Crosslinking Technical Handbook
... in the same manner as EDC. However, because DCC is not aqueous-soluble, it is primarily used in manufacturing and organic synthesis applications rather than in the typical protein research biology lab. For example, most commercially available, ready-to-use NHS ester crosslinkers and labeling reagent ...
... in the same manner as EDC. However, because DCC is not aqueous-soluble, it is primarily used in manufacturing and organic synthesis applications rather than in the typical protein research biology lab. For example, most commercially available, ready-to-use NHS ester crosslinkers and labeling reagent ...
Recombinase-based in vivo expression technology identifies a
... primarily recognized as a pathogen, S. pyogenes also colonizes the skin and throat often without causing disease, and while numerous surface adhesions are important to attach to these surfaces, additional factors important for colonization and persistence by S. pyogenes are poorly understood. In add ...
... primarily recognized as a pathogen, S. pyogenes also colonizes the skin and throat often without causing disease, and while numerous surface adhesions are important to attach to these surfaces, additional factors important for colonization and persistence by S. pyogenes are poorly understood. In add ...
Multi-domain protein families and domain pairs
... form the set of proteins in a genome. Most proteins in both prokaryote and eukaryote genomes consist of two or more domains, and we show that the family size distribution of multi-domain protein families follows a power law like that of individual families. Most domain pairs occur in four to six dif ...
... form the set of proteins in a genome. Most proteins in both prokaryote and eukaryote genomes consist of two or more domains, and we show that the family size distribution of multi-domain protein families follows a power law like that of individual families. Most domain pairs occur in four to six dif ...
Str. pyogenes
... They are found in hair-like projections of the streptococcal surface, protrudes from the outer surface ,determine virulence. It interferes with ingestion by phagocytes, i.e., it is antiphagocytic. Antibody to M protein provides type-specific immunity. It’s a major virulent factor for group A strepto ...
... They are found in hair-like projections of the streptococcal surface, protrudes from the outer surface ,determine virulence. It interferes with ingestion by phagocytes, i.e., it is antiphagocytic. Antibody to M protein provides type-specific immunity. It’s a major virulent factor for group A strepto ...
Identification and partial characterization of acid Haemophilus parasuis
... ANNEX 6. REPORT OF THE MALDI-TOF ANALYSIS PERFORMED TO PROTEINS 222, 223 AND ...
... ANNEX 6. REPORT OF THE MALDI-TOF ANALYSIS PERFORMED TO PROTEINS 222, 223 AND ...
Cellular Adjustments of Bacillus subtilis and Other
... are killed upon a severe osmotic down-shock (Hoffmann et al. 2008). Indeed, Wahome and Setlow (2008) have shown that the overproduction of the YkuT protein in an mscL mutant background can rescue the osmotically down-shocksensitive phenotype of a B. subtilis mscL mutant strain, thereby providing fur ...
... are killed upon a severe osmotic down-shock (Hoffmann et al. 2008). Indeed, Wahome and Setlow (2008) have shown that the overproduction of the YkuT protein in an mscL mutant background can rescue the osmotically down-shocksensitive phenotype of a B. subtilis mscL mutant strain, thereby providing fur ...
demonstration of novel enoyl-acyl carrier protein
... NADH-quinone reductases. Comparing the presence of conserved domains present in all known ENRs (Marchler-Bauer, Lu et al. 2011) suggests that the ENR activity may result from convergent evolution involving protein domain shuffling. We conclude with preliminary data suggesting the existence of other ...
... NADH-quinone reductases. Comparing the presence of conserved domains present in all known ENRs (Marchler-Bauer, Lu et al. 2011) suggests that the ENR activity may result from convergent evolution involving protein domain shuffling. We conclude with preliminary data suggesting the existence of other ...
Electron Cryomicroscopic Visualization of PomA/B
... the shorter domain is most likely the PomB N-terminal side. Deletion of peptidoglycan-binding motif We also examined a mutant with deletion of the C-terminal 120 residues of PomB, which includes the peptidoglycan-binding motif (PomA/BDC).43 This mutant protein was purified in the same way without an ...
... the shorter domain is most likely the PomB N-terminal side. Deletion of peptidoglycan-binding motif We also examined a mutant with deletion of the C-terminal 120 residues of PomB, which includes the peptidoglycan-binding motif (PomA/BDC).43 This mutant protein was purified in the same way without an ...
