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Lecture 23 Web: pollev.com/ucibio Text: To: 37607 Type in: 169964 <your question> Nitrogen metabolism How does nitrogen enter/exit? Carbon skeleton source/fate? Regulation? Amino acid catabolism - overview Transfer of Nitrogen to common “carrier” Two “carriers” Getting rid of nitrogen… …as urea Metabolic compartments Like the Urea cycle which of the following also occurs across two cellular compartments? A. TCA B. Glycolysis C. Gluconeogeneis D. Light reactions of photosynthesis E. Beta-oxidation of fats Metabolism is interconnected Dealing with the C-skeletons “Glucogenic”? No net conversion of C from fats Glc. Why then, are amino acids “Glucogenic”? A. Misnomer; they are not B. They use glyoxalate pathway C. They make ketone bodies from Glc D. Enter TCA after loss of C as CO2 E. What is glucogenic? Diseases Fixing N2 N2 fixation is energy intensive Nitrogen fixation Considering the importance of N2 fixation, do you think Nitrogenase evolved early or late? A. Early B. Late C. I’m clicking this like a zombie. I don’t think in class Nitrogen fixation Then what might be one problem for Nitrogenase A. Carbon monoxide B. Carbon dioxide C. Oxygen D. Water vapor E. Temperature Legumes & nodules Symbiosis Fixing nitrogen Theoretically, why can’t we develop human symbionts for N2 fixation? A. No reason at all; we could! B. Bacteria would kill us C. Humans are aerobic D. We don’t have enough carbohydrates E. Excess NH3 – BO, dude!