen bonding measures the intermolecular interaction between the protein and ligands. The compound possessed 1.78 score in the hydrophobic map prediction, hppmap tool of Schrodinger suite (Fig. 5) proved its strong binding and interaction. The shortlisted inhibitor docked with better Glide score ...
Exam for 5MO019: Gene regulation and expression technology
... regulatory protein bound upstream. What are they? Give a short explanation of how these solutions allow the RNA polymerase to interact with a regulatory protein bound at a distance from the RNA polymerase binding site. Intrinsic DNA bends (caused by polyA or polyT tracts) are found in the sequence g ...
... regulatory protein bound upstream. What are they? Give a short explanation of how these solutions allow the RNA polymerase to interact with a regulatory protein bound at a distance from the RNA polymerase binding site. Intrinsic DNA bends (caused by polyA or polyT tracts) are found in the sequence g ...
11_literature rwview
... Fusobacteria are obligately anaerobic non-sporeforming gram-negative bacilli. Since the first reports in the late nineteenth century, various names have been applied to these organisms, sometimes with the same name being applied to different species. More recently, not only have there been changes t ...
... Fusobacteria are obligately anaerobic non-sporeforming gram-negative bacilli. Since the first reports in the late nineteenth century, various names have been applied to these organisms, sometimes with the same name being applied to different species. More recently, not only have there been changes t ...
chapt11_lecture_edit
... Water fluoridation - controlled addition of fluoride to a public water supply to reduce tooth decay Fluoride ions remain in the saliva/on teeth and control plaque preventing development of tooth-decaying microbes Improper implementation of water fluoridation can result in acute fluoride poisoning; s ...
... Water fluoridation - controlled addition of fluoride to a public water supply to reduce tooth decay Fluoride ions remain in the saliva/on teeth and control plaque preventing development of tooth-decaying microbes Improper implementation of water fluoridation can result in acute fluoride poisoning; s ...
Pseudomonas aeruginosa S
... with the molecular weight of 426.46, H-bond donor of 2, H-bond acceptor of 9.45 and logPo/w of 2.66. ...
... with the molecular weight of 426.46, H-bond donor of 2, H-bond acceptor of 9.45 and logPo/w of 2.66. ...
Exam 1 Key - chem.uwec.edu
... 4. Why do many drugs containing ester functional groups have a limited duration of action? a. The ester groups make the drug more polar and reduce absorption. *b. The ester groups are susceptible to esterase enzymes. c. The ester groups act as steric shields and prevent drugs entering binding sites. ...
... 4. Why do many drugs containing ester functional groups have a limited duration of action? a. The ester groups make the drug more polar and reduce absorption. *b. The ester groups are susceptible to esterase enzymes. c. The ester groups act as steric shields and prevent drugs entering binding sites. ...
Myriam Hönig
... against the dreaded hospital pathogen Staphylococcus aureus: A highly effective protein from bacteria-specific viruses, so-called bacteriophages, shall rapidly kill the bacteria, which frequently occur in the nose. Due to the specific action, the natural microflora is maintained. Such prophylactic t ...
... against the dreaded hospital pathogen Staphylococcus aureus: A highly effective protein from bacteria-specific viruses, so-called bacteriophages, shall rapidly kill the bacteria, which frequently occur in the nose. Due to the specific action, the natural microflora is maintained. Such prophylactic t ...
Proteomic analysis of Escherichia coli with experimentally
... The development and selection of resistant bacteria are affected by several factors, including the biological fitness costs and the ability to compensate for such costs. Antibiotic resistance often confers a metabolic cost because the resistance mutations typically occur in genes of target molecules ...
... The development and selection of resistant bacteria are affected by several factors, including the biological fitness costs and the ability to compensate for such costs. Antibiotic resistance often confers a metabolic cost because the resistance mutations typically occur in genes of target molecules ...
X-Verter - iGEM 2006
... oscillator and Predator Prey a two stage two population oscillator. Created an open source gene circuit IDE called Biobrick Manager. Creation and characterization in a mammalian system of a DNA and light “alphabet” for Human Encryption. Future uses include national security and health detection appl ...
... oscillator and Predator Prey a two stage two population oscillator. Created an open source gene circuit IDE called Biobrick Manager. Creation and characterization in a mammalian system of a DNA and light “alphabet” for Human Encryption. Future uses include national security and health detection appl ...
A dps-Like Gene with the Host Bacterium Revealed by Comparative
... DNA in vitro and was proposed to protect DNA from oxidative damage [1]. Its name DNA-binding protein from starved cell (Dps) has been widely accepted. However, some proteins belonging to the same family, which was isolated earlier, do not bind DNA, that is, the 4D antigen of Treponema pallidum isola ...
... DNA in vitro and was proposed to protect DNA from oxidative damage [1]. Its name DNA-binding protein from starved cell (Dps) has been widely accepted. However, some proteins belonging to the same family, which was isolated earlier, do not bind DNA, that is, the 4D antigen of Treponema pallidum isola ...
Applied and Environmental Microbiology
... (anodic side 2). Additional protein binding sites on the membrane were saturated by soaking the membrane in 3% (wt/vol) bovine serum albumin in phosphate-buffered saline (PBS) buffer for 20 min at room temperature, and the membranes were washed with PBS containing 0.05% Tween 20. The membranes were ...
... (anodic side 2). Additional protein binding sites on the membrane were saturated by soaking the membrane in 3% (wt/vol) bovine serum albumin in phosphate-buffered saline (PBS) buffer for 20 min at room temperature, and the membranes were washed with PBS containing 0.05% Tween 20. The membranes were ...
5th International Conference on Biomolecular
... Some format issues inherent in the e-media version may also appear in this print version. ...
... Some format issues inherent in the e-media version may also appear in this print version. ...
El Proyecto Genoma Humano
... Entrez Protein Clusters http://www.ncbi.nlm.nih.gov/sites/entrez?db=proteinclusters ...
... Entrez Protein Clusters http://www.ncbi.nlm.nih.gov/sites/entrez?db=proteinclusters ...
The Bacterial Chromosome: Structure and Function
... into biochemically and structurally related fibers 4-12 nm wide Amyloidogenic proteins are mostly unstructured or contain mixtures of β-sheets and α-helices in their native structure ...
... into biochemically and structurally related fibers 4-12 nm wide Amyloidogenic proteins are mostly unstructured or contain mixtures of β-sheets and α-helices in their native structure ...
SYLVIA ELLINGER, RUDI GLOCKSHUBER, GERHARD JAHN, 1
... HIV proteins or protein fragments in bacteria has turned out to be a particularly effective alternative (4, 9, 12). It is safe and inexpensive, and the yields of viral proteins are high. Additionally, genetic manipulations (e.g., the construction of truncated mutants or strain variants) can be perfo ...
... HIV proteins or protein fragments in bacteria has turned out to be a particularly effective alternative (4, 9, 12). It is safe and inexpensive, and the yields of viral proteins are high. Additionally, genetic manipulations (e.g., the construction of truncated mutants or strain variants) can be perfo ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.