Download Immunology

Course Title:
Immunology
Course Number:
Biol-341/541
Semester:
Fall 2013
Location:
HS 268
Time:
8:00-9:30 AM Tue/Thur
Instructor:
Dr. Colleen M. McDermott
Office:
Nursing Ed 101 (424-1217)
E-mail*:
[email protected]
*(I will more rapidly return e-mails, than I will phone calls)
Office Hours:
Tue/Thur 9:30-10:30 AM
Mon, Wed, Fri 11:00 AM-12:00 PM
Required Text:
Owen, Judy, Jenni Punt, and Sharon Stranford.
2012. Kuby-Immunology, 7th. Edition. W.H. Freeman and Co.,
New York.
Grading:
Examinations (100 pts) X 4 = 400
**Case Study (100 pts)
= 100
500
** See Case Study Instruction Page for details
A
AB+
B
BC+
C
CD+
D
DF
=
=
=
=
=
=
=
=
=
=
=
=
100-94
93-90
89-87
86-83
82-80
79-77
76-74
73-70
69-67
66-64
63-60
<60
*Instructor reserves the
right to adjust grades of
the entire class if
necessary (i.e. curve).
There will be extra credit most weeks of the course. Objective Sheets are
provided for topics we will cover and consist of a series of questions pertaining
to the topic. As we finish a topic, answers to the questions posed on the
Objective Sheets will be due the next class period and will be worth up to 1 extra
credit point each (15-20/semester). Extra credit points will be added to the
nearest exam following submission of the Objective Sheet answers.
Completion of the Objective Sheet questions soon after completion of the topic
will allow you to solidify the knowledge you have obtained from that topic. In
addition, it will allow you to organize your thought and put them into writing in
preparation for exams, forming stronger neural connections in your brain than
develop with last minute studying.
Likewise, there will be 3 extra credit points awarded to students who make an
appointment to come to my office (prior to September 28) to discuss the course,
their reasons for taking the course, and their overall interests.
There also may be a few extra credit opportunities linked to the Immuno Portal
available free with your textbook purchase.
IMMUNOLOGY
Biol-341/541
2013
Period
1
2
Date
Sept. 5
Sept. 10
Topic
Introduction
History
Innate vs. adaptive
Inflammation
Humoral vs. cellular
Clonal selection
Reading
Ch. 1 p 1-25
Cells & Organs
Ch. 2 p 27-37
Hematopoiesis
Apoptosis
Lymphoid cells
Myeloid cells
Stromal cells
Growth factors/cytokines
CSF, interleukins
leukemia
Mononuclear cells
Phagocytosis
Antigen-processing
Secretions
Activation
Granulocytic cells
Neutrophils
Eosinophils
Basophils
Mast cells
Dendritic cells
Non-lymphoid
Lymphoid
3
Sept. 12
Cells & Organs, cont.
Ch. 2 p 37-40
Lymphoid Cells
Cluster of differentiation
B cells
Memory cells
Plasma cells
T cells
CD4/TH cells
CD8/TC /CTL cells
MHC restriction
Lymphokines
TS
Null cells
NK cells
AB-dependent cell-mediated
cytotoxicity (ADCC)
4
Sept. 17
5
Sept. 19
6
Sept. 24
Cells & Organs, cont.
Ch. 2 p 41-63
Ch. 14 p 455-467
Primary Lymphoid Organs
Thymus
Stromal cells
Thymocytes
Apoptosis
Bone marrow
Bursa of Fabricius
Secondary Lymphoid Organs
Lymph Nodes
Spleen
Mucosal-associated lymphoid tissue
Lymphocyte recirculation
High endothelial venules
Cell-adhesion molecules
Integrin receptors
Neutrophil Extravasation
Innate Immunity
Ch. 5 p. 141-168
Barriers
Pattern recognition receptors
Soluble molecules
Toll-like receptors
Inflammatory responses
Antigens
Ch. 5 p 175-176
Immunogenicity
Foreignness
Molecular size
Chemical composition
Degradability
Genotype of host
Dosage and administration
Adjuvants
Epitopes
MHC restriction
B-cell epitopes
T-cell epitopes
7
Sept. 26
Antigens, cont.
