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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2011
BT 1820 - ADVANCED BIOCHEMISTRY
Date : 03-11-2011
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART –A (20 Marks)
Answer all the questions
I. Choose the best answer:
(5x1= 5marks)
What is the concentration of pure water at 250 C?
1)
a. 55.5M
b. 5.55M
c. 555.5M
d. 0.555 M
2) Among the following, which is the second most abundant organic compound on earth?
a. Starch
3)
b. Cellulose
c. Chitin
d. Glycogen
Which is the major site for drug excretion?
a. Liver
4)
b. Kidney
c. Pancreas
Which among the following
ollowing reactions is catalyzed by 3-ketoacyl
3 ketoacyl ACP Synthase?
a. Formation of malonyl CoA
c. Formation of acetyl CoA
5)
d.Blood
b. Formation of acetoacetyl ACP
d. Formation of malonyl ACP
Which Islet of Langerhans cell produces somatostatin ?
a. A cells
b. B cells
c. C cells
d. D cells
II. State whether the following are True or False : if false give reasons
6)
7)
8)
9)
10)
Snake venom does not cause disruption of cell membrane.
Osmotic pressure is directly proportional to concentration of the solute in solution.
β – Oxidation of fatty acids takes place at the cytosol.
Acute liver damage shows high level of alkaline phosphatase.
Antidiuretic hormone is secreted by the anterior pituitary.
III. Complete the following:
11)
12)
13)
14)
15)
(5x1= 5marks)
(5x1= 5marks)
The ability of buffers to tolerate pH changes is called _________________.
At lower pH the net charge on glutamate is _______________.
ACP is expanded as _____________.
The chromatographic
ographic separation based on size is _____________________________.
__________ and ____________ secreted by thyroid gland is involved in controlling the metabolism
and heat production.
IV. Answer the following each within 50 words:
(5x1= 5marks)
16)
17)
18)
19)
20)
What is respiratory alkalosis?
Why do fats yield more energy than carbohydrates?
List the substrate level phosphorylation reactions in Glycolysis.
Define Rf.
Give any two examples of tropic hormones.
PART-B
(5x8=40 marks)
V. Answer the following, each within 500 words; Draw diagrams wherever necessary:
21) a. State the properties of water.
(OR)
b. Give any seven classes of organic compounds along with their functional groups.
22) a. Draw the structures of the following molecules.
i) Cholesterol ii) Serine iii) Lactose iv) Cytosine v) Oleic acid vi) Chitin vii) Leucine
(OR)
b. Comment on the optical activity and spectral properties of amino acids.
23) a. Give the complete oxidation of Palmitate.
(OR)
b. List the complexities of Lesch Nyhan syndrome.
24) a. Briefly discuss the principle behind ion exchange chromatography.
(OR)
b. How is drug metabolized in our body?
25)
a. Enumerate the hormones secreted by the anterior and posterior pituitary gland along with their
functions.
(OR)
b. Mention the synthesis and biological action of Prostaglandins.
PART-C
(2x20= 40 marks)
VI. Answer any two of the following, each within 1500 words; Draw diagrams wherever necessary
26) How is pH measured? Explain the working of the pH meter and the factors affecting its measurement.
27) Elaborate the properties of peptide bond and Classify amino acids.
28) Describe the complete oxidation of Glucose and the energy yield associated with it.
29) Outline the various techniques used for the separation of biological molecules and describe in detail
about Gel electrophoresis.
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