ArchanaYadav
... of P. multocida have been identified for adhesion, but no single factor could be associated with the virulence of organism. It has been shown that highly virulent type B strains produce hyaluronidase enzyme which contributes significancy in outcome of infection. Bivalent cation like iron has an impo ...
... of P. multocida have been identified for adhesion, but no single factor could be associated with the virulence of organism. It has been shown that highly virulent type B strains produce hyaluronidase enzyme which contributes significancy in outcome of infection. Bivalent cation like iron has an impo ...
Ligand-responsive Transcriptional Regulation by Members of the
... before the first codon of marR. The size of each footprint and the fact that each binding site is palindromic with 5 bp half-sites separated by 2 bp is consistent with MarR binding as a homodimer at each site, a conclusion supported by size-exclusion chromatography evidence for uncomplexed MarR exis ...
... before the first codon of marR. The size of each footprint and the fact that each binding site is palindromic with 5 bp half-sites separated by 2 bp is consistent with MarR binding as a homodimer at each site, a conclusion supported by size-exclusion chromatography evidence for uncomplexed MarR exis ...
Determinants of pathogenicity and avirulence in plant pathogenic
... proteins should promote searches for additional avr-like genes by providing an assay that can be performed in culture and is independent of the requirement for test plants that happen to have a corresponding R gene, and it will enable direct investigation of Avr targeting signals and secretion mecha ...
... proteins should promote searches for additional avr-like genes by providing an assay that can be performed in culture and is independent of the requirement for test plants that happen to have a corresponding R gene, and it will enable direct investigation of Avr targeting signals and secretion mecha ...
Elaheh Gholami Parizad.pmd
... compounds and the mechanisms. In these bacteria, materials must pass through both the inner and outer membranes or certain substances should enter into the host cell and therefore various molecules and mechanisms are required for this purpose. Gram-positive bacteria are common by Gram-negative bacte ...
... compounds and the mechanisms. In these bacteria, materials must pass through both the inner and outer membranes or certain substances should enter into the host cell and therefore various molecules and mechanisms are required for this purpose. Gram-positive bacteria are common by Gram-negative bacte ...
Isolation of casein from milk
... • β- casein it is more hydrophobic than α-caseins and κ-casein • Because α-caseins and β-caseins are highly phosphorylated, they are very sensitive to the concentration of calcium salts, that is, they will precipitate with excess Ca2+ ions ...
... • β- casein it is more hydrophobic than α-caseins and κ-casein • Because α-caseins and β-caseins are highly phosphorylated, they are very sensitive to the concentration of calcium salts, that is, they will precipitate with excess Ca2+ ions ...
The Discovery and Application of Bacteriophage Receptor Binding
... for pathogen detection through the attachment of RBPs to surfaces or beads. This thesis describes new techniques that take advantage of these proteins that act as surrogate antibodies. Recent years have seen a resurgence in bacteriophage research, often in the form of phage therapy, due to the recen ...
... for pathogen detection through the attachment of RBPs to surfaces or beads. This thesis describes new techniques that take advantage of these proteins that act as surrogate antibodies. Recent years have seen a resurgence in bacteriophage research, often in the form of phage therapy, due to the recen ...
Light Forge: A Microfluidic High Throughput Platform for Rapid and
... accelerate protein folding, promote secondary structure formation, reduce protein aggregation, shield hydrophobic surfaces, promote disulfide pairing, and increase folding cooperativity. It is well known that gp120 can accommodate a remarkable heterogeneity in terms of the number and location of gly ...
... accelerate protein folding, promote secondary structure formation, reduce protein aggregation, shield hydrophobic surfaces, promote disulfide pairing, and increase folding cooperativity. It is well known that gp120 can accommodate a remarkable heterogeneity in terms of the number and location of gly ...
Isolation and expression of recombinant antibody
... complications with common epitope tags used for detection of scFv. These problems were successfully addressed by converting the scFv into the single chain antibody fragment (scAb) format (McGregor et al., 1994; Hayhurst, 2000) and co-expression of the chaperone Skp (Bothmann and Pluckthun, 1998; Hay ...
... complications with common epitope tags used for detection of scFv. These problems were successfully addressed by converting the scFv into the single chain antibody fragment (scAb) format (McGregor et al., 1994; Hayhurst, 2000) and co-expression of the chaperone Skp (Bothmann and Pluckthun, 1998; Hay ...
Comparative genomics of regulation of heavy
... transcription of the mer genes needed for the detoxification of mercury. In Gram-negative bacteria, these two promoters are directly adjacent in the divergent orientation. MerR binds in between so that it contacts both promoters. Activation of transcription from the Pt promoter occurs in the presenc ...
... transcription of the mer genes needed for the detoxification of mercury. In Gram-negative bacteria, these two promoters are directly adjacent in the divergent orientation. MerR binds in between so that it contacts both promoters. Activation of transcription from the Pt promoter occurs in the presenc ...
