of two
... one gene copy. The domains architecture of bacterial CCPs is simpler, consisting in the amino-terminal (N-domain) and the carboxypeptidase domain (CP domain) both conserved in the subfamily. In humans there are six paralogous genes (CCP1-CCP6) and, besides the Nand CP-domains, they contain additiona ...
... one gene copy. The domains architecture of bacterial CCPs is simpler, consisting in the amino-terminal (N-domain) and the carboxypeptidase domain (CP domain) both conserved in the subfamily. In humans there are six paralogous genes (CCP1-CCP6) and, besides the Nand CP-domains, they contain additiona ...
BpaB, a novel protein encoded by the Lyme disease spirochete`s
... 5444 Nucleic Acids Research, 2010, Vol. 38, No. 16 biochemical studies revealed that two small regions 50 of erp loci contain binding sites for B. burgdorferi cytoplasmic proteins (20). The DNA region closest to the transcriptional start site, Operator 2, appears to play a role in transcriptional re ...
... 5444 Nucleic Acids Research, 2010, Vol. 38, No. 16 biochemical studies revealed that two small regions 50 of erp loci contain binding sites for B. burgdorferi cytoplasmic proteins (20). The DNA region closest to the transcriptional start site, Operator 2, appears to play a role in transcriptional re ...
The Staphylococci123.5 KB
... Produce green pigment on blood agar media Require complex media supplemented with blood products and inc. @ 5-10 % CO2 atmsp. ...
... Produce green pigment on blood agar media Require complex media supplemented with blood products and inc. @ 5-10 % CO2 atmsp. ...
Datasheet PDF - BioAssay Systems
... refer to Material Safety Data Sheet for detailed information. ...
... refer to Material Safety Data Sheet for detailed information. ...
Phenotype
... 33. Bateman A, Coin L, Durbin R, Finn RD, Hollich V, et al. UDP-3-O-acyl N-acetylglycosamine deacetylase: "The enzymes in this family catalyse the second step in the biosynthetic pathway for lipid A. " (PF03331) The Pfam Protein Families Database. http://www.sanger.ac.uk//cgi-bin/Pfam/getacc?PF03331 ...
... 33. Bateman A, Coin L, Durbin R, Finn RD, Hollich V, et al. UDP-3-O-acyl N-acetylglycosamine deacetylase: "The enzymes in this family catalyse the second step in the biosynthetic pathway for lipid A. " (PF03331) The Pfam Protein Families Database. http://www.sanger.ac.uk//cgi-bin/Pfam/getacc?PF03331 ...
Import of bacterial pathogenicity factors into mitochondria
... proteins may be identified in phenotypic screens. For example, a connection was established between chlamydial infection and mitochondria in a recent cytotoxicity screen in yeast. One of the proteins significantly impairing yeast growth was shown to target mitochondria [38]. So far, nearly all bacte ...
... proteins may be identified in phenotypic screens. For example, a connection was established between chlamydial infection and mitochondria in a recent cytotoxicity screen in yeast. One of the proteins significantly impairing yeast growth was shown to target mitochondria [38]. So far, nearly all bacte ...
Development of novel and high value products in green algae
... To get 1kg of osteopontin from algae a company would only need to produce 100kg of biomass • Algae biotechnology opens up new opportunities in health and nutrition • Advances made by algae cultivators can be applied to algal strains producing high value biologics ...
... To get 1kg of osteopontin from algae a company would only need to produce 100kg of biomass • Algae biotechnology opens up new opportunities in health and nutrition • Advances made by algae cultivators can be applied to algal strains producing high value biologics ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... studied activeimmunization using whole cells or selected cell envelope components and suggested that the murine model would beuseful for investigating the tissue-destructive components of P.gingivalis18 Bottlenecks In Periodontal Vaccines First of all the complexity of the periodontopathic bacteriam ...
... studied activeimmunization using whole cells or selected cell envelope components and suggested that the murine model would beuseful for investigating the tissue-destructive components of P.gingivalis18 Bottlenecks In Periodontal Vaccines First of all the complexity of the periodontopathic bacteriam ...
Structural biology of bacterial pathogenesis
... [43]. Secretion through the T3SS requires specific chaperones that recognize an N-terminal chaperone-binding domain in the effector protein. Remarkably, structures of the chaperone-binding domains of the Salmonella and Yersinia effectors SptP and YopE bound to their respective chaperones, SicP and S ...
