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ECOLOGICAL, PHYLOGENETIC AND TAXONOMIC REMARKS ON DIAZOTROPHS AND RELATED GENERA E. Martínez-Romero, J. Caballero-Mellado, B. Gándara1, M. A. Rogel, A. Lopez Merino1, E. T. Wang, L. E. Fuentes-Ramirez, I. Toledo, L. Martinez, I. Hernandez-Lucas, J. MartinezRomero. Centre de Investigatión sobre Fijación de Nitrógeno, UNAM, Cuernavaca, Morelos, México, 1 Escuela Nacional de Ciencias Biológicas, IPN. Mexico 1. Introduction Nitrogen-fixing organisms are very diverse prokaryotes that have been identified scattered in the phylogenetic trees reconstructed from the comparative analysis of ribosomal RNA gene sequences (Martinez-Romero, 1985, Young, 1992). Although 16S rRNA gene-based phytogenies have been criticized and the Universal Tree of Life questioned (Pennisi, 1998), novel approaches from proteome analysis derived from genomes of 20 organisms provide support to the unrooted Universal Tree of Life based on 16S rRNA genes (Tekaia et al., 1999). However, the final answer has not yet been given in this highly controversial field. A critical overview of 16S rRNA gene based phylogenies compared to other markers has been presented (Martinez-Romero et al., 1999). Anyhow, it is clear that nitrogen fixers are found in close relationship to non nitrogen fixers. Nitrogen fixers may constitute clusters restricted in distribution inside groups of non-fixing bacteria as is the case of Acetobacter diazotrophicus and novel Acetobacter N-fixing species inside Acetobacter species (Caballero-Mellado et al., 1999; Fuentes-Ramírez et al, this volume) or Paenibacillus among aerobic endospore-forming Firmicutes (Achouak et al., 1999). In Archea, Nfixation is a general property of the methanogen group and nif genes are found in halophiles, but no fixers have been found in the sulfur dependent archaeobacteria. The Frankia group in the actynomycetes includes atypical strains as well as an infective but no nitrogen fixer isolate (Normand et al., 1996); in Rhizobium there are no nitrogen fixers, nonsymbiotic strains (Segovia et al., 1991), meaning that nitrogen fixation may be easily lost in some cases. The loss of nitrogen fixing ability has been proposed to explain non fixers occurring closely related to N-fixing bacteria. Possessing nitrogen fixing genes would not be advantageous for bacteria if they inhabit N-rich environments. N-fixers are beneficial in plant-bacteria associations, unfortunately, farmers provide N-rich media when heavily fertilizing agricultural fields and we supposed that these practices may negatively affect populations of nitrogen fixers (Martinez-Romero, Caballero-Mellado, 1996). We have documented evidence of different negative effects of fertilization: lack of recovery (FuentesRamírez et al., 1993) and less genetic diversity of A. diazotrophicus in fertilized sugar cane fields (Caballero-Mellado et al., 1995) with diminished colonization by A. diazotrophicus of N-fertilized sugar-cane plants (Fuentes-Ramírez et al., 1999), Fig. 1. We also reported a decrease in the genetic diversity of Rhizobium recovered from P. vulgaris (bean) nodules in fertilized fields using the commonly recommended N dose for bean fertilization in Mexico (Caballero-Mellado, MartinezRomero, 1999), Fig. 2. It seems highly probable that not all nitrogen fixers are known at present. Some may be unculturable. "New findings may fill in some of the blanks on the bacterial map, and the distribution on N2 fixation may prove to be less patchy than it appears at present" (Young, 1992). However, very few real new N-fixers have been reported in these last years, this may be in relation to the fact that the ability to fix nitrogen is not a routine test for new isolates and is performed only in labs specializing in the subject. In addition, pathogenic bacteria are usually excluded from N-fixing analyses as they are supposedly considered non-fixers. Nevertheless, 155 F.O. Pedrosa et al. (eds.), Nitrogen Fixation: From Molecules to Crop Productivity, 155–160. © 2000 Kluwer Academic Publishers. Printed in the Netherlands. 156 Agrobacterium tumefaciens strains, a plant tumor inducer, has nif genes and fixes nitrogen (Kanvinde, Sastry, 1990). Around 30% of klebsiellae, either soil or hospital isolates, are diazotrophic (Postgate, Eady, 1988) or even 100% of them may fix depending on assayed conditions (unpublished results). Furthermore, nitrogen fixation may be expressed only under special bacterial growth conditions. Many free-living organisms require anaerobic conditions for fixation. Bradyrhizobium requires a burst of glutamate; Rhizobium a differentiation process; Azoarcus, diazosome formation (Karg, Reinhold-Hurek, 1996) and in Azospirillum nitrogen fixation is stimulated by plant agglutinins (Karpati et al., 1999). 157 In any case, bioprospection for new fixers should be encouraged and this effort seems highly justified. We are searching for diazotrophs associated to banana plants. The identification of new isolates and the phylogenetical classification that looks toward clustering natural groups are the basis for good science and are clearly related to important changes in family names of N fixers that would be adopted in the new Bergey's Manual. Rhizobiaceae will no longer encompass Rhizobium, Bradyrhizobium and Mesorhizobium species. Mesorhizobium will be included in a new family together with Phyllobacterium and Bradyrhizobium grouped with Rhodopseudomonas in another new family. Azoarcus will be distributed in several families. 