The Comprehensive Sourcebook of Bacterial
... any amino acid, and U standing for any large hydrophobic amino acid such as L, V, I, F, or Y). This so-called RTX motif, which defines this class of cytotoxins, constitutes a specific calcium-binding sequence [4,5]. Indeed, most cytotoxins of the HlyA family are calcium-dependent [1–3], and few of t ...
... any amino acid, and U standing for any large hydrophobic amino acid such as L, V, I, F, or Y). This so-called RTX motif, which defines this class of cytotoxins, constitutes a specific calcium-binding sequence [4,5]. Indeed, most cytotoxins of the HlyA family are calcium-dependent [1–3], and few of t ...
Catabolite repression and inducer control in Gram
... PTS activity on the basis of in vitro studies (Deutscher e t al., 1984), but the physiological significance of this observation was until recently questioned, due to limited in vivo studies conducted with Bacillzls szlbtilis and Stapbylococczls azlrezls (Reizer e t al., 1989a; Sutrina e t al., 1990; ...
... PTS activity on the basis of in vitro studies (Deutscher e t al., 1984), but the physiological significance of this observation was until recently questioned, due to limited in vivo studies conducted with Bacillzls szlbtilis and Stapbylococczls azlrezls (Reizer e t al., 1989a; Sutrina e t al., 1990; ...
Processing of lysozyme at distinct loops by pepsin: A novel action for
... using band intensity of control protein (treated without pepsin) and standard molecular weight markers. Percent proteolysis was calculated by dividing the optical density of the librated peptide bands in a lane containing Ppn-Lz by the optical density of the total protein bands. Peptides immobilized ...
... using band intensity of control protein (treated without pepsin) and standard molecular weight markers. Percent proteolysis was calculated by dividing the optical density of the librated peptide bands in a lane containing Ppn-Lz by the optical density of the total protein bands. Peptides immobilized ...
13 Immunological Methods for the Detection Campylobacter and Potential Use in Biosensors
... on the membrane. Two clear lines on the membrane are a positive result. A single line in the control zone is a negative result. Lateral flow immunoassays have many advantages including their simplicity, production of a result within 15 minutes, stability with a long shelf life even in some cases wit ...
... on the membrane. Two clear lines on the membrane are a positive result. A single line in the control zone is a negative result. Lateral flow immunoassays have many advantages including their simplicity, production of a result within 15 minutes, stability with a long shelf life even in some cases wit ...
SP-A - e
... The above variants have been shown to exhibit qualitative and quantitative differences. In addition environmental insults, such as ozone, appear to differentially alter their functional activity and thus contribute to individual differences in disease susceptibility. ...
... The above variants have been shown to exhibit qualitative and quantitative differences. In addition environmental insults, such as ozone, appear to differentially alter their functional activity and thus contribute to individual differences in disease susceptibility. ...
Microspectrometric insights on the uptake of antibiotics
... A key unmet point is the ability to measure the quantity of antibiotic inside the bacterium. Several experimental technologies have been developed to reach this goal, for example: bioassays, fluorimetric detection, radiometry, and HPLC. These techniques, although having important benefits, fail to g ...
... A key unmet point is the ability to measure the quantity of antibiotic inside the bacterium. Several experimental technologies have been developed to reach this goal, for example: bioassays, fluorimetric detection, radiometry, and HPLC. These techniques, although having important benefits, fail to g ...
Quantitative Comparison of the Hydrogen Bond
... globular than the native state. Nevertheless, certain secondary elements can be clearly detected. In the N-terminal part, the first antiparallel β-hairpin β1/β2 (residues M1 to V17) and the central R-helix R (residues I23 to G35) of the native state preserve their secondary conformation in the A-sta ...
... globular than the native state. Nevertheless, certain secondary elements can be clearly detected. In the N-terminal part, the first antiparallel β-hairpin β1/β2 (residues M1 to V17) and the central R-helix R (residues I23 to G35) of the native state preserve their secondary conformation in the A-sta ...
Platsaki, S. 2015 (12mth) - Newcastle University eTheses
... novel copper protein, Csp1, was identified. Two more homologues, Csp2 and Csp3, were identified in M. trichosporium OB3b through bioinformatics. In vitro characterization of Csp1 and the homologue Csp3 showed these proteins are tetramers of 4-helix bundles that bind 13 and 18 Cu(I) ions per monomer, ...
... novel copper protein, Csp1, was identified. Two more homologues, Csp2 and Csp3, were identified in M. trichosporium OB3b through bioinformatics. In vitro characterization of Csp1 and the homologue Csp3 showed these proteins are tetramers of 4-helix bundles that bind 13 and 18 Cu(I) ions per monomer, ...