Striped murrel S1 family serine protease: immune
... which rank among the most biologically vital and widely distributed enzyme. They have been categorized into non-clip domain SPs (or single domain) and clip domain SPs based on the presence of the domain. These SPs are multigene encoded protein family sharing a common catalytic mechanism along with s ...
... which rank among the most biologically vital and widely distributed enzyme. They have been categorized into non-clip domain SPs (or single domain) and clip domain SPs based on the presence of the domain. These SPs are multigene encoded protein family sharing a common catalytic mechanism along with s ...
Scalable graphene field-effect sensors for specific protein detection
... Figure 2, upper panel, shows Isd (Vlg ) for a set of three bare graphene devices. The curves are representative of the typical variability that we observe between different devices. The highest currents correspond to a graphene sheet resistance of ∼2 k/sq, which is typical for moderately doped sing ...
... Figure 2, upper panel, shows Isd (Vlg ) for a set of three bare graphene devices. The curves are representative of the typical variability that we observe between different devices. The highest currents correspond to a graphene sheet resistance of ∼2 k/sq, which is typical for moderately doped sing ...
Poster
... carbohydrates and sialic acid within the neural plasma membrane. The sialic acid region of the ganglioside binds with specific residues on the BoNT GBP2: Tyr1115, Ser1275, Ile1240, Tyr1243, and Ser1242, modeled by the Cudahy SMART (Students Modeling A Research Topic) Team using 3D printing technolog ...
... carbohydrates and sialic acid within the neural plasma membrane. The sialic acid region of the ganglioside binds with specific residues on the BoNT GBP2: Tyr1115, Ser1275, Ile1240, Tyr1243, and Ser1242, modeled by the Cudahy SMART (Students Modeling A Research Topic) Team using 3D printing technolog ...
Evolutionary relationships among photosynthetic
... The use of signature sequences for deducting the branching order of bacterial groups The usefulness of a conserved indel for understanding evolutionary relationship is based on the rationale that if a conserved indel of defined length and sequence is found at the same position in a given protein (or ...
... The use of signature sequences for deducting the branching order of bacterial groups The usefulness of a conserved indel for understanding evolutionary relationship is based on the rationale that if a conserved indel of defined length and sequence is found at the same position in a given protein (or ...
Lab.3 Isolation of casein from milk
... κ-casein • Because α-caseins and β-caseins are highly phosphorylated, they are very sensitive to the concentration of calcium salts, that is, they will precipitate with excess Ca2+ ions ...
... κ-casein • Because α-caseins and β-caseins are highly phosphorylated, they are very sensitive to the concentration of calcium salts, that is, they will precipitate with excess Ca2+ ions ...
The_Human_Microbiome_Congress_
... Adaptation of the human gut microbiome to its unique environment is typically described in terms of the relative composition and diversity of microbial species. An alternative (and complementary) perspective can provided by analyzing the relative distribution of protein families in metagenomic datas ...
... Adaptation of the human gut microbiome to its unique environment is typically described in terms of the relative composition and diversity of microbial species. An alternative (and complementary) perspective can provided by analyzing the relative distribution of protein families in metagenomic datas ...
Pedro Miguel Azevedo Veloso Improving derived Listeria phage
... activity improvements of endolysins in food storage conditions. The experimental work developed in the scope of this thesis aimed at directing endolysin activity towards refrigeration temperatures against L. monocytogenes through the use of directed evolution strategies – error-prone PCR and cryodri ...
... activity improvements of endolysins in food storage conditions. The experimental work developed in the scope of this thesis aimed at directing endolysin activity towards refrigeration temperatures against L. monocytogenes through the use of directed evolution strategies – error-prone PCR and cryodri ...
Application of comparative genomics in the identification and
... prokaryotes. As a result of this procedure we were able to recover two widespread families of 7 TM receptors in bacteria that are distantly related to the eukaryotic 7 TM receptors and prokaryotic rhodopsins. Using sequence profile analysis, we were able to establish that the first members of these ...
... prokaryotes. As a result of this procedure we were able to recover two widespread families of 7 TM receptors in bacteria that are distantly related to the eukaryotic 7 TM receptors and prokaryotic rhodopsins. Using sequence profile analysis, we were able to establish that the first members of these ...
Elongation Factor P
... Bacterial elongation factor P (EF-P) is a poorly understood soluble protein that has been shown to enhance the first step of peptide bond formation through an interaction with the ribosome and initiator tRNA. The crystal structure of EF‐P shows that EF‐P mimics the tRNA shape. Orthologous proteins h ...
... Bacterial elongation factor P (EF-P) is a poorly understood soluble protein that has been shown to enhance the first step of peptide bond formation through an interaction with the ribosome and initiator tRNA. The crystal structure of EF‐P shows that EF‐P mimics the tRNA shape. Orthologous proteins h ...
