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Supplemental 4: C. elegans Rbx-1 is unlikely a component of the RPM-1/FSN-1 protein complex a b rbx-1 RNAi treatment (against ZK287.5) - + N2 N2 IP antibody juIs58 56 kDa N2 juIs58; juIs58 fsn-1 N2 juIs58; juIs58 fsn-1 FSN-1 Nonspecific 30 kDa 30 kDa 20kDa 20 kDa RBX-1 RBX-1 b-tubulin 56 kDa b-tubulin 10% lysate input 56 kDa Panel a: A polyclonal antibody raised against yeast RBX1 (provided by M. Tyers, University of Toronto) was tested against the C. elegans total protein lysates. Adult wild-type animals (N2) were fed with bacteria expressing dsRNA against ZK287.5 (the C. elegans homologue of RBX1) for three days, and the protein lysates were probed with RBX1 antibodies at 1:2000 dilution. Compared with lysates from untreated animals, a band corresponding to the predicted molecular weight of C. elegans RBX-1 protein was consistently decreased/abolished in three sets of experiments. This band most likely corresponds to the C. elegans RBX-1 protein. Panel b: Immunoprecipition from wild-type (N2), RPM-1::GFP (juIs58) and fsn-1;RPM-1::GFP(juIs58) lysates by antibodies against GFP. FSN-1 specifically associates with RPM-1::GFP, while RBX-1 does not. The same condition as in Figure 2 was used for the co-immunoprecipitation experiment.