identification and analysis of proteins and genes responsible for
... strict anaerobic and denaturing conditions and a highly purified form of the enzyme was obtained. Possible solutions to avoid the formation of inclusion bodies while still maintaining the enzyme activity were discussed. This work proved the feasibility of recombinant perchlorate reductase expression ...
... strict anaerobic and denaturing conditions and a highly purified form of the enzyme was obtained. Possible solutions to avoid the formation of inclusion bodies while still maintaining the enzyme activity were discussed. This work proved the feasibility of recombinant perchlorate reductase expression ...
Alignment and Structure Prediction of Divergent Protein Families
... been discovered in prokaryotes, coiled coils are believed to be present in some periplasmic protein domains (Engel et al., 1992; McLachlan, 1978; Scott et al., 1993). Thus, the occurrence of coiled coils in the PEP family (previously predicted for several PEPs; Pimenta et al., 1996) would not be sur ...
... been discovered in prokaryotes, coiled coils are believed to be present in some periplasmic protein domains (Engel et al., 1992; McLachlan, 1978; Scott et al., 1993). Thus, the occurrence of coiled coils in the PEP family (previously predicted for several PEPs; Pimenta et al., 1996) would not be sur ...
Characterization of Bartonella-Bacilliformis Flagella and Effect of
... Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human ...
... Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human ...
The MarR Family of Transcriptional Regulators – A
... Similar to MarR, MexR negatively regulates an operon in Pseudomonas aeruginosa that, when expressed, encodes a tri-partite multidrug efflux system that results in increased resistance to multiple antibiotics, including tetracycline, -lactams, chloramphenicol, novobiocin, sulfonamides and fluoroquin ...
... Similar to MarR, MexR negatively regulates an operon in Pseudomonas aeruginosa that, when expressed, encodes a tri-partite multidrug efflux system that results in increased resistance to multiple antibiotics, including tetracycline, -lactams, chloramphenicol, novobiocin, sulfonamides and fluoroquin ...
Detection of Antibodies Against Zoonotic Food Borne Pathogens in
... conjugate dilutions were chosen after preliminary checker board titration. In the present study, the optimum conditions were 40 µg mL 1 coating buffer antigen concentration, 1:100 serum dilutions, and 1:1000 Horse radish peroxidase- labeled anti-human IgG (Sigma Co.) as conjugate and 1 mg p-nitrophe ...
... conjugate dilutions were chosen after preliminary checker board titration. In the present study, the optimum conditions were 40 µg mL 1 coating buffer antigen concentration, 1:100 serum dilutions, and 1:1000 Horse radish peroxidase- labeled anti-human IgG (Sigma Co.) as conjugate and 1 mg p-nitrophe ...
Protein synthesis inhibitors: Chloramphenicol, Tetracyclines
... o Eye infection** .. Topical o Melioidosis … Burkholderia pseudomalleri (gram negative rods) ...
... o Eye infection** .. Topical o Melioidosis … Burkholderia pseudomalleri (gram negative rods) ...
CURRENT STATUS OF RICKETTSIA DISEASES RESEARCH AT …
... (range 0.2μM – 0.7μM), MgCl2 (ranges 3mM – 7mM and 3.5mM – 7.5mM), and annealing temperature (range 56˚C - 66˚C). For each test, 101 copies/μl and 102 copies/μl of DNA templates were used. Optimal conditions were determined based on the lowest cycle threshold values of logarithmic fluorescence using ...
... (range 0.2μM – 0.7μM), MgCl2 (ranges 3mM – 7mM and 3.5mM – 7.5mM), and annealing temperature (range 56˚C - 66˚C). For each test, 101 copies/μl and 102 copies/μl of DNA templates were used. Optimal conditions were determined based on the lowest cycle threshold values of logarithmic fluorescence using ...
Interacting networks of resistance, virulence and core
... broad range of beta lactam antibiotics, alterations of all three PBPs are necessary. Consequently, the emergence of multidrug-resistant pneumococci is associated with transformation events that alter all three over very short evolutionary timescales (29-31). Another factor that might underlie both t ...
