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Transcript
Replication Learning Objectives: • To be able to describe the process of replication of the influenza and HIV virus • To be able to describe the course of infection, signs, symptoms and transmission if Influenza and AIDS Replication • Though the details of virus infection and replication vary greatly with host type, all viruses share 6 basic steps in their replication cycles. These are: – Attachment – Penetration – Uncoating – Replication – Assembly – Release • This is called lytic replication • The virus must first attach itself to the host cell. This is usually accomplished through special glycoprotiens on the exterior of the capsid, envelope or tail. • Next, penetration occurs, either of the whole virus or just the contents of the capsid. If the entire capsid enters, the genetic material must be uncoated to make it available to the cell's replication machinery. • Replication of genetic material takes place, as well as the production of capsid and tail proteins. • Once all of the necessary parts have been replicated, individual virus particles are assembled and released. • Release often takes place in a destructive manner, bursting and killing the host cell. Lysogenic • Some viruses, when they invade a cell do not undergo the full infective cycle. • They integrate themselves into the hosts DNA. • When the cell replicates, it also replicates the virus. • When the virus reassembles it re-enters the lytic phase, releasing new copies of the virus and rupturing the host cell Viral DNA injected into host cell Lytic cycle Lysis of cell Assembly of new viruses Lysogenic cycle Viral DNA integrated into hosts DNA • In the lytic phase virus particles infect host cells and are replicated. • In the lysogenic phase viral genetic material that has entered the host cell becomes incorportated in the cell and lies dormant. It is then passed on to the progeny of the infected cells. • Eventually, the lytic phase will start again, and cells that were never infected themselves, but carry the viral genetic material will begin to produce new virus particles. • What are the six steps in virus replication • attachment, penetration, uncoating, replication, assembly and release. • What is contained within the capsid of a virus? • The capsid contains the genetic material, either DNA or RNA, and sometimes enzymes. • How is the genetic material of viruses replicated? • Viruses have no replication capabilities themselves, so they must use the replication machinery of their host cells. Human Influenza Virus (type A,B or C) • A type of myxovirus (any of a group of RNA viruses including those that cause influenza and mumps) Influenza A viruses infect a wide variety of mammals, including man, horses, pigs, ferrets and birds • Influenza A viruses are a major human pathogen, associated with epidemics and pandemics. • Influenza B viruses infect humans only and cause disease, but generally not a severe as A types • Influenza C viruses infect humans only, but are not believed to cause disease. Method of transmission • Air-bourne droplets infection from the coughs and sneezes of infected individuals • Infected people are infected from a day before symptoms appear until a week afterwards Organ affected • The virus invades the epithelial cells of the upper respiratory tract and reproduces inside them, killing many cells in the process. • The dead cells increase the amount and thickness of the mucus produced which irritates the throat causing coughing. • Secondary bacterial infection is common Incubation time • Infected people are infected from a day before symptoms appear until a week afterwards • Symptoms usually lasts about 7-10 days, with the fever and aches improving after 2-3 days and the cough and sore throat lasting longer. • However, some people have symptoms which go on for much longer - weakness and depression sometimes lasts weeks. Symptoms • • • • • • • • • Fever (usually above 38°C) Chills Muscle aches and pain Severe headache Weakness and tiredness Loss of appetite Sore throat Cough, and Chest pain Treatment Most people got better on their own with simple relief for their symptoms. This includes: • Rest. The most important thing in flu is to give your body a chance to heal itself by resting. Lie down and sleep if possible. • Regular pain relief with aspirin, paracetamol or ibuprofen • Keep well-hydrated with plenty of warm fluids. • Remedies for sore throat, headache and blocked nose. Prevention • Vaccination is difficult due to genetic changes in the influenza virus • Vaccinations based on a variety of antigens are used to protect vulnerable groups • Prevention would require isolation of flu victims which is not practical. Human immunodeficiency virus (HIV) • HIV is a retrovirus (ie one that contains a single strand of RNA and the enzyme reverse transcriptase) Method of transmission • Through infected semen or vaginal secretions during sexual activity • Through infected blood in transfusions or contaminated needles • Across the placenta or in breast milk from infected mothers Organ affected • HIV in the blood attaches to cells that carry the ‘CD4’ antigen. These include T Lymphocytes and macrophage white blood cells. Incubation time • After entering the cell it becomes a provirus – that is a form of the virus which is capable of being integrated into the host genome. • It is replicated over a period of 8 – 10 years • Eventually the virus particles are reassembled and emerge into the blood, rupturing and killing the T cells in the process. This leaves the immune system compromised Symptoms • During the latent period there are no symptoms, but the individual is infectious. • After this period symptoms begin to appear that include: – Tiredness – Fever – Weight loss – Diarrhoea • This is followed by the more serious symptoms of AIDS AIDS • As the immune system is no longer functioning there is no defence against a variety of opportunistic infections • The most common are: – Kaposi’s sarcoma – TB – Pneumonia • All of these are usually fatal Treatment • There is, as yet, no cure or vaccination for HIV or AIDS • Drugs such as AZT can delay the onset of symptoms Prevention • Safe sex • Use only sterile needles • Screening of blood products What is a bacteriophage? b) What do they infect? c) Why are bacteriophages important industrially? d) How do they infect a cell? e) Why is the life cycle of a bacteriophage described as lytic? Do you consider viruses to be alive? Give reasons to justify your answer. Read the information on RETROVIRUSES. a) Give 2 features of all retroviruses b) Name a retrovirus c) Why are reverse transcriptase and retroviruses widely used in genetic engineering?