... [43]. Secretion through the T3SS requires specific chaperones that recognize an N-terminal chaperone-binding domain in the effector protein. Remarkably, structures of the chaperone-binding domains of the Salmonella and Yersinia effectors SptP and YopE bound to their respective chaperones, SicP and S ...
1055 BIOTECHNOLOGYDERIVED ARTICLES—PEPTIDE
... avoid its contribution to the chromatographic map pattern. A proteintoprotease ratio between 20:1 and 200:1 is generally used. It is recommended that the cleavage agent be added in two or more stages to optimize cleavage. Nonetheless, the final reaction volume remains small enough to facilitate th ...
... avoid its contribution to the chromatographic map pattern. A proteintoprotease ratio between 20:1 and 200:1 is generally used. It is recommended that the cleavage agent be added in two or more stages to optimize cleavage. Nonetheless, the final reaction volume remains small enough to facilitate th ...
Structure analysis of DNA relaxases, the key enzymes of bacterial
... were expressed as 6×His-tagged fusions and purified. Small-angle X-ray scattering and chemical cross-linking proved that TraAN246 and TraA form dimers in solution. Both proteins revealed oriTpIP501 cleavage activity on supercoiled plasmid DNA in vitro. oriT binding was demonstrated by electrophoreti ...
... were expressed as 6×His-tagged fusions and purified. Small-angle X-ray scattering and chemical cross-linking proved that TraAN246 and TraA form dimers in solution. Both proteins revealed oriTpIP501 cleavage activity on supercoiled plasmid DNA in vitro. oriT binding was demonstrated by electrophoreti ...
Identification of caspase-1 activating factor of Burkholderia
... • Huge genome: 4.1Mb +3.2Mb • Many virulence factors: siderophore, flagella, T3SSs, T6SSs • Able to invade cells and survive intracellularly ...
... • Huge genome: 4.1Mb +3.2Mb • Many virulence factors: siderophore, flagella, T3SSs, T6SSs • Able to invade cells and survive intracellularly ...
Will Koning`s Summer Project
... I attempted to clone and express the putative IL-10 proteins from L. gasseri and L. johnsonii to test if the display functional similarity and could be suppressing any inflammatory response thus explaining how these bacteria can live on our epithelial cells without causing inflammation. I also inves ...
... I attempted to clone and express the putative IL-10 proteins from L. gasseri and L. johnsonii to test if the display functional similarity and could be suppressing any inflammatory response thus explaining how these bacteria can live on our epithelial cells without causing inflammation. I also inves ...
Characterization of the Bat proteins in the oxidative stress
... representative images in Figure 4A, no morphological or ultrastructural differences were observed between the WT and mutant strains by any of these analyses. Growth rates of WT, ΔbatA, and ΔbatABD strains were compared during in vitro cultivation in EMJH liquid medium and also for colony formation o ...
... representative images in Figure 4A, no morphological or ultrastructural differences were observed between the WT and mutant strains by any of these analyses. Growth rates of WT, ΔbatA, and ΔbatABD strains were compared during in vitro cultivation in EMJH liquid medium and also for colony formation o ...
Escherichia coli
... The aim of the project was to investigate the use of Lacritin Lacritin targets cell-to-cell communication and the extrato inhibit biofilm formation rather than prevent biofilm cellular matrix of bacterial cells, qualities that have been formation. proposed as enabling biofilm antibacterial resistanc ...
... The aim of the project was to investigate the use of Lacritin Lacritin targets cell-to-cell communication and the extrato inhibit biofilm formation rather than prevent biofilm cellular matrix of bacterial cells, qualities that have been formation. proposed as enabling biofilm antibacterial resistanc ...
THE GENUS CLOSTRIDUM
... – Polysaccharide capsule is only rarely found on cells. – The peptidoglycan layer is the major structural component ...
... – Polysaccharide capsule is only rarely found on cells. – The peptidoglycan layer is the major structural component ...
Membrane-associated and Solubilized ATPases of Streptococcus
... membrane-bound ATPases of S. mutmw, S. saizguis, and S . fclecalis was examined. Thesc enzymes appear to be important for acidurancc of whole cells and for cytoplasmic pH regulation (Kobayashi, 1985; Bender et d . , 1986). The oxybiontic bacterium E. coli generates a proton gradient through respirat ...