... broad range of beta lactam antibiotics, alterations of all three PBPs are necessary. Consequently, the emergence of multidrug-resistant pneumococci is associated with transformation events that alter all three over very short evolutionary timescales (29-31). Another factor that might underlie both t ...
in Peptide Synthesis, Molecular Recognition
... living systems by designing synthetic models that mimic essential features of biomolecules. In view of the expected exponential growth of knowledge within the next decade about structure-activity relationships in bioactive compounds as well as about mechanisms of molecular recognition in cellular co ...
... living systems by designing synthetic models that mimic essential features of biomolecules. In view of the expected exponential growth of knowledge within the next decade about structure-activity relationships in bioactive compounds as well as about mechanisms of molecular recognition in cellular co ...
5Poster012407 - Thomas Jefferson High School for Science and
... (range 0.2μM – 0.7μM), MgCl2 (ranges 3mM – 7mM and 3.5mM – 7.5mM), and annealing temperature (range 56˚C - 66˚C). For each test, 101 copies/μl and 102 copies/μl of DNA templates were used. Optimal conditions were determined based on the lowest cycle threshold values of logarithmic fluorescence using ...
... (range 0.2μM – 0.7μM), MgCl2 (ranges 3mM – 7mM and 3.5mM – 7.5mM), and annealing temperature (range 56˚C - 66˚C). For each test, 101 copies/μl and 102 copies/μl of DNA templates were used. Optimal conditions were determined based on the lowest cycle threshold values of logarithmic fluorescence using ...
Molecular studies on streptococcal surface proteins Waldemarsson
... surface proteins expressed by S. agalactiae and S. pyogenes and, more specifically, the molecular and biological characterization of these proteins. The first paper describes the novel Blr protein of S. agalactiae, which together with the previously described Slr protein of S. pyogenes identifies a ...
... surface proteins expressed by S. agalactiae and S. pyogenes and, more specifically, the molecular and biological characterization of these proteins. The first paper describes the novel Blr protein of S. agalactiae, which together with the previously described Slr protein of S. pyogenes identifies a ...
EmrE, THE SMALLEST ION-COUPLED TRANSPORTER
... residues are otherwise unavailable to any of the other reagents tested, whether hydrophobic, hydrophilic, positively or negatively charged and even though they are smaller and at least as reactive as the mercurials. Because PCMB shows structural similarity to other substrates of EmrE and because it ...
... residues are otherwise unavailable to any of the other reagents tested, whether hydrophobic, hydrophilic, positively or negatively charged and even though they are smaller and at least as reactive as the mercurials. Because PCMB shows structural similarity to other substrates of EmrE and because it ...
The Rare Outer Membrane Protein, OmpL1, of Pathogenic
... with amplification for 30 cycles under standard conditions, using the above synthetic oligonucleotides and Thermus aquaticus polymerase (Promega). L. kirschneri genomic DNA, prepared as described previously (56), was used as template. The amplified ompL1 gene was then ligated into pBluescript KS(1) ...
... with amplification for 30 cycles under standard conditions, using the above synthetic oligonucleotides and Thermus aquaticus polymerase (Promega). L. kirschneri genomic DNA, prepared as described previously (56), was used as template. The amplified ompL1 gene was then ligated into pBluescript KS(1) ...
bacteriology1 review 2016 AY
... nosocomial) and toxinoses (food poisoning, exfoliative skin disease, Toxic shock) ...
... nosocomial) and toxinoses (food poisoning, exfoliative skin disease, Toxic shock) ...
In silico identification of novel candidate drug targets in
... have been correlated with significantly higher levels of IgA1 protease activity as compared to strains containing only the igagene. Lipooligosaccharides: Non-Typeable H. influenzae attacks the host cell by binding to PAF (platelet-activating factor) receptor via their LOS glycoforms that contains ph ...
... have been correlated with significantly higher levels of IgA1 protease activity as compared to strains containing only the igagene. Lipooligosaccharides: Non-Typeable H. influenzae attacks the host cell by binding to PAF (platelet-activating factor) receptor via their LOS glycoforms that contains ph ...
Mechanisms of bacterial resistance to antibiotics - Micro-Rao
... Mutational narrowing of porin channels 4. Active efflux Mutational overexpression of efflux pumps Plasmid mediated novel efflux pumps Mutations making efflux-pump more efficient at export 5. Antibiotic sequestration: Multilayered thick cell wall binds to antibiotics (glycopeptide resistance ...