2. Rhizobia and related genera From the comparative analysis of 16S rRNA genes, relationships of bacteria to known fixers have been revealed. For example, strains of sewage water Zooglea ramigera were found to be similar to the rhizobia group (Rosselló-Mora et al., 1993); Pseudoaminobacter, Chelatobacter, and Aminobacter with important uses in biotechnology for bioremediation are groups in close relationship to Mesorhizobium spp (Auling et al., 1993; Kampfer et al., 1999); and the bacterialysing Ensifer isolates are similar to (Sind)rhizobium meliloti (D. Balkwill, personal communication). We have found that a new cluster of Mesorhizobium represented by SH0172 (Tan et al., 1999) share with methylotrophic bacteria the capacity to use methanol as sole carbon source. Brucella constitute a cluster of bacteria in relationship to Rhizobium spp (de Ley, 1987; MartinezRomero et al., 1991; Young et al., 1991). From 16S rRNA gene sequences the position of the nodes relating this genus to other rhizobia may not be confidently resolved (Ludwig et al., 1998). By multilocus enzyme electrophoresis we confirmed the close relationship of Brucella and Rhizobium using more than 100 Brucella isolates and Rhizobium reference strains (Fig. 3) and found that Brucella was closely related to R. tropici. All the B. melitensis and B. abortus strains studied have two chromosomes of sizes 2.1 and 1.15 Mb (Michaux et al., 1993). B. suis biovars 2 and 4 have two chromosomes with different sizes and a unique chromosome was found in B. suis biovar 3. An ancestor with a single chromosome has been proposed for all Brucella strains. Rearrangement at the rrn loci led to the existence of two minichromosomes (Jumas-Bilak et al., 1998). Our data clearly support a monochromosomic ancestor of Brucella that must have been related to R. tropici as well. It is interesting to remark that R. tropici is also closely related to Agrobacterium biovar 2 strains and Agrobacterium species have 158 been recovered as clinical isolates in humans (Alnor et al., 1994; Riley, Weaver, 1977). Brucella chromosomes are in the range of size of the megaplasmids of Rhizobium and megaplasmids have been observed in R. tropici but these megaplasmids do not harbor rRNA genes (Geniaux et al., 1995). In addition, R. tropici has smaller plasmids one of them harboring nod and nif genes (Martinez et al., 1987). We are pursuing the analysis of the genetic differences of R. tropici and Brucella towards the elucidation of mechanisms involved in the evolution of a pathogen, bac genes involved in bacteroid differentiation in R. meliloti (Glazebrook et al., 1996) have homologs in Brucella required for macrophage invasion thus providing further support to the close relatedness of both genera. 159 Bioprospection in Rhizobium has yielded fruit. The excellent work in NGR234 led by Broughton (Freiberg et al., 1997; Perret et al., 1999; Pueppke, Broughton, 1999; Viprey et al., 1998) has been possible after the pioneer work of bioprospection of legume symbionts by Trinick in New Guinea in 1965 (Trinick et al., 1980). Similarly, the Rhizobium species described in recent years, such as Azorhizobium caulinodans, Rhizobium galegae, R. fredii, R. etli, and R. tropici, have enriched our knowledge in this field. As an example, novel modifications of Nod factors have been recognized in new species (Bec-Ferté et al., 1994; Poupot et al., 1995; Mergaert et al., 1993) and in isolates as BR816 (Snoeck et al., this volume). BR816 is a Sinorhizobium isolate from the analysis of 16S rRNA sequences (Hernández-Lucas et al., 1995). Naming R. tropici as a new species (MartinezRomero et al., 1991), encouraged research in different aspects including studies that led to the identification of sulfate on Nod factors (Poupot et al., 1993) that was otherwise exclusively encountered in R. meliloti (Roche et al., 1991). R. tropici strains have been extensively used as bean (Phaseolus vulgaris) inoculants in South America (Vlassak et al., 1997). The identification of M. amorpha (Wang et al., 1999a) showed the clear existence of symbiotic plasmids containing nif and nod genes in Mesorhizobium. M. loti and M. plurifarium have these genes in the chromosome and in M. loti, symbiotic islands have been described (Sullivan, Ronson, 1998). This finding adds support to Mesorhizobium being intermediate between Rhizobium (having mainly symbiotic plasmids) and Bradyrhizobium (with symbiotic determinants in chromosome). R. huautleme (Wang et al., 1998) is the sole Rhizobium species related to R. galegae, that was otherwise mainly immersed in an Agrobacterium cluster (Young, Haukka, 1996). The R. huautlense-Sesbania herbacea symbiosis is highly adapted to flooded conditions (Wang, MartinezRomero, submitted) without stem nodules. R. etli represents the main symbiont of Phaseolus in its site of origin (Segovia et al., 1993) . The proposal of a new biovar (mimosae) in R. etli isolates from Mimosa affinis (Wang et al., 1999b) allows the identification of a possible ancestral plasmid of phaseoli and presents clear data on fast plasmidic evolution. It also shows that biovars are a common feature in R. etli, R. leguminosarum, and R. gallicum. 3. 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Biological Nitrogen Fixation pp 4386, Chapman and Hall, New York, USA Young JPW, Haukka KE (1996) New Phytol 133, 87-94 4. Acknowledgments To DGAPA grant IN202097 and to CONACyT 25075-B. To M. Dunn for reading the manuscript.