... membrane-bound ATPases of S. mutmw, S. saizguis, and S . fclecalis was examined. Thesc enzymes appear to be important for acidurancc of whole cells and for cytoplasmic pH regulation (Kobayashi, 1985; Bender et d . , 1986). The oxybiontic bacterium E. coli generates a proton gradient through respirat ...
The Development and Optimization of a Sensitive and Specific
... development of a qPCR assay, which is highly sensitive and specific for detecting B. lonestari. Using the programs ClustalW and GeneDoc, a unique region between 594–719bp in the B. lonestari flagellin gene was identified and primers and a molecular Beacon probe were designed. A plasmid containing th ...
... development of a qPCR assay, which is highly sensitive and specific for detecting B. lonestari. Using the programs ClustalW and GeneDoc, a unique region between 594–719bp in the B. lonestari flagellin gene was identified and primers and a molecular Beacon probe were designed. A plasmid containing th ...
Staphylococcus aureus
... located on the cell surface but is also released into the culture medium during the cell growth. A unique property of protein A is its ability to bind to the Fc part of all IgG molecules except IgG3. It is not an antigen-antibody specific reaction. ...
... located on the cell surface but is also released into the culture medium during the cell growth. A unique property of protein A is its ability to bind to the Fc part of all IgG molecules except IgG3. It is not an antigen-antibody specific reaction. ...
Frequent gene fissions associated with human pathogenic bacteria
... 2.2. Classification of the events based on evolutionary analysis identifies many fissions and multiple events in pathogenic bacteria Each one of the 141 reference proteins represents a different event which, based on the results of the reverse BLAST could be classified as a unique fusion or fission event ...
... 2.2. Classification of the events based on evolutionary analysis identifies many fissions and multiple events in pathogenic bacteria Each one of the 141 reference proteins represents a different event which, based on the results of the reverse BLAST could be classified as a unique fusion or fission event ...
Glycerol 3-phosphate Oxidase (G9637) - Datasheet - Sigma
... is oxidized to enter the glycolytic pathway. -Glycerophosphate oxidase (GPO), a dimeric protein, catalyzes the oxidation of -glycerol-3-phosphate to dihydroxyacetone phosphate by the following reaction: GPO ...
... is oxidized to enter the glycolytic pathway. -Glycerophosphate oxidase (GPO), a dimeric protein, catalyzes the oxidation of -glycerol-3-phosphate to dihydroxyacetone phosphate by the following reaction: GPO ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... clinicaldiagnosis of UTI cases using dot blot. Urine sampleswithresultsgroupedinto positive and negativebolt bot urine cultures as comparedwith the gold standard.The results of the study also show that the molecular weight of OMP A.baumannii 57.2 kDa responded to s-IgA. An examination was made of th ...
... clinicaldiagnosis of UTI cases using dot blot. Urine sampleswithresultsgroupedinto positive and negativebolt bot urine cultures as comparedwith the gold standard.The results of the study also show that the molecular weight of OMP A.baumannii 57.2 kDa responded to s-IgA. An examination was made of th ...
Members of the IclR family of bacterial transcriptional regulators
... Members of the IclR family of regulators are proteins with around 250 residues. The IclR family is best defined by a profile covering the effector binding domain. This is supported by structural data and by a number of mutants showing that effector specificity lies within a pocket in the C-terminal ...
... Members of the IclR family of regulators are proteins with around 250 residues. The IclR family is best defined by a profile covering the effector binding domain. This is supported by structural data and by a number of mutants showing that effector specificity lies within a pocket in the C-terminal ...
〈1055〉 biotechnology- derived articles—peptide mapping
... to avoid its contribution to the chromatographic map pattern. A protein-to-protease ratio between 20 : 1 and 200 : 1 is generally used. It is recommended that the cleavage agent be added in two or more stages to optimize cleavage. Nonetheless, the final reaction volume remains small enough to facili ...
... to avoid its contribution to the chromatographic map pattern. A protein-to-protease ratio between 20 : 1 and 200 : 1 is generally used. It is recommended that the cleavage agent be added in two or more stages to optimize cleavage. Nonetheless, the final reaction volume remains small enough to facili ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.