... Mutational narrowing of porin channels 4. Active efflux Mutational overexpression of efflux pumps Plasmid mediated novel efflux pumps Mutations making efflux-pump more efficient at export 5. Antibiotic sequestration: Multilayered thick cell wall binds to antibiotics (glycopeptide resistance ...
AMERICAN SOCIETY FOR MICROBIOLOGY VIRGINIA BRANCH
... membranes, and due to the turnover of enterocytes, EA is present in the GI tract at concentrations that support growth of EHEC. EA is not only an important metabolite for EHEC but also serves as a signal of the host environment. We have determined that EA influences EHEC adherence to epithelial and ...
... membranes, and due to the turnover of enterocytes, EA is present in the GI tract at concentrations that support growth of EHEC. EA is not only an important metabolite for EHEC but also serves as a signal of the host environment. We have determined that EA influences EHEC adherence to epithelial and ...
The emerging physiological roles of the glycerophosphodiesterase
... spirochete has been detected upon infection [41,42]. Moreover, the cloning of Gpd homologs in several Borreliae species, and their conservation, has allowed the setting-up of molecular and serologic techniques for the diagnosis of relapsing fever borreliosis [43–45]. Intriguingly, the search for the ...
... spirochete has been detected upon infection [41,42]. Moreover, the cloning of Gpd homologs in several Borreliae species, and their conservation, has allowed the setting-up of molecular and serologic techniques for the diagnosis of relapsing fever borreliosis [43–45]. Intriguingly, the search for the ...
Complete genome determination and analysis of Acholeplasmataceae Acholeplasma oculi
... resulting in abnormal growth and reduced vitality. No general evidence for pathogenesis by acholeplasmas in colonised insects and plants has been provided to date. However, a recently published study on the A. laidlawii strain PG-8 supports its phytopathogenicity, which can be increased after nanotr ...
... resulting in abnormal growth and reduced vitality. No general evidence for pathogenesis by acholeplasmas in colonised insects and plants has been provided to date. However, a recently published study on the A. laidlawii strain PG-8 supports its phytopathogenicity, which can be increased after nanotr ...
The Staphylococci - IRSC Biology Department
... SLIDE AGGLUTINATION • Latex beads are coated antibodies for Protein A. – Protein A is a surface protein found on Staphylococcus aureus ...
... SLIDE AGGLUTINATION • Latex beads are coated antibodies for Protein A. – Protein A is a surface protein found on Staphylococcus aureus ...
Type III Protein Secretion in Plant Pathogenic Bacteria
... may make the N-terminal regions of T3S substrate proteins structurally flexible and probably unfolded, an important prerequisite for their transport through the narrow inner channel of the T3SS, which is presumably only 2.8 nm in diameter as was shown for the T3SS from animal pathogenic bacteria (Ma ...
... may make the N-terminal regions of T3S substrate proteins structurally flexible and probably unfolded, an important prerequisite for their transport through the narrow inner channel of the T3SS, which is presumably only 2.8 nm in diameter as was shown for the T3SS from animal pathogenic bacteria (Ma ...
Protease - Acsu Buffalo
... One step purification that yields a highly enriched sample ready for biochemical and some structural studies (NMR and CD) Hexahis (6xHis) : immobilized nickle, cobalt Affinity tags (e.g. hemagglutinin tag) : affinity column made of monoclonal antibody Biotinin : streptavidin, avidin ...
... One step purification that yields a highly enriched sample ready for biochemical and some structural studies (NMR and CD) Hexahis (6xHis) : immobilized nickle, cobalt Affinity tags (e.g. hemagglutinin tag) : affinity column made of monoclonal antibody Biotinin : streptavidin, avidin ...
to Celtone/Spectra 9 information
... and provides excellent cell growth and high protein expression. ...
... and provides excellent cell growth and high protein expression. ...
Document
... The LECTIN pathway Lectins are proteins which bind to carbohydrates. Many bacteria have many mannose residues on their surface. The lectin-based complement system begins with a “mannose-binding protein” (MBP). MBP reacts, in turn, with a MBP-associated serine protease (MASP). MASP functions, in eff ...
... The LECTIN pathway Lectins are proteins which bind to carbohydrates. Many bacteria have many mannose residues on their surface. The lectin-based complement system begins with a “mannose-binding protein” (MBP). MBP reacts, in turn, with a MBP-associated serine protease (MASP). MASP functions, in eff ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.