Genomic and functional adaptation in surface ocean
... the cosmopolitan taxa, implying that these groups, as judged by 16S rRNA sequence, are internally extremely diverse. The GOS data set was also remarkable because of its apparent lack of relatedness to the entire collection of sequenced genomes: using a process called fragment recruitment, which is a ...
... the cosmopolitan taxa, implying that these groups, as judged by 16S rRNA sequence, are internally extremely diverse. The GOS data set was also remarkable because of its apparent lack of relatedness to the entire collection of sequenced genomes: using a process called fragment recruitment, which is a ...
Bacterial Symbioses and the Innate Immune Response of the Model
... research for the past seven years. I am also grateful to the members of my committee, Dr. Joerg Graf, Dr. Daniel Gage, Dr. David Benson and Dr. Steven Geary, who asked held me to a high standard and asked really good questions. Past and present members of the Nyholm Lab profoundly affected my develo ...
... research for the past seven years. I am also grateful to the members of my committee, Dr. Joerg Graf, Dr. Daniel Gage, Dr. David Benson and Dr. Steven Geary, who asked held me to a high standard and asked really good questions. Past and present members of the Nyholm Lab profoundly affected my develo ...
by Matthew Patrick Mc Cusker B.A.(Mod) in Microbiology ,1995
... Lipophilic and amphipathic antibiotics enter the cell through the hydrophobic pathway by passive permeation through the outer membrane bilayer (Hancock & Bell, 1988). The mechanism of uptake of hydrophobic and amphipathic antibiotics is not well understood but probably involves the partitioning into ...
... Lipophilic and amphipathic antibiotics enter the cell through the hydrophobic pathway by passive permeation through the outer membrane bilayer (Hancock & Bell, 1988). The mechanism of uptake of hydrophobic and amphipathic antibiotics is not well understood but probably involves the partitioning into ...
Spirulina
... In Mexico, Spirulina was found to be harvested and dried for consumption up until the late 16th century The Spanish Conquest led to lakes being drained to make room for new civilization (2) ...
... In Mexico, Spirulina was found to be harvested and dried for consumption up until the late 16th century The Spanish Conquest led to lakes being drained to make room for new civilization (2) ...
Contribution of the outer surface proteins of Borrelia
... The first likely case of Lyme disease to be documented was described by Buchwald in Germany , who in 1883 reported on a patient with an idiopathic skin atrophy (Buchwald, 1883). In 1902 similar cases were described, and the disease was named acrodermatitis chronica atrophicans, ACA (Herxheimer and H ...
... The first likely case of Lyme disease to be documented was described by Buchwald in Germany , who in 1883 reported on a patient with an idiopathic skin atrophy (Buchwald, 1883). In 1902 similar cases were described, and the disease was named acrodermatitis chronica atrophicans, ACA (Herxheimer and H ...
CLONING AND EXPRESSION OF PLASMIDS ENCODING
... (Subbalakshmi and Sitaram, 1998). Direct interaction with the lipid bilayer was hypothesized after replacing L-amino acids with all D enantiomers. This did not inhibit membrane binding due to stereospecific protein receptors as previously thought (Huang et al, 2000). Several peptides can influence m ...
... (Subbalakshmi and Sitaram, 1998). Direct interaction with the lipid bilayer was hypothesized after replacing L-amino acids with all D enantiomers. This did not inhibit membrane binding due to stereospecific protein receptors as previously thought (Huang et al, 2000). Several peptides can influence m ...
foldpat20-text-092101-dg-jl
... We carried out a structural-genomics analysis of the folds and structural superfamilies in the first 20 completely sequenced genomes, focusing on the patterns of fold usage and trying to identify structural characteristics of typical and atypical folds. We assigned folds to sequences using PSI-blast ...
... We carried out a structural-genomics analysis of the folds and structural superfamilies in the first 20 completely sequenced genomes, focusing on the patterns of fold usage and trying to identify structural characteristics of typical and atypical folds. We assigned folds to sequences using PSI-blast ...
Structural Genomics Analysis
... We carried out a structural-genomics analysis of the folds and structural superfamilies in the first 20 completely sequenced genomes, focusing on the patterns of fold usage and trying to identify structural characteristics of typical and atypical folds. We assigned folds to sequences using PSI-blast ...
... We carried out a structural-genomics analysis of the folds and structural superfamilies in the first 20 completely sequenced genomes, focusing on the patterns of fold usage and trying to identify structural characteristics of typical and atypical folds. We assigned folds to sequences using PSI-blast ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.