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Outline 3. Outline for Chapters Combining Replication and Pathogenesis
A.
History
B.
Classification of viruses within family
C.
Virion structure. Morphology, chemistry. Include illustrations such as
EM micrograph and diagram of particle and/or structure
D.
Genome structure and organization. Include summary diagram
E.
Stages of replication. Must include an overview diagram
1.
Mechanism of attachment
2.
Mechanism of entry and intracellular trafficking
3.
Uncoating
4.
Transcription
5.
Translation
6.
Replication of genomic nucleic acid. Discuss viral-encoded
replication proteins
7.
Late transcription and translation
8.
Assembly
9.
Release
10.
Effects on host cell. Ie., Apoptosis, lysis, inhibition or
stimulation of host processes, apoptosis, transformation, if
relevant.
F.
Pathogenesis and pathology. Include illustrations of typical pathology
and summary diagram of pathogenesis. Define mechanism
(molecular) if know. Include relevant natural host and experimental
systems. If appropriate, discussion of pathogens could incorporate
both natural and experimental infection or, in some cases, it may be
clearer to separate the two. In addition to text with the following
section, please provide diagrams that illustrate them.
1.
Entry into the host. What are the natural (and experimental)
portals of entry?
2.
Site of primary replication. What are the cells involved
(macrophages, epithelium, etc.) and what is know about this
stage (are receptors know? are the cell polarized? if so, is
entry apical or basal?)
3.
If virus spreads in the host, how does it? Is neural, blood
lymphatic, etc.? Please refer to old data if relevant (many of the
best studies are old)
4.
Cell and tissue tropism. Where does the virus arrive after
spread?
5.
Immune response. What are the data on early responses
(macrophages, NK cells, interferon, TNF, etc.) and later
responses (B and T cells)? Describe the mechanisms the virus
employs to avoid the immune response
6.
Release from host. Any data on efficiency, titers, mechanisms,
or virus release?
7.
Are there animal models or veterinary correlates that provide
useful clues to thinking about human disease?
8.
Virulence. Any data on relative capacity of closely related
strains to cause disease?
9.
G.
Persistence. Discussion of latency etc., when relevant
Epidemiology. Concepts and principles, not all examples
1.
Age
2.
Morbidity/Mortality
3.
Origin and spread of epidemics
4.
Prevalence and seroepidemiology
5.
New epidemiologic approaches (including molecular
approaches, if relevant)
H.
Clinical features. Outline clinical course. A summary diagram may be
helpful
I.
Diagnosis.
1.
Differential
2.
Laboratory
J.
K.
Prevention and control.
1.
Treatment
2.
Vaccines
3.
Other – insect control, etc.
Perspective. Identify and discuss important issues and problems for
future study of this virus. Suggested length: 250-500 words.
Note: We encourage additional figures beyond the required ones.