Haptens
Ch. 17 p. 553-565
Viral antigens
Ch. 4 p. 135, 136
Bacterial antigens Ch. 11 p. 366-368
Mitogens
Superantigens
8
Oct. 1
Immunoglobulins
Ch. 3 p 80-85
Structure
Heavy chain
Light chain
Variable regions
Constant regions
Hinge region
9
Oct. 3
Immunoglobulins,cont Ch. 3 p 85-95
Allotypes
Ch. 13 p. 415-427
Idiotypes
Isotypes
IgG
IgM
IgA
IgE
IgD
Signal Transduction in B cells
Oct. 2-4
EXAMINATION 1-Testing Center
10
Oct. 8
Ag-Ab Interactions
Ch. 20 p. 653-677
Cross-reactivity
Precipitation and Agglutination
Labeled Immunoassays
Monoclonal Antibodies
11
Oct. 10
Ig Genetics
Ch. 7 p 225-247
Heavy chain multi-gene family
Variable region gene rearrangement
V-D-J genes
Allelic exclusion
Class switching
Diversity
12
Oct. 15
MHC
Ch. 8 p 261-298
MHC I molecules
MHC II
MHC III
Haplotypes
MHC and antigen presentation
MHC and infectious disease
13
Oct. 17
T-cell Receptor
Ch. 3 p 95-103
Structure
Ch. 7 p. 247-259
Genetics
V-D-J genes
Diversity
Similarities & Differences with Ig molecules
Accessory molecules
CD2, CD4, CD8
TCR-CD3 complex
Signal transduction
14
Oct. 22
T-cell Maturation
Ch. 9 p. 299-325
Thymic maturation of T-cells
T-cell development
Superantigens
T-cell activation
Co-stimulation
Subpopulations
15
Oct. 24
Cytokines
Ch. 4 p. 105-140
Interleukins
Interferons
TNFs
T-cell activation
B-cell activation
Cytokines and inflammation
Cytokines and disease
Chemokines
16
Oct. 29
Discussion and Review
17
Oct. 31
Humoral Immune Response Ch. 1 p. 17-19
1o vs. 2o response
Ch. 12 p. 385-414
Affinity maturation
Class switching
Antigen presentation by B-cells
Oct. 30-Nov. 1
EXAMINATION 2-Testing Center
18
Nov. 5
Cell-mediated Immun.
Ch. 13 p 427-444
Direct cytotoxicity
Sensitization phase
MLR
Graft vs. host reactions
Effector phase
Mechanisms
Natural killer cells
19
Nov. 7
Cell-mediated Immun.
Ch. 15 p. 506-509
ADCC
Delayed-type hypersensitivity
Transplantation
Ch. 16 p 536-552
Graft Rejection
Transplantation antigens
20
Nov. 12
Complement
Ch. 6 p. 187-200
Components
Activation
Classical pathway
Alternate pathway
Membrane attack complex
21
22
23
Nov. 14
Complement, cont.
Ch. 6 p. 200-223
Regulation
Biological consequences
Cell lysis
Opsonization
Virus neutralization
Complement deficiencies
Nov. 19
Hypersensitivity
Ch.15 p 485-504
IgE-mediated (Type 1)
Antibody-mediated (Type 2)
Nov. 21
Hypersensitivity
Ch. 15 p 504-516
Immune complex (Type 3)
TDTH-mediated (Type 4)
Case Study Pre-Reads Due!
24
Nov. 26
Nov. 28
Exam 3 In class
Thanksgiving Day
25
Dec. 3
Tolerance & Autoimmunity
Ch. 16 p 517-536
Central vs. peripheral tolerance
Diseases
Mechanisms
Therapy
26
Dec. 5
Immunodeficiency
Ch. 18 p 593-626
Phagocyte deficiencies
Humoral deficiencies
Cell-mediated deficiencies
Combined deficiencies
Case Study Due
27
Dec. 10
Review and Discussion
28
Dec. 12
FINAL EXAMINATION- In Class
Students needing extra time to complete in class exams must make
arrangements with instructor prior to examination period. Students unable
to attend in class examinations due to illness or emergency must notify
instructor or departmental office (424-1102) prior to the exam.
Exams 1 and 2 will be given at the Testing Center and may be taken at any
time the Testing Center is open during the designated three day periods.
Photo ID is required to gain entrance to Testing Center.
Examinations will be graded for scientific content, as well as spelling and
grammar. Dictionaries and calculators are permitted during any
examination period (No Scientific or Medical Dictionaries, please)
Additions to Syllabus for Graduate Students (Bio 541- Immunology)
In order to maintain the necessary rigor of the graduate experience, all dual-listed
courses in our department will differ from that taken by an undergraduate.
Specifically, the objectives of the course, the activities undertaken during the
course and the grading scale will differ between the graduate and undergraduate
versions of the course.
1. Objectives for Graduate Students
a.
The graduate students will be expected to evince a greater depth of
knowledge than the undergraduates. This will be demonstrated on
exams and in the case study.
b.
The graduate students will be expected to evince a higher level of
synthesis than the undergraduates. This will be demonstrated in
the extra Graduate Question that will accompany each exam (see
below).
c.
The graduate students will be expected to evince a more
sophisticated level of communication, both oral and written, than
the undergraduates. This will be demonstrated through the essay
exam format used for most of this course, as well as in class
discussions (both small group and whole class).
2. Activities for Graduate Students
Activities not assigned to undergraduates. Graduate
students will answer an additional essay question with each exam.
This question will present data from recent papers in the field and
ask the student to interpret these data or form conclusions from the
data. In addition, the student will be asked to apply this information
to real world problems in immune function, diagnosis, and
immunotherapy.
3. Grading scale for those enrolled for graduate credit is more demanding
than that designed for those enrolled for undergraduate credit.
A = 100-94
A- = 93-90
B+ = 89-87
B = 86-83
B- = 82-80
C+ = 79-77
C = 76-74
F = < 74