Regulation of OmpA and Its Effect on Shigella Virulence
... million fatalities each year. With growing antibiotic resistance and no vaccine, new methods must be developed to treat Shigellosis. Outer membrane protein A, or OmpA, is required for Shigella virulence and may be a good target for inhibitory drugs. Before these new treatments can be developed, we n ...
... million fatalities each year. With growing antibiotic resistance and no vaccine, new methods must be developed to treat Shigellosis. Outer membrane protein A, or OmpA, is required for Shigella virulence and may be a good target for inhibitory drugs. Before these new treatments can be developed, we n ...
Neisseria - Caangay.com
... – Mediates binding to epithelial cells – Opaque colonies – Adherence of bacteria to each other and to eukaryotic cell ...
... – Mediates binding to epithelial cells – Opaque colonies – Adherence of bacteria to each other and to eukaryotic cell ...
Characterization of Five Brevibacillus Bacteriophages and Their
... from honey of infected hives (Gillard, 2008 and Pohorecka, 2008). Endospores are able to withstand harsh treatments used to isolate them. Most hives are considered to be latent carriers of the endospores and any effective eradication of the pathogen must address the presence of endospores (Genersch ...
... from honey of infected hives (Gillard, 2008 and Pohorecka, 2008). Endospores are able to withstand harsh treatments used to isolate them. Most hives are considered to be latent carriers of the endospores and any effective eradication of the pathogen must address the presence of endospores (Genersch ...
Prescott 6e Chapter 12 - McGraw Hill Higher Education
... arating the segments coding for individual polypeptides. Polygenic messenger polypeptides usually function together in some way (e.g., as part of the same metabolic pathway). At the 3′ end, following the last termination codon, is a nontranslated trailer. Messenger RNA is synthesized under the direc ...
... arating the segments coding for individual polypeptides. Polygenic messenger polypeptides usually function together in some way (e.g., as part of the same metabolic pathway). At the 3′ end, following the last termination codon, is a nontranslated trailer. Messenger RNA is synthesized under the direc ...
Mechanism of Action of the Novel Aminomethylcycline Antibiotic
... purified by nickel-affinity chromatography (10) and added in a 1:1 molar ratio with ribosomes as appropriate. Poly(Phe) chains were precipitated by adding 100 ml of 1% bovine serum albumin and 2 ml of 5% trichloroacetic acid (TCA) and incubated at 90°C for 15 min. The mixture was filtered through gl ...
... purified by nickel-affinity chromatography (10) and added in a 1:1 molar ratio with ribosomes as appropriate. Poly(Phe) chains were precipitated by adding 100 ml of 1% bovine serum albumin and 2 ml of 5% trichloroacetic acid (TCA) and incubated at 90°C for 15 min. The mixture was filtered through gl ...
Compartmentalization of phage 29 DNA replication: interaction
... replicating DNA, as well as the transcriptional and translational machinery, have speci®c subcellular localizations (Lemon and Grossman, 1998; Lewis et al., 2000). Direct visualization of the replicative DNA polymerase in living B.subtilis cells has revealed that it is localized at discrete intracel ...
... replicating DNA, as well as the transcriptional and translational machinery, have speci®c subcellular localizations (Lemon and Grossman, 1998; Lewis et al., 2000). Direct visualization of the replicative DNA polymerase in living B.subtilis cells has revealed that it is localized at discrete intracel ...
Native Bacillus thermoproteolyticus Thermolysin
... the Gram-positive bacteria Bacillus thermoproteolyticus. It requires one zinc ion for enzyme activity and four calcium ions for structural stability. Thermolysin specifically catalyzes the hydrolysis of peptide bonds containing hydrophobic amino acids. However thermolysin is also widely used for pep ...
... the Gram-positive bacteria Bacillus thermoproteolyticus. It requires one zinc ion for enzyme activity and four calcium ions for structural stability. Thermolysin specifically catalyzes the hydrolysis of peptide bonds containing hydrophobic amino acids. However thermolysin is also widely used for pep ...
Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. The differing conditions that can be examined are very diverse, e.g. pH, salts, additives, drugs, drug leads, oxidation/reduction, or mutations. The binding of low molecular weight ligands can increase the thermal stability of a protein, as described by Koshland (1958) and Linderstrom-Lang and Schellman (1959). Almost half of enzymes require a metal ion co-factor. Thermostable proteins are often more useful than their non-thermostable counterparts, e.g. DNA polymerase in the polymerase chain reaction, so protein engineering often includes addingmutations to increase thermal stability. Protein crystallisation is more successful for proteins with a higher melting point and adding buffer components that stabilise proteins improve the likelihood of protein crystals forming.If examining pH then the possible effects of the buffer molecule on thermal stability should be taken into account along with the fact that pKa of each buffer molecule changes uniquely with temperature. Additionally, any time a charged species is examined the effects of the counterion should be accounted for.Thermal stability of proteins has traditionally been investigated using biochemical assays, circular dichroism, or differential scanning calorimetry. Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and differential scanning calorimetry both consume large amounts of protein and are low-throughput